기주에 따른 목화검정진디벌(Ephedrus plagiator (Nees))과 진디벌(Aphidius ervi Haliday)의 생물학적 특성 비교

지창우,강은진,변영웅,김정환,최병렬,Ji, Chang Woo,Kang, Eun Jin,Byeon, Young-Woong,Kim, Jeong-Hwan,Choi, Byeong Ryeol 한국응용곤충학회 2014 한국응용곤충학회지 Vol.53 No.4

싸리수염진딧물(Aulacorthum solani) 방제를 위해 기생성 천적으로 국내에서 선발한 목화검정진디벌(Ephedrus plagiator)과 외국에서 판매되고 있는 진디벌(Aphidius ervi)을 비교 조사하였다. 성충 수명과 산란 기간, 머미수, 우화충수 등에 대한 우열성을 싸리수염진딧물과 완두수염진딧물(Acyrthosiphon pisum)을 대상으로 온도 15, 20, 25, $30^{\circ}C$와 광주기 16(L) : 8(D)의 조건에서 조사하였다. 성충 수명은 $15^{\circ}C$에서 목화검정진디벌이 싸리수염진딧물에서 5.9일로 진디벌 3.6일에 비해 2.3일 길었다. 산란 기간도 $15^{\circ}C$의 싸리수염진딧물에서 목화검정진디벌이 5.3일인 반면, 진디벌은 2.2일로 상대적으로 짧았다. 싸리수염진딧물에서 목화검정진디벌과 진디벌의 머미수는 목화검정진디벌이 15, 20, 25, $30^{\circ}C$에서 각각 71.5, 41.0, 15.7, 7.7개로 진디벌의 22.1 16.3 6.2, 0.4 보다 많이 기생하는 것으로 나타났다. 반면에 완두수염진딧물에서 목화검정진디벌의 머미수는 70.3, 69.8, 34.3, 8.4개로 93.4, 71.2, 34.8, 14.5로 조사된 진디벌의 머미수보다 낮았다. 싸리수염진딧물에서 목화검정진디벌의 우화충수는 42.1, 36, 11.6, 0로 나타나 $30^{\circ}C$의 높은 온도에서는 우화하지 않은 것을 알 수 있었다. 진디벌의 경우에는 우화 개체수가 각 온도에서 19.6, 13.5, 3.7, 0.1로 나타나 목화검정진디벌에 비해 상대적으로 적게 우화한 것으로 조사되었다. 두 종의 비교결과, 싸리수염진딧물에서는 목화검정진디벌이, 완두수염진딧물에서는 진디벌이 우수한 것으로 조사되었다. To investigate the biological control of Aulacorthum solani, a comparative analysis was carried out using an indigenous natural enemy, Ephedrus plagiator, and an exotic parasitoid, Aphidius ervi. Lifespan, spawning periods, number of mummies, number of offspring, and developmental periods of the two parasitoids on Aulacorthum solani and Acyrthosiphon pisum were studied at 15, 20, 25, and $30^{\circ}C$ with a photoperiod 16 h (L): 8 h (D). The lifespan of E. plagiator (5.3 days) was relatively higher than that of A. ervi (2.3 days) when these parasitoids were reared on A. solani at $15^{\circ}C$. Similarly, the spawning period of E. plagiator (5.3 days) was longer than that of A. ervi (2.2 days). When the two aphid parasitoids were provisioned with A. solani, the numbers of E. plagiator mummies at 15, 20, 25, and $30^{\circ}C$ were 71.5, 41, 15.7, and 7.7, respectively, whereas at the same temperatures, the numbers of A. ervi mummies were 22.1 16.3, 6.2, and 0.4, respectively. In contrast, when provision with A. pisum, the numbers of E. plagiator mummies at 15, 20, 25, and $30^{\circ}C$ were 70.3, 69.8, 34.3, and 8.4, whereas the numbers of A. ervi mummies were 93.4, 71.2, 34.8, and 14.5, respectively. The numbers of E. plagiator offspring emerging at 15, 20, 25, and $30^{\circ}C$ were 42.1, 36, 11.6, and 0, whereas the numbers of A. ervi offspring emerging were relatively lower at 19.6, 13.5, 3.7, and 0.1, respectively. By comparing these results, it can be concluded that E. plagiator is a more efficient parasitoid of A. solani, whereas A. ervi is more efficient on A. pisum.

국내 담수어류 45종의 먹이원 분석(문헌자료 분석을 중심으로)

지창우,이대성,이다영,곽인실,박영석,Ji, Chang Woo,Lee, Dae-Seong,Lee, Da-Yeong,Kwak, Ihn-Sil,Park, Young-Seuk 한국하천호수학회 2020 생태와 환경 Vol.53 No.4

We analyzed food sources of 45 fish species in 28 genera of 12 families based on literature data in freshwater ecosystems of South Korea. The food sources of 45 fish species included in a total of 26 phyla, 42 classes, 94 orders, 189 families and 294 genera. Among them, animal food sources were 16 phyla, 24 classes, 54 orders, 126 families and 212 genera, whereas plant food sources were relatively small with 10 phyla, 18 classes, 42 orders, 63 families and 82 genera. The animal food sources were classified into Arthropod, Insecta, Diptera and Chironomidae according to taxa. Meanwhile, Bacillariophyta, Bacillariophyceae, Cymbellales and Cymbellaceae were the most abundant among the plant sources. Self-Organized Map (SOM) and network analysis were conducted the food sources were classified into taxonomic groups and the feeding types of fish : 45 fish species were divided into five groups, characterizing 1) fishvores, 2) invertebratevores, planktivores including 3) zooplankton and 4) phytoplankton, and 5) omnivores. The network analysis presented link association between fishes and food sources. Macroinvertebrate including diptera and ephemeroptera were revealed as hub food sources based on network analysis. This literature study would expect that the application model with the food source of fish could be utilized for the evaluation of the food network or chain in freshwater ecosystems. 국내 담수 어류 12과, 28속, 45종의 먹이원을 문헌 조사한 결과, 동물류 먹이원은 16문, 24강, 54목, 126과, 212속으로 조사되었으며 식물류 먹이원은 10문, 18강, 42목, 63과, 82속으로 나타났다. 동물류 먹이원 중 가장 많은 어류가 섭식한 먹이원은 분류군에 따라 절지동물문, 곤충강, 파리목, 깔따구과로 조사되었으며, 식물류 먹이원은 돌말문, 윷돌말강, 반달돌말목, 반달돌말과로 조사되었다. SOM을 이용하여 45종 어류의 유형화 결과, 어류를 주로 포식하는 어류와 저서무척추동물을 포식하는 어류, 동물플랑크톤을 섭식하는 어류, 식물플랑크톤을 섭식하는 어류, 잡식성 어류 5가지 유형으로 나타났다. 네트워크 분석의 허브 점수가 높은 상위 5종의 어류는 블루길, 몰개, 수수미꾸리, 남방종개, 새코미꾸리 순으로 나타났으며 먹이원 중 허브 점수가 높은 상위 5 종류의 먹이원은 파리목, 하루살이목, 이지목, 김발돌말목, 쪽배돌말목 순으로 조사되었다.

가뭄에 의한 주암호의 수위 및 어류 군집 변동

오건희 ( Gun Hee Oh ),유태식 ( Tae-sik Yu ),지창우 ( Chang Woo Ji ),박영석 ( Young-seuk Park ),곽인실 ( Ihn-sil Kwak ) 한국수산과학회 2023 한국수산과학회지 Vol.56 No.6

Droughts can alter the dynamics of aquatic ecosystems, including fish communities. This study compared the varia-tions in fish communities in Juam reservoir before and after drought events. Fish sampling was conducted five times from April 2021 to April 2023 using three different sampling methods (kick net, cast net, and gill net). The water level in the reservoir reached its peak (103.73 EL.m, 62.2% capacity) in September 2021, before the drought, and dropped to its lowest point (88.84 EL.m, 17.6% capacity) in April 2023. The dissolved oxygen content in the reser-voir decreased from 27 to 6.3 mg/L between the period with the lowest water level (April 2023) and the period with the highest water level (September 2021). In September 2021, 466 fish were collected, but after one year of drought, the number of individuals decreased to 105. Further, the number of fish collected and water levels were positively correlated. Dominant species exhibited a population decline of over 60% with decreasing water levels. These findings highlight the importance of fishery resource management during drought periods.

Change in Spatial Dispersion of Daphnia magna (Cladocera: Daphniidae) Populations Exposed to Organophosphorus Insecticide, Diazinon

Sang-Hee Lee(이상희),Chang Woo Ji(지창우),Tae-Soo Chon(전태수) 환경독성보건학회 2009 환경독성보건학회지 Vol.24 No.3

We explored collective behaviors of indicator species to elucidate the effect of the chemical stress. After the treatments of an insecticide, diazinon, at low concentrations (1.0 and 10.0 ㎍/L), spatial dispersion patterns of Daphnia magna were checked in a test chamber. The I-index was used to characterize the movement data before (0~1 h) and after (1~2 h) the treatments in laboratory conditions. The slopes of the frequency distribution of I-index in semi-log scale decreased significantly, and the test populations appeared to be more dispersed with a lower degree of aggregation after the treatments. The index was feasible in indicating decrease in the ability of the specimens to keep desirable distances with neighbor individuals under chemical stress and showed a possibility of monitoring presence of toxic chemicals in environment through group behavior measurement.

저온저장이 기생성 천적인 콜레마니진디벌과 예쁜가는배고치벌의 기생 및 생존에 미치는 영향

서미자,김정환,서보윤,박홍현,지창우,박부용,이상구,조점래,Seo, Meeja,Kim, Jeong Hwan,Seo, Bo Yoon,Park, Hong Hyun,Ji, Chang Woo,Park, Bueyong,Lee, Sang Gu,Cho, Jum Rae 한국응용곤충학회 2019 한국응용곤충학회지 Vol.58 No.4

When Aphidius colemani mummies were stored at four different temperatures (6, 8, 10 and 12 ± 1℃), they could be stored at 8℃ for 10 days with over 50% adult emergence. A number of mummies of Rhopalosiphum padi parasitized by A. colemani adults emerged from mummies after 8℃ storage clearly reduced with increasing duration of storage and especially declined with storage longer than 13 days showing < 20% parasitism. However, there were no differences in aphid parasitisms by A. colemani on 3 to 10 days after storage. Over 3 day storage at 8℃ was adversely affected on survival of A. colemani adults. Therefore, cold storage of A. colemani adults is considered as an unsuitable method for mass production of biological control agent. When 2-day-old Meteorus pulchricornis cocoons were stored at 6, 8 and 10 ± 1℃, it was possible to be stored up to 63 days at 8℃, showing the highest emergence. With increasing of cold storage duration at 8℃, parasitism on 2^nd and 3^rd larval instar of Spodoptera litura by stored M. pulchricornis significantly reduced. However, parasitic rate by M. pulchricornis stored for 2 weeks was similar to that by 1 week stored ones. Therefore, M. pulchricornis cocoons could be stored up to 2 weeks at 8℃ for stockpiling before release. 콜레마니진디벌 머미는 처리한 온도조건(6, 8, 10 및 12 ± 1℃) 중, 8℃에서 저장 10일 까지 50% 이상의 우화율을 보였다. 머미 우화율이 가장 높았던 8℃에서 콜레마니진디벌 머미의 저장기간에 따른 기생된 기장테두리진딧물 머미 수를 조사한 결과, 저장 후 진딧물 기생율이 급격히 떨어지는 것이 확인되었으며, 13일 이상의 저장할 경우 평균 기생율이 20%를 넘지 못하였다. 머미 상태로 저장기간 3일부터 10일까지는 기생율의 차이를 보이지 않았다. 콜레마니진디벌 성충은 3일 이상의 저장은 생존율이 급격히 감소하는 경향을 보여 성충태로의 저온저장은 불가능할 것으로 판단되었다. 고치 형성 후 2일차 된 예쁜가는배고치벌 고치를 6, 8 및 10 ± 1℃ 에서의 저장 기간에 따른 우화율을 조사한 결과, 8℃에서 가장 높은 우화율을 나타냈으며 최대 63일까지 저장이 가능하였다. 8℃ 조건에서 저장기간이 길어질수록 산란기주인 담배거세미나방 2, 3령 유충의 치사율과 고치 형성 수가 급격히 감소하는 경향이었으며, 최적 저장기간은 2주 정도인 것으로 판단되었다.

Toxicity of Plant Essential Oils and Their Spray Formulations against the Citrus Flatid Planthopper Metcalfa pruinosa Say (Hemiptera: Flatidae)

Jun-Ran Kim(김준란),Chang Woo Ji(지창우),Bo Yoon Seo(서보윤),Chang Gyu Park(박창규),Kwan-Seok Lee(이관석),Sang-Guei Lee(이상계) 한국농약과학회 2013 농약과학회지 Vol.17 No.4

지표생물의 독성물질 반응 행동에 대한 수리적 평가

전태수(Tae-Soo Chon),지창우(Chang Woo Ji) 환경독성보건학회 2008 환경독성보건학회지 Vol.23 No.4

Various methods for detecting changes in response behaviors of indicator specimens are presented for monitoring effects of toxic treatments. The movement patterns of individuals are quantitatively characterized by statistical (i.e .. ANOVA, multivariate analysis) and computational (i.e., fractal dimension, Fourier transform) methods. Extraction of information in complex behavioral data is further illustrated by techniques in ecological informatics. Multi-Layer Perceptron and Self-Organizing Map are applied for detection and patterning of response behaviors of indicator specimens. The recent techniques of Wavelet analysis and line detection by Recurrent Self-Organizing Map are additionally discussed as an efficient tool for checking time-series movement data. Behavioral monitoring could be established as new methodology in integrative ecological assessment, filling the gap between large-scale (e.g., community structure) and small-scale (e.g., molecular response) measurements.

시설재배 고추에 발생하는 4종의 주요 해충에 대한 천적이용 효과

김정환,변영웅,최만영,지창우,허수영,박은미,강은진,Kim, Jeong-Hwan,Byeon, Young-Woong,Choi, Man-Young,Ji, Chang-Woo,Heo, Su-Yeong,Park, Eun-Mi,Kang, Eun-Jin 한국응용곤충학회 2012 한국응용곤충학회지 Vol.51 No.2

반촉성재배 시설고추에 발생하는 4종의 해충에 대한 천적이용 효과를 660 $m^2$의 면적에서 조사하였다. 진딧물은 3포트의 진디벌-뱅커플랜트와 콜레마니진디벌(Aphidius colemani)을 $m^2$당 4월16일 0.8 마리와 4월 23일 2.3 마리를 방사하여 진딧물을 성공적으로 방제할 수 있었다. 총채벌레 방제를 위해 미끌애꽃노린재(Orius laevigatus)를 5월 11일과 6월 12일 $m^2$당 총 3.0 마리를 방사하여 작기 동안 총채벌레 밀도를 꽃 당 0.3 마리 이내로 억제하였다. 지중해이리응애(Amblyseius swirskii)를 5월 11일과 5월 30일 $m^2$당 총 113.7 마리를 방사하여 작기동안 담배가루 이 밀도를 트랩 당 171.0마리 이내, 차응애 밀도는 잎 당 0.8 마리 이내로 억제하였다. 차먼지응애는 지중해이리응애 방사 12일 후에 완전방제 되었다. 4종 해충의 방제에 소요된 천적사용 비용은 660 $m^2$당 42만원이었다. The effect of natural enemies on four major pests of hot pepper was tested in greenhouses. The aphids were successfully controlled by introducing three Aphidius colemani-banker plants, and releasing 23.3 wasps per $m^2$ on April 16 and 23 wasps per $m^2$ in a greenhouse of 660 $m^2$. To control thrips, Orius laevigatus was released twice, 3.0 bugs per $m^2$ at a time(May $11^{th}$ and June $12^{th}$). The thrips population was controlled within 0.3 thrips per flower during the growing season. To control two species of mites, Tetranychus kanzawai and Polyphagotarsonemus latus, and the silverleaf whitefly, Bemisia tabaci, a total of 113.7 individuals of Amblyseius swirskii were released twice(May $11^{th}$ and $30^{th}$). The densities of B. tabaci and T. kanzawai were kept within 171.0 individuals/trap and 0.8 individual/leaf, respectively. P. latus was suppressed completely twelve days after release. The cost of the released natural enemies to control the four arthropod pests in this study was 420,000 Won per 660 $m^2$.

꽃매미 알 기생천적인 꽃매미벼룩좀벌의 대량사육기술: 산누에나방과 꽃매미 알 활용 방법

서미자,김정환,서보윤,박창규,최병렬,김광호,지창우,조점래,Seo, Meeja,Kim, Jeong Hwan,Seo, Bo Yoon,Park, Changgyu,Choi, Byeong Ryeol,Kim, Kwang Ho,Ji, Chang Woo,Cho, Jum Rae 한국응용곤충학회 2018 한국응용곤충학회지 Vol.57 No.4

꽃매미벼룩좀벌(Anastatus orientalis)을 실내에서 대량사육 할 수 있는 대체기주를 선발하기 위하여 꽃매미와 썩덩나무노린재(Halyomopha halys), 톱다리개미허리노린재(Riptortus pedestris), 매미나방(Lymantria dispar), 참나무산누에나방(Antheraea yamamai), 귀뚜라미(Verlarifictorus spp.), 집파리(Musca domestica), 산누에나방(Antheraea pernyi) 총 8 종의 알 또는 번데기를 이용하여 검토하였다. 그 결과 꽃매미 알과 산누에나방의 미성숙 알에서는 꽃매미벼룩좀벌의 산란과 우화가 확인되었으나 그 밖에 종의 알과 번데기에서는 산란을 확인할 수 없었다. 대체기주로 선발된 산누에나방은 참나무 잎으로 사육하였으며, 7월과10월 상순에 번데기를 수확하여 우화 후 4~5일경에 복부가 불룩한 산란 직전의 암컷 성충만을 선발하여 $1{\sim}5^{\circ}C$ 냉장고에 보관하였으며, 저온에 보관했던 암컷의 복부를 절개하여, 암컷 한 마리당 150~200개의 알을 얻었다. 꽃매미 알과 산누에나방의 미성숙 알에서 꽃매미벼룩좀벌의 발육기간은 각각 평균 36.8일과 36.1일이었으며, 24시간동안 암컷 한 마리가 산란한 알의 수는 각각 평균 4.2개와 3.4개로 통계적으로는 유의한 차이가 없었다. 한편, 꽃매미벼룩좀벌 암컷 성충의 수명은 벌꿀을 먹이로 제공 하였을 때, 평균 64.3일까지 생존하는 것으로 확인되었다. 산누에나방의 미성숙 알을 대량으로 확보하고 벌꿀을 먹이로 제공하면 꽃매미벼룩좀벌을 실내에서 대량사육 하는 것이 가능하고 야외방사를 통해 꽃매미의 생물적 방제에 활용할 수 있을 것으로 기대된다. Eggs, immature eggs, and pupae of 8 different insects (Halyomopha halys, Riptortus pedestris, Lymantria dispar, Antheraea yamamai, Verlarifictorus spp, Antheraea pernyi, and Musca domestica) including Lycorma delicatura were used to select the alternative host for laboratory mass rearing of A. orientalis. Except L. delicatula's eggs and immature eggs of A. pernyi, other 7 tested insects were not parasitized by A. orientalis. A. pernyi was reared with oak tree leaves and its cocoons were harvested on mid-July and early October. On 4 or 5 days after emergence, only female adults showing swollen abdomen were collected and stored at $1{\sim}5^{\circ}C$. We could get 150~200 eggs per one female by dissecting the female's abdomen. For examining the possibility for laboratory mass rearing of A. orientalis with A. pernyi's immature eggs, developmental periods from egg to pupa between the two different hosts were compared. Developmental periods were 36.1 days on immature eggs of A. pernyi and 36.8 days on an original host's eggs, respectively. The number of parasitized eggs by A. orientalis' female for 24 h was 3.4 on immature eggs of A. pernyi and 4.2 on an original host's eggs, respectively. However, there were no significant statistical differences in developmental period and parasitization between the two hosts. By supplying honeyed water to newly emerged female parasitoids, it was able to maximize their longevities up to 64.3 days after emergence. Therefore, our results support potential for laboratory mass-rearing of A. orientalis using A. pernyi's immature eggs as an alternative host.

꼬마남생이무당벌레[Propylea japonica (Thunberg)]의 온도발육모형

이상구,박부용,전성욱,정인홍,박세근,김정환,지창우,이상범,Lee, Sang-Ku,Park, Bueyong,Jeon, Sung-Wook,Jeong, In-Hong,Park, Se-Keun,Kim, Jeong-Hwan,Ji, Chang-Woo,Lee, Sang-Bum 한국유기농업학회 2017 韓國有機農業學會誌 Vol.25 No.4

The present study was conducted to investigate the developmental characteristics of Propylea japonica Thunberg (Coleoptera : Coccinellidae), a predator of aphids under laboratory conditions. The development times of the egg and immature stages of Propylea japonica were investigated at ten constant temperatures(15, 17.5, 20, 22.5, 25, 27.5, 30, 32.5, 35 and $38{\pm}1^{\circ}C$; 14L:10D; $60{\pm}5%$ RH). Development time was longest at $17.5^{\circ}C$. The developmental periods of P. japonica from the time of egg oviposited to adult emergence was 36.9 days at $17.5^{\circ}C$, and it decreased ash temperature increasing. Threshold temperature of development and degree days of the P. japonica from egg to adult were $11.9^{\circ}C$ and 196.0 respectively. The Briere 2 and Lactin 2 modes were best fitted for all development stages. The distribution of completion of each development stage was explain relatively well that 2-parameter, 3-parameter and Logistic model except egg and pupal stage.