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박공지붕형 저층건축물의 지붕 및 벽면에 작용하는 피크외압의 분포 특성
조원근,원종호,하영철,Jo, Won Geun,Won, Jong Ho,Ha, Young Cheol 한국강구조학회 2009 韓國鋼構造學會 論文集 Vol.21 No.3
산업용 건축물은 일반적으로 박공지붕형 저층의 형태로 많이 건설된다. 본 연구는 박공지붕형 저층건물의 풍압분포와 특성을 알아보기 위하여 풍동실험을 수행하였다. 풍동실험은 경사각 변화에 따른 외장재용 피크외압계수 분포양상을 알아보기 위하여 동일한 폭과 높이, 길이를 가지지만 지붕 경사각이 서로 다른 7가지의 모형을 제작하여 실험하였다. 실험결과 지붕과 벽면의 모서리 부분에서 최대 피크외압계수가 나타났으며, 이를 KBC-2005 및 각국의 기준과 비교하여 보았다. 실험에 의한 피크외압계수는 AIJ-2004와 매우 유사하게 나타났지만 KBC -2005보다는 다소 크게 나타났다. The low-rise buildings with gable roofs are commonly used in a number of industries. In order to study the characteristics of peak external pressure coefficient on low-rise buildings with gable roofs, wind-tunnel test have been carried out. Wind-induced pressures were measured simultaneously at many points on wind-pressure models, typical of simple low-rise buildings with gable roofs, which have seven different roof slope with constant width(D), height(H), and length(D). The pressure measurements were made in one kind of turbulent boundary layer, which simulated the natural winds over typical suburban terrains at a geometric scale of 1/150. The results indicate that peak external pressure coefficient on the roof and wall edges were increased. The results compared with wind standard of KBC-2005 and standards of various nations. The comparative resultant, experimental result appeared very similar at AIJ-2004. But the results were somewhat larger then wind standard of KBC-2005.
가축 유래 staphylococcus aureus의 enterotoxin 산생과 plasmid profile에 관한 연구 V. R-plasmid의 분리 및 전이
강호조(Ho Jo Kang),김용환(Young Hwan Kim),손원근(Won Geun Son),강광식(Gwang Sig Kang),이제용(Je Young Lee) 한국예방수의학회 1992 예방수의학회지 Vol.16 No.1
Animal source strains of Staphylococcus aureus were studies for the profiles of plasmids and the transfer of resistance plasmid by conjugation. A total of 50 isolates of S. aureus were subjected to the test for the presence of plasmid DNA. The isolates of bulk milk, chicken and Pig origin had 1 to 5 plasmid element. The plasmids recovered from these organisms had molecular weight ranging from 1.3 to 63 kb, 0.94 to 63 kb and 1.1 to 44 kb, respectively. In the strains isolated from bulk milk, the majority of the isolated plasmids was large molecular weight and they had one plasmid element. On the other hand, small molecular weight plasmids were predominant and they had multiple plasmid element in the isolates of chicken origin. Tetracycline and gentamicin resistant plasmid of S. aureus were transferable between S. aureus by filter mating method, but in the broth mating method were not transferable plasmids. The frequencies of tetracycline plasmid transfer between S. aureus strains were 2.0-5.2×10⁻⁷, and gentamicin plasmids transfer were 1.2×10⁻⁸.
마우스에서 listeria monocytogenes의 장관정착과 그 결정요인 III. 장관정착에 미치는 요인시험
손원근(Won Geun Son),강호조(Ho Jo Kang) 한국예방수의학회 1995 예방수의학회지 Vol.19 No.2
In SPF-ICR mice inoculated orally with Listeria monocytogenes investigated were effect of gastric juice, neutralizer of gastric acid, hemolysin and p60 for bacterial colonization. In in vitro stomach contents of heat-treated, HCI-treated to pH<3.0, and normal contents as control, bacterial counts in heat-treated samples were similar to those in control group 8 hrs after inoculation. Bacterial growth was inhibited markedly In HCI-treated samples at 2 hrs after inoculation. Bacterial counts respectively of 3.16 log CFU /g and 2.66 log CFU /g in stomach of mice treated with sodium bicarbonate and ranitidine-HCI were prominent than those in control group(1.0 log CFU /g) at 2 hrs after inoculation. Moreover, the cecal colonization levels in sodium bicarbonate-treated group(5.57 log CFU /g) or ranitidine-HCI-treated group(5.13 log CFU /g) were higher than those of control group(2.27 log CFU /g). The cecal colonization level by L. monocytogenes strain Mackaness harvouring virulent factors as hemolysin and p60 was higher than those by such strains deficient for virulent factors as RI (hemolysin⁺p60⁻) or M3(hemolysin⁻ p60⁺).
Listeria spp. 표면단백질의 분석 및 면역원성
강호조(Ho Jo Kang),손원근(Won Geun Son) 한국예방수의학회 1996 예방수의학회지 Vol.20 No.1
This study is conducted to investigate specific surface proteins (SP) of Listeria spp. with SDS-PAGE and immunoblotting, and irnmunogenicity of specific SP in mice and Korean native goat immunized with those of Listeria monocytogenes. Profiles of L. monocytogenes were different from those of other species of Listeria. Surface protein with molecular weights of 62 and 68kd were usually found in all L. monocytogenes serovars. Strains of sv. 4b were characterized by the association of two major characteristic bands at 76 and 78kd, and sv 1/2 were characterized by the association of two major bands at 60 and 97kd. In spite of the weak similarity in SDS patterns, irnmunoblotting showed a large antigenic community among serovars or species: the 4b antiserum reacted in the profiles of all Listeria spp. with two major band at 76 and 78kd, and the 1/2b antiserum reacted in the profiles of all Listeria spp. with two major band at 68 and 76kd. Antigenic titers of specific SP in mice and in Korean native goat were 5,120 for 78kd, 2,560 for 62kd(in goat, 1,280), and 640 for 97kd.
도계장 유래 닭고기와 부산물 및 환경재료에서 Listeria spp의 분리 및 분리균의 특성 II. 분리한 L monocytogenes의 혈청형과 항균제에 대한 감수성
손원근,강호조,Son, Won-geun,Kang, Ho-jo 대한수의학회 1991 大韓獸醫學會誌 Vol.31 No.3
To investigate the epidemiological aspects of listeriosis, serotypes of L monocytogenes and antimicrobial susceptibilities of Listeria spp isolated from chicken carcases and chicken slaughter house environmental specimens were determined. Of 28 L monocytogenes strains, 12 strains(42.9%) were serotype 4, and the remaining 16 strains were untypable. Peak distributions of minimum inhibitory concentration$({\mu}g/ml)$ of the isolates were $0.78{\mu}g/ml$ for ampicillin, $0.39{\mu}g/ml$ for erythromycin and penicillin G, $1.56{\mu}g/ml$ for tetracycline and $6.25{\mu}g/ml$ or $12.5{\mu}g/ml$ for chloramphenicol, and $3.13{\mu}g/ml$ to > $100{\mu}g/ml$ for kanamycin and neomycin. Most of 214 isolates were sensitive to ampicillin and erythromycin, but 20. 1~78. 0% of the strains were resistant to tetracycline, kanamycin, penicillin-G and neomycin. Single or double drug resistance were observed in 75.8% of the resistant strains. The most common resistance patterns were Nm P-G(37.4%) in double pattern and P-G(23.7%) in single pattern.
중합효소연쇄반응을 이용한 실험적 리스테리아 감염증의 신속진단
강호조,이성미,석주명,이덕규,손원근,Kang, Ho-jo,Lee, Seong-mi,Suk, Ju-myoung,Lee, Deog-kyu,Son, Won-geun 대한수의학회 1998 大韓獸醫學會誌 Vol.38 No.3
The polymerase chain reaction(PCR) assay was used for rapid diagnosis from blood and organ samples experimentally infected with Listeria monocytogenes. This method used a pair of primers based on a unique region in the 16S rRNA sequence of L monocytogenes. Procedure A was based on dilution of the blood sample followed by lysis of bacterial cell and direct analysis of the lysate with PCR. In artificially infected blood samples with L monocytogenes, it was possible to detect fewer than 40 cells per ml of blood. However, L monocytogenes was detected low rates on infected organs by the direct PCR. In procedure B, enrichment cultivation was used to increase numbers of bacteria before lysis and PCR. L monocytogenes was detected from 23 samples of 24 liver and spleen, respectively, and 18 samples of 24 blood were found to be positive by PCR on a subset of 72 organ samples, whereas L monocytogenes were detected on 63 organ samples in classical culture technique. It was required to analyze including enrichment steps were 6h and 18h on the procedure A and B, respectively.
도계장 유래 닭고기와 부산물 및 환경재료에서 Listeria spp의 분리 및 분리균의 특성 I. Listeria spp의 분리
손원근,강호조,Son, Won-geun,Kang, Ho-jo 대한수의학회 1991 大韓獸醫學會誌 Vol.31 No.3
To investigate the epidemiological trait of listeriosis, Listeria spp were isolated from poultry meat, products and environmental specimens in chicken slaughterhouse. Also determined were isolation rates by the different enrichment procedures, the biochemical properties of isolates. In a total of 307 samples including poultry meat, liver, feathers, feces, chiller water, scalding water overflow and slaughterhouse floor, Listeria spp were isolated predominantly from scalding water overflow (90.0%), body skin before washing (66.7%), liver (20.0%) and feathers(15.0%) However, few Listeria spp were isolated from body skin after washing and feces. The higher isolation rates were obtained in the secondary enrichment procedure (7.2%) than in the primary enrichment (3.9%); after stored the secondary enrichment cultures for 2 weeks at $4^{\circ}C$, Listeria spp were present in 9.8%. The majority of the isolated Listeria spp were identical to those of the standards strains in biochemical and cultural properties. Overall, Listeria spp were present in 13.4% of the specimens tested, and were in order of prevalence of L innocua(11.1%), L monocytogenes(3.3%), L grayi(0.7%) and L murrayi(0.3%).
가축 유래 staphylococcus aureus의 enterotoxin 산생과 plasmid profile에 관한 연구 II. 분리균주의 enterotoxin 산생
강호조(Ho Jo KANG),최홍근(Hong Geun CHOE),손원근(Won Geun SON),Hideo IGARASHI(五十嵐英夫) 한국예방수의학회 1991 예방수의학회지 Vol.15 No.1
The present study was conducted to investigate the enterotoxin types of Staphylococcus aureus isolated from bulk milk, Korean native cattle, pigs and poultry meat. Enterotoxins were produced by 88.0% of the organisms isolated from bulk milk, 76.2% from cattle, 64.7% from pigs and 22.5% from poultry meat. Enterotoxin type C and D were most common, being 42.0% and 12.8%, respectively. Enterotoxins were produced by all the strains of coagulase types Ⅱ, Ⅲ, Ⅳ and Ⅴ, 94.4% of type Ⅵ and 77.4% of type Ⅶ, while only 36.8% of the mixed type ⅡㆍⅢ produced enterotoxins. Commonly, coagulase types Ⅳ, Ⅴ, Ⅵ and Ⅶ strins produced enterotoxin C, and coagulase typis Ⅱ and ㆍⅢ strains produced enterotoxin D.
강호조(Ho Jo Kang),손원근(Won Geun Son),이제용(Je Young Lee),강광식(Gwang Sig Kang) 한국예방수의학회 1993 예방수의학회지 Vol.17 No.1
This study was conducted to investigate the colonization characteristics of Listeria monocytogenes in chickens. A total of 70 chickens were challenged orally with either 10², 10⁴, and 10⁶ cells of L. monocytogenes strain Scott A (serotype 4b) and wild strain(serotypel) at newly hatched chicks. Seven days after the challenge, the cecum, duodenum, spleen, liver and cloacal swabs were sampled from each chick and were analysed for the presence of L. monocytogenes. Serotype 4b strain was a dose-related colonization response(10⁶〉10²) ; and more recoveries were obtained from the cecum, duodenum and cloacal swabs than from the spleen and liver. L. monocytogenes was discharged(1.0-5.7 CFU log₁₀) for 7 days after the challenge, and after that, organisms were not recovered from any cloacal swabs.