소의 제4위전위증 교정을 위한 봉봉합법

조명래,Cho Myoung-Rae 한국임상수의학회 1992 한국임상수의학회지 Vol.9 No.1

Surgical treatment was performed in 507 heads out of 1078 dairly cows which were diagnosed as abomasal displacement. Out of 507 heads. bar suture was performed in 280 heads. Twelve heads out of 280 heads(4.9%) recurred. Incorrective suture of the reticulum or pylorus instead of abomasum occurred in 3 cows. The cows in which abdominal wall was sutured but the suture was not inserted into the abomasum were 4 heads. The cows in which bar was broken are 4 heads. A cow suffered peritonitis after bar suture. Bar suture technique is thought to be a good method as a first choice for the correction of abomasal displacement.

Attachment of Pasteuria penetrans Endospores to Meloidogyne spp. Juveniles Affected by Temperatures and the Nematode species

조명래,김형환,강택준,안승준,양창열,전성욱,Cho, Myoung Rae,Kim, Hyung Hwan,Kang, Taek Joon,Ahn, Seung Joon,Yang, Chang Yul,Jeon, Sung Wook Korean Society of Applied Entomology 2013 한국응용곤충학회지 Vol.52 No.2

선충절대기생세균(Pasteuria penetrans)이 감염되어 있는 온실토양을 이용하여 세균의 내생포자가 땅콩뿌리혹선충(Meloidogyne arenaria) 유충 표면에 부착하는데 대한 온도의 영향에 대해 시험하였다. 갓 부화된 뿌리혹선충 2령충(J2)을 페트리디쉬 내의 토양에 접종한 후 $20^{\circ}C$, $25^{\circ}C$, $30^{\circ}C$, $35^{\circ}C$에서 7일간 처리하였다. 모든 온도에서 내생포자의 J2 부착률은 모두 100%로 나타났으나 J2당 내생포자 부착수는 $25^{\circ}C$에서 28.3개로 가장 많았으며 $30^{\circ}C$, $20^{\circ}C$ 및 $35^{\circ}C$에서 각각 J2 당 20.2, 18.6 및 13.6개로 낮아졌다. J2를 접종하기 전에 세균이 있는 토양을 온도별로 10일간 전처리하였을 때 내생포자 부착률은 실온에서의 60%에 비해 $-300^{\circ}C$, $4^{\circ}C$, $40^{\circ}C$, $50^{\circ}C$ 및 $100^{\circ}C$에서 각각 25.0, 31.7, 8.3, 5.0 및 0%로 현저하게 낮아졌다. J2 당 내생포자 부착수도 실온에서의 5.3개에 비해 $-30^{\circ}C$, $40^{\circ}C$, $40^{\circ}C$, $50^{\circ}C$ 및 $100^{\circ}C$에서 각각 3.5, 4.3, 1, 1, 0개로 적었다. P. penetrans 세균의 내생포자를 뿌리혹선충 종별로 J2에 부착 시험한 결과 땅콩뿌리혹선충에서는 100%였으나 당근뿌리혹선충(M. hapla)과 고구마뿌리혹선충(M. incognita)에서는 모두 0%로 본 균주는 뿌리혹선충 종에 대해 기주선호성을 가진 것으로 나타났다. A greenhouse soil infested with an obligate nematode parasitic bacterium, Pasteuria penetrans, was used to test the effect of temperatures on the endospore attachment to root-knot nematode, Meloidogyne arenaria, juveniles (J2). Freshly hatched J2s were inoculated to the soil in petri dish and incubated under different temperatures of $20^{\circ}C$, $25^{\circ}C$, $30^{\circ}C$, and $35^{\circ}C$ for 7 days. The endospore attachment rates were 100% in all the temperatures, while the number of endospores attached per J2 was highest in $25^{\circ}C$ with 28.3 endospores/J2 followed by 20.2, 18.6, and 13.6 in $30^{\circ}C$, $20^{\circ}C$, and $35^{\circ}C$, respectively. When the soil was pre-treated under different temperatures before the J2 inoculation, the endospore attachment rates significantly decreased from 60% in room temperature to $25^{\circ}C$, 31.7, 8.3, 5.0, and 0% after the soil incubation in $-30^{\circ}C$, $4^{\circ}C$, $40^{\circ}C$, $50^{\circ}C$, and $100^{\circ}C$ for 10 days, respectively. The endospore numbers attached per J2 were 3.5, 4.3, 1, 1, and 0 when the soil was pre-treated in $-30^{\circ}C$, $4^{\circ}C$, $40^{\circ}C$, $50^{\circ}C$, and $100^{\circ}C$, respectively, which were lower than 5.3/J2 of room temperature treated soil. The P. penetrans isolate in the soil showed nematode species-specific endospore attachment characteristics with 100% attachment rate only on M. arenaria J2s while the rates were 0% on M. hapla and M. incognita J2s.

여뀌섭취가 반추수에 비치는 임상병리학적 영향

조명래,한홍율,Cho Myoung-Rae,Han Hong-Ryul 한국임상수의학회 1989 한국임상수의학회지 Vol.6 No.2

Waterpepper is a weed which grows on damp soil. especially near swamps, and in shallow water of ponds and ditches. It Is widespread throughout the country In abundant colonies. In the present experiments, possible toxic effects of waterpepper were investigated in ruminants. Pour cows were fed waterpepper ad libitum or by force in the from of green forage, hay and/or powder, 8 goats were administered in the form of methanol extract, and 4 goats, crude juice, into the lumen. Clinical signs were examined as well as urinalysis, hematology, serum chemical analysis, pH/blood gas analysis and chclinesterase activities following administration of waterpepper. Six goats which were administered the methanol extract or crude juice were sacrificed for pathological examinations., In addition to the clini copathological examinations, the chemical constituents of waterpepper were qualitively analyzed from the methanol extract and the Effects of the waterpepper crude juice were examined on the motility of rabbit duodenum and uterus. It is revealed that waterpepper contains steroids, terpenoids, flavonoids, tannin and essential oils in the methanol extract and nitrates in the crude juice. The crude juice of waterpepper relaxed the rabbit uterine and duodenal smooth muscles. The constraction of duodenum by acetylcholine or BaCl$_2$ were partially inhibited by pretreatment of the crude juice. However, the relaxation of duodenum by the crude juice was not blocked by the pretreatments of phenoxybenzamlne, propranolol, cocaine, reserpine and tetrodotoxin. The constituents of waterpepper to evoke elaxation of duodenal smooth muscle were stable to heat. The cows administered waterpepper showed common clinical symptoms such as acrid expression, restlessness, dullness, inappetence, anorexia, severe diarrhea, mild bloat and left displacement of abomasum, while bloody feces was shown in a cow. The goats administered the mothanol extract showed common clinical signs such as acrid expression, restlessness, dullness, inappetence and soft feces, while bloody feces was shown in a goat, A goat adminstered the crude juice showde bloody feces and diarrhea. Respiratory rates and heart beats were increased along with diarrhea in the experimental cows. The erythrocyte counts and MCHC were decreased whereas PCV, MCV and neutrophils were increased in the cows administered waterpepper. In goats administered methanol extract, there were decreases in erythrocytes, PCV and hemoglobin content, and an increase in MCHC. The goats ingester with the crude juice showed negligible changes in hematologic values compared with control group which was administered the same amount of water instead of the crude juice. The contents of serum calcium, Inorganic phosphorus, magnesium, Iron, glucose, cholesterol, total protein, triglycerides and phospholipids were tended to decrease in cows. In goats serum iron, glucose, triglycerides, cholesterol, BUN and phospholipids content were decreased while the content of sodium and chloride were increased after administration of the methamol extract The goats ingested with the crude juice did not show significant changes in serum chemical analysis. Even though there were some pathological findings such as hyperemia in the small intestines and kidneys and swelling of liver parenchymal cells, the values of serum AST, ALT, LDH, alkaline phosphatase, total bilirubin and creatinine did not change significantly. While proteins, hemoglobin and blood were detected in the urine of cows, urine pH, ketone bodies, glucose, bilirubin and urobilinogen content were normal or undetected. There were no significant changes in pH/bolld gas analysis data of cows and cholinesterase activities of plasma and erythrocytes of cows and goats ingested with waterpepper or the methanol extract. It is concluded that waterpepper irritates the gatrointestinal system, causes abdominal pain, relaxes the gastrointestinal smooth muscle and dilatates blood vessels supplie

유우의 번식능률에 관한 연구 -분만간격, 임신시간, 첫 수정일수, 공태일수-

조명래,권오경,Cho Myoung-Rae,Kwon Oh-Kyeong 대한수의사회 1985 대한수의사회지 Vol.21 No.3

The survey was carried out to investigate calving interval, gestation period, the interval between parturition and first breeding and the duration from delivery to conception in the total of 320 heads of Holstein cows being fed in the suburbs of Suwon. Th

감자 주산지 식물기생선충 분포와 뿌리혹선층의 PCR 동정

조명래,이영규,김점순,유동림,Cho Myoung-Rae,Lee Young-Gyu,Kim Jum-Soon,Yoo Dong-Lim 한국응용곤충학회 2006 한국응용곤충학회지 Vol.45 No.1

국내 주요감자재배지의 식물기생선충 발생상을 밝히기 위해 2004년과 2005년에 제주, 예산, 김천, 고령, 홍천, 평창, 김제, 밀양, 남원, 강릉, 인제 등 11개 감자 주산지를 대상으로 선충속별 종류와 밀도를 조사하였다. 채집한 토양시료 50개 중 30개에서 뿌리혹선충 유충이 검출되었으며 밀도는 12-69마리/토양 100cc 정도로 비교적 낮았다. 식물기생선충류 중 Pratylenchus sp., Helicotylenchus sp, Ditylenchus sp., Tylenchus sp., and Tylenchorhynchus sp.가 지역별로 검출되었으나 대부분 밀도는 매우 낮게 나타났다. 채집한 토양을 토마토 뿌리에 접종 후 뿌리혹선충을 배양하여 PCR-RLFP에 의한 정밀동정 결과 제주, 밀양, 고령에서 채집한 뿌리혹선충 암컷에서 500 bp의 PCR 산물을 얻었으며 제한 효소 Dra I을 처리한 결과 290 및 230 bp의 밴드가 형성되어 당근뿌리혹선충(Meloidogyne hapla)으로 동정하였다. This study was conducted to get basic information on the occurrence of plant-parasitic nematodes for the establishment of nematode management strategy in major potato production areas in Korea. Nationwide soil collection was done in 11 areas of Cheju, Yesan, Gimchun, Goryoung, Hong chun, Pyungchang, Gimjae, Milyang, Namwon, Gangnung, and Inje in 2004-2005. Root-hot nematode juveniles(J2) were detected in 30 samples among the 50 samples. The average density was 12-69 J2/100cc soil. Pratylenchus sp., Helicotylenchus sp., Ditylenchus sp., Tylenchus sp., and Tylenchorhynchus sp. were also detected in various locations, however, their densities were very low. Root-knot nematode females were collected from tomato roots inoculated with the potato field soils for PCR-RFLP identification. The females from Cheju, Milyang, and Goryung showed PCR products of 500 bp. And the Dra I restriction enzyme digestions showing 290 bp and 230 bp fragments confirmed their identity as Meloidogyne hapla.

심비디움에 발생하는 해충의 종류

조명래,전성욱,강택준,김형환,안승준,양창열,Cho, Myoung Rae,Jeon, Sung-Wook,Kang, Taek Joon,Kim, Hyung Hwan,Ahn, Seung-Joon,Yang, Chang Yeol 한국응용곤충학회 2013 한국응용곤충학회지 Vol.52 No.4

수출용 심비디움에 발생하는 해충의 종류와 해충관리 현황을 알아보고자 국내 주요 재배단지 10개 지역 45농가를 대상으로 조사를 실시하였다. 농가에서 채집된 해충의 종류는 응애류(20농가), 깍지벌레류(6농가), 민달팽이류(6농가), 총채벌레류(8농가), 진딧물류(5농가) 노린재류(1농가) 등이었다. 채집한 해충은 현미경을 이용하여 동정한 결과 점박이응애(Tetranychus urticae Koch), 대만총채벌레(Frankliniella intonsa Trybom), 난초핀깍지벌레(Pinnaspis aspidistrae Signoret), 민달팽이(Incilaria confusa Cockarel), 썩덩나무노린재(Halyomorpha brevis Walker), 복숭아혹진딧물(Myzus persicae Sulzer), 목화진딧물(Aphis gossypii Glover), 무화과깍지벌레(Coccus hesperidum Linnaeus)와 소형달팽이류 1종으로 나타났다. 총채벌레 산란피해로 의심되는 증상의 정확한 동정을 위해 rDNA의 ITS2 부위를 PCR-RFLP 한 결과 아까시총채벌레(Thrips flavus Schrank)와 파총채벌레(Thrips tabaci Lindeman)로 확인되었다. 따라서, 수출 심비디움을 재배하는 농가에서는 이러한 해충들에 대한 종합적인 방제대책을 수립하여 실천하는 것이 필요할 것으로 생각된다. A survey of pest occurrence and status of farmer's pest management was conducted at 45 cymbidium farms in 10 major cultivation areas in Korea. The pest species collected from the cymbidium farms were identified as follows: Tetranychus urticae Koch, Frankliniella intonsa Trybom, Pinnaspis aspidistrae Signoret, Incilaria confusa Cockarel, Halyomorpha brevis Walker, Myzus persicae S$\ddot{u}$lzer, and Aphis gossypii Glover, Coccus hesperidum Linnaeus, Thrips flavus Schrank, and Thrips tabaci Lindeman. The two-spotted spider mite, T. urticae, was the key pest in cymbidium production, occurring on 45 farms, followed by scales (20 farms), slugs (6), thrips (8), aphids (5), and stinkbug (1). PCR-RFLP of the rDNA ITS2 region revealed that two thrips species, Thrips flavus Schrank and Thrips tabaci Lindeman, occur on cymbidium farms. Therefore, it is necessary for the cymbidium farmers to establish an integrated pest management system to meet quarantine standards.

심비디움에 발생하는 선충 조사 및 줄기선충류(Ditylenchus sp.)에 대한 약제방제 효과

조명래,강택준,김형환,안승준,전성욱,전재용,김영호,Cho, Myoung-Rae,Kang, Taek-Joon,Kim, Hyung-Hwan,Ahn, Seung-Joon,Jeon, Sung-Uk,Chun, Jae-Yong,Kim, Young-Ho 한국응용곤충학회 2012 한국응용곤충학회지 Vol.51 No.2

수출 심비디움 재배 시 검역상 문제되는 선충의 발생 여부를 알아보고자 전국의 심비디움 재배단지에서 수집한 1-3년생 심비디움 근권부에 발생하는 선충의 종류를 조사하였다. 1-3년생 심비디움 근권부에서 관찰된 식물기생성 선충은 Ditylenchus속으로 밀도는 배지 200 cc 당 각각 4.7, 43.7, 49.7마리였다. 자유생활성 선충류인 Dorylaimid 선충의 밀도는 배지 200 cc당 각각 35.3, 70.5, 155.8마리였다. 시흥, 안산, 김포의 3년생 심비디움에서는 Aphelenchus속, Aphelenchoides속 선충이 관찰되었으나 화분 당 40마리 이하로 밀도가 낮았으며 Dorylaimid가 화분 당 56-824마리로 나타났다. 선충방제용 약제선발을 위해 Emamectin benzoate EC, Fosthiazate SL, Cadusafos CS 등 3종의 약제로 안산과 김포의 2개 농가에서 방제효과를 시험하였다. Emamectin benzoate EC는 2개소 시험에서 75.7, 89.5%의 방제가를 보였으며 Fosthiazate SL, Cadusafos CS는 각각 27.2, 65.3% 및 30.1, 90.5%의 방제가를 나타내었다. Surveys were conducted on the occurrence of nematodes in the root systems of 1-3-year old Cymbidium hybida Swartz cultivated for export in Korea. The most frequently detected plant-parasitic nematode was Ditylenchus sp. with 4.7, 43.7, and 49.7/200 cc growth medium in 1, 2, and 3 year-old cymbidiums, respectively. And the densities of non-parasitic nematodes, dorylaimids, were 35.3, 70.5, and 155.8/200 cc growth medium in 1, 2, and 3-year-old cymbidiums, respectively. Three-year-old cymbidiums collected from Siheung, Ansan, and Gimpo had low densities of Aphelenchus sp. and Aphelenchoides sp. with under 40 individuals/pot, and the dorylaimid densities were 56-824/pot. To evaluate the effects of nematicides on Ditylenchus sp. in cymbidium, Emamectin benzoate EC, Fosthiazate SL, and Cadusafos CS were tested at two farms in Ansan and Gimpo. Emamectin benzoate EC showed control effects of 75.7 and 89.5%, whereas Fosthiazate SL and Cadusafos CS showed 27.2 and 65.3% and 30.1 and 90.5% control effects in the tests.

유우의 질탈교정술의 일예

조명래,Cho Myoung-Rae 한국임상수의학회 1992 한국임상수의학회지 Vol.9 No.1

Four retention sutures were used to correct the vaginal prolapse in a dairy cow. The methods used were two modified Caslick operation of vulva using absorbable(catgut chromic) and nonabsorbable(supramid) materials, and a modified mattress closure and a modified Buhner method of vulva using trocar and wire insulated by vinyl. The lasting periods for retention of bovine vaginal prolapse by the suture methods described above, were 12 days, 49 days, 39 days and 62 days respectively.

국내 산림식물 11종의 항산화 활성

조명래,이종석,이사라,손연경,배창환,여주홍,이해석,마진경,이옥환,김종예,Cho, MyoungLae,Lee, Jong Seok,Lee, Sarah,Son, Youn Kyoung,Bae, Chang-Hwan,Yeo, Joohong,Lee, Hae-Sock,Ma, Jin-Gyeong,Lee, Ok-Hwan,Kim, Jong-Yae 한국식품영양학회 2015 韓國食品營養學會誌 Vol.28 No.6

This study aimed to investigate antioxidant activities from 11 forest plants, and determine their total phenolics, flavonoids and proantocyanidins contents. In addition, the antioxidant activities were correlated with antioxidant compounds. Among the samples, Cornus officinalis, Castanea crenata, Lindera erythrocarpa, Carpinus laxiflora and Pourthiaea villosa showed significantly higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) ($IC_{50}=21.12{\sim}28.93{\mu}g/mL$) and 2,2'-azino-bis(3-ethylbenzothia zoline-6-sulfonic acid) (ABTS) ($IC_{50}=28.18{\sim}52.55{\mu}g/mL$) radical scavenging ability with reducing power ($IC_{50}=59.91{\sim}93.64{\mu}g/mL$) than other plants; and C. crenata, L. erythrocarpa and Rubus coreanus showed strong nitric oxide (NO) inhibition activity (${\geq}60%$). In addition, L. erythrocarpa, C. laxiflora and P. villosa showed higher oxygen radical absorbance capacity (ORAC) values (${\geq}1,100{\mu}M$ TE/g sample) than other samples. High total phenolic contents were observed in C. crenata (429.11 mg GAE/g), L. erythrocarpa (437.11 mg GAE/g), C. laxiflora (408.67 mg GAE/g) and P. villosa (404.11 mg GAE/g). The DPPH and ABTS radical scavenging activity with reducing power were significantly correlated with total phenolic contents ($R^2=0.71{\sim}0.79$), but total phenolic contents were not correlated with NO inhibition and ORAC ($R^2=0.35{\sim}0.43$). Therefore, these results suggested that C. officinalis, C. crenata, L. erythrocarpa, C. laxiflora and P. villosa are potential natural antioxidative candidate ingredients.

Video-on-Demand 서비스망의 자원 할당 문제를 위한 동적계획법

조명래,Cho, Myeong-Rai 한국정보처리학회 2000 정보처리논문지 Vol.7 No.10

B-ISDN(Broadband-Integrated Services Digital Network)의 출현으로 주문형 비디오(Video On Demand: VOD) 서비스는 차세대 유망한 영상 정보 서비스로 관심의 대상이 되고 있으며, 대화성의 정도에 따라 크게 IVOD(Interactive VOD)와 NVOD(Near VOD) 서비스로 분류된다. 다계층 구조 서비스망에서 VOD 서비스를 하기 위해서는 여러 자원이 필요하겠지만 본 연구에서는 비디오 서버, 프로그램, 그리고 저장창고로 두고, 프로그램 전송 비용, 프로그램 저장 비용, 그리고 비디오 서버 설치 비용을 최소화하는 자원 최적화 문제를 두 가지 형태로 나누어 그 해결 방법을 제시하였다. 첫째는 VOD를 IVOD로만 서비스 하는 경우 서비스 운용 비용을 최소화하는 비디오 서버의 위치 선정과 설치된 비디오 서버에 저장할 프로그램 종류와 그 양을 결정하는 문제로, 이를 효과적으로 해결하기 위해 동적계획법을 이용하였다. 둘째는 VOD서비스를 보다 경제적으로 제공하기 위해, VOD를 IVOD와 NVOD의 혼합 서비스로 제공하는 경우 서비스 운용 비용을 최소화 하는 NVOD프로그램의 종류와 그 양 그리고 그 때의 IVOD서비스를 위한 비디오 서버의 위치 선정 및 설치된 비디오 서버에 저장 시킬 프로그램 종류와 그 양을 결정하는 문제로 동적계획법을 확정하여 제시하였다. It is strongly beliefed that Video on Demand(VOD) will become one of the most promising services in Broadband Integrated Services Digital Network(B-ISDN) for the nest generation VOD service can be classified into two types of services 'Near VOD(NVOD) and Interactive VOD(IVOD). For both services, some video servers should be installed at some nodes(especially, at the root node for NVOD service) in the tree structued VOD network,so that each node with video server stores video programs and distribules stored programs to customers. We consider thre kinds of cost.a program transmission cost, a program storage cost, and a video server installation cost. There exists a trade-off relationship among those three costs according to locationsl of video servers and the kinds of programs stord at each video server. Given a tree structured VOD network, and the total number of programs being served in the network, the resource allocation problem in a VOD network providing both IVOD and NVOD services is to determine where to install video servers for IVOD service, which and how many programs should be stored at each video server for both IVOD and NVOD services, so as to minunize the total cost which is the sum of three costs for both IVOD and NVOD services. In this paper we develop an efficient cynamic programming algorithm for solving the problem. We also implement the algorithm based on a service policy assumed in this paper.