제초제저항성 GM 잔디에서 발현된 PAT 단백질의 알레르겐 유발 가능성 및 독성 평가

정혜린,선현진,강지남,강홍규,이효연,Jeong, Hye-Rin,Sun, Hyeon-Jin,Kang, Ji-Nam,Kang, Hong-Gyu,Lee, Hyo-Yeon 한국식물생명공학회 2020 JOURNAL OF PLANT BIOTECHNOLOGY Vol.47 No.4

본 연구는 제초제저항성 GM 들잔디에서 발현된 PAT 단백질의 잠재적인 알레르기성 및 독성을 평가하기 위해 수행되었다. PAT 단백질의 in silico 분석에서 PAT 단백질은 알려진 알레르겐 또는 독성 단백질과 유사성을 보이지 않았다. PAT 단백질은 80개의 아미노산으로 이루어진 절편에 걸쳐 기지의 알레르겐과 35% 미만의 아미노산 서열 상동성을 보였고, 기지의 알레르겐과 연속한 8개의 아미노산에서 일치하는 서열도 확인되지 않았다. 또한 제초제저항성 GM 들잔디에서 발현된 PAT 단백질은 펩신 존재 하의 인공위액에서 매우 빠르게 분해되었고, GM 들잔디에서 발현된 PAT 단백질은 번역 후 수식(glycosylation)도 일어나지 않았음이 확인되었다. 경구투여 독성 시험에서는 체중 1 kg 당 4,000 mg 을 투여한 실험구에서도 PAT 단백질 투여 후 마우스에서 사망이나 독성 영향은 관찰되지 않았다. 이들 결과는 제초제저항성 GM 들잔디에서 발현된 PAT 단백질이 잠재적 알레르겐이나 독소로 작용할 가능성이 없음을 시사한다. This study aimed to evaluate the potential allergenicity and oral toxicity of the phosphinothricin acetyltransferase (PAT) protein expressed in Zoysia japonica, a herbicide-tolerant genetically modified (GM) zoysiagrass. In silico analysis of PAT showed no similarities with any known allergenic or toxic proteins, with <35% amino acid sequence homology with known allergens across a length of 80 amino acids and no continuous eight amino acid identity with known allergens. The PAT protein expressed in Z. japonica degraded very rapidly in the simulated gastric fluid in the presence of pepsin, and, no glycosylation of PAT was observed. The oral toxicity test revealed no mortality or toxic effect in mice following PAT administration at 4,000 mg/kg body weight. Our findings indicate that the PAT protein expressed in Zoysia japonica does not exhibit allergenic or toxic properties.

유튜브 패션 크리에이터 채널의 콘텐츠 분석 연구

정혜린(Hye Rin Jeong),박길순(Kil Soon Park),김한나(Hanna Kim) 한국생활과학회 2019 한국생활과학회 학술대회 Vol.2019 No.5

미니피그에서 자가치아뼈 이식의 골형성 효과에 대한 연구

정혜린(Hye Rin Jeong),황주홍(Ju Hong Hwang),이정근(Jeong Keun Lee) 대한구강악안면외과학회 2011 대한구강악안면외과학회지 Vol.37 No.5

Introduction: This study examined the effect of autogenous tooth bone used as a graft material for bone regeneration in an artificial bony defect of minipigs. Materials and Methods: Four healthy minipigs, weighing approximately 35-40 kg, were used. Four standardized artificial two-walled bony defects, 5 mm in length and depth, were made on the bilateral partial edentulous alveolar ridge on the mandible of minipigs, and autogenous tooth bone was augmented in the right side as the experimental group. On the other hand, only alloplastic bone graft material HA was grafted with the same size and manner in the left side as the control group. All minipigs were sacrificed at 4 weeks after a bone graft and evaluated histologically by Haematoxylin-eosin staining. The specimens were also evaluated semi-quantitatively via a histomorphometric study. The percentage of new bone over the total area was evaluated using digital software for an area calculation. Results: All specimens were available but one in the left side (control group) and two in the right side (experimental group) were missing during specimen preparation. The amount of bone formation and remodeling were higher in all experimental groups than the control. The mean percentage area for new bone in the experimental and control groups was 43.74±11.96% and 30.79±2.93%, respectively. Conclusion: Autogenous tooth bone is a good alternative to autogenous bone with the possible clinical feasibility of an autogenous tooth bone graft in the reconstruction of bony defects.

인스타그램에 나타난 동묘 구제시장 패션에 관한 연구

정혜린(Hye rin Jeong),김한나(Han na Kim) 한국생활과학회 2018 한국생활과학회 학술대회 Vol.2018 No.11

금은화 에틸 아세테이트 분획물의 항산화 활성 및 H₂O₂로 유도된 산화적 스트레스에 대한 신경세포 보호 효과

정혜린(Hye Rin Jeong),김종민(Jong Min Kim),강진용(Jin Yong Kang),문종현(Jong Hyun Moon),김민지(Min Ji Kim),이효림(Hyo Lim Lee),고민지(Min Ji Go),구수연(Su Yeon Gu),김현진(Hyun Jin Kim),허호진(Ho Jin Heo) 한국식품저장유통학회 2021 한국식품저장유통학회지 Vol.28 No.7

비스포스포네이트로 인한 하악 및 상악골에 발생한 골괴사에 대한 임상적 연구

정혜린(Hye-Rin Joeng),김태완(Tae-Wan Kim),이정근(Jeong- Keun Lee),송승일(Seung-Il Song) 대한구강악안면외과학회 2009 대한구강악안면외과학회지 Vol.35 No.5

Bisphosphonates are compounds widely used in the treatment of various metabolic and malignant bone disease. Recently, an association between bisphosphonate use and a rare dental condition termed osteonecrosis of the jaw(ONJ) has been reported. Bisphosphonate-related osteonecrosis of the jaw(BRONJ) is rare, but serious, side effect of bisphosphonate therapy in affected patients. It is characterized by poor wound healing and spontaneous intra-oral soft tissue break down, which lead to exposure of necrotic maxillary and mandibular bone. We reviewed 11 patients of BRONJ visited Ajou University Hospital Dental clinic from May 2007 to November 2008. The management of the patients included cessation of bisphosphonate therapy and various surgical restorative procedures and conservative care there after. Aggressive debridement is contraindicated. A new complication of bisphosphonate therapy administration, osteonecrosis of jaws, seems to be developing. The improved results after cessation of the medication should make clinicians reconsider the merits of the rampant use of bisphosphonates, while further investigation is needed to completely elucidate this complication.

Outfracture osteotomy sinus graft technique의 유용성에 관한 임상적 고찰

송승일,정혜린,김형모,이정근,Song, Seung-Il,Jeong, Hye-Rin,Kim, Hyung-Mo,Lee, Jeong-Keun 대한구강악안면외과학회 2009 대한구강악안면외과학회지 Vol.35 No.5

Purpose: The purpose of this study was to evaluate the feasibility of the outfracture osteotomy sinus graft technique with the evaluation of 5-year survival rate of the implants placed in the atrophic edentulous posterior maxillary area. Materials and methods: One hundred and thirteen cases of 96 patients who visited our center from Aug 2004 to July 2009 and were diagnosed as atrophic edentulous maxillary alveolar ridge, were selected and underwent augmentation sinus surgery with outfracture osteotomy technique. Feasibility of the outfracture osteotomy technique was investigated with clinical and radiographic evaluation to assess the survival rate of the total dental implants in augmentation sinus surgery of this new kind. Total fixture number available in follow-up period was 179, in which the lost 10 patients were excluded out of 96 patients. Results: Five-year cumulative survival rate was 97.2% with 5 failures of total 179 fixtures. The average follow-up period was 29 and a half months, with the minimum and maximum follow-up periods of 4 months 21 days and 59 months 14 days, respectively. Conclusion: Traditional infracture technique is a popular method for an augmentation sinus surgery. The authors modified this classical method by outfracturing and readapting the bony window after sinus graft, with excellent treatment results evidenced by high survival rate of 97.2% (174 out of 179 fixtures), which proves the feasibility of the newly-designed outfracture osteotomy sinus graft technique.

한국 전통 상복과 현대의 상복 변화에 관한 연구

소황옥(Hwang-Oak Soh),정혜린(Hye-Rin Jeong) 한복문화학회 2004 韓服文化 Vol.7 No.3

Today it's not difficult to come across a good number of valuable cultural assets being made light of or ignored, which is never considered trivial. Among the many items of cultural heritage, it's also important to preserve and pass down the traditional Korean mourning garments. The mourning ceremonies have been regarded as the most grieving and solemn element of passage rites, being extremely complex and refined in terms of procedures. Our ancestors under the influence of Confucianism placed the most importance on the ceremonies of mourning among the four major ceremonies of one's life, which included the coming-of-age, wedding, and religious ones in addition to the mourning ceremonies. They were thorough with following the complicated and difficult procedures of the biggest ceremonies and reflected their sadness and grief in their mourning garments that they wore for a certain period of time, believing that they were fulfilling the filial duty by doing all those requirements. Entering the modern era, the Korean society started to simplify the related ceremonies simply mistaking them as the origin of all the formalism and to replace the traditional mourning ceremonies with the new simple process of handling the dead body based on indifference. <br/> Even though the western culture has penetrated deep into the Korean society, where the traditional order has gone through huge revolutionary steps and the Confucian ideas have their social roles reduced to a great degree, the potential impacts of Confucianism cannot be underestimated with the ideas engraved in the consciousness of the Koreans. <br/> Thus this study intended to investigate the traditional Korean mourning garments and examine what kind of changes were introduced to the mourning garments in the modern times. In addition, the problems with the modern changes, if any, were identified, which were followed by solutions suggested.

월인석보 권20 훼손 부분의 복원과 이용

( Kawasaki Keigo ),( Sugiyama Yutaka ),정혜린 ( Jeong Hye-rin ) 국어학회 2013 국어학 Vol.67 No.-

≪月印釋譜≫ 卷20의 현존본은, 안타깝게도 <太子須大拏經> 해당 부분이 68장부터 83장까지 15장에 걸쳐 지면 하단 부분을 중심으로 심하게 훼손되어 있어 자료로서 이용하기가 어려웠다. 그러나 사실 이는 어느 정도 복원이 가능하다. 즉, 훼손 부분에 몇 글자가 들어가는지를 정확히 세어, 底經인 <太子須大拏經>을 자세히 검토하되 ≪釋譜詳節≫, ≪月印釋譜≫에서의 전반적인 底經 번역 양상도 함께 고려하면서 우리가 현재 가지고 있는 중세한국어에 대한 모든 지식을 동원하면, 상당 부분에 대해 어느 정도 객관성이 있는 복원 작업을 할 수가 있는 것이다. 본고는 그러한 작업을 시도하여, 나아가 그 결과물 중에서 한국어사적으로 이용가치가 높은 정보를 구체적으로 지적함으로써 이 복원 작업의 학술적 중요성을 강조하고자 한다. It is unfortunate for us that the only available book of the volume 20 of Worin-seokbo is seriously damaged from the page 69 to 83 mainly in the lower part of it, so we find it difficult to read the part of the Middle Korean translation of original Chinese sutra Taizi Xudana Jing (太子須大拏經, Prince Sudana Sutra), or to utilize it as a historical language text. It is, however, not impossible for us to restore the original text to some extent: At first we precisely count the number of letters to be restored, then translate the corresponding letters of Taizi Xudana Jing into Middle Korean with all the our grammatical knowledge available, carefully contrasting with general style and patterns of translation in Seokbosangjeol and Worin-seokbo. We will not only try to restore the original text as much as possible, but also try to find out some newly available historical linguistic facts from it so we can illustrate the significance of this work.

일개 대학 간호학생들의 공감능력과 문화적 역량과의 관계

조미경 ( Mi Kyoung Cho ),신이나 ( I Na Shin ),이예진 ( Yea Jin Lee ),이지효 ( Ji Hyo Lee ),장은혜 ( Eun Hye Jang ),정혜린 ( Hye Rin Jeong ),차경민 ( Kyung Min Cha ) 서울대학교 간호과학연구소 2015 간호학의 지평 Vol.12 No.2

Purpose: This study aimed to identify the relationship between empathic ability and cultural competency in university nursing students. Methods: This correlational study involved 139 second- and third-year nursing students. They completed a 3-part questionnaire comprising items assessing general characteristics (9), empathic ability (30), and cultural competency (27). Results: The mean scores for empathy and cultural competency were 106.0 and 78.7, respectively. Empathy did not differ significantly by general characteristics. However, cultural competency significantly differed by foreign language skill. Additionally, a significant correlation was found between the nursing students’ empathic ability and cultural competency. Conclusion: Nursing students’ empathic ability and cultural competency need to be enhanced to ensure the provision of high-quality nursing services to foreign patients.