Picibanil이 면양적혈구(緬羊赤血球) 감작(感作)마우스의 면역반응(免疫反應)에 미치는 영향(影響)

채효석,송희종,Chai, Hyo-seok,Song, Hee-jong 대한수의학회 1987 大韓獸醫學會誌 Vol.27 No.1

This experiment was performed on mice to investigate the effects of an immunopotentiator, picibanil(PC), on the immune responses such as phagocytic activity of reticuloen-dothelial(RE) system, E rosette formation rate of splenic lmphocytes and morphological changes of lymph node tissue. Groups of mice were treated with a single(1KE/kg BW) or sequential(0.1, 0.25 and 0.5KE/kg BW for successive 3 days) intravenous injections of PC. PC treated and untreated control mice were sensitized with 50% sheep erythrocyte suspension(0.2ml/mouse) at 1, 3, 5, 7 and 10 days after PC treatment. Functional and morphological examinations were carried out 5 days after sensitization. The following results were obtained: The phagocytic activity of RE system and the weight of liver and spleen were increased significantly at 3rd, 5th and 7th day. The peripheral polymorphonuclear leukocyte and percent of lymphocyte and monocyte were slightly increased. The rates of E rosette formation of splenic lymphotytes, sequential PC treated groups were more increased at 3rd and 5th day in sequential PC treated groups than in single treated groups. Thereafter it returned gradually to the control level by the time of 10th day. Microscopically primary lymph follicles with indistinct germinal center (GC) were partially disrupted and the parafollicular areas were consisted of the pyroninophilic cells in control group. In PC treated group, the parafollicular areas were markedly proliferated and developments of secondary lymph follicles with enlarged and prominent GC were more pronounced in the sequential injected groups compared to single injected groups. These results indicate that PC affected not only parafollicular area of the T-cell area, but also GC of the B-cell area. It suggests that PC may potentiate both cell mediated immunity and humoral immunity.

소의 순환혈액 및 림프조직내 T 및 B 림프구 분포

윤창용,김태중,채효석,김종면,송희종,Yoon, Chang-yong,Kim, Tae-Joong,Chai, Hyo-seok,Kim, Jong-Myeog,Song, Hee-jong 대한수의학회 1991 大韓獸醫學會誌 Vol.31 No.1

소의 순환혈액 및 림프조직내의 T 및 B 림프구의 분포를 파악하고자 소흉선 세포를 토끼에 과면역시켜 얻은 항혈청을 소의 적혈구, 간장분말 및 골수세포에 순차적으로 흡착시킨 항 흉선 세포혈청(RABTS)과 성숙 T 림프구에만 특이성을 가진 단클론항체 $BLT_1$ 및 B 림프구와 소수의 대식세포에 특이성을 가진 단클론항체 $_6E_{12}$를 1차 항체로 이용하여 ABPC법으로 T 및 B 림프구를 동정한 바 다음과 같은 결과를 얻었다. RABTS 및 $BLT_1$으로 동정한 순환혈액내 단핵세포(PB-MNCs) 중 T림프구의 비율은 각각 70.9${\pm}5.5%$ 및 $59.0{\pm}8.7%$이었으며, PB-MNCs중 nylon wool 비부착세포를 $BLT_1$ 및 $_6E_{12}$로 동정한 T 및 B 림프구의 비율은 각각 $91.6{\pm}1.0%$, $9.6{\pm}0.8%$이었다. 또한 $BLT_1$ 및 $_6E_{12}$로 동정한 서혜림프절, 장간막 림프절, 비장 및 흉선의 T/B림프구의 비율은 각각 $56.4{\pm}6.2/45.3{\pm}7.4%$, $55.6{\pm}7.7/42.3{\pm}5.8%$, $48.6{\pm}5.1/48.5{\pm}6.2%$ 및 $23.0{\pm}4.8/5.6{\pm}2.1%$이었으며, RABTS로 동정한 이들 림프조직내의 T림프구의 비율은 각각 $76.3{\pm}3.4%$, $74.2{\pm}8.2%$, $73.6{\pm}5.5%$ 및 $95.6{\pm}2.8%$이었다. 이상의 결과로 미루어 PB-MNCs네의 T림프구 분리는 nylon wool column법이 보다 효과적임을 알 수 있었다. This study was undertaken to identify the distribution of T and B lymphocytes in bovine peripheral blood and various lymphoid tissues by the method of ABPC using RABTS, $BLT_1$ and $_6E_{12}$ as primary antibodies. RABTS, $BLT_1$ and $_6E_{12}$ positive cells in PB-MNCs were 70.9${\pm}5.5%$, $59.0{\pm}8.7%$ and $23.0{\pm}8.7%$, respectively. $BLT_1$ and $_6E_{12}$ positive cells in nylon wool nonadherent cells of PB-MNCs were $91.6{\pm}1.0%$ and $9.6{\pm}0.8%$, respectively. In the lymphoid tissues such as inguinal lymph node, mesenteric lymph node, spleen and thymus, positive cells of RABTS were $76.3{\pm}3.4%$, $74.2{\pm}8.2%$, $73.6{\pm}5.5%$ and $95.6{\pm}2.8%$, those of $BLT_1$ were $56.4{\pm}6.2%$, $55.6{\pm}7.7%$, $48.6{\pm}5.1%$ and $23.0{\pm}4.8%$ and those of $_6E_{12}$ were $45.3{\pm}7.4%$, $42.3{\pm}5.8%$, $48.5{\pm}6.2%$ and $5.6{\pm}2.1%$, in order. These results are indicating that nylon wool column method is effective for separation of bovine ocytes.

한우(韓牛)에서 면역조절세포(免疫調節細胞)의 활성(活性)에 관한 연구(硏究) : I. 말초혈액(末梢血液) 임파구(淋巴球)의 E Rosette 형성능(形成能)

유남선,김종면,송희종,채효석,강명대,이주묵,You, Nam-sun,Kim, Jong-myeon,Song, Hee-jong,Chai, Hyo-seok,Kang, Myeong-dai,Lee, Ju-mook 대한수의학회 1987 大韓獸醫學會誌 Vol.27 No.2

The erythrocytes(E) rosette forming capacity of peripheral blood lymphocytes(PBL) in Korean native cattle was determined with 2-aminoethylisothiouronium bromide hydrobromide(AET) and dextran(Dex)-treated sheep erythrocytes(SRBC). To further standardize the assay, optimum concentration of AET and/or Dex-treatment and incubation time for rosette forming cell(RFC) counts were determined. In untreated SRBC resuspended in the rosetting medium(RPMI 1640 containing 10% FCS), PBL from 7 animals formed low percentage of rosettes($7.8{\pm}6.0%$). Both AET and Dex treatment not only enhanced the rosette formation but also made it easy to enumerate rosettes by increasing numbers of SRBC attached on them. SRBC treated with 0.1M AET for 20 min or 8% Dex formed the highest percentages of rosettes, ($35.7{\pm}6.0%$ and $48.3{\pm}4.7%$, respectively) and were used in subsequent studies. With SRBC treated with 0.1M AET for 20 min and suspended in 8% Dex, the maximum RFC observed were $56.6{\pm}6.8%$ in female and $65.8{\pm}6.3%$ in male and the rates of RFC did not change significantly between 3 and 20 hr incubation time at ${4^{\circ}C}$.

가축에서 대장균 감염증

송희종 ( Hee Jong Song ),채효석 ( Hyo Seok Chai ) 한국가축위생학회 1999 韓國家畜衛生學會誌 Vol.21 No.4

Escherichia coli is recovered from a wide variety of infections in many animals species. It may be a primary or secondary agent. Nursing and young animals are particularly susceptible, and urinary tract infections are frequent. The various serotypes of E coli are intestinal inhabitants of animals including humans and probably infect most mammals and birds ; therefore, they have a cosmopolitan distribution. Colibacillosis refers to any localized or systemic infection caused entirely or partly by E coli. Collibacillosis in mammals is most often a primary enteric disease, whereas collibacillosis in poultry is typically a secondary located or systemic disease occurring when host defenses have been impaired or overwhelmed. Other opportunistic bacteria, which can be identified by culture, may play a similar role to that of E coli in secondary infections. Collectively, infections caused by E coli are responsible for significant economic losses to the animal performance. From the standpoint of pathogenic mechanisms and diseases, four major categories of E coli are recognized enterotoxigenic(ETEC), enteropathogenic (EPEC), enteroinvasive(EIEC), and enterohemorrhagic(EHEC). In addition, two less-well-defined E coli categories are recognized in animals and humans : enteroaggregative and cytotoxin necrotizing factor-positive. The aforementioned categories are represented by different serotypes. Certain serotypes show a host preference and are encountered more frequently in some disease syndromes. Of the four major categories, ETEC is the most common cause of diarrhea in calves, lambs, and pigs. Strains in the other categories cause the less-common diarrhea and other disease syndromes. Enterotoxins and pilus antigens are the two most prominent virulence factors thus far identified for ETEC. Two enterotoxins, one heat-stable(ST) and one heat-labile(LT), are produced by enterotoxigenic strains of E coli not all culture produce both of these plasmid-based enterotoxins.

한우 림프구에 대한 단일항체 생산 1. T 림프구에 대한 단일항체

송희종,운재호,김태중,채효석,허부홍,최민순,Song, Hee-jong,Woon, Jae-ho,Kim, Tae-joong,Chai, Hyo-seok,Hur, Boo-hong,Choi, Min-soon 대한수의학회 1995 大韓獸醫學會誌 Vol.35 No.4

This study was undertaken to develop the monoclonal antibody(MAb) for lymphocytes of Korean native cattle by the cell hybridization of myeloma P3/NS-1/ 1-Ag-4-1 and spleen cells from BALB/c mice hyperimmunized with nylon wool column eluted peripheral T lymphocytes of Korean native cattle. The isotype of MAb KCT-14 against T lymphocyte was mouse $IgG_1$. KCT-14 positivity of mononuclear cells(MNC) from peripheral blood lymphocytes, nylon wool nonadherent and adherent-lymphocytes was 41.7%, 58.4% and 22.6%, respectively. And that of mesenteric lymph node-, spleen and thymus-MNC was 43.3%, 40.2% and 33.6%, respectively. Immunoperoxidase staining of frozen tissue sections showed that the MAb positive cells were located in the medulla of the thymus and in the paracortical area and the mantle zone of the germinal center in the lymph nodes. These results indicated that KCT-14 was one of the MAb for investigate of T lymphocyte subpopulations in the Korean native cattle.

산란계에서의 흉강내 기형종 발생보고

한규삼 ( Kyu Sam Han ),이성효 ( Sung Hyo Lee ),서문정 ( Moon Jeong Sub ),오언평 ( Un Pyong Oh ),채효석 ( Hyo Seok Chai ),유혜진 ( Hye Jin You ),임채웅 ( Chae Woong Lim ) 한국가축위생학회 1997 韓國家畜衛生學會誌 Vol.20 No.2

This article reports a spontaneous thoracic teratoma in a laying hen (Deklb brown warron). In this case, a 40 days old hen was submitted for necropsy as part of an investigation into a flock problem suspected Marek`s disease to Chonbuk Veterinary Service Laboratory in 1983. On the gross finding, mass was in the cranial subpulmonary cavity and attached to the vertebral column. It contained fully developed contour feathers. Histologically, feathers were arised from feather follicles complete with arrector plumi muscle, nerve, vessel and mucous gland composed with simple tall columnar epithelium. The outer surface of mass was lined by keratinized or nonkeratinized stratified squamous epithelium. It converted to pseudostratified ciliated columnar epithelium in some area. There were lymphocyte infiltration around gland tissue and eosinophilic intranuclear inclusion bodies in nonkeratinized epithelium. This thoracic teratoma was composed of ectodermal origin, squamous epithelium and nerve, and endodermal origin, mucous gland. This case in laying hen has never been reported in the literature in the world.

파라믹소바이러스 -분류, 구조, 게놈 및 코드화된 단백질-

송희종 ( Hee Jong Song ),어성국 ( Seong Kug Eo ),채효석 ( Hyo Seok Chai ) 한국가축위생학회 2002 韓國家畜衛生學會誌 Vol.25 No.2

도축 한우의 복강내 지방괴사증 발생과 병리조직학적 연구

최길성 ( Gil Seong Choi ),임채웅 ( Chae Woong Lim ),채효석 ( Hyo Seok Chai ),최인열 ( In Yeol Choi ),이성효 ( Sung Hyo Lee ),김추철 ( Chu Cheul Kim ),서문정 ( Moon Jeong Suh ) 한국가축위생학회 1999 韓國家畜衛生學會誌 Vol.21 No.1

The bovine fat necrosis is often seen as an incidental lesion in the adipose tissues of the abdominal cavity. Most of affected animals, however, have been detected at the slaughter house or routine necropsy. The purpose of this study was to examine the occurrence and distribution of abdominal fat necrosis in Korean native cattle, and its pathologic features. Postmortem inspection at an Chonju abattoir during a three-month period in 1997 detected at necrosis lesions in 67(6.6%) of the 1,012 animals received for slaughter. The occurrence was mainly in alimentary tracts and perirenal. Both sexes were affected, but the lesions were predominantly occurred in female. Gross lesions were white or yellowish in color and formed hard lumps irregular in shape ranging from small nodules to large solid masses. On the cut surface, lesions were occasionally seen chalky calcified granules and some of the lesions contained oil-like fluid. The surface of irregular shaped masses constricted kidneys and intestinal loops. Microscopically, the masses were encapsulated by thickened fibrous tissue, which infiltrated deeply and divided them into many irregular lobules. Initial lesions presented mild inflammatory cell and fibrous proliferation. It became fibroplasia in progressive lesions and resembled chronic lesion, eventhough no grossly apparent inflammatory reaction. The hard consistency of masses, due to fibroplasia, can compress the intestines, urinary organs, and reproductive organs.

파라믹소바이러스 -복제의 단계-

송희종 ( Hee Jong Song ),어성국 ( Seong Kug Eo ),조정곤 ( Jeong Gon Cho ),채효석 ( Hyo Seok Chai ) 한국가축위생학회 2002 韓國家畜衛生學會誌 Vol.25 No.3

도축돈의 혈청, 뇨 및 근육에서 sulfamethazine 잔류 조사

추금숙 ( Keum Sook Chu ),오언평 ( Un Pyong Oh ),최인열 ( In Yeol Choi ),송희종 ( Hee Jong Song ),채효석 ( Hyo Seok Chai ) 한국가축위생학회 1997 韓國家畜衛生學會誌 Vol.20 No.2

This study was undertaken to determined the sulfamethazine residues in urine, serum and muscle of slaughtered pigs(n=230) by the method of TLC, EEC-4-plate and HPLC. 1. Inhibition diameter characteristics of reference bacterial substance by EEC-4-plate method and antibacterial inhibition zone was appeared sulfonamides in BS pH 7.2 2. Residual sulfamethazine was detceted from 7 serum(3.04%) and 10 urine(4.35%) by TLC test and concentration of residues was higher in urine than serum. 3. Residual sulfamethazine was detected from 9 muscle(3.91%) by EEC-4-plate method. 4. Positive samples by TLC test and EEC-4-plate method were exceed 0.lppm quantitative analysis from 7 muscle(3.04%).