돼지 Lecithin : Cholesterol Acyltransferase 의 화학적 성질

최명숙,박용복 ( Myung Sook Choi,Yong Bok Park ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.1

Amino acid residues of lecithin: cholesterol acyltransferase from hog plasma were modified with various chemicals in order to identify the functionally important amino acid residues of the enzyme. At least one sulfhydryl group was found to be essential for the catalytic activity and all four of the sulfhydryl groups may be involved in the self-association process at high enzyme concentrations in vitro. During the self-association process, the sulfhydryl groups may participate in the intermolecular disulfide bond formation. More than one half of tryptophan residues of the enzyme was exposed to the aqueous environment under non-denaturing conditions. Fluorescence quenching studies showed that apolipopotein-AI in the high-density lipoproteins is one of the major contributors in the substrate binding of the enzyme. This interaction seems to be mediated mainly by the hydrophobic interaction between the substrate and the enzyme. The enzyme activity was sensitively modified by thiol, seryl and histidyl modifiers, indicating an importance of those amino acid residues in the function of the enzyme.

한의약학(韓醫藥學)의 서지학적(書誌學的) 연구(II)-[신상한론(新傷寒論)] - 노장사상(老莊思想)에 의한 한의약학의 과학적 통합이론 -

최명숙,임동술,이숙연,Choi, Myung-Sook,Yim, Dong-Sool,Lee, Sook-Yeon 대한약학회 2007 약학회지 Vol.51 No.1

Traditional Medicine (TM) is called as philosophical medicine in Korea. An unified theory named as Sihnsang-hanron (SSHR) was hypothesized through studies of scientific analyses on various theories of TM. SSHR has extracted seven concepts which are six common ones from the great three books (三大原典) and the Logic of Laotzu & Chuangtzu (老莊思想). Six common concepts are the affecting by cold (傷寒), qi (氣), cold or heat (寒熱), exterior & interior of body (表裏), deficiency or excessiveness (虛實), and yin & yang (陰陽). We have tried to apply these seven concepts to Physics and Life Science. The affecting by cold means anti-sunlight and the origin of all diseases. The difference between TM and modern medical science would be in diagnostic methods as well as their theoretical analyses for various diseases. The modern science follows Haeckel's positive dialectics applied by the biological monism, and oriental one(SSHR) does Yin-Yang monism from the studies of Logic of Laotzu (老子) & Chuangtzu (莊子). SSHR would make the theory of exterior & interior of body (表裏論) and six channels (六經) develope scientifically as a diagnostic technique of disease. This theory is an excellent one that can't be found out in modern medical science, and so it should be developed as a scientific theory by using modern mechanic instrument. Chuangtzu asserted that ai was the basic substance of the universe. It is hypothesized that qi (氣) is like small particles -higgs, with dynamic power in modern Physics. We consider cautiously qi could be calculated by mathematics through higgs' bosons in near future.

Effects of Human Breast Milk and Hyperlipidemic Serum on the Cholesterol Metabolism in Organ Culture of Fetal Aorta

최명숙,박용복,Choi, Myung-Sook,Park, Yong-Bok 생화학분자생물학회 1989 한국생화학회지 Vol.22 No.2

출생 때에 토끼는 성숙한 토끼보다 비교적 높은 혈중 콜레스테롤을 포함하고 있으며 그 농도는 수유에 따라 증가하여 수유 2주일에 최대치를 보이다가 수유기간이 끝나는 4주째에는 정상농도로 돌아가게 된다. 사람의 모유 또는 와타나베토끼(유전적으로 혈중 콜레스테롤이 비정상적으로 높은 토끼)의 혈청으로부터 분리한 저밀도 리포단백질을 배지에 포함시켜 출생전 토끼의 대동맥을 조직 배양할 경우 대동액내에 포함되어 있는 콜레스테롤 양의 변화를 관찰하였다. 사람의 모유를 함유한 배지에 먼저 배양한 후 와타나베토끼의 저밀도 리포단백질을 함유한 배지로 옮겨 배양했을 때 출생전 토끼 대동맥내 콜레스테롤 농도는 사람의 모유에서만 같은 기간동안 배양한 대동맥에서와 비슷했다. 그러나 사람의 모유에서 먼저 배양하지 않고 와타나베토끼의 저밀도 리포단백질을 함유한 배지에서만 배양한 대동맥에서는 훨씬 높은 콜레스테롤 농도를 보여주었다. 이 결과로 미루어 보아 사람의 모유가 대동액내의 콜레스테롤 축척을 막아주는 역할을 한다는 사실을 추측할 수 있다. Milk was tested whether it plays roles as a cholesterol-lowering agent in suckling rabbits since a serum cholesterol content of newborn rabbit was increased till second week then gradually decreased during lactation. Cholesterol content of fetal rabbit aorta was compared after the aorta was cultured in the media containing human breast milk and WHHL (Watanabe heritable hyperlipidemic) rabbit LDL. In precultured aorta in the medium containing milk prior to culture with 5% hyperlipidemic LDL media, the aortic cholesterol content was similar to that of the aorta cultured in the human breast milk only. However, the cholesterol content of the aorta cultured in 5% hyperlipidemic LDL media without preculture in the human breast milk was significantly higher than that of the precultured aorta in the human milk. This suggests that milk led the fetal aorta resistent to cholesterol-elevating effect by an unknown mechanism.

Chemical Modifications of Lecithin: Cholesterol Acyltransferase from Hog Plasma

최명숙,박용복,Choi, Myung-Sook,Park, Yong-Bok Korean Society for Biochemistry and Molecular Biol 1991 한국생화학회지 Vol.24 No.1

Amino acid residues of lecithin:cholesterol acyltransferase form hog plasma were modified with various chemicals in order to identify the functionally important amino amino acid residues of the enzyme. At least one sulfhydryl group was found to be essential for the catalytic activity and all four of the sulfhydryl groups may be involved in the self-association process at high enzyme concentration in vitro. During the self-association process, the sulfhydryl groups may participate in the intermolecular disulfide bond formation. More than one half of tryptophan residues of the enzyme was exposed to the aqueous environment under non-denaturing conditions. Fluorescence quenching studies showed that apolipoprotein-AI in the high-density lipoproteins is one of the major contributors in the substrate binding of the enzyme. This interaction seems to be mediated mainly by the hydrophodic interaction between the substrate and the enzyme. The enzyme activity was sensitively modified by thiol, seryl and histidyl modifiers, indication an importance of those amino acid residues in the function of the enzyme. 돼지혈장으로부터 분리한 lecithin : cholesterol acyltransferase의 아미노산 잔기들을 여러 가지 화학물질로 변환시킴으로서 이 효소의 촉매기능에 중요한 역할을 담당하는 아미노산 잔기들을 규명하려 하였다. 최소한 한 개의 thiol기가 이 효소의 촉매반응에 필요하며 높은 효소농도에서는 네 개 모두의 thiol기가 분자간 disulfide 결합을 형성함으로서 효소분자간의 결합에 관여할 것으로 추정된다. 전체 tryptophan 잔기의 절반 이상이 효소분자의 표면에 노출되어 있으며 이러한 소수성 아미노산 잔기들이 이 효소의 기질인 high-density lipoprotein 표면의 apolipoprotein-AI과 소수성 결합에 관여함으로서 효소-기질 결합에 참여할 것으로 추정된다. 이 효소의 역가는 serine, thiol 그리고 histidine 잔기를 변환하는 화학물질에 의해 민감하게 저해됨으로서 이러한 아미노산 잔기들이 이 효소의 촉매반응에 관여함을 제시해주고 있다.

한의약학의 서지학적(書誌學的) 연구(I) -상한론(傷寒論)을 중심으로-

최명숙,임동술,이숙연,Choi, Myung-Sook,Yim, Dong-Sool,Lee, Sook-Yeon 대한약학회 2006 약학회지 Vol.50 No.4

Three important books in the oriental medicine are Huangjenaekyung (medical book), Sanghanron (pharmacological book) and Shinnongbonchokyung (herbal book). Chang, joong-kyung who is the writer of Sanghanron would be recommended as a saint physician and his book, Sanghanron is granted for a best book in this field by Chinese people. Also, Japanese physicians would estimate Sanghanron as the most magnificent book ever written by a human being. Sanghanron contains a lot of confirmations (證) of diseases, their progressing courses and exact therapeutic ways. Therefore modern physicians and pharmacists who are interested in the oriental medicine might study and practice more scientific approach of therapy for patients. The aim of this bibliographical study on Sanghanron is to explain systematically theories of this book and harmonize them with the modern medicinal study: In this study we have acquired three main results, the first is that we could get the coincidence between western & oriental way on the five therapeutic principles-sweating, vomiting, harmonizing, excreting and bleeding : the second, notice the exclusion of the Five Element Theory(the fundamental one in oriental medicine) and the third, found out the possibility of the immunological and neuroscientific explanation for pharmacological application to the oriental medicine.

사람의 모유가 대동맥내의 콜레스테롤대사에 미치는 영향

최명숙,박용복 ( Myung Sook Choi,Yong Bok Park ) 생화학분자생물학회 1989 BMB Reports Vol.22 No.2

Milk was tested whether it plays roles as a cholesterol-lowering agent in suckling rabbits since a serum cholesterol content of newborn rabbit was increased till second week then gradually decreased during lactation. Cholesterol content of fetal rabbit aorta was compared after the aorta was cultured in the media containing human breast milk and WHHL (Watanabe heritable hyperlipidemic) rabbit LDL. In precultured aorta in the medium containing milk prior to culture with 5% hyperlipidemic LDL media, the aortic cholesterol content was similar to that of the aorta cultured in the human breast milk only. However, the cholesterol content of the aorta cultured in 5% hyperlipidemic LDL media without preculture in the human breast milk was significantly higher than that of the precultured aorta in the human milk. This suggests that milk led the fetal aorta resistent to cholesterol-elevating effect by an unknown mechanism.

Effects of Human Breast Milk on Lipid Metabolism in Organ Culture of Fetal Rabbit Aorta

최명숙,박용복 ( Myung Sook Choi and Yong Bok Park ) 한국영양학회 1991 정기총회 및 심포지움 Vol.- No.26

중국 ‘경가풍(京歌風)’ 대중음악 고찰 — 리위강(李玉剛)을 중심으로

최명숙(Choi, Myung-Sook) 중국문화연구학회 2021 중국문화연구 Vol.0 No.51

The wave of contemporary popular songs swept in, the traditional genre of Beijing Opera and modern music combined to create freshness and interest in the public with unique music styles. It is noteworthy that traditional performing arts in China are of great value in that they preserve traditional Chinese culture and are focusing on popularization and industrialization as cultural products. “Jingge” in the text refers to popular music created using a new technique that combines contemporary music techniques with theatrical tunes, i.e., the cast, singing, and musical instruments of the Kyong drama. Singer Li Yugang, who was praised as the modern Mei Lanfang, his production of Namdan were sophisticated and delicate, and the expression technique of traditional drama harmonized in his music. He transformed the art form of the traditional Beijing Opera into a new genre of popular music, crossing between men and women on the stage. He was loved by the public for every step of his journey with such a deep taste of Chinese culture, and are now heating up as the “Li Yugang phenomenon.” The sensation he created led to the popularization of “Jingge” and it has been deeply ingrained in the hearts of the public even now. In this study, we focus on the ‘Li Yugang’ phenomenon, which is created by the combination of Beijing Opera and modern music, and look at the creation of “Jingge” and its musical characteristics, as well as the “nandan” artistic reform of Li Yugang.

한국과 중국의 근대 대중가요 민족화 연구 - 신민요와 신민가의 비교를 중심으로

최명숙(Choi, Myung-Sook) 중국문화연구학회 2020 중국문화연구 Vol.0 No.47

From the 1920s to the 1940s, Korean and Chinese pop songs were in full bloom and enjoyed by the public. Recognizing that Korean and Chinese pop music has not been placed on the comparative list of studies together despite their historically close correlation, this study aims to reveal the development patterns of modern pop songs in Korea and China and explore new areas of research. The invasion of Western powers, which began at the end of the 19th century, opened the doors of China, and Korea also began to undergo drastic social changes due to Japanese aggression and colonial rule. In the process of spreading capitalist culture centered on Western Europe and Japan, Korean and Chinese pop music developed into diverse forms through fusion and harmony, not the dichotomy between traditional and foreign styles. In the 1930s, K-pop and Chinese pop developed with the popularity of ‘Shin Minyo’ and ‘Shin Min-ga’ based on their respectively traditional folk songs. In this text, we will look at the musical content of Korea’s “Shin Minyo” and China’s “Shin Min-ga,” which were widely sung in the 1930s and 40s and is an important part of pop music. At Japanese colonial period, 〈ours〉 exist in the two countries pop songs which fill with the public longing and desire, strive to succeed the trails of the traditional folk songs and attach meaning to look at nationalization of pop songs and Popularization and Cultural Phenomenon. In particular, the two genres are have in common with emerging in historical backdrop of the Japanese colonial period, so wo can say, it is a more precious tasks.

초등학교 코바늘뜨기 교수ㆍ학습 자료의 개발과 적용

최명숙(Choi Myung Sook),정지윤(Jung Ji Yoon),황연순(Hwang Yeon Soon) 한국실과교육학회 2005 한국실과교육학회지 Vol.18 No.4

The aim of crochet lace class in preferential learning of ‘skill embroidery and knitting’ unit is maximized academic achievement, made learning ability that own guide and developed creativity. Experience about crochet lace of children was lowest and most felt difficult. Therefore, this study wished to make teaching guidance with learning materials that children can think easily and learn easily crochet lace. Teaching-learning materials developed that can complete at class if is possible. Teaching-learning materials was hair pin, cup mat, muffler, bag, hat. Those were developed setting in elementary student’s ability. Teaching-learning guidance composed to complete living goods at 4unit class. Teaching learning guidance’s characteristics learn crochet lace in process that make living goods and can complete to two weeks by selection by ability. As a result, children’s academic achievement and creavity can be improved by learning materials and teaching-learning guidance.