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김형섭 ( Hyung Sub Kim ),김용순 ( Yong Soon Kim ),임채형 ( Chae Hyung Lim ),곽승준 ( Seung Jun Kwack ),조영래 ( Young Rae Cho ),강미선 ( Mi Sun Kang ),오재호 ( Jae Ho Oh ),조완섭 ( Wan Seob Cho ),한범석 ( Bum Seok Han ),김승희 ( S 한국동물실험대체법학회 2007 동물실험대체법학회지 Vol.1 No.2
To compare the responsiveness of nephrotoxicity parameters, mercuric chloride(HgCl2) was administered intraperitoneally at the doses of 0.2, 0.6 and 1.0 mg / kg for different exposure periods(1, 3 and 7 days) using F344N rats. We compared several parameters resulted from animal study(body weight change, organ weight), clinical pathological(serum and urine chemistry), histopathological study and toxicogenomic study. Animal body weight significantly decreased and kidney weight(relative) increased in a dose-dependent manner. Serum chemistry analysis showed that BUN and creatinine were relatively one of nephrotoxicity biomarkers against HgCl2 and serum chemistry analysis was more sensitive than urine analysis. In the histopathological study, dose and time-dependency was clearly observed. Cellular swelling was observed in the low dose(0.2 mg / kg) group(1 day treatment) and focal inflammation including cellular swelling was observed in the high dose group. In the DNA microarray analysis, gene expression profiles were analysed in the 1-day and 3-days treatment group. Taken together, the responsiveness of individual parameter(body weight change, organ weight, clinical pathology, histopathology and DNA microarray) was different. This study suggests that these considerations should be involved in the analysis of toxicity study data as well as toxicogenomic study could provide a useful information to reduce the number of experimental animals used in the repeated toxicity study.
김형섭 ( Hyung Sub Kim ),김용순 ( Yong Soon Kim ),임채형 ( Chae Hyung Lim ),곽승준 ( Seung Jun Kwack ),조영래 ( Young Rae Cho ),강미선 ( Mi Sun Kang ),오재호 ( Jae Ho Oh ),조완섭 ( Wan Seob Cho ),한범석 ( Bum Seok Han ),김승희 ( S 한국동물실험대체법학회 2007 동물실험대체법학회지 Vol.1 No.2
To compare the responsiveness of nephrotoxicity parameters, mercuric chloride(HgCl2) was administered intraperitoneally at the doses of 0.2, 0.6 and 1.0 mg / kg for different exposure periods(1, 3 and 7 days) using F344N rats. We compared several parameters resulted from animal study(body weight change, organ weight), clinical pathological(serum and urine chemistry), histopathological study and toxicogenomic study. Animal body weight significantly decreased and kidney weight(relative) increased in a dose-dependent manner. Serum chemistry analysis showed that BUN and creatinine were relatively one of nephrotoxicity biomarkers against HgCl2 and serum chemistry analysis was more sensitive than urine analysis. In the histopathological study, dose and time-dependency was clearly observed. Cellular swelling was observed in the low dose(0.2 mg / kg) group(1 day treatment) and focal inflammation including cellular swelling was observed in the high dose group. In the DNA microarray analysis, gene expression profiles were analysed in the 1-day and 3-days treatment group. Taken together, the responsiveness of individual parameter(body weight change, organ weight, clinical pathology, histopathology and DNA microarray) was different. This study suggests that these considerations should be involved in the analysis of toxicity study data as well as toxicogenomic study could provide a useful information to reduce the number of experimental animals used in the repeated toxicity study.