백서에서 열량 영양소 배분과 교차가 인슐린 분비능과 인슐린 저항성에 미치는 영향

최수봉(Soo Bong Choi),박봉안(Bong An Park),염세진(Sea Jin Yeom),박선민(Sun Min Park) 대한내과학회 2002 대한내과학회지 Vol.62 No.2

N/A Background: In Korea, the prevalence of type 2 diabetes has been rapidly increased in parallel with the westernization of eating behavior, which can be potential contributors for insulin resistance. We determined whether Western diet (WD), Korean diet (KD), Western-Korean exchange diet (WDK), Korean-Western exchange diet (KDW) and control diet (CD) altered insulin secretion and insulin resistance in weaning male Sprague Dawley rats. Methods : Weaning rats were provided by KD (77 En% of starch, 5 En% of corn oil and 13 En% of gluten plus 5 En% of casein), WD (42 En% of starch, 40 En% of butter and 18% of casein) or CD (62 En% of starch, 20 En% of corn oil and 18% of casein) for 21 weeks. At the 13th week, rats in the WDK and KDW groups exchanged the diets from WD to KD and KD to WD, respectively, and they continued on the exchanged diets for 9 weeks. Results: Fasting serum glucose levels were lower in KD and WDK than any other groups. Insulin secretion was higher by 2.2±0.4 folds in WD and 1.9±0.3 folds in KDW than KD at baseline. In hyperglycemic clamp, insulin secretion to maintain 11.1 mmol/L of serum glucose levels showed the same results as the baseline. Whole body glucose disposal rates were highest in KD among all groups. Glycogen deposits in soleus and quadriceps muscles were highest in KD among all groups, and their triglyceride contents were lowest. GLUT4 contents and fraction velocity of glycogen synthase were highest in KD in soleus muscles. Conclusion: Westernized high fat diets increased insulin resistance, and insulin secretion increased to overcome insulin resistance. Increased insulin resistance was due to increased triglyceride and decreased glycogen, GLUT4 and glycogen synthase contents in muscles.(Korean J Med 62:194-203, 2002)

흡연이 건강한 젊은 남자의 체내 산화 상태에 미치는 영향

최수봉(Soo Bong Choi),박선민(Sun Min Park) 대한내과학회 1998 대한내과학회지 Vol.55 No.5

N/A Objectives: It has been reported that cigarette smoking increases free radical generation, which can also increase lipid peroxides and deplete antioxidants. The purpose of this study was to determine whether cigarette smoking and other lifestyle choices may affect serum lipid peroxide concentrations, serum antioxidant concentrations such as tocopherol and vitamin C, and serum antioxidant enzyme activity such as glutathione peroxidase and superoxide dismutase. Methods: Dietary intakes were assessed by 24-hour recall and survey questionnaires from 48 male non-smokers and 52 male smokers. Ovemight fasting blood was collected and measured for individual antioxidant status. Results: The daily vitamin C intakes of smokers tended to be lower than those of non-smokers, and the intakes of both groups were under the Recommended Daily Allowance (RDA). Vitamin E intake was sufficient for smokers and non-smokers. Serum lipid peroxide concentrations were no difference among all subjects. The serum a-tocopherol concentrations of all subjects were in a normal range, and they were highest in mild smokers (p<0.05). Mean serum vitamin C levels were lowest in heavy smokers (p<0.05). The activities of serum glutathione peroxidase and superoxide dismutase were not significantly different in smokers and non-smokers. Conclusions: Smoking did not increase oxidative stress in heathy young men. However, it is desirable for heavy smokers to consume more vitamin C than the RDA since their serum vitamin C concentrations are relatively low.

한국의 제2형 당뇨병 환자에서 지속적 피하 인슐린 주입 치료가 산화적 스트레스에 미치는 영향

최수봉(Soo Bong Choi),최의광(Eui Kwang Choi),안승희(Seung Hee Ann),최미경(Mi Kyung Choi),박선민(Sun min Park) 대한내과학회 2000 대한내과학회지 Vol.58 No.5

N/A Background : It is known that hyperglycemia increase oxidative stress. Korean type 2 diabetic patients usually appear to be insulin deficient and insulin resistant. The blood glucose control can be normalized by the intensive insulin therapy. It has been reported that hyperinsulinemia have harmful effects on oxidative stress. The purpose of this study was to determine whether continuous subcutaneous insulin infusion (CSII) therapy by insulin pump affects the defense mechanism of oxidative stress in Korean type 2 diabetic patients. Methods : Fasting blood from eighty three subjects was collected prior to starting CSII therapy using portable insulin pump and after hospitalization for 2 weeks. Serum and red blood cell lipid peroxide concentrations were analyzed by Yagi's methods. Serum tocopherol and vitamin C concentrations were measured by HPLC. Red blood cell glutathione peroxidase, superoxide dismutase and catalase activities were also measured. Results : The mean age of subjects were 50.0±10.9 years, and they had diabetes for 8.4±5.8 years. Their average body mass index was 23.4+2.7 ㎏/m2. Their blood glucose levels were not controlled by oral hypoglycemic agents, diet and exercise treatment. Patients were divided into two categories according to blood glucose levels prior to CSII treatment. One category consists of patients with less than 11.1 mmol/L of average daily blood glucose levels prior to CSII treatment (the controlled group). The other category consists of patients with more than 11.1 mmol/L of average daily blood glucose levels (the uncontrolled group). Patients in the uncontrolled group had higher serum lipid peroxide levels than those in the controlled group before CSII therapy. After 2 weeks of CSII therapy, oxidative stress was not changed in controlled and uncontrolled groups. Serum insulin levels of all patients were increased after CSII treatment, but the levels before and after treatments were in the normal range, not hyperinsulinemic. Also, serum insulin levels did not correlate with serum lipid peroxide levels regardless of CSII treatment. Average serum vitamin C levels were remarkably increased after the therapy in all patients, but average serum total tocopherol levels was not altered. Also, activities of antioxidant enzymes such as glutathione peroxidase, superoxide dismutase, and catalase were not significantly changed. Conclusion : The blood glucose levels were normalized with the normal ranges of serum insulin levels after two weeks of CSII treatment. However, serum lipid peroxide levels were not changed even though serum vitamin C levels were increased. It is concluded that the oxidative stress of Korean type 2 diabetic patients are not changed after two weeks of CSII treatment, but there may be possibility the longer duation of CSII therapy may gradually improve oxidative stress.(Korean J Med 58:548-559, 2000)

『공공단체등 위탁선거에 관한 법률』의 문제점과 개선방안 -제1회 동시조합장선거 수사결과를 중심으로-

최수봉 ( Soo Bong Choi ) 법조협회 2015 法曹 Vol.64 No.11

On 11th of March 2015, the nationwide simultaneous election for cooperative presidents has been held for the first-time, in accordance with the Act of Election commissioned by the Public Organizations etc. enforced on first of August 2014. As a result of the first direct election for cooperative presidents since the system has been introduced, it has been evaluated that the cost for election has been reduc ed and the regulations were made more efficiently and the atmosphere for clean election has been created by catching the public``s eyes and ears. However, some problems that were unexpected have been found during the process of uniformly controlling part of the elections of cooperatives and public organizations which has various characteristics. And also, it was not enough to eliminate fraudulent elections controlled by money. Therefore, to give a guideline for the future of Act of Election commissioned by the Public Organizations etc., firstly, the election law for cooperatives and legislation cases were examined and the present status and differences of each elections monitored by the National Election Commission have been analyzed. Secondly, the regulation system for Act of Election commissioned by the Public Organizations etc. was inspected and through analyzing various cases of investigations and all sorts of election statistics, it was found that the present rules of punishment for disqualified cooperative members and bribery were insufficient. To improve this problem, methods to confirm fair and objective voters and to intensify qualification for candidates were reviewed. Additionally, methods were suggested to secure the effectiveness of the regulations by modifying rules of punishment, and to help advance and improve the fairness of the election system related to regulations of electioneering. After all, Act of Election commissioned by the Public Organizations etc. has to be modified systematically through further case studies to eventually achieve a culture of a clean election.

90% pancreatectomized rats 에서 w-6 다중불포화지방과 비타민 E 섭취가 인슐린 저항성에 미치는 영향

최수봉(Soo Bong Choi),박선민(Sun Min Park) 대한내과학회 1999 대한내과학회지 Vol.56 No.4

N/A High intake of -6 polyunsaturated fat (PUFA) can increase oxidative stress, which may in turn increase insulin resistance and be the cause of metabolic syndrome X (diabetes mellitus). One of the ways to reduce oxidative stress is through the consumption of antioxidants, such as vitamin E. However, it is controversial whether the consumption of vitamin E alleviates insulin resistance. The purpose of this study was to determine whether the excessive intake of -6 polyunsaturated fat and vitamin E influences the whole body glucose disposal rate (GDR), glycogen deposits in the liver and muscles, and the triglyceride content of muscles in 90% pancreatectomized rats. Methods : Mildly diabetic rats were produced by removing 90% of the pancreas from Sprague Dawley rats aged 8 weeks. One week after surgery, the blood glucose levels of rats were more than 9.4 mmol/L, according to which the rats are considered to be diabetic. Two factors were examined in the 90% pancreatectomized rats: polyunsaturated fat levels with 40% and 10% of total energy intakes, and vitamin E contents with 300 IU and 30 IU per kg of diet mixture. Four different diets were given for 8 weeks. After 7 weeks of diet consumption indwelling catheters were inserted in the carotid artery and jugular vein of all rats so that the GDR could be measured while the rats were awake and in a relaxed state. Results : Daily calorie and PUFA fat intake levels were higher in the high PUFA groups of diabetic rats. Daily vitamin E intake of the high vitamin E groups was ten times higher than that of the low vitamin E groups (p<0.0001). The GDR was lower in high PUFA groups, and the high intake of vitamin E tended to decrease the GDR in diabetic rats. The GDR of DHPHE was significantly lower than that of DLPLE (p<0.05). Sham operated rats (the control group) had a higher GDR by 44% than diabetic rats. Vitamin E deposits in the liver in DHPHE were higher in DLPLE (p<0.05). Glycogen deposits in the liver of diabetic rats were significantly higher in DLPLE than DHPLE. Muscle glycogen content showed a similar tendency to liver glycogen content in different diet groups of diabetic rats. Triglyceride deposits in muscles did not differ according to dietary fat and vitamin E contents in diabetic rats. Conclusions : High intake of -6 PUFA increased insulin resistance, and high vitamin E intake did not alleviate insulin resistance. Thus, increased oxidative stress may be an independent factor for increased insulin resistance in high intake of -6 PUFA. It is necessary to consume a minimum amount of -6 PUFA as well as the Recommended Daily Allowance of vitamin E.

2. 당뇨병 환자의 치료를 위한 인공췌장기의 자동 회로 설계

최현(CHOI HYUN),최수봉(CHOI SOO BONG),이근철(LEE KEUN CHUL) 대한전자공학회 1981 대한전자공학회 학술대회 Vol.1981 No.8

1차원 금속나노구조체에서 표면플라스몬을 이용한 광학장 제어

변지수(Clare C. Byeon),최수봉(Soo Bong Choi),박두재(DooJae Park),정문석(Mun Seok Jeong),김대식(D. S. Kim) 대한기계학회 2010 대한기계학회 춘추학술대회 Vol.2010 No.5

Plasmonics, utilizing the surface plasmons in metal nanostructure, is one of the key future technologies for developing ultra small and ultrafast devices. We have demonstrated the generation of surface plasmon polariton (SPP) in 1-dimensional periodic metal nano slit arrays and the directional control of SPP waves propagating through an asymmetric plasmonic Bragg resonator by femtosecond coherent control using femtosecond optical excitation.

백서에서 식이내 열량 영양소의 배분이 인슐린 분비능과 인슐린 저항성에 미치는 영향

박선민(Park Sunmin),최미경(Choi Mi Kyung),안승희(Ahn Seung Hee),김영희(Kim Young Hee),박춘희(Park Chun Hee),최수봉(Choi Soo Bong) 韓國營養學會 2001 Journal of Nutrition and Health Vol.34 No.5

홍삼추출물에 의한 유전독성 감소 효과(Ⅰ) - 배양 NIH3T3 세포에서 자외선에 의한 유전독성의 감소에 미치는 홍삼추출물 처리효과

김완주 ( Wan Ju Kim ),유병수 ( Byung Soo Ryu ),전병훈 ( Byung Hoon Chun ),이정섭 ( Jung Sup Lee ),박상대 ( Sang Dai Park ),김인호 ( In Ho Kim ),최수봉 ( Soo Bong Choi ),박종군 ( Jong Kun Park ) 대한화장품학회 1998 대한화장품학회지 Vol.24 No.1

자외선에 의한 유전독성의 감소에 미치는 홍삼추출물의 영향을 배양 NIH3T3 세포계에서 분석하였다. 자외선을 조사한 후 정상 배지에서 배양한 시간간격에 따라 세포의 생존률은 증가하였는데 홍삼추출물이 함유된 배지에서 배양한 경우는 약 15%정도 증가한 생존률을 보였다. 자외선을 조사한 후 감소된 DNA복제가 정상배지 배양시간에 따라 증가하는 정도도 홍삼추출물을 후처리할 경우 현저한 중가를 보였다. 자외선 상해를 회복하기 위한 절제회복능은 홍삼추출물을 처리할 경우 유의미한 증가를 보였다. 이러한 절제회복과정중 효소에 의한 절제단계가 홍삼추출물 처리에 의해 활성화됨을 단사절단 분석을 통하여 규명하였다. 이상의 결과는 홍삼추출물이 자외선 상해의 절제 회복에 유의미한 증가를 보이며 따라서 유전독성을 감소시키는 항노화제로써 사용할 수 있음을 시사한다. We have studied the effects of red ginseng root extract on the decrease of UV-induced genotoxicity in cultured NIH3T3 cells. The increase in survival and the recovery from DNA synthesis inhibition in UV-irradiated cells as a funtion of normal medium incubation time was potentiated by the presence of the ginseng extract. The extract also increased the UV-induced excision repair as determined by unscheduled DNA synthesis. The amount of UV-induced DNA single strand breaks that are accumulated by polymerase inhibitors was signifcantly increased by the presence of the extract. These results suggest that the red ginseng extract activates the incision/excision step of UV-induced repair and could be used as a reagent for protecting UV-induced genotoxicity and cytotoxicity.

消渴 치료 處方 加減이 3T3-L1 Adipocytes에서 인슐린 유사성과 인슐린 민감성에 미치는 영향

박선민(Sun Min Park),최미경(Mi Kyung Choi),전동화(Dong Wha Jun),최수봉(Soo Bong Choi),박성규(Seong Kyu Park),이미영(Mi Young Lee),김호경(Ho Kyoung Kim),황영희(Young Hee Hwang),고병섭(Byoung-Seob Ko) 한의병리학회 2004 동의생리병리학회지 Vol.18 No.2