마산만에서 입자수치실험에 의한 해파리 분포연구

최민호,유태관,김동선,Choi, Min-Ho,Ryu, Tai-Gwan,Kim, Dong-Sun 해양환경안전학회 2016 해양환경안전학회지 Vol.22 No.4

마산만에서 수치모델을 이용하여 해파리의 시 공간적 분포를 파악하였다. 먼저 3차원 해수유동모델(POM)을 이용하여 조석, 바람, 수온 염분의 효과를 고려한 잔차류를 계산하였다. 마산만의 마산항, 중공업단지, 가포신항에서 발생한 해파리의 거동을 파악하기 위하여 잔차류에 의한 입자추적자 모델을 이용하였다. 해파리의 분포는 마창대교 북쪽해역에서 최대인 2,533 개체수가 나타났다. 이와 같은 현상은 잔차류의 다방향으로 혼재하는 흐름과 지형적인 영향으로 판단된다. 해파리의 이동에 영향을 미치는 잔차류가 우세한 해역에서 외력조건에 따른 해파리의 집적도를 알아보기 위해서 조석잔차류, 취송류, 밀도류의 유형별 수치실험을 하였다. 특히 마산만의 마창대교와 마산만 입구인 모도해역에서는 취송류(바람에 의한 효과)가 해파리의 집적에 탁월한 것을 확인하였다. The spatio-temporal distribution of jellyfish in Masan Bay was investigated in this study using a numerical model. First, a three-dimensional hydrodynamic model (POM) was constructed,taking into account residual flows, tides, temperature, salinity, and wind effects. A particle tracking model based on residual flow was then used to investigate the jellyfish present in Masan Port, referred to as the Heavy Industry and Gapo New Port in Masan. Jellyfish distribution was concentrated with maximum (2,533 individual) in the North Sea near Machang Bridge. Itcan be concluded that this concentration was due to multi-directional residual flows and topography effects. Residual flow currents are a dominant factor in understanding the aggregation of jellyfish, and this study used a numerical simulation of tide-induced residual currents, wind-driven currents and density currents in distinct cases to thoroughly address the topic. As a result, wind-driven currents (effect of the wind) was found to be superior to other components as an influence on the distribution of jellyfish near Machang Bridge and Modo in Masan Bay.

음식물 쓰레기의 호기성분해를 위한 고온균의 분리 및 생육 특성

최민호,조성은,유정목,정윤진,박연희,Choi, Min Ho,Cho, Sung Eun,Yoo, Jung Mok,Chung, Yoon Jin,Park, Yun Hee 유기성자원학회 1995 유기물자원화 Vol.3 No.1

음식물 쓰레기 퇴비화 장치에 사용하기 위한 미생물제제 개발을 목적으로 토양, 퇴비 등으로부터 고온균을 분리하여 그 생육 특성을 조사하였다. 분리한 81주 중에서 음식물 쓰레기를 분해시키는 전분 분해효소, 단백 분해효소, 지방 분해효소 및 섬유소 분해효소의 활성이 높은 20주를 분리하여 형태 및 생리적 특성을 조사한 결과 모두 그람 양성의 간균으로 카탈라제를 가지고 있었으며 17주는 포자 형성균으로 밝혀졌다. 이 균주들은 대부분 pH 5에서 pH 10까지 생육할 수 있었으며 8% NaCl이 함유된 배지에서도 성장하였다. 또한 각 효소의 생산 균주 그룹별로 생육에 미치는 영향을 조사한 결과 통기에 의해 생육이 크게 증가하였으며, $50^{\circ}C$ 이상 온도가 올라갈수록 생육이 저하되었다. For development of microbial additives applicable to in-vessel composting system of food waste, thermophilic bacteria which showed amylase, protease, lipase and cellulase activity were isolated from soil, compost and food waste. Among 81 isolates, the growth characteristic of 20 strains with high enzyme activity were examined. All strains are Gram positive rod with catalase activity and 17 strains are spore formers. At $50^{\circ}C$, most of the strains were able to grow from pH 5 to pH 10 and in presence of 8% of NaCl. In trypticase soy broth, the growth of these strains was greatly increased by aeration, but decreased at elevated temperature above $50^{\circ}C$.

Nested PCR를 이용한 Streptococcus mutans의 검출

최민호,유소영,임채광,강동완,국중기,Choi, Min-Ho,Yoo, So-Young,Lim, Chae-Kwang,Kang, Dong-Wan,Kook, Joong-Ki 한국미생물학회 2006 미생물학회지 Vol.42 No.1

치아우식중의 원인균 중 하나인 Streptococcus mutans를 종 수준에서 동정할 수 있는 중합효소연쇄반응 프라이머를 개발하기 위하여 본 연구를 시행하였다. 표준균주인 S. mutans ATCC $25175^T$및 본 연구에서 이용된 S. mutans 균주들의 16S rRNA 유전자 핵산염기서열을 바탕으로 S. mutans 종-특이 중합효소연쇄반응 프라이머(ChDC-SmF2와 ChDC-SmR2)를 설계 및 제작하였다. 프라이머의 특이도는 S. mutans 11균주와 구강 내 존재하는 12세 균 종(22균주)을 대상으로 실시하였다. 프라이머의 민감도는표준균주인 S. mutans ATCC $25175^T$의 유전체 DNA를 추출하여 실시하였다. 프라이머의 특이도 실험결과 10균주의 S. mutans 유전체 DNA에서만 종-특이 중합효소 연쇄반응 산물이 증폭되었고, 다른세균종의 유전체 DNA에서는 증폭되지 않았다. 또한 감수성 실험 결과 본 연구에서 사용된 프라이머는 direct PCR에 의해 S. mutans ATCC $25175T\^T$ 유전체 DNA 100 pg까지 검출 할 수 있었다. 또한 27F와 1492R 프라이머로 16S rDNA를 먼저 증폭한 다음, 이 증폭물 10배 회식한 것을 표적으로 해서 nested PCR을 시행한 결과 S. mutans ATCC $25175^T$ 유전체 DNA를 2 fg까지 검출할 수 있었다. 이상의 결과를 종합할 때, ChDC-SmF2와 ChDC-SmR2 프라이머들은 S. mutans 균주 유전체 DNA에 대한 높은 민감도를 가지고 있으며, 종-특이적으로 동정 및 검출하는 데 이용될 수 있을 것으로 생각된다. This study was undertaken to develop PCR primers for the identification and detection of Streptococcus mutans (by)using species-specific forward and universal reverse primers. These primers targeted the variable regions of the 16S ribosomal RNA coding gene (rDNA). The primer specificity was tested against 11S. mutans strains and 10 different species (22 strains) of oral bacteria. The primer sensitivity was determined by testing serial dilutions of the purified genomic DNA of S. mutans ATCC $25175^T$. The data showed that species-specific amplicons were obtained from all the S. mutans strains tested, which was not observed in the other species. The direct and nested PCR could detect as little as 2 pg and 2 fg of the chromosomal DNA from S. mutans ATCC $25175^T$, respectively. This shows that the PCR primers are highly sensitive and applicable to the detection and identification of S. mutans.

구리 CMP 공정변수 최적화를 위한 실험계획법(DOE) 연구

최민호,김남훈,김상용,장의구,Choi, Min-Ho,Kim, Nam-Hoon,Kim, Sang-Yong,Chang, Eui-Goo 한국전기전자재료학회 2005 전기전자재료학회논문지 Vol.18 No.1

Chemical mechanical polishing (CMP) has been widely accepted for the global planarization of multi-layer structures in semiconductor manufacturing. Copper has been the candidate metallization material for ultra-large scale integrated circuits (ULSIs), owing to its excellent electro-migration resistance and low electrical resistance. However, it still has various problems in copper CMP process. Thus, it is important to understand the effect of the process variables such as turntable speed, head speed, down force and back pressure are very important parameters that must be carefully formulated in order to achieve desired the removal rates and non-uniformity. Using a design of experiment (DOE) approach, this study was performed investigating the main effect of the variables and the interaction between the various parameters during CMP. A better understanding of the interaction behavior between the various parameters and the effect on removal rate, non-uniformity and ETC (edge to center) is achieved by using the statistical analysis techniques. In the experimental tests, the optimum parameters which were derived from the statistical analysis could be found for higher removal rate and lower non-uniformity through the above DOE results.

농촌지도사업(農村指導事業)의 투자효과(投資效果) 변화(變化)의 추이(推移);지도사업(指導事業)의 구조변화(構造變化)에 대응(對應)하여

최민호,최영찬,Choi, Min-Ho,Choe, Young-Chan 한국농촌지도학회 1995 농촌지도와 개발 Vol.2 No.1

This study examines the socioeconomic returns to agricultural research and extension services in Korea, using multivariate time series technique and Akino-Hyami formula. Results find that the socioeconomic returns are quite competitive in case of agricultural research with 44.82% internal rate of return and very high in case of extension services with 207.82% internal rate of return. Agricultural production responds to the agricultural research shock about four years after the shock. The magnitudes of the impacts increase until a peak is reached nine years after the initial expenditures and the impacts declines to a zero level after about twenty years. This lag lengths are consistent to the usual literature on research, which finds lags of seven to thirty years. Agricultural production responds to an agricultural extension shock immediately and declines to a zero level after about four years. Thus, the lag lengths are much shorter than those by research shock.

다수 사용자 채널을 위한 효율적인 이산 비트 할당 방법

최민호,송상섭,Choi, Min-Ho,Song, Sang-Seob 한국통신학회 2004 韓國通信學會論文誌 Vol.29 No.9A

본 논문에서는 VDSL 시스템의 성능을 향상하기 위하여 , 각 사용자의 송신 전력이 제한된 경우, 채널 정보를 이용해 1 천송 비트율을 최대화 하는 이산 비트 할당법을 제안하였다. 누화잡음 전력이 클 경우 어느 사용자의 전력할당은 같은 케이블을 사용하는 다른 사용자에 마치는 누화전력을 변화시킨다. 이 경우 모든 사용자의 전력할당을 같이 고려하여야 성능을 향상 시킬 수 있다. 제안한 방법의 성능을 iterative water-filling 의 성능과 비교하여 더 향상된 결과를 얻었다. In this paper we propose a discrete bit-loading algorithm that maximizes the transmit bit rate using the channel information. to optimize the performance of the very high-speed digital subscriber line(VDSL) system under the constraint of a maximum transmit power for each user. When the power level of crosstalk is high, the power allocation of a user changes the crosstalk experienced by the other users in the same binder. In this case, the performance of DSL modems can be improved by jointly considering the bit and power allocation of all users Simulation results shows that the proposed method improves the performance compared With that of iterative water-filling method.

페놀 - 포름알데히드 레졸 수지의 경화 특성에 관한 연구

최민호,정인재 ( Min Ho Choi,In Jae Chung ) 한국화학공학회 1996 화학공학의이론과응용 Vol.2 No.2

소아청소년 기생충 감염의 최신 경향 및 치료

최민호,Choi, Min-Ho 대한소아소화기영양학회 2008 Pediatric gastroenterology, hepatology & nutrition Vol.11 No.suppl1

Enterobiasis and head lice infestations, contact-borne parasitic diseases, are the most prevalent parasitic infections of children in Korea, and they are transmitted by close contact with infected persons. They are not of major concern in public health, therefore their importance in children's health care are overlooked in Korea. Egg positive rates of Enterobius vermicularis have been reported to be 10~20% among children in kindergartens and primary schools. Enterobiasis is diagnosed by scotch tape anal swab, but repeated examination is necessary for accurate diagnosis. Albendazole or mebendazole are extremely effective for treatment, however, reinfections usually occur if treatment is not repeated at least three times at a 3-week-interval, targeting the whole family members including the patients. Environmental sanitation and health education are also necessary to guarantee the successful treatment of enterobiasis. Head lice infestation is still an indication of public health status. Children of 3 to 12 years old and their families have infestations more frequently, and girls have more than boys. Diagnosis can be made by identification of live lice or nits on the head. Pediculicides are effective treatment of infestations, however they do not kill nits completely, requiring second treatment. Recently wet combing is preferred as an alternative by parents in England because of possible toxicity of drugs and resistance of lice. It is impossible to completely prevent enterobiasis and/or head lice infestations. Therefore, mass screening and prevention are required, and, in local clinics, a thorough evaluation of the symptoms and past history, and appropriate laboratory tests are necessary.

95년도 지방화시대 농촌지도사업의 발전방향 학술발표회 결과 종합

최민호(MIn Ho Choi) 한국농촌지도학회 1995 농촌지도와 개발 Vol.2 No.2

N/A

형질전환 Chinese Hamster Ovary 세포에서 Albumin-erythropoietin의 생산시 Silkworm Gland Hydrolysate의 효과

최민호(Min-Ho Choi),차현명(Hyun-Myoung Cha),김선미(Sun-Mi Kim),최용수(Yong-Soo Choi),김동일(Dong-Il Kim) 한국생물공학회 2013 KSBB Journal Vol.28 No.2

To date, various strategies have been studied to increase specific productivity in Chinese hamster ovary (CHO) cell cultures. Also, albumin-fusion platform is being applied to other important bioactive peptides with short half-lives. Here, we investigated the effects of silkworm gland hydrolysate (SGH) on the production of albumin-erythropoietin (Alb-EPO) in transgenic CHO cells. The viable cell density of CHO cells was increased by 13% in the medium containing 1 mg/mL SGH higher than in the control medium without SGH. In addition, the production of Alb-EPO was also 1.26-fold enhanced by reducing the early apoptosis of CHO cells. In conclusion, SGH could be used as a useful supplement for the enhancement of recombinant protein production.