해양심층수의 농업 분야 활용

홍성유(Sung Yu Hong),우천석(Cheon Seok Woo),강원희(Won Hee Kang) 한국원예학회 2005 한국원예학회 학술발표요지 Vol.2005 No.5

육묘 시 해양심층수의 관수 방법이 유묘의 생장에 미치는 영향

홍성유(Sung Yu Hong),윤병성(Byeong Sung Yoon),강원희(Won Hee Kang) (사)한국생물환경조절학회 2006 생물환경조절학회지 Vol.15 No.2

토마토 초장의 경우 심층수처리는 대조구에 비해 두 상관수는 50%, 저면관수는 58% 감소하였고, 표층수 처리구에서는 두상관수는 49%, 저면관수는 56% 감소하였으며, NaCl 처리구에서는 두상관수는 47%, 저면 관수는 57% 감소하여 저변관수 처리방법이 도장억제에 더 효과적이었다. 지상부 생체중의 경우 두장관수보다 저면관수에서 더 감소하는 경향을 보였으나 뚜렷한 차이가 나타나지 않았다. 지하부 생체중 대조구에 비하여 두상관수는 38%정도 감소한 반면 저면관수는 49% 정도 감소하여, 저면관수에 의해 생육이 현저히 억제되는 결과를 보였다. 지상부와 지하부의 건물중은 생체중과 비슷한 경향을 나타냈다. 이와 같은 결과는 염에 의한 뿌리생육의 저하 때문인 것으로 판단된다. 경경은 대조구에 비해 감소하였지만 처리 방법 간에 뚜렷한 차이를 보이지는 않았다. T/R률에 비추어 보면 두상관수 처리에서 묘소질이 더 뛰어났으나, 묘의 충실도(compactness)는 처리구간 차이는 나타나지 않았다. 토마토의 경우 초장의 억제정도나 묘의 충실도에 비춰보아 저면관수 방법이 도장억제에 더 효과적이라 사료된다. 오이의 경우 처리 농도의 증가에 따라 초장, 생체중, 건물중, 경경, 엽면적이 감소하였다. 저면관수가 두상관수에 비해 도장억제 효과와, 생육억제가 좀 더 뚜렷이 나타나기는 하였으나 두 처리간의 유의적 차이는 나타나지 않았다. Overhead and sub-irrigation of deep sea water to tomato seedlings reduced the height as 50% and 58% than control plants. In the same treatment with surface sea water and NaCl water, the reduced rate in tomato seedlings' height were 49% and 56% in overhead irrigation, and 47% and 57% in sub-irrigation, respectively. Most effective method for the inhibition of the growth of the seedling was sub-irrigation method, which supplied water through the roots. No significant difference was observed on fresh weight of the upper part of tomato and cucumber seedlings, though the sub-irrigation reduced the fresh weight than the overhead irrigation. The reduced rate of fresh weight of seedlings by overhead irrigation was by 38% and sub-irrigation by 49% as compared to control. Similarly dry weight of upper and under soil parts of seedlings showed same trend of results thereof as fresh weight. This result can be traced to reduction of growth caused by salts in the water. In stem diameter of seedlings no significant difference was observed between two irrigation methods, even though both deep sea and NaCl water reduced stem diameter, as compared to control water. Overhead irrigation can be chosen by seedling producers because of better seedling quality by using TIR ratio. Seedling compactness were not noticed in both the overhead and sub-irrigation. Sub-irrigation was found more effective method for the inhibition of height and compactness of tomato seedlings. Higher the concentration of NaCl, deep sea, and surface sea water, lesser the growth in height, fresh and dry weight, stem diameter, and leaf area was obtained. No significant difference was found, though sub-irrigation suppress the growth of seedlings.

효소를 이용한 말론산의 새로운 측정법

김유삼,홍성유,강상원,장정순 ( Yu Sam Kim,Seung Yoo Hong,Sang Won Kang,Chung Soon Chang ) 생화학분자생물학회 1993 BMB Reports Vol.26 No.3

A novel enzymatic assay of malonate concentration was established by using malonyl-CoA synthetase from Rhizobium trifolii. By this simple method malonate could be determined up to 0.1 mM acculately and rapidly. It was also known to be useful method for the diagnosis of methyl-malonic acidaemia.

효소를 이용한 말론산의 새로운 측정법

김유삼,홍성유,강상원,장정순,Kim, Yu-Sam,Hong, Seung-Yoo,Kang, Sang-Won,Chang, Chung-Soon 생화학분자생물학회 1993 한국생화학회지 Vol.26 No.3

Rhizobium tnifolii로부터 정제한 말론산-CoA 연결효소를 이용하여 0.1mM 말론산의 농도까지 정확, 신속, 간편하게 측정할 수 있는 새로운 효소적 방법을 확립하였다. 또한 이 방법은 이미 알려진 메칠말론산 과다배설증 환자의 진단에도 효과적으로 응용될 수 있음을 알 수 있었다. A novel enzymatic assay of malonate concentration was established by using malonyl-CoA synthetase from Rhizobium trifolii. By this simple method malonate could be determined up to 0.1 mM acculately and rapidly. It was also known to be useful method for the diagnosis of methyl-malonic acidaemia.

Bacillus subtilis Natto가 생산하는 Nattokinase의 항혈전 및 피브린 용해능 효능평가

이다령(Da-Lyung Lee),홍성유(Sung-Yu Hong),장양수(Yang-Su Jang),장형욱(Hyung-Wook Jang),맹창재(Chang-Jae Maeng),유철배(Chul-Bae Yoo),백대헌(Dae-Heoun Baek) 한국생물공학회 2012 KSBB Journal Vol.27 No.6

We previously reported that Ultra nattokinase<SUP>ⓡ</SUP> showed high fibrinolytic activity and revealed antithrombotic effect in rat blood plasma based on its ability to suppress collagen-induced platelet aggregation. This research was carried out to verify the clot lysing activity and blood flow enhancing effects of Ultra nattokinase<SUP>ⓡ</SUP> via monitoring and comparing the antithrombotic effects in rat artery between oral administration of Ultra nattokinase<SUP>ⓡ</SUP> and maltodextrin. SD rats were fed with 1.11 mg/kg of Ultra nattokinase<SUP>ⓡ</SUP> for 4 weeks. The effect on arterial thrombosis was then evaluated using an antithrombotic model after induction by FeCl₃. Detected fibrinolytic activity was proportional to the content of Ultra nattokinase<SUP>ⓡ</SUP> and statistical extents of the antithrombotic activity was enhanced strongly twice rather than control group. The PT and the aPTT, however, showed only a small difference between two groups. The results suggest that Ultra nattokinase<SUP>ⓡ</SUP> can effectively treat thromboembolism and enhance blood flow, and that Ultra nattokinase<SUP>ⓡ</SUP> can also prevent venous occlusion by aiding clot lysis.

Effects of Deep Sea Water Treatment on Plug Seedling

강원희(Won Hee Kang),우천석(Cheon Seok Woo),홍성유(Sung Yu Hong) 한국원예학회 2004 한국원예학회 학술발표요지 Vol.2004 No.10

품종별 들깻잎 열수추출물의 caffeic acid와 rosmarinic acid의 함량 및 항산화력 비교

이현순(Hyun-Sun Lee),이현아(Hyun Ah Lee),홍충의(Chung-Oui Hong),양성용(Sung Yong Yang),홍성유(Sung Yu Hong),박상률(Sang Yul Park),이호정(Ho Jung Lee),이광원(Kwang-Won Lee) 한국식품과학회 2009 한국식품과학회지 Vol.41 No.3

본 연구는 품종별 들깻잎의 기능성 건강식품에 항산화력 측정과 생리활성물질인 caffeic acid와 rosmarinic acid의 함량 분석을 통하여 항산화관련 기능성 식품소재에 적합한 들깻잎의 품종을 찾기 위하여 수행하였다. 분쇄한 각 품종별 들깻잎을 환류 추출하여 여과한 후 동결 건조한 물질을 각 실험의 시료로 사용하였다. 총 폴리페놀 함유량을 측정한 결과, 보라들깨와 동글2호가 각각 69.4 g GAE/kg DM, 61.8 g GAE/kg DM로 높은 수치를 나타내었다. 그리고 항산화 실험 결과, FRAP에서는 보라들깨가 796mM FeSOsub{4}?7H2O/g DM로 가장 높은 항산화력을 가진 것으로 나타났으며, 그 뒤로 동글2호가 748 mM FeSOsub{4}?7H2O/g DM으로 높았고, DPPH 결과에서는 SCsub{50}값이 동글2호가 241 μg DM/mL로 가장 항산화력이 높았고, 그 다음으로 261 μg DM/mL로 보라들깨였다. 플라보노이드 함유량 측정에서는 늘보라들깨가 64.5 g QE/kg DM로 가장 높은 결과를 나타내었다. HPLC를 통하여 이미 잘 알려진 항산화제인 caffeic acid와 rosmarinic acid의 함유량을 측정한 결과, caffeic acid는 보라들깨에서, rosmarinic acid는 동글 2호에서 가장 높은 함유량을 나타내었다. 즉, 이 종합적인 비교를 통하여 항산화능력과 생리활성물질 함유량 측면에서 보라들깨와 동글2호가 가장 적합한 품종이라고 결론지을 수 있다. The principal objective of this investigation was to identify adequate species for the harvest of Perilla frutescens leaves, which provide profound antioxidant activities, and harbor abundant caffeic acid and rosmarinic acid. Namchun, Donggeul-2, Bora, Sae-bora and Neul-bora variants of the plant were assessed herein. In this study, we evaluated the antioxidant effects of these plants, and utilized an HPLC system to verify their caffeic acid and rosmarinic acid contents. Dried Perilla frutescens leaves were boiled at a temperature of 100℃ for three hours, and were lyopholized in a freezedryer. The extracts were then processed in order to confirm their antioxidant activities via 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical-scavenging activity assay, ferric-reducing antioxidant power (FRAP) assay, total flavonoid content and total polyphenol content assays. According to the observed antioxidant results of the five tested species of Perilla frutescens leaves, Bora and Donggeul-2 were shown to have more potent antioxidant activities than the other tested variants. The scavenge 50% of DPPH radical (SCsub{50} of DPPH) values were 241 μg DM/mL in Donggeul-2 and 261 μg DM/mL in Bora. Based on the results of the FRAP assay, the Bora variant showed a value of 796 mM FeSOsub{4}?7Hsub{2}O/g DM, and Donggeul-2 exhibited a value of 748 mM FeSOsub{4}?7Hsub{2}O/g DM, of which the total polyphenol contents were measured as 69.4 g GAE/kg DM and 61.8 g GAE/kg DM, respectively. Moreover, the Bora variant had the highest level of caffeic acid, and Donggeul-2 showed the highest rosmarinic acid content among the tested samples (0.87 mg/100 g wet base and 121 mg/100 g wet base, respectively). According to the results of this experiment, we selected two species, Bora and Donggeul-2, which were both verified to contain adequate and favorable antioxidant activities.

유색칼라 상자재배 시 적정 관수 방법

김영진(Young Jin Kim),최강준(Kang Jun Choi),홍성유(Seong Yu Hong),변선배(Sun Bae Byon),박영식(Young Sik Park),임상현(Sang Hyun Lim) 한국원예학회 2019 한국원예학회 학술발표요지 Vol.2019 No.10