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기계적 자극을 이용한 인체 중간엽줄기세포로부터 유사-섬유모세포로의 분화 유도
최경민 ( Kyung Min Choi ),서영권 ( Young Kwon Seo ),윤희훈 ( Hee Hoon Yoon ),권순용 ( Soon Yong Kwon ),이화성 ( Hwa Sung Lee ),박용순 ( Yong Soon Park ),손영숙 ( Young Sook Son ),송계용 ( Kye Yong Song ),김영진 ( Young Jin Kim 한국조직공학과 재생의학회 2006 조직공학과 재생의학 Vol.3 No.4
Recently, in vitro reconstruction of anterior cruciate ligament(ACL) has been tried using tissue engineering technique, especially mesenchymal stem cell(MSC) is considered as a good cell source. In this study, we tried to find the effect of mechanical stimulus on the differentiation of bone marrow-derived MSC into ACL fibroblastlike cell using a cell training bioreactor imposing cyclic mechanical tension whose parameters were 120 min/day, 1 Hz, and 5% strain. After 2 weeks of culture, MSCs under cyclic tension showed more regularly oriented alignment and lower expression of CD73 and CD105 than control by microscopy and FACS analysis. In transcriptional level, type I collagen, type II collagen, fibronectin, elastin, α-smooth muscle actin mRNA expressions of MSCs under cyclic tension were higher than control and similar to native ACL. In conclusion, it is thought that mechanical cyclic tension induced the loss of stemness of MSC, therefore improved the differentiation into ACL fibroblast-like cell.
고온 호기법을 이용한 Poly Aluminum Chloride에 의해 응집된 조류의 분해특성
양재경(Jae-Kyung Yang),최경민(Kyung- Min Choi) 유기성자원학회 1999 유기물자원화 Vol.7 No.1
공기유입속도가 2171 . m-3• min-1 이었을 경우, 고온 · 호기조를 이용하여 PAC로 응집 , 탈수된 조류의 분해 특성을 관찰한 결과 다음과 같이 요약할 수 있다.10% 폐 식용유의 혼합조건이 본 연구에서 최적 조건으로 용도는 55"C 이상을 나타내었고, 수분은 55%를 일정하게 유지하였으며. COz의 농도는 최고 3.7%. 투입 탄소량의 92%가 CO2-C로 변환하였다. 적정한 폐식용유의 혼합은 미생물의 활동을 활발하게 하였지만 과도한 혼합은수분증발로건조상태로되었으며 오히려 분해 활성에 저해 영향을주었다. 처리가종료된 담체는고농도의 알루미늄이 축적되어 있으므로 별도의 처리가요구된다. The biodegradation of algae coagulated with poly aluminum chlorideCPAC) was investigated by using the thermophilic oxic process. The compositions of coagulated a1gae were 83.5% of water content. 24.6% of ash. 32% of organic carbon with in tota1 solid. respectively. In present study. food waste oil was used for the increment of ca10rie of mixtures in order to accelate the microbia1 activity. As a resu1t. the maximum temperature of mixtures was higher than 50"C when the mi피ng ratio of food oil was over 10%. However the temperature indicated the lower than 50"C when conditions of no mi잉ng with waste food 이1. and 5% of mixing ratio. Therefore. the optimum condition was 10% of the mixing ration at 217 l . m-3• min-1 of air supply rate. The conversion efficiency of carbon was highest as 92% at the optimum condition. And then water was eva1uated from imxture without accumulation at 10% of mixing ratio. The thermophilic oxic process well conducted that is good process for the treatment of waste a1gae without effluents however it has to consider the retreatment of accumu1ated aluminum in the reactor.
최경민,양재경,박응로,배진우,서용기,이성택,Choi, Kyung-Min,Yang, Jae-Kyung,Park, Eung-Roh,Bae, Jin-Woo,Seo, Yong-Ki,Lee, Sung-Taik 유기성자원학회 1997 유기물자원화 Vol.5 No.1
5-Aminolevulinic acid (ALA) 생합성의 $C_5$ 경로의 전구물질인 L-glutamic acid가 Rhodospirillum rubrum N-1 새포내에서 ALA 생산의 역할을 검토하였다. Lascelles의 기본배지에 L-glutamic acid와 levulinic acid (LA)를 각각 30, 20 mM 첨가배양으로써 균체외 ALA 생산성이 40배 증가(76 mg/l)하였다. 한편 $C_4$ 경로의 기질인 glycine과 succinic acid를 대수기 중기에 각각 60 mM 첨가함으로써, 균의 증식은 억제되었으나 균체외의 ALA는 52 mg/l에 달하였다. The role of L-glutamic acid, a precursor of $C_5$ ALA biosynthetic pathway, on the production of 5-aminolevulinic acid (ALA) has been described in cells of Rhodospirillum rubrum N-1. To the Lascelles basal medium the addition of both 30 mM L-glutamicacid and 20 mM levulinic acid (LA) provided to increase the extracellular ALA yield up to 40 fold (76 mg/l). By the addition of both 60 mM glycine and succinic acid, precursorsof $C_4$ ALA biosynthetic pathway, at middle log phase of cell growth ALA yield was increased 27 fold (52 mg/l) although the celt growth was inhibited to a certain extent.
알루미늄 옥사이드 절연층의 증착율이 유기박막 트랜지스터의 특성에 미치는 영향
최경민,형건우,김영관,조의식,권상직,Choi, Kyung-Min,Hyung, Gun-Woo,Kim, Young-Kwan,Choi, Eou-Sik,Kwon, Sang-Jik 한국전기전자재료학회 2009 전기전자재료학회논문지 Vol.22 No.12
In this study, we fabricated pentacene organic thin film trasistors(OTFTs) which used aluminum oxide as the gate insulator. Aluminum oxide for OTFTs was deposited on glass substrate with a different deposition rate by E-beam evaporation. In case of the deposition rate of $0.1\;{\AA}$, the fabricated aluminum oxide gate insulating OTFT showed a threshold voltage of -1.36 V, an on/off current ratio of $1.9{\times}10^3$ and field effect mobility $0.023\;cm^2/V_s$.
최경민,황승미,임지예,고은실,박종혁,문정혜,이민정,장지은,차정단,Choi, Kyung-Min,Hwang, Seung-Mi,Lim, Ji-Ye,Ko, Eun-Sil,Park, Jong-Hyuk,Moon, Jung-Hye,Lee, Min-Jung,Jang, Ji-Eun,Cha, Jeong-Dan 한국미생물·생명공학회 2015 한국미생물·생명공학회지 Vol.43 No.2
In this study, we investigated the effects of 40% ethanol extract of Red Ginseng (RGE) on the productions of inflammatory proteins in Antigen I/II (Ag I/II)-N, a recombinant protein isolated from Streptococcus mutans -stimulated in RAW 264.7 cells. RGE inhibited the expression of Ag I/II-N-induced pro-inflammatory mediators, both mRNA and protein synthesis levels, without any cytotoxic effects. Moreover, RGE significantly inhibited Ag I/II-N induced NF-κB translocation into the nucleus by preventing the degradation of inhibitor κB-α. In conclusion, RGE down regulates the expression of pro-inflammatory genes involved in the synthesis of NO and iNOS in Ag I/II-N-stimulated RAW 264.7 cells by suppressing NF-κB activity. 본 연구에서, 우리는 S. mutans Ag I/II 재조합단백질에 의해 유도되어진 염증유발 단백질의 발현에 홍삼 40% 에탄올 추출물의 효과를 알아보고자 하였다. 홍삼에탄올추출물은 Ag I/II 재조합단백질에 의해 유도되어진 염증유발물질들의 mRNA와 단백질의 발현을 억제하였다. 더불어 홍삼에탄올 추출물은 NF-κΒ p65가 핵내로 이용하는 것이 억제하였다. 결론적으로 홍삼 40%에탄올추출물은 NF-κB의 활성에 의해 NO 생성과 iNOS 발현이 조절되어지는 것으로 생각되어지며, 염증유발 관련 유전자들의 낮은 발현을 유도하는 것으로 관찰되어졌다.
온도에 의존하는 전기적 측정을 이용한 분자 메모리 소자의 전하 이동 메커니즘 분석
최경민,구자룡,김영관,권상직,Choi, Kyung-Min,Koo, Ja-Ryong,Kim, Young-Kwan,Kwon, Sang-Jik 한국전기전자재료학회 2008 전기전자재료학회논문지 Vol.21 No.7
A molecular memory device which has a structure of Al/$Al_2O_3$/ASA-15 LB monolayer/Ti/Al device, was fabricated. To study a charge transfer mechanism of molecular memory devices, current density-voltage (J-V) characteristics were measured at an increasing temperature range from 10 K to 300 K with an interval of 30 K. Strong temperature-dependent electrical property and tunneling through organic monolayer at low bias (below 0.5 V) were appeared. These experimental data were fitted by using a theoretical formula such as the Simmons model. In comparison between the theoretical and the experimental results, it was verified that the fitting results using the Simmons model about direct tunneling was fairly fitted below 0.5 V at both 300 K and 10 K. Hopping conduction was also dominant at all voltage range above 200 K due to charges trapped by defects located within the dielectric stack, including the $Al_2O_3$, organic monolayer and Ti interfaces.
Rhodobacter sphaeroides에 의한 ${\delta}-aminolevulinic{\;}acid$생산에 있어서 glutamic acid 및 감마 유도체의 영향
최경민,임왕진,황세영,Choi, Kyung-Min,Lim, Wang-Jin,Hwang, Se-Young 한국응용생명화학회 1993 Applied Biological Chemistry (Appl Biol Chem) Vol.36 No.3
The effect of ${\delta}-aminolevulinic\;acid$ (ALA) biosynthetic precursors and related compounds on the ALA productivity from a strain of Rhodobacter sphaeroides has been examined in vivo and in vitro systems. The relative ratios of ALA productivities by $C_{4}$- pathway to that by $C_{5}$-pathway in vivo and in vitro systems were 0.78 and 1.37, respectively. Although the expression rates of $C_{4}-$ and $C_{5}-pathways$ in cell-free systems prepared after precursors supplemented cultivations were increased 1.35 and 1.52 folds, respectively, the rate increase of $C_{4}-pathway$ was accompanied by the rate decrease of the $C_{5}-pathways$, and vice versa, as that the rates of both $C_{4}-$ and $C_{5}-pathways$ were lowered to be 0.91, 0.83, respectively. The order of cellular uptake rates of ${\gamma}-glutamyl$ derivatives relative to that found with L-glutamic acid were shown to be D-glutamic acid, 0.55: D-glutamine, 0.5: L-glutamine, 0.4: ${\gamma}-L-glutamyl$ ethylester, 0.3: GSH and Glu-pNA, 0. L and D configurations of glutamine were indicated as better substrates in vivo for ALA yields than those of glutamic acid, respectively. Rhodobacter sphaeroides 균주가 생산하는 ${\delta}-aminolevulinic\;acid(ALA)$의 생산성에 관하여 in vivo, in vitro 상에서 기질 및 관련 화합물을 이용하여 검토하였다. $C_5\;ALA$ 생합성계에 의한 ALA yield 대비의 $C_4$ 생합성계에 의한 비율은 in vive 상에서 0.78인 반면, in vitro 상에서의 비율은 1.37이었다.$C_4\;C_5$ 각 계의 기질 첨가 배양에 의한 cell-free system의 $C_4,\;C_5$ 계의 발현도는 미첨가 배양에 의한 system과 비교하여 각각 1.35, 1.52로 증가하였으나, 증가한 계에 대한 상대계의 발현도가 억제되어, $C_4$ 와 $C_5$ 계가 각각 0.91, 0.83으로 나타났다. ${\gamma}-Glutamyl\;derivatives$의 세포내 uptake rates는 L-glutamic acid를 기준으로 비교해서 D-glutamic acid, 0.55: D-glutamine, 0.5: L-glutamine, 0.4: ${\gamma}-L-glutamyl\;ethylester$, 0.3: GSH 및 Glu-pNA, 0의 순서를 보였다. Uptake rate와 관계없이 in vivo 상에서 L-과 D-glutamine이 L-, D-glutamic acid보다 균체 외 ALA의 생산에 있어서 각각 높은 yield의 효과를 보였다.
최경민,박응로,주홍신,양재경,이기영,이성택,이무춘,Choi, Kyung-Min,Park, Eung-Roh,Ju, Hong-Shin,Yang, Jae-Kyung,Lee, Ki-Young,Lee, Sung-Taik,Lee, Mu-Choon 유기성자원학회 1996 유기물자원화 Vol.4 No.2
자연계로부터 3종의 광합성 세균 strain KN 1-1, KN 2-1과 KN 2-3을 분리하여 돈분 폐수 처리에 대한 연구를 수행하였다. 유기산이 첨가된 배지에서 광합성 세균의 생육은 유기산을 첨가하지 않은 경우에 비해 2~3배 증가하였으며, bacteriochlorophyll a 함량도 1.5~2배까지 증가함을 보였다. 또한 축산 농가에서 직접 채취한 돈분 폐수에 광합성 세균을 첨가하였을 때 COD 감소율은 KN 1-1인 경우 80%, KN 2-1 89%, KN 2-3 75%를 나타내었다. Photosynthetic bacteria, strains KN 1-1, KN 2-1 and KN 2-3 were isolated from nature, and were studied for swine wastewater treatment. Growth of those photosynthetic bacteria were increased to 2~3 fold in organic-acid added medium(sodium acetate 1g, sodium propionate 1g and sodium butyrate 1g in Lascelles basal medium $1{\ell}$) than cultivation in Lascelles basal medium, and amount of bacteriochlorophyll a were increased to 1.5~2 fold. Chemical Oxygen Demand(COD) in swine wastewater using photosynthetic bacteria, strains KN 1-1, KN 2-1 and KN 2-3 were reduced 80%, 89% and 75%, respectively.
혐기정 소화 - 고온 호기법에 의한 유기성폐기물의 처리와 생성열의 재활용 검토
앙재경(Jae-Kyung Yang),최경민(Kyung-Min Choi) 유기성자원학회 2001 유기물자원화 Vol.9 No.1
본 연구에서는 유기성 폐기 물의 분해시 발생되는 열을 재활용하고 혐기성 소화액의 퇴비회를 위한 혐기성 소화- 고온 · 호기법 (Anaerobic Digestion-Thermophilic oxic process, ADTOP)을 고안하고, 유기물 분해와 수분의 증발 그리고 생성열의 적용성을 검토하였다. 유기성 폐기물인 중화요리 잔반은 TOP에 의해 완전처리가 가능하며, 최대 용적부하는 55kg/m3 . d, 투입된 수분은 거의 완전히 증발되었으며 탄소수지에 의한 탄소성 유기물의 이산화탄소 전환율은 90.5%이었다. 고온 혐 기성 소화를 위한 적정온도(약55 'C)를 유지하기 위한 최소용적부하는 45.0kg/m3. d이었다. 혐기성 소화조의 온도는 수리학적 체류시간이 짧아짐에 따라 지수적 온도강하를 나타내었으며 고온 혐 기성 소화를 위한 최소 HRT는 약 10 일 정도로 판단된다. 따라서 고온 · 호기법을 이용한 유기성폐기물의 처리시 발생되는 열에너지는 혐기성 소화와 같은 체류시간이 비교적 긴 공정에서 효과적일 것으로 판단된다. 혐기조의 유기물 부하 l.1kg-COD/m3• d, 고온 호기조 유기물의 투입량 50kg/m3, 공기 유입량 250ℓ/m3• min의 조건에서 혐기성 소화효율은 90 % 이상으로 나타났다.