식이지방의 종류가 백쥐 상피 세포의 Protein Kinase C 활성에 미치는 영향

최면,김종대,주진순 ( Myeon Choe,Jong Dai Kim,Jin Soon Ju ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.4

To determine the effect of dietary fats on Protein Kinase C(PKC) activity in mouse epidermal cells, female Balb/c mice (4 weeks of age) were placed on one of the 15% (w/w) of corn oil, sesame oil or beef tallow supplemented diets for at least 4 weeks. Protein kinase C was assayed by the modified procedure of Wise et al., and the activities among the groups were compared to that of corn oil group as the control. An apparent decrease of PKC activity in both cytosolic and membrane-associated fractions was observed from the animal fed sesame oil diet when compare with the animal fed corn oil diet. However, PKC activity of beef tallow fed group was increased slightly. In summary, dietary fats may contribute to mechanism of enhanced tumor promotion by changing PKC activity in mouse skin model.

식이지방의 종류가 백쥐 상피세포의 Protein Kinase C 활성에 미치는 영향

최면,김종대,주진순,Choe, Myeon,Kim, Jong-Dai,Ju, Jin-Soon 생화학분자생물학회 1991 한국생화학회지 Vol.24 No.4

식이지방의 종류가 Protein Kinase C(PKC) 활성에 미치는 영향을 고찰하기 위하여 Balb/c 백서를 3군으로 나누어 옥수수유, 우지, 참깨유를 식이지방원으로 15%(w/w)씩 4주간 투여한 후 상피세포를 분리하여 Wise 등의 방법으로 PKC 활성을 측정하였다. 참깨유로 사육된 백서의 상피세포를 옥수수유로 사육된 경우와 비교할 때, cytosolic 및 membrane-associated PKC 활성은 모두가 현저히 감소하는 것이 관찰되었으며 유의적은 아니지만 우지로 사육된 백서 상피세포는 반대의 경향을 보여 증가하는 경향을 고찰하였다. PKC 활성이 cell differentiation이나 proliferation 과정에 접지적 역할을 한다는 사실과 membrane-associated PKC가 발암물질인 TPA와 같은 phorbol esters의 수용체로서의 기능을 고려할 때 본 실험결과는 식이지방의 종류가 백쥐 상피 세포의 PKC활성을 변화시킴으로서 발암과정의 저해나 촉진을 가져올지도 모른다는 것을 암시하고자 한다. To determine the effect of dietary fats on Protein Kinase C(PKC) activity in mouse epidermal cells, female Balb/c mice (4 weeks of age) were placed on one of the 15% (w/w) of corn oil, sesame oil or beef tallow supplemented diets for at least 4 weeks. Protein kinase C was assayed by the modified procedure of Wise et al., and the activities among the groups were compared to that of corn oil group as the control. An apparent decrease of PKC activity in both cytosolic and membrane-associated fractions was observed from the animal fed sesame oil diet when compare with the animal fed corn oil diet. However, PKC activity of beef tallow fed group was increased slightly. In summary, dietary fats may contribute to mechanism of enhanced tumor promotion by changing PKC activity in mouse skin model.

Effect of Metal Ions on the Oxidation of Soybean Oil and Its Fried Noodle

최면,김태웅,이양희,Choe, Myen,Kim, Tae-Woong,Lee, Yang-Hee Korean Society of Food Science and Technology 1977 한국식품과학회지 Vol.9 No.2

본(本) 실험(實驗)은 대두유(大豆油)와 그 튀김면(麵)에, 한국(韓國) 상수도(上水道) 기준(基準) 또는 물리적(物理的)으로 정제(精製)한 지하수(地下水)에 함유가능(含有可能)한양(量)과 동일(同一)한 양(量)의 철(鐵) ion과 동(銅) ion을 각각(各各)에 첨가(添加)하여 그들에 의한 산패촉진정도(酸敗促進程度)를 측정(測定)하고, 아울러 일반적(一般的)으로 널리 쓰이고 있는 유지(油脂)의 황산화제(抗酸化劑)로서 BHA (butylated hydroxyanisole) 또는 citric acid가 이들 금속(金屬)에 의(依)한 산패촉진정도(酸敗促進程度)를 어느 정도 감소(減少)시키는 가를 비교(比較) 검토(檢討)하였다. 본(本) 실험(實驗)의 결과(結果), 대두유(大豆油)와 그 튀김면(麵)의 경우 모두, 철(鐵) ion 또는 동(銅) ion의 첨가(添加)는 이들 ion이 첨가(添加)되지 않은 경우와 비교(比較)할때 뚜렷한 산패촉진효과(酸敗促進?果)를 보였다. 특히, 동(銅) ion이 첨가(添加)된 경우에 있어서는 시간(時間)이 경과(經過)함에 따라 철(鐵)ion이 첨가(添加)된 경우보다 3배(倍) 내지 6배(倍)의 강(强)한 산패촉진효과(酸敗促進?果)를 보였다. 항산화제(抗酸化劑)인 BHA와 citric acid의 이들 금속(金屬)에 의(依)한 산패촉진(酸敗促進)에 대(對)한 저해작용도(沮害作用度)는 citric acid가 첨가(添加)된 경우가 BHA첨가(添加) 경우보다 컸으며, BHA가 첨가(添加)된 경우 그 효과(?果)는 극(極)히 미약(微弱)하였다. 또한 이들 항산화제(抗酸化劑)의 저해작용도(溫害作用度)는 동(銅) ion의 경우보다 철(鐵) ion의 경우에 더 효과적(效果的)인 것으로 보여진다. In the present study, the proxidant effect of ferrous and cupric chlorides which added to soybean oil and its fried noodle in the same concentration of the city water and the physically refined underground water were determined. As the inhibitor of metal proxidation, BHA and citric acid which are used widely in oil industry as antioxidants were compared. In both cases of soybean oil and its fried noodle, the addition of cupric chloride and ferrous chloride showed prominent proxidant effect. Especially, cupric chloride marked more proxidant effect than ferrous chloride by 3 to 6 times with the elapse of time. In the inhibition activity of metal proxidation, citric acid was more effective than BHA. The 1ppm of Cu +0.01% of citric acid treated soybean oil showed less proxidant effect than the control at the later stage. The inhibition activity of citric acid on $Fe^{++}$ in soybean oil was more effective than $Cu^{++}$ in soybean oil.

식이섬유가 무기질의 생체이용에 미치는 영향

최면,Choe, Myeon 한국식품위생안전성학회 1992 한국식품위생안전성학회지 Vol.7 No.4

Among the nutrients of biological importance, minerals are of particular interest in human nutrition because the range of adequate intake is so narrow. As the results of a series of interaction experiments between dietary fiber sources and minerals, there are many inconsistencies in the experimental data regarding the effect of dietary fibers on mineral bioavailability. the mechanism by which dietary fiber might influence mineral absorption is related to its physicochemical properties. These properties involve the ability of dietary fiber to (1) act as a weak cation exchanger, (2) decrease transit time, (3) dilute mineral concentration by increasing fecal bulk and (4) resist digestion in the large bowel. Regardless of the large number of human and animal studies available, a carful review of these publications dose not provide the answer as to whether the adverse effect of dietary fibers on mineral absorption is the fiber itself or some associated dietary factors( e.g. phytate, oxalate, ascorbate, citrate and protein, mineral-mineral interaction, etc) that are responsible for this action. As a result of the complexity of interaction that may take place between minerals. dietary fiber, and other component of food ; it becomes very difficult to blame fiber alone as a negative factor of mineral nutrition. We absolutely need more research with advanced tools rather than metabolic balance study.

식이섬유의 종류가 자연적 고혈압 유발 백서의 혈압 변화 및 Na 흡수에 미치는 영향

최면(Choe Myeon),태원찬(Tae Weon-Chan),김종대(Kim Jong-Dai) 韓國營養學會 1991 Journal of Nutrition and Health Vol.24 No.1

우리밀 Ethanol - Acetic Acid 추출물에 함유된 대식세포의 식작용 활성 증강 물질의 분리, 정제

최면(Myeon Choe),김선률(Sun-Lyul Kim),김종대(Jong-Dai Kim),이상영(Sang-Young Lee),김현숙(Hyun-Sook Kim) 한국식품영양과학회 2000 한국식품영양과학회지 Vol.29 No.2

본 연구에서는 우리밀로부터 대식세포 식작용을 활성화시키는 물질을 탐색하는 과정에서 고분밀의 EA 추출물이 가장 높은 식작용 활성 증강효과를 보임을 알았고, EA 추출물을 정성한 후 순수 분리 정제하여 대식세포 활성 증강효과를 나타내는 단일 물질을 찾고자 하였다. TLC방법을 이용하여 ASW와 고분밀의 EA 추출물을 정성한 결과 약 5개 정도의 band를 확인한 후, column chromatography를 이용하여 EA 추출물을 분획하였다. 1차 column chromatography만으로는 single fractionation이 어려워 분획물을 fraction A, B, C, D로 분류하여 식작용 활성 증강효과를 측정하였다. 대분류된 각 fraction의 식작용 활성 증강효과를 측정한 결과 고분밀의 fractionb가 대부분의 활성을 가진 분획으로 사료되었다. 2차 column chromatography를 통해 fraction b만을 분리하였으며, 구조결정 실험과 동물실험을 추가로 시행하여 fractionb의 효과를 입증할 것이다. To purify the macrophage phagocytic activity-enhancing component, ethanol-acetic acid (100 : 1, EA) extract of Korean wheat (Gobun wheat) and imported one (Australian Standard White, ASW) were fractionated with ethylacetate : methanol : H2O (65 : 25 : 4, v/v/v), and identified by TLC and column chro- matography. At least five fractions were separated from the EA extract of the wheats but amounts of fraction B, C and D were more in Gobun wheat than in ASW. The effects of all fractions on phagocytic activity were tested in macrophage J774 cells. Among the fractions, only fraction b of Gobun wheat showed significant increase of phagocytic activity against yeast.

지방질원으로서 어유가 백서 상피세포의 인지질 조성 및 Protein Kinase C 활성에 미치는 영향

최면(Myeon Choe) 韓國營養學會 1994 Journal of Nutrition and Health Vol.27 No.5

우리밀의 면역증강능 규명

최면(Myeon Choe),박재봉(Jae-Bong Park),김현숙(Hyun-Sook Kim) 한국식품영양과학회 2000 한국식품영양과학회지 Vol.29 No.2

본 연구는 우리밀 6종과 수입밀 2종의 PBS 및 ethanol : acetic acid (100 : 1, EA) 추출물을 제조하여 J774 macrophage를 대상으로 식작용 활성 증강 효과를 검증하였다. 각 용매 추출물의 대식 세포의 식작용 활성 증강효과를 비교했을 때 PBS보다는 EA 추출물의 식작용 활성이 크게 나타났고, 고분밀>알찬밀>그루밀>탑동밀≒수원267≒은파밀>대조군>ASW>DNS순이었다. 실험 조건의 최적화를 위하여 EA 추출물의 농도와 배양 시간에 따른 식작용 활성을 측정한 결과, 고분밀의 대식세포의 식작용 활성은 EA 추출물의 최종 농도 0.05 mg에서 가장 강하였고 안전한 조건에서 대식세포의 식작용 활성을 측정하기 위한 최적의 배양 시간은 2시간으로 나타났다. The purpose of this study was to identify excellent immune-enhancing substance from Korean wheats (Eunpa, Gueru, Alchan, Topdong, Suwon 267, Gobun) compared with imported ones (Australian standard white, ASW; Dark northern spring, DNS). Phagocytic activities of PBS (phosphate buffered saline, pH 7.4) and EA (ethanol-acetic acid) extracts from the wheats were determined using mouse macrophage J774 cell line. In order to set the optimal experimental condition up, the cultured cells were tested in varying experimental conditions. About two to five times higher phagocytic activity was shown in EA extract of Korean wheats compared to that of imported wheats. PBS extracts of wheats did not show increased phagocytic activity compared to control that did not add any extract. The EA extract of Gobun wheat showed the highest phagocytic activity. From the experiment we found that the optimal experimental condition was shown in two hours of reaction time and 0.05 mg amount of EA extract added to J774 cells.

메밀 보충급여가 백서의 혈당 및 혈압에 미치는 영향

최면(Myeon Choe),김종대(Jong-Dai Kim),박경숙(Kyung-Suk Park),오상용(Sang-Yong Oh),이상영(Sang-Young Lee) 한국식품영양과학회 1991 한국식품영양과학회지 Vol.20 No.4

메밀보충급여가 백서의 혈압 및 혈당에 미치는 효과를 검토 하고자 AIN-76 diet와 AIN-76 diet의 탄수화물원을 박피 메밀분으로 교체한 식이로 4주간 사육하여 성장율, 혈당, 인슐린 분비능, 혈압 등의 변화를 측정하고 고찰한 결과는 다음과 같다. 동일실험 기간동안 체중증가량 및 식이섭취량은 대조군과 메밀투여군 사이에 큰차이를 보이지 않았다. 메밀보충급여군은 혈당강하작용이 있음이 관찰 되었으며, 혈압은 유의성이 없으나 수치적으로 어느정도 감소시키는 경향을 보였다. 또한 메밀보충급여에 의해 공복 시 혈청 인슐린 수준은 낮았으며 혈당을 투여한 후 혈중의 당이 증가하면 분비되는 인슐린 양도 빠르게 증가하는 경향을 나타내었으나 유의성은 없었다. To investigate possible effects of buckwheat on blood glucose level and blood pressure, Sprague-Dawley rats were divided into two groups and fed either AIN-76 diet or modified AIN-76 diet with buckwheat flour for 4 weeks. At the end of experiment, determinations of blood pressure and blood glucose level(glucose tolerance test) were performed. In order to determine insulin levels in both fasted and glucose loaded serum, the animals in both groups were subdivided into two groups. Blood pressure was slightly decreased in the group fed buckwheat(-6mmHg) but not significantly. Glucose tolerance curve of the animal fed buckwheat flour showed clearly different pattern from that of the control group with decreasing tendency. Mobilization of serum insulin was 1.5 times faster in buckwheat group than in control group 1 hour after glucose loading. Further research is needed to find what substance(s) in buckwheat do this role.

식이 지방과 암

최면(Myeon Choe) 한국식품영양과학회 1991 한국식품영양과학회지 Vol.20 No.5

Evidence from recent studies in several laboratories indicates a relationship between type or level of fat in the diet and occurance of tumor at specific sites. The essential fatty acids in fat and degree of their unsaturation are important to determine the influence of a dietary fats on carcinogenesis. Alteration of dietary fat can also change carcinogenesis of cell in several tissues. Dietary fats appear to be important in both initiation and promotion stages of carcinogenesis. Several possible mechanisms have been investigated how dietary fat could affect to carcinogenesis at cellular level. One potential mechanism of dietary fat on carcinogenesis is through modulation of protein kinase C activity in the cell.