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국내 해산양식어 조피볼락에서 분리된 수생버나바이러스 GC-1의 VP2 발현
조성준,성환우,이윤정,김재홍,강신영,Joh, Seong-joon,Sung, Haan-woo,Lee, Yun-jeong,Kim, Jae-hong,Kang, Shien-young 대한수의학회 2003 大韓獸醫學會誌 Vol.43 No.3
The VP2 gene of aquatic birnavirus, Korean isolate (GC-1) was cloned and expressed using the baculovirus expression system. The VP2 gene and VP2 partial gene, which contained a neutralizing epitope, were constructed for recombinant transfer vectors, for baculovirus expression. The expressed recombinant proteins were confirmed by indirect immuno fluorescence antibody (IFA), SDS-PAGE and Western blot. The level of expression was checked at regular time using IFA and Western blot. To measure the neutralizing activity of recombinant proteins against GC-1 strain, the antisera against recombinant proteins were produced by using guinea pigs. The result showed that the antisera neutralized the GC-1 strain. However, the neutralizing titer was higher in antisera against the VP2 gene expressed recombinant protein than that of VP2 partial gene recombinant protein.
양식 넙치에서 Vbrio anguillarum 및 Ichthyobodo sp.에 의한 혼합 감염증의 발생 및 치료
장환,문진산,조성준,김지연,손성완,Jang, Hwan,Moon, Jin-San,Joh, Seong-Joon,Kim, Ji-Yeon,Son, Seong-Wan 대한수의학회 2007 大韓獸醫學會誌 Vol.47 No.3
The high mortality (65.5%) of young olive flounder (Paralichthys olivaceus) showing skin ulcers occurred in fish obtained from a commercial cultured farm at Donghae on the eastern coast of Korea at March 2006. The dorsal skin showed ulcer lesions and large amount of mucus. We isolated Ichthyobodo sp. from the gill, and Vibrio anguillarum from the liver and kidney of dead and diseased fish. All of the fish infected with the V anguillarum and Ichthyobodo sp. were treated with 100 ppm formalin for 1 h, and treated with oxolinic acid for 4 h at $22^{\circ}C$, respectively. The formalin treated group was observed the low mortality (2.9%) when compared with high mortality of non-treated (82.9%), and of antibiotic treated group (85.9%) against mixed infections by Vibrio anguillarum and Ichthyobodo sp. organisms. We confirmed complete elimination against mixed infections of Vibrio anguillarum and Ichthyobodo sp. with 100 ppm formalin treatment.
2006~2007년 국내 종오리 농장에서의 HPAI 증례 보고
김민정 ( Min Jeong Kim ),조성준 ( Seong Joon Joh ),김민철 ( Min Chul Kim ),이윤정 ( Youn Jeong Lee ),정옥미 ( Ok Mi Jeong ),우계형 ( Gye Hyeong Woo ),권준헌 ( Jun Hun Kwon ),모인필 ( In Pil Mo ) 한국예방수의학회(구 한국수의공중보건학회) 2011 예방수의학회지 Vol.35 No.2
Korea had experienced second epidemic highly pathogenic avian influenza (HPAI), and there were the seven affected farms, including two breeder duck farms, from 22nd November 2006 to 6th March 2007. Here, we reported the clinico-pathological characteristics of domestic breeder ducks farms naturally infected with HPAI virus (H5N1). Clinically, the most ducks showed various signs from depression, decreased egg production and feed consumption to even, death. The most commonly gross changes were hepatomegaly, splenomegaly, petechial and ecchymotic hemorrhage on the liver surface, a white stripe on the cardiac muscle, and severely hemorrhagic and deformed eggs. The most significant histopathological changes were necrosis of various cells such as neuron, lymphocytes, cardiac myocytes, hepatocytes, blood vessels and pancreatic acinar epithelium. The viral antigen was mainly detected in the endothelium of blood vessels of various organs and tissues, peripheral nerves and neuronal cells. Based on the above results, we identified that HPAI H5N1 induced systemic infection in the adult breeder ducks.
β-용혈성 Streptococcus iniae 불활화백신의 넙치에 대한 효능 평가
문진산,장환,김지연,조성준,김민정,손성완,Moon, Jin-San,Jang, Hwan,Kim, Ji-Yeon,Joh, Seong-Joon,Kim, Min-Jeong,Son, Seong-Wan 대한수의학회 2007 大韓獸醫學會誌 Vol.47 No.3
Olive flounder, Paralichthys olivaceus is one of the most important cultured fish in Korea, its farming has been negatively impacted by viral, bacterial and parasitic diseases. Streptococcal infection was considered as a serious problem because of significant economic losses in olive flounder farm industry. The development and evaluation of vaccine for protection against infection by this agent were required. We evaluated the safety and efficacy of ${\beta}$-hemolytic Streptococcus (S.) iniae vaccine on olive flounder Three hundreds of flounders (weight $119.8{\pm}20.7g$, body length $22.6{\pm}1.4cm$) were reared in 0.5 tons aquaria in land-marine tank system. Seawater was provided from the sea of Inchon in Korea, and water temperature was set to $22^{\circ}C$ and $25^{\circ}C$ in the vaccination and challenge test, respectively. We used the formalin-inactivated ${\beta}$-hemolytic S. iniae (F2K) vaccine (M VAC INIAE; Kyoritsu seiyaku, Japan) originated in Japan. The vaccine was intraperitoneally administered to fish. Both of vaccinated group and control group were challenged with intraperitoneally injection by virulent S. iniae SI-36 isolates with $1.0{\times}10^7CFU/fish$ at 3 weeks after vaccination. Difference on mortality of control and vaccinated group (90.0 and 15.0%, 76.5 and 8.0% respectively) at two trials were found significant (p<0.05), and relative percent survival were 83.4% and 89.5%, respectively. The dead fishes were showed dark pigmentation of skin, abdominal extension, hemorrhagic ascites, and liver necrosis, and isolated the S. iniae strain from ascites, liver and kidney. We confirmed the safety and efficacy of ${\beta}$-hemolytic S. iniae vaccine by determinations of the optimal management condition and artificial challenge test in olive flounder.
서해안 양식 넙치에서 Neoheterobothrium 감염증의 발생 및 치료
장환,문진산,김지연,조성준,손성완,Jang, Hwan,Moon, Jin-San,Kim, Ji-Yeon,Joh, Seong-Joon,Son, Seong-Wan 대한수의학회 2007 大韓獸醫學會誌 Vol.47 No.3
Neoheterobothrium hirame (N. hirame) is a large blood-feed gill-worm infecting the highly prized olive flounder. The present study was reported the occurrence and elimination of Neoheterobothrium infection from young cultured oliver flounder in the western coast of Korea. Flounder (weight 18-25 g, length 10-15 cm) were obtained from a cultured farm at Taean of Korea at May 2006. A lot of flounder died with serious anemia. We isolated N. hirame from the buccal cavity wall of dead flounder. Adult parasites were 5-15 mm in total length, with 4 pairs of clamps on the pedunculated haptor and isthmus region embedded in host tissue, while immature and sub-adults were identified the attachment to the gill filaments, rakers and arches with the clamps. All fish with infection were treated with 8% NaCl-supplemented seawater for 1 and 10 min at $22^{\circ}C$, respectively. The significant reduction in mortality (6.7%) was observed among fish treated with 8% salt water for 10 minutes, when compared with that of non-treatment group (100%).