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흰 쥐 해마에서 Midazolam 및 Propofol에 의한 GABA성 전류(GABAergic Currents)에 대한 비교
천상우 ( Sang Woo Chun ),손용 ( Yong Son ) 대한마취과학회 2009 Korean Journal of Anesthesiology Vol.56 No.6
Background: γ-Aminobutyric acid (GABA), the principal inhibitory neurotransmitter, activates persistent low amplitude tonic currents in several brain regions, in addition to conventional synaptic currents. Tonic conductance is highly sensitive to low concentrations of volatile anesthetics and therefore might contribute to amnestic properties. We compared the properties of GABAergic tonic currents mediated by sedative-amnestic midazolam and anesthetic propofol in rat hippocampal neurons. Methods: Patch clamp techniques were used to characterize the GABAergic currents recorded in CA1 pyramidal neurons in rat hippocampal slices. The amplitude of the tonic currents and the decay of miniature inhibitory postsynaptic currents (mIPSCs) were measured after administration of midazolam or propofol. Results: Both midazolam and propofol caused concentration dependent increases in the tonic currents. The enhancement of the tonic currents by midazolam concentrations of greater than 0.5 μM caused no further increase in current amplitude. Propofol continued to increase with concentrations over the range tested (0.1-10 μM). Low concentrations of midazolam 0.01 μM and propofol 0.5 μM selectively enhanced the tonic currents but failed to alter mIPSCs. Conclusions: Low concentrations of midazolam and propofol selectively enhanced the tonic currents but not synaptic currents of rat hippocampal pyramidal neurons. Unlike midazolam, the response to propofol did not become saturated and had a greater effect on the tonic currents. (Korean J Anesthesiol 2009; 56: 675~80)
TNBS 유발 대장염(大腸炎)에 의한 체중 및 혈액상 변화에 대한 인삼(人蔘) 약침(藥鍼)의 효과
김종성 ( Jong Sung Kim ),천상우 ( Sang Woo Chun ),박상연 ( Sang Yeon Park ),박성익 ( Sung Ik Park ),김경식 ( Kyung Sik Kim ),김재효 ( Jae Hyo Kim ),구성태 ( Sung Tae Koo ),손인철 ( In Chul Sohn ) 대한경락경혈학회 2006 Korean Journal of Acupuncture Vol.23 No.4
Objectives: The purpose of the present study is to evaluate the effects of herbal acupuncture with Ginseng Radix for the treatment to intestinal disease in the rat with 2, 4, 6-trinitrobenzenesulfonic acid (TNBS) induced colitis. Methods: All animals were subjected to the injection of saline (300 ㎕, 500 ㎕) for a study control or TNBS (300 ㎕, 500 ㎕) into the Lumen of the colon, 8cm proximal to the anus through the intestine. Ginseng Radix herbal acupuncture (20 ㎎/㎖, 0.4 ㎖) were injected to the both Hapgok (LI4) acupoints at the 2nd injection of TNBS in rats. Thus, the body weight, RBC count, WBC count, total protein, IgG Levels and IgM Levels were observed to study the effects of Ginseng Radix herbal acupuncture. Results: Ginseng Radix herbal acupuncture on Hapgok (LI4) for TNBS-induced colitis inhibited the body weight Loss rate but did not affect RBC and WBC counts. Furthermore, it inhibited the reduction of total protein concentration and serum IgG and IgM Levels in TNBS induced colitis were recovered. Conclusions: Herbal acupuncture with Ginseng Radix helps recover the TNBS-induced colonic damage and may be an important method for treatment of the colitis.
척수 아교질 세포의 흥분성과 세포내 칼슘농도에 대한 활성산소의 영향에대한 연구
이해인 ( Hae In Lee ),천상우 ( Sang Woo Chun ) 조선대학교 치의학연구원 2011 Oral Biology Research (Oral Biol Res) Vol.35 No.1
Substantia gelatinosa (SG) neurons, lamina II of the spinal dorsal horn, receive sensory informations from peripheral neurons directly and send the informations to upper brain regions. In recent studies, it is reported that reactive oxygen species (ROS) takes part in generation and transmission of pain, but these are the results from behavioral test and immunohistochemical study. In the present study, we recorded neuronal excitability to investigate the effects of tert-buthyl hydroperoxide (t-BuOOH), a kind of ROS, on transmission of pain in SG using the patch clamp technique. We also used calcium imaging technique to measure the changes of intracellular calcium concentration. Depolarization and action potential were evoked by perfusion of t-BuOOH, and these are suppressed by phenyl-N-tert-buthylnitrone, an antioxidant. When we treated t-BuOOH, intracellular calcium concentration was increased, but it was rather decreased in extracellular calcium free solution and on the condition of pretreated 2-aminoethoxydiphenylbolate, ruthenium red, and thapsigargin. We confirmed that t-BuOOH activates excitability of neurons in SG suggesting that ROS boosts nociception through central sensitization. This result is induced by increase of intracellular calcium, and the sources of calcium are from extracellular fluid and endoplasmic reticulum in neurons.
Retinoic Acid 가 사람 정상구강각화세포의 복제노화와 Apoptosis 에 미치는 효과
이동근(Dong Keun Lee),천상우(Sang Woo Chun),서세정(Se Jeong Seo),김경화(Kyong Hwa Kim),유용욱(Yong Ouk You),김수남(Su Nam Kim),민승기(Seung Ki Min),김강주(Kang Ju Kim) 대한악안면성형재건외과학회 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.6
연구목적 : 본 연구는 retinoic acid가 사람 정상구강각화세포의 복제노화와 apoptosis에 미치는 영향을 평가하기 위하여 시행하였다. 연구방법 : 사람 정상구강각화세포를 배양한 후,all-trans retinoic acid (RA)를 첨가하고,세포증식, population doubling level (PDL), senescence-associated β- galactosidase (SA-β-gal)활성, 세포생존율, apoptosis, western blot 분석 및 telomerase 활성을 측정하였다. 연구결과 : 저농도 (1nM)의 RA는 사람 정상구강각화세포의 증식을 현저히 증가시켰으며,복제노화의 지표가 되는 SA-β-gal 양성세포의 비율을 감소시켰다. 저농도의 RA는 세포생존율을 증가시키고 복제노화에 다다른 세포의 apoptosis를 억제하였다. 1nM의 RA 투여는 사람 정상구강각화세포의 축적 PDL을 증가시켰다. 대조군에서 SA-β-gal 양성세포는 PDL 증가에 따라 점진적으로 증가하였으나, 1nM의 RA투여시 PDL 증가에 의한 SA-β-gal 양성 세포의 축적이 억제되었다. 대조군에서 PDL 증가에 따라 p16INK4A 및 p110Rb 의 발현이 점진적으로 증가하였으나,1nM의 RA투여 시 PDL 증가에 의한 p16INK4A및 p110Rb 의 발현이 억제되었다. 대조군에서 telomerase 활성은 PDL 증가에 따라 점진적으로 억제되었으나, 1nM의 RA투여 시 PDL 증가에 의한 telomerase 활성은 점진적으로 증가되었다. 결론: 이러한 실험결과로 1nM의 RA투여가 배양된 사람 정상구강각화세포의 복제노화와 apoptosis를 억제시킬 수 있고,그 분자적 기전은 p16INK4A및 p110Rb 의 발현을 억제하고 telomerase 활성을 증가시킴으로써 이루어진다는 것을 알 수 있었다. N/A
천상우,박병림,한두석 원광대학교 치의학연구소 1992 圓光齒醫學 Vol.2 No.1
This study was designed to evaluate the effect of intermittent electrical stimulation on atrophied masseter muscle in the rat (230-250g). Growing Sprague-Dawley rats (80g) were assigned to either a control (CONT), a soft dieted (SOFT), or a soft dieted plus intermittent electrical stimulation (SOFT-ES) group. CONT group was continued to receive an ordinary hard rat diet and SOFT group received an ordinary diet in a ground form, mixed with water in a proportion of 2 : 5, for 6 weeks. After 6 weeks of soft diet feeding in SOFT group, mechanical properties of contraction in the masseter muscle were measured by means of isometric force transducer and then electrodes were implanted to the masseter muscle for intermittent electrical stimulation (SOFT-ES group). In SOFT-ES group, electrical stimulation was applied directly to the masseter muscle for 2 weeks at supramaximal intensity, 0.3ms, 20 Hz for 15 minutes every 6 hours. After electrical stimulation for 2 weeks, mechanical properties and fatigue tendency of the masseter muscle were observed. The results were as follows. 1. Twitch tension was no significant difference according to the mandibular position, however, tetanic tension at 11㎜ interincisal distance was larger than at 7㎜. 2. In SOFT group, tetanic tension and contraction time were decreased, and fatigue tendency was increased with the control group. 3. In SOFT-ES group, tetanic tension was increased and fatigue tendency was decresed compared with difference according to the mandibular position, however, tetanic the soft dieted group, and mechanical properties of muscle contraction were similiar to those of the control group. 4. Effects of electrical stimulation in atrophied muscle were more prominant in tension-related properties than speed-related properties. These data suggest that intermittent electrical stimulation may be useful in prevention and treatment of muscle atrophy.