Effects of Human Platelet Transforming Growth Factor-$\beta$ on Replication and Collagen Synthesis in Primarily Cultured Mesangial Cell

조성우,박수길,김금이,이희봉,이재담,Cho, Sung-Woo,Park, Su-Kil,Kim, Geum-Yi,Lee, Hee-Bong,Lee, Jae-Dam 생화학분자생물학회 1992 한국생화학회지 Vol.25 No.3

쥐의 mesangial 세포를 초회 배양하여 Transforming Growth Factor-$\beta$(TGF-$\beta$)가 세포의 복제 및 collagen 합성에 미치는 영향을 조사하였다. TGF-$\beta$는 사람 혈소판에서 산성 에탄올 침전법 및 FPLC를 이용한 젤 크로마토그래피의 방법으로 정제되었다. TGF-$\beta$가 mesangial 세포를 복제 및 collagen 합성에 미치는 영향은 TGF-$\beta$ 농도와 세포의 밀도에 따라 달라졌다. Confluent 세포의 경우 0.1~15 ng/ml의 농도에서 TGF-$\beta$는 mesangial 세포의 복제를 35%까지 감소시켰다. Collagen 합성은 0.5 ng/ml의 TGF-$\beta$ 농도에서는 2배로 증가되었으나 그 이상의 농도에서는 감소하기 시작하는 두 가지의 조절 양상을 보였다. 그러나 subconfluent 세포의 경우에는 세포의 복제 빛 collagen 합성 모두에 TGF-$\beta$는 아무런 효과를 나타내지 못하였다. 본 연구결과는 TGF-$\beta$가 glomerulosclerosis에 있어서 중요한 역할을 하리라는 가능성을 제시해주고 있다. The effect of TGF-$\beta$ on cell replication and collagen synthesis in primarily cultured mesangial cells from rats was studied in vitro. The TGF-$\beta$ was purified from human platelets by acidic ethanol extraction and gel filtration with FPLC. The effect of TGF-$\beta$ on the DNA synthesis and the collagen synthesis in mesangial cells depended on both TGF-$\beta$ concentration and cell density in monolayer culture. Concentrations of TGF-$\beta$ ranging from 0.1~15 ng/ml were evaluated for their effects upon DNA synthesis. In confluent cells, when compared to the growth rate in standard medium, TGF-$\beta$ concentrations in the range 0.1~15 ng/ml reduced a cell proliferation up to 35%, suggesting inhibition of cell division. The effect of TGF-$\beta$ on collagen synthesis in mesangial cells was biphasic. In confluent cells, the addition of TGF-$\beta$ induced a 2-fold increase in the production of collagen when compared with cells grown in standard medium. The maximal effect of TGF-$\beta$ occurred at 0.5 ng/ml. At higher concentrations there was a decrease in the action of TGF-$\beta$ with the effects on collagen production. In subconfluent cells, however, TGF-$\beta$ showed no significant effects on the DNA synthesis and the collagen synthesis at all concentrations. These results support that TGF-$\beta$ may play an important role as a multifunctional regulator in the pathogenesis of glomerulosclerosis.

환경부하 저감을 위한 기후순응주거 사례연구

조성우,오세규,Cho, Sung-Woo,Oh, Se-Gyu 한국주거학회 2013 한국주거학회 논문집 Vol.24 No.6

The concept of sustainable development to preserve natural environment through economy in resources and energy is gradually distinguished since environmental problems as a series of air pollution and global warming take place now. Building occupies more than 1/3 of energy consumption in the whole world. Energy consumption of buildings caused a quick increase in the amount of energy use by air conditioning, which has a lot of effects on exhaustion of fossil energy as well as an increase in environmental load. Therefore, this research intends to suggest characteristics and direction for the designing of acclimatization's housing through the case study on acclimatization's housing. Acclimatization's housing is designed to reduce building load from the designing stage unlike green home or low-energy apartment. Also, factors which are commonly applied to acclimatization's housing are applied complexly by design strategic factors of Heating, Cooling and Lighting. The applied design factors make effects mutually and complexly. In addition, design strategy showed different priority by designing direction.

EnergyPlus를 이용한 수평형의 지열 히트펌프와 어스튜브를 조합한 시스템의 성능 검토

조성우,Cho, Sung-Woo 한국지열에너지학회 2017 한국지열에너지학회논문집 Vol.13 No.2

This study is performed to performance of the combined system the GSHP (Ground Source Heat Pump) system with the Earth tube system using EnergyPlus program. The Earth tube system using fan is characteristics as supply lower (higher) air temperature than outdoor air temperature in cooling and heating seasons, the GSHP system is characteristics as small indoor air temperature variation range. As the results of Earth tube + GSHP system simulation, GSHP power can be reduced than the GSHP single operation as 17.3% in cooling seasons and 32.5% in heating seasons, the GSHP design capacity can be replaced more small size.

사람 혈소판에서 분리한 Transforming Growth Factor - β가 초회 배양된 Mesangial 세포의 복제 및 Collagen 합성에 미치는 영향

조성우,박수길,김금이,이희봉,이재담 ( Sung Woo Cho,Su Kil Park,Geum Yi Kim,Hee Bong Lee,Jae Dam Lee ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.3

The effect of TGF-β on cell replication and collagen synthesis in primarily cultured mesangial cells from rats was studied in vitro. The TGF-β was purified from human platelets by acidic ethanol extraction and gel filtration with FPLC. The effect of TGF-β on the DNA synthesis and the collagen synthesis in mesangial cells depended on both TGF-β concentration and cell density in monolayer culture. Concentrations of TGF-β ranging from 0.1∼15 ng/ml were evaluated for their effects upon DNA synthesis. In confluent cells, when compared to the growth rate in standard medium, TGF-β concentrations in the range 0.1∼15 ng/ml reduced a cell proliferation up to 35%, suggesting inhibition of cell division. The effect of TGF-β on collagen synthesis in mesangial cells was biphasic. In confluent cells, the addition of TGF-β induced a 2-fold increase in the production of collagen when compared with cells grown in standard medium. The maximal effect of TGF-β occurred at 0.5 ng/ml. At higher concentrations there was a decrease in the action of TGF-β with the effects on collagen production. In subconfluent cells, however, TGF-β showed no significant effects on the DNA synthesis and the collagen synthesis at all concentrations. These results support that TGF-β may play an important role as a multifunctional regulator in the pathogenesis of glomerulosclerosis. v

전통주거건축 공간구성에 따른 안방창호 구성에 관한 연구 - 전라남도 지역 중상류 주택을 중심으로 -

조성우,이재홍,문출성,천득염,Cho, Sung-Woo,Lee, Jae-Hong,Moon, Chul-Seong,Cheon, Deuk-Youm 한국주거학회 2014 한국주거학회 논문집 Vol.25 No.3

Korean traditional house has inherented construction characteristics that contain human requirements with the adaptation to nature's order. As for traditional buildings, there are a lot of facilities like palaces, Buddhist temples, Confucianism architectures, government facilities, etc. Among them, housing construction is not only closely connected to our lives, but also plays an important role to connect our lives from the past to present. The master room in a traditional korean house is responsible for playing a center role of the residential life which implies the traditional values responsible for multi-functions and it is also the sphere of the living space. Therefore, this thesis considers the residential environmental adjustment method and the behaviors centered around the main buildings of Jeonnam Province. And also investigates the main reason for windows and doors formation. From this, we would like to further evaluate the master room life, and the inner space constructions following the spacial and organizational analysis of windows and doors. We would like to analyze the space organization methods, environmental adjustment methods and the usages of traditional buildings. Using the modern interpretation as basis, we would like to use the foundation materials to reflect single family housing plans.

꽃노랑총채벌레와 대만총채벌레에 대한 51종의 살충제 감수성 평가

조성우,경예진,조선란,신소은,정대훈,김성일,박근호,이승주,이영수,김민기,조인준,구현나,김현경,김길하,Cho, Sung Woo,Kyung, Yejin,Cho, Sun-Ran,Shin, Soeun,Jeong, Dae Hun,Kim, Sung Il,Park, Geun-Ho,Lee, Seung-Ju,Lee, Young-Su,Kim, Min-Ki,Jo, In-Jun 한국응용곤충학회 2018 한국응용곤충학회지 Vol.57 No.3

시판되고 있는 51종(단제 21종과 합제 30종)의 살충제를 이용한 꽃노랑총채벌레와 대만총채벌레 성충에 대한 약제 감수성을 검토한 결과 90% 이상의 살충률을 보인 약제 15종을 선발하였으며, 시판되고 있는 합제에서 많은 유효성분들이 오남용되고 있다고 보여진다. 2종의 총채벌레에 대한 살충제 감수성을 비교한 결과 모든 약제에 대하여 대만총채벌레가 꽃노랑총채벌레에 비해 높은 감수성을 보이기 때문에 꽃노랑총채벌레에 효과 있는 약제로 동시방제가 가능할 것으로 판단된다. 꽃노랑총채벌레를 대상으로 선발된 15종의 약제를 이용하여 약효발현속도($LT_{50}$과 $LT_{95}$), 침투이행성, 잔효성을 비교하였다. 약효 발현속도를 $LT_{95}$ (hour)값으로 비교했을 때, chlorpyrifos WP, chlorpyrifos + diflubenzuron WP가 2시간 이내로 가장 빨랐고, spinetoram WG은 62.3시간으로 가장 느렸다. Chlorfenapyr SC에서 뿌리와 엽면을 통한 침투이행 효과가 나타났고, spinetoram WG에서 엽면을 통한 침투이행 효과만이 나타났다. 약제 잔효성 실험에서 chlorfenapyr SC는 14일, benfuracarb WG와 chlorpyrifos WP는 3일까지 효과가 지속되었다. 꽃노랑총채벌레 야외집단(화훼재배지와 과채류재배지)간의 감수성 비교에서는 화훼재배지에서 채집된 집단이 더 낮은 약제 감수성을 보였다. The susceptibility of the western flower thrips, Frankliniella occidentalis and garden thrips, Frankliniella intonsa was evaluated using 51 commercial insecticides. 15 kinds of insecticides which showed more than 90% mortality against both thrips, F. occidentalis and F. intonsa was selected. Many active ingredients were misused and abused in commercial mixture formulation insecticides. Since the F. intonsa was more susceptible than F. occidentalis, it was considered that both thrips can be controlled by insecticides that showed insecticidal activity on the F. occidentalis. Lethal time ($LT_{50}$ and $LT_{95}$), systemic toxicity and residual toxicity of selected insecticides were compared. Both chlorpyrifos WP and chlorpyrifos + diflubenzuron WP revealed the fastest toxicity within 2 h ($LT_{95}$), while spinetoram WG revealed the slowest toxicity as 62.3 h ($LT_{95}$). Chlorfenapyr SC showed toxicity at foliar and drenching application while spinetoram WG was toxic only in foliar application. Chlorfenapyr SC showed residual effect at 3, 5, 7, 10, 15 days after treatment and both benfuracarb WG and chlorpyrifos WP showed residual effect at 3 days after treatment. As a result of treatment of selected insecticides for field population of F. occidentalis, the population collected from horticultural crops showed lower susceptibility than the population collected from vegetable crops.

PubMed를 통한 소아질환에 대한 추나 치료 연구의 최신 동향

조성우,Cho, Sung-Woo 대한한방소아과학회 2010 대한한방소아과학회지 Vol.24 No.2

Objectives We aimed reviewing studies related to the treatment of children by Chiropractic treatment. Methods We referred to PubMed by using searching keywords of chiropractic and the terms of pediatrics, such as child, children, juvenile, pediatric, infant. Twenty four papers were found. Results 1. We found twenty four papers about pediatric and chiropractic. 2. Chiropractic was used in various pediatric diseases and was shown to be effective. 3. There were several views on safety and effectiveness of pediatric chiropractic. Conclusions Applying of pediatric chiropractic was broad, and it was effectively cure for pediatric diseases.

교통사고 환자에 대한 진단 및 치료의 경향성 연구 -국내에서 발표된 학위지 밀 학술논문을 중심으로-

조성우,강연경,장동호,이인선,Cho, Sung-Woo,Kang, Yeon-Kyeong,Chang, Dong-Ho,Lee, In-Seon 척추신경추나의학회 2009 척추신경추나의학회지 Vol.4 No.2

Objectives : The purpose of this study is finding tendencies of diagnosis and treatment of the traffic accidental patients. Methods : We reviewed and analyzed all theses published by Korean research institution. And these theses were classified by year, field of study, reviewed by focusing on diagnosis and evaluation, treatment type. Results : 1. 72 articles were published since 1993. 2. Classified by the major field of study, the korean academy of oriental rehabilitation medicine accounted for 33 papers. 3. 7-Zone-Diagnostic system and Minnesota Multiphasic Personality Inventory were studied mostly. 4. To add pharmacopuncture or chuna treatments are effective. 5. Traffic accidental patients get satisfaction about Oriental medicine therapy but methods of medical treatment are restrictively. Conclusions : Oriental medicine treatment is the effective way to cure traffic accidental sequela, but we need further studies.

광릉긴나무좀, 왕녹나무좀, 노랑애나무좀 성충에 대한 포스핀의 훈증활성

조성우,김성일,김현경,김길하,Cho, Sung Woo,Kim, Sung Il,Kim, Hyun Kyung,Kim, Gil-Hah 한국응용곤충학회 2019 한국응용곤충학회지 Vol.58 No.1

많은 산림해충들은 목재검역에서 문제가 되고 있어 포스핀($PH_3$)을 이용하여 광릉긴나무좀, 왕녹나무좀, 노랑애나무좀의 성충에 대한 살충 활성을 조사하였다. 3종의 나무좀류에 대하여 99% 살충활성을 보이는 LCT값은 각각 3.192 (광릉긴나무좀), 0.994 (왕녹나무좀), $0.501mg{\cdot}h/L$ (노랑애나무좀) 순으로 나타났다. 포스핀을 처리한 3종의 나무좀류는 시간이 지남에 따라 살충활성도 증가하였다. 특히 광릉긴나무좀에서는 0.4 mg/L 이상의 농도에서 약제처리 7일 후에 100%의 사충률을 보였다. 이러한 결과로 볼 때 포스핀이 3종의 목재해충 성충에 대해 메칠브로마이드의 대체훈증제로 이용될 수 있을 것으로 판단된다. Many forest pests have caused problems for wood quarantine. The fumigation activity of phosphine ($PH_3$) was examined for the adults of three wood-boring insect pests. The $LCT_{99}$ values for Platypus koryoensis, Cryphalus fulvus, and Xyleborus mutilates were 3.192, 0.994, and $0.501mg{\cdot}h/L$ at $20^{\circ}C$, respectively. The effectiveness of $PH_3$ was increasingly time dependent for all doses tested in all three species. In particular, P. koryoensis showed 100% mortality at doses higher than 0.4 mg/L 7 days after fumigation. These results indicate that methyl bromide could be substituted for $PH_3$ for adults of these three species of wood pest.

한방병원에 입원한 교통사고 환자 34명에 대한 임상적 고찰

조성우,고경훈,남정훈,김봉현,이인선,Cho, Sung-Woo,Go, Kyung-Hoon,Nam, Jung-Hun,Kim, Bong-Hyun,Lee, In-Seon 척추신경추나의학회 2006 척추신경추나의학회지 Vol.1 No.2

Objective: The purpose of this study is finding tendencies admission patients to orient medical hospital due to Traffic Accident. Method: We studied 34 cases who were admitted in Dong-eui University Oriental Hospital from 1st, December, 2003 to the 30st, May, 2005. And we came to some conclusion about clinical tendencies as follows. Results and Conclusions: 1. Women had occupied more than men, And 10 persons in 30th were the most distribution of age. 2. Most patients used western medical hospital as primary hospital and after several days they started to search for orient medical treatments. 3. In the diagnosis through radiological test, cervical spine sprain was most(19 cases, 55.6%). The others were lumbar spine sprain(14 cases, 41.2%), fracture of other bone{4 cases, 11.8%), fracture of spine(3 cases, 8.8%), Intercranial hemorrhage(3 cases, 8.8%). 4. In chief complaint, low back pain was most(19 cases, 55.9%), The others were neck stiffness(17 cases, 50.0%), headache(13 cases, 38.2%), lower limb pain(9 cases, 26.5%). 5. Simple Disease had higher than complex disese at effective rate. 6. Both $43{\sim}60$ days admission and $15{\sim}21$ days admission were the best effective. $8{\sim}14$ days admission was next effective. 7. Early visit made more effective.