고지방 식이 비만 모델 마우스의 체중 및 혈장 지질 대사에 미치는 한방보조식품의 영향

최혜민,석장미,양재하,김상찬,김미려,Choi, Hye-Min,Suk, Jang-Mi,Yang, Chae-Ha,Kim, Sang-Chan,Kim, Mi-Ryeo 대한한의학방제학회 2009 大韓韓醫學方劑學會誌 Vol.17 No.2

Objective : Recently, obesity has increased at an alarming rate and is now a worldwide health problem. Natural dietary supplement, $CharmSlim^{(R)}$(C), is mixed pills, which consists of beans, black sesames, kelps, onions, adlay seeds, anchovies, pyogo mushrooms, green tea. Our study was performed to determine the weight-loss effects of natural dietary supplement, based on natural ingredients, in mice fed high-fat diet with an abundance of carbohydrates. Methods : Four groups of male ICR mice were fed different diets during 6 weeks: normal diet(NOR), high-fat (15%, w/w) diet(HF), high-fat supplemented with natural dietary supplement powdered 5%(C5) and 10%(C10) groups. We measured the changes of food intake, body weight and adipose tissues weights. Also we examined levels of lipid profiles in serum. Results : Body weight, liver weight and food efficiency ratio were significantly higher in the HF group than in NOR group. After 6 weeks of treatment, body weight, liver weight and adipose tissues weights (epididymal, perinephric, visceral and BAT) were significantly decreased in the C10 group when compared to the control HF group. The C10 group had markedly lower serum levels of total cholesterol and triglyceride when compared with HF group. Conclusion : These results demonstrate that this dietary supplement can suppress the increased lipid profiles, body weight and fat gains, thus could be considered as an effective adjunct in the treatment of obesity.

정삼투막에 의한 붕산함유 방사성 폐액 처리를 위한 pH 및 이온강도 영향

최혜민,황두성,이근우,문제권,Choi, Hye-Min,Hwang, Doo-Seong,Lee, Kune-Woo,Moon, Jei-Kwon 한국방사성폐기물학회 2013 방사성폐기물학회지 Vol.11 No.3

일반적으로 역삼투압 공정에 의해 중성 pH 조건에서 40~90%의 붕소를 회수할 수 있다.정삼투공정은 새로운 선진 기술로 폐수처리 및 담수화 분야에서 관심이 높아지고 있다. 본 연구의 목적은 정삼투공정으로 방사성 액체 폐기물의 붕소제거 가능성을 고찰하고자 하는데 있다. 액체폐기물에서의 붕소 제거를 위한 정삼투공정의 성능을 평가하기 위해 pH, 삼투압, 용액의 이온강도 등을 고려하였다. 폐액의 붕산분리능을 좌우하는 주 조업변수인 pH 조건은 pH 7 이하에서 80% 이상의 붕소분리를 달성할 수 있었다. 약 1,000 mg/L 정도의 염농도에서는 막의 물의 플럭스는 거의 영향이 없었으나 붕소 투과율은 약간 감소하는 경향을 나타내었다. 공급액 내 붕소 농도 증가에 따라 붕소플럭스는 선형적으로 증가하였으며, 붕소의 투과율은 약 80% 정도로 일정하였다.

65종 한약처방이 3T3-L1 지방전구세포의 지방 분화에 미치는 효능 비교 연구

최혜민,유병우,김민주,김정옥,Choi, Hye-Min,Yu, Byung-Woo,Kim, Min-Ju,Kim, Jung-Ok 한방비만학회 2020 한방비만학회지 Vol.20 No.2

Objectives: To expand and provide information on the efficacy of herbal medicines, anti-obesity effects were evaluated. In many studies, plant-derived components with anti-obesity efficacies have been investigated for their potential inhibitory effects on 3T3-L1 preadipocyte cells. The purpose of this study was to investigate the anti-obesity effects of 65 herbal medicine in 3T3-L1 preadipocyte cells. Methods: Preferentially, 3T3-L1 cells were treated with 65 herbal medicines (500 ㎍/mL) during differentiation for 8 days. Next, 3T3-L1 cells were treated with selected herbal medicines at concentrations ranging from 50 to 200 ㎍/mL during differentiation for 8 days. The accumulation of lipid droplets was determined by Oil Red O staining. The expressions of genes related to adipogenesis were measured by reverse transcription polymerase chain reaction and Western blot analyses. Results: Among the 65 kinds of herbal medicines, 13 herbal medicines that been shown to be effective against the accumulation of lipid droplets were selected. Finally, selected Banhasasim-tang and Samhwangsasim-tang showed inhibitory activity on adipocyte differentiation at 3T3-L1 preadipocytes without affecting cell toxicity. In addition, Banhasasim-tang and Samhwangsasim-tang significantly reduced the expression levels of several adipocyte marker genes including peroxisome proliferator activated receptor-γ and CCAAT/enhancer binding protein-α. Conclusions : These results suggest that the ability of Banhasasim-tang and Samhwangsasimtang has inhibited overall adipogenesis and lipid accumulation in the 3T3-L1 cells. Banhasasim-tang and Samhwangsasim-tang may be a promising medicine for the treatment of obesity and related metabolic disorders.

유지 투석 개시시 혈청 Cystatin C의 의의: 혈청 Creatinine과 계산된 사구체 여과율과의 비교

최혜민 ( Hye Min Choi ),김혜원 ( Hye Won Kim ),이재원 ( Jae Won Lee ),현영율 ( Young Youl Hyun ),박상원 ( Sang Won Lee ),이지은 ( Ji Eun Lee ),권영주 ( Young Joo Kwon ),표희정 ( Heui Jung Pyo ) 대한신장학회 2007 Kidney Research and Clinical Practice Vol.26 No.1

목적: 혈청 cystatin C (Cys-C)는 혈청 creatinine (Cr)과는 달리 비신성 요인에 의한 영향이 적어 사구체 여과율을 더 정확하게 반영할 수 있을 것이라는 보고가 많이 있었다. 특히 조기 신기능 저하 또는 신이식 환자에서 Cr보다 신기능을 예민하게 반영한다는 연구 결과들이 발표된 바 있으나, 말기 신질환 환자에서 Cys-C에 대한 연구는 거의 없다. 저자들은 말기 신부전 환자에서 투석 개시시 혈청 Cys-C를 측정한 후 혈청 Cr, 24시간 Cr 청소율 (G (Ccr)) 및 C&G formula, MDRD formula로 계산한 사구체 여과율 (각각 G (C&G), G (MDRD1), G (MDRD2))과 비교하고, 비신성 요인의 영향을 살펴보며, 혈청 Cys-C를 투석 개시시 객관적 혈청 지표로 활용할 수 있는지 알아보고자 하였다. 방법: 말기 신부전 환자 163명을 대상으로, 투석 개시시 혈청 Cys-C 및 Cr을 측정하였다. 이후 Pearson 상관 분석으로 G (Ccr), G (C&G), G (MDRD1), G (MDRD2)와의 상관계수를 구하였으며, 다중 회귀 분석을 이용하여 성별, 연령, 체중, 당뇨병성 신증 유무와 같은 비신성 요인의 영향을 확인해 보았다. 또한 ROC curve를 통해 혈청 Cys-C 수치로 응급 혈액 투석 가능성을 예측할 수 있는지 검토하였다. 결과: 1/Cys-C는 G (Ccr), G (C&G), G (MDRD1), G (MDRD2)와 의미 있는 상관 관계를 보였다. 다중 회귀분석에서 혈청 Cr은 고령, 저체중, 당뇨병성 신증과 각각 독립적으로 유의한 음의 관계가 있었고, 혈청 Cys-C는 성별, 체중, 당뇨병성 신증 여부와는 유의한 관계가 없었으나 연령이 증가함에 따라 감소하는 경향을 보였다. 투석 개시시 요독증상이 심해 응급 혈액 투석을 시행한 경우를 기준으로 그려본 ROC 곡선에서 아래 영역 면적은 혈청 Cr, Cys-C가 각각 0.684±0.043, 0.644±0.044로서, 응급 혈액 투석을 예견하며 조속한 투석개시를 요구할 수 있는 혈청 표지자로서 Cys-C는 Cr보다 우월하지 않았다. 결론: Cys-C는 말기 신질환 환자에서 투석 개시시 Cr 을 기초로 한 사구체 여과율과 연관 관계가 있었다. 혈청 Cys-C는 체중, 성별에 대해선 혈청 Cr보다 영향을 받지 않았으나, 독립적으로 연령의 영향을 받았다. 이상으로 혈청 Cys-C는 투석 개시시 및 응급 혈액 투석을 예측하는 혈청 표지자로서 혈청 Cr 보다 우월하지 않았다. Purpose: Cystatin-C is produced at a constant rate, and has been known to be unaffected by non-renal factors. However, there are limited data on its superiority to serum creatinine as a marker of renal function in ESRD population. The aims of our study were to compare serum cystatin-C and serum creatinine with estimated GFR in ESRD patients at the initiation of dialysis, whether the non-renal factors may influence on serum cystatin-C levels, and whether serum cystatin-C may be a useful marker of the start of dialysis. Methods: This study was cross-sectional about 163 ESRD patients. We measured serum cystatin-C and serum creatinine levels at the initiation of dialysis, and determined GFR from 24 hour urine collection [G (Ccr)], Cockcroft-Gault [G (C&G)], and the modification of diet in renal disease [G (MDRD)] formula. We considered age, gender, body weight and diabetic nephropathy as non-renal factors. Results: The mean serum cystatin-C was 5.0±0.9 mg/dL, serum creatinine 11.4±5.9 mg/dL, G (Ccr) 5.0±2.9 mL/min/1.73m2, G (C&G) 7.5±3.1 mL/min/1.73m2, G (MDRD1) 5.7±2.9 mL/min/1.73m2, and G (MDRD2) 5.5±2.5 mL/min/1.73m2. We found significant correlation between estimated GFR and serum cystatin-C. However, comparing to serum creatinine, serum cystatin-C had no merits in estimating renal function and in predicting urgent hemodialysis. In the multivariate linear regression models, serum cystatin-C had no significant correlation with gender, body weight, and diabetic nephropathy, but decreased with the age. Conclusion: Serum cystatin-C is not superior to serum creatinine for estimating renal function and predicting urgent hemodialysis in ESRD patients. Besides, serum cystatin-C seems to be influenced by non-renal factors, age.

보중익기탕 (補中益氣湯)의 한국, 중국, 일본 처방에 대한 항염증 및 항산화 효과 비교 연구

최혜민 ( Hye Min Choi ),김희훈 ( Hui Hun Kim ),이화동 ( Hwa Dong Lee ) 대한본초학회 2014 大韓本草學會誌 Vol.29 No.1

Objectives : Bojungikgitang (BJT), the Oriental medical prescription has been traditionally used about improvement of immune response and infective disease at Asian nation. In this study, we has compared about the anti-inflammatory and antioxidative effects on BJT of three countries including Korea (Korean Traditional Medicine, KTM), China (Traditional Chinese Medicine, CTM) and Japan (Japanese Traditional Medicine, JTM). Methods : We has basically using LPS-stimulated RAW 264.7 cells. The expression of these inflammatory mediators has measured using enzyme-linked immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR). Also, free radical scavenging assay has tested for anti-oxidative activity as well as the contents of total flavonoid and polyphenol. Results : As a result, we were founded the inhibitory effects of BJT (KTM, CTM, JTM) on LPS-induced production of NO, TNF-α and IL-6 as well as the anti-oxidative activities. Especially the KTM was most effective in anti-inflammatory and anti-oxidative activities. Conclusions : These results indicate that BJT (KTM, CTM, JTM) has a good anti- inflammatory and anti-oxidative effects. But, there were degree of effects on between pharmacopoeia of the countries. Thus, further study is required that find appropriate methods for extracting as well as establish of standardized processes in order to improve the quality of BJT (KTM, CTM, JTM) as an anti-inflammatory and anti-oxidative agent for treatment of inflammatory diseases.

혈액투석에서 복막투석으로 전환한 환자에서 발생한 특발성 적혈구 증가증

최혜민 ( Hye Min Choi ),현영율 ( Young Yul Hyun ),부창수 ( Chang Su Boo ),성수아 ( Su Ah Sung ),조상경 ( Sang Kyung Jo ),조원용 ( Won Yong Jo ),김형규 ( Hyung Kyu Kim ),이지혜 ( Ji Hye Lee ) 대한신장학회 2005 Kidney Research and Clinical Practice Vol.24 No.3

오적산 (五積散)의 고지방식이 마우스 지방축적억제 및 3T3-L1지방세포에서의 비만 조절 기전에 관한 연구

최혜민 ( Hye-min Choi ),문성옥 ( Sung-ok Moon ),이희현 ( Hee-hyun Lee ),이화동 ( Hwa-dong Lee ) 대한본초학회 2015 大韓本草學會誌 Vol.30 No.4

Objectives: Ojeok-san (OJS), an oriental herbal formula, has been used in Asian countries including Korea, China and Japan to treat the common cold and illnesses including fatigue and gastrointestinal disorders. The purpose of this study was to examine the anti-obesity effect and molecular mechanism of OJS, on adipogenesis in 3T3-L1 cells. Also, the effects of OJS in obese mice fed a high-fat diet on adiposity were examined. Methods: Preferentially, we analyzed the component of OJS and measured the stability of its component in OJS according to study periods using high performance liquid chromatography (HPLC). In vitro, 3T3-L1 cells were treated with OJS (50 to 200 μg/mL) during differentiation for 8 days. The accumulation of lipid droplets was determined by Oil Red O staining. The expressions of genes related to adipogenesis were measured by RT-PCR and Western blot analyses. For anti-obesty effect in vivo, we experimented for 8 weeks with four group (normal diet (CON), high-fat diet (HF), high-fat diet with OJS (HF+OJS) and high-fat diet with Bang-pung-tong-sung-san (HF+BTS) in comparison group HF+OJS). Results: OJS showed inhibitory activity on adipocyte differentiation at 3T3-L1 preadipocytes without affect cell toxicity as assessed by measuring fat accumulation and adipogenesis. In addition, OJS significantly reduced the expression levels of several adipocyte marker genes including proliferator activated receptor-γ (PPAR-γ) and CCAAT/enhancer-binding protein-α (C/EBP-α). Also OJS-administered mice showed significant inhibitory of body weights and abdominal adipose tissue weights. Conclusions: This study showed that traditional medicine OJS has an anti-obesity effect in vitro and in vivo. Thus, OJS could be developed as a supplement for reduction of body weight gain induced by an obesity.

한약재 함유 헤어토닉 및 식품이 C57BL/6 마우스의 탈모 모델에서 모발성장에 미치는 영향

최혜민 ( Hye Min Choi ),황수정 ( Su Jung Hwang ),이진상 ( Jin Sang Lee ),도은주 ( Eun Ju Do ),김무영 ( Mu Young Kim ),김미려 ( Mi Ryeo Kim ) 대한본초학회 2011 大韓本草學會誌 Vol.26 No.1

Objective: This study was carried out to investigate effect of korean medicinal herbs-included hair tonic (MHT) and food (MF) on hair growth in an alopecia model of C57BL/6 mice. Methods: Six-weeks old mice were given laboratory rodent chow diet for 1 week. Then, we used a depilatory for hair removal on mice. The next day, mice were randomized and separated in 3 groups of 6 mice; normal group (N, vehicle epidermal application+normal diet-treated group), positive control group (C, 3% minoxidil sulfate epidermal application+normal diet-treated group), and the treatment group (T, HT epidermal application+ MF diet-treated group). The hair regrowth was determined by photograph, which was taken at 7, 14 and 21 days. And scoring indices, hair density and hair thickness, were evaluated by Folliscope at same day. In addition, the hair regrowth was analyzed insulin-like growth factor (IGF-1) in the dorsal skin of mice. Results: As a result, not only external appearance but also hair density and hair thickness on dorsal skin were increased more in C, T groups than in N group at 21 days with mixed treatment. Therefore, distinct regrowth pattern is appeared by treatment of herbal tonic and natural food for 21 days. Also, the expressions of IGF-1 on dorsal skin were higher in C, T groups than in N group. Conclusion: These results suggested that this herbal hair tonic and natural food has hair growth promoting activities and may be useful for treatment of bald or alopecia.

리포익산 나노 입자의 식욕 억제에 대한 고분자의 영향

최혜민 ( Hye Min Choi ),박철호 ( Chul Ho Park ),이기업 ( Ki Up Lee ),박중열 ( Joong YeoI Park ),고은희 ( Eun Hee Koh ),김현식 ( Hyoun Sik Kim ),이종휘 ( Jong Hwi Lee ) 韓國藥劑學會 2007 Journal of Pharmaceutical Investigation Vol.37 No.6

가미소요산의 새로운 제형에 대한 항산화 활성 및 항염증 효능평가

최혜민 ( Hye-min Choi ),김세진 ( Se-jin Kim ),김인수 ( In-su Kim ),이지범 ( Ji-beom Lee ),김종범 ( Jong-beom Kim ),문성옥 ( Sung-ok Moon ),이화동 ( Hwa-dong Lee ) 대한본초학회 2016 大韓本草學會誌 Vol.31 No.6

Objectives : Gamisoyosan (GMS) is a useful prescription for treating insomnia, dysmenorrhea and infertility induced by a stress. Also, GMS has been used traditionally to improve systemic circulation and biological energy production. The purpose of this study was to assess the anti-oxidant activity and anti-inflammatory effects of Gamisoyosan Formulation (Soft extract, GMS-SE). Methods : The biological activities such as anti-oxidant and anti-inflammatory effects were measured through cell line-based in vitro assay. We investigated the anti-oxidant properties of GMS-SE on the 1,1-diphenyl- 2-picryhydrazyl (DPPH) radical, contents of total flavonoid and polyphenol. GMS-SE compared to butyl hydroxy anizole (BHA). Furthermore, based on this result the anti-inflammatory effects of GMS-SE have verified by mechanism from LPS- treated Raw264.7 macrophages. Results : The anti-oxidant activities of GMS-SE increased markedly, in a dose-dependent manner. The GMS-SE showed significant scavenging activity (GMS-SE 500 ㎍/㎖ : 32.77±1.65%, GMS-SE 1000 ㎍/㎖ : 45.06±1.04% and BHA 100 ㎍/㎖ : 39.25±2.41% for DPPH assay). and, The total phenolic compound and flavonoids contents of GMS-SE were 73.93±6.87 ㎍/㎎ and 698.75±6.78 ㎍/㎎. GMS-SE which is LPS has diminished in the LPS-induced release of inflammatory mediators (NO, iNOS, COX2 and PGE2) and pro-inflammatory cytokines (TNF-α, IL-6 and IL-1β) from the RAW264.7 macrophages. Moreover, GMS-SE inhibited the activation of phosphorylation of p38 and ERK MAPKs by induced LPS. Conclusion : The present results indicate that GMS-SE has an anti-oxidant and anti-inflammatory properties, therefore may be beneficial in diseases which related to oxidative stress-mediated inflammatory disorders.