유근피 추출물의 3T3-L1지방전구세포의 분화 억제 효능에 관한 연구

정현영(Hyun Young Jeong),진수정(Soojung Jin),남수완(Soo Wan Nam),현숙경(Sook Kyung Hyun),김성구(Sung Gu Kim),김병우(Byung Woo Kim),권현주(Hyun Ju Kwon) 한국생명과학회 2014 생명과학회지 Vol.24 No.2

유근피(Cortex ulmi pumilae)는 항산화, 항염증, 항균, 항암 등 다양한 생리활성이 보고되어 있으며, 뛰어난 소염 효과로 예로부터 약재로 사용되어 왔다. 본 연구에서는 3T3-L1 지방전구세포를 이용하여 유근피 추출물의 지방세포 분화에 미치는 영향과 그 기전을 검토하였다. 유근피 추출물을 분화유도제와 동시에 처리한 결과, 3T3-L1 지방 전구세포의 세포질 내 triglyceride 생성을 농도의존적으로 감소시켰으며, 지방세포분화 관련 전사인자인 C/EBP α, C/EBPβ 및 PPARγ의 발현을 유의적으로 저하시켰다. 다음으로 유근피 추출물을 지방세포 분화 유도 단계별로 처리한 결과, 분화유도제와 동시에 처리한 경우 즉 분화 초기에 처리한 경우가 가장 효과적인 지방세포 분화 억제 효능을 보였으며, 이 때 분화초기에 발현되는 C/EBPβ의 발현량은 현저하게 감소되었다. 또한 유근피 추출물에 의해 mitotic clonal expansion 단계의 세포증식이 농도의존적으로 억제되는 것을 확인하였으며, 세포주기분석을 통해 G1 arrest를 유발함으로써 증식이 억제 된다는 것을 확인하였다. 또한, G1 arrest는 G1/S 전이 관련 단백질인 p21의 발현 증가, cyclin E의 발현 감소 및 Rb의 인산화 감소에 의한 것임을 확인하였다. 이러한 연구결과들로부터 유근피 추출물은 지방세포 분화 초기 단계에 작용하여 지방세포 분화 관련 전사인자의 발현을 억제하고 mitotic clonal expansion을 저해하여, 지방전구세포의 지방세포로의 분화를 억제하는 항비만 효능을 가지는 천연 소재임을 확인하였으며, 따라서 본 연구는 향후 이를 활용한 지속적인 연구를 위한 기초자료로 그 가치가 매우 높을 것이라 생각된다. Cortex ulmi pumilae, the cortex of Ulmus davidiana var. japonica, has been used in traditional folk medicine for its anti-inflammatory effect. Although its various bioactivities such as anti-inflammatory, anti-microbial, and anti-cancer, have been reported, the anti-adipogenic activity of cortex ulmi pumilae remains unclarified. In the present study, we investigated the effect of cortex ulmi pumilae extract on adipocyte differentiation in 3T3-L1 preadipocytes. Treatment with cortex ulmi pumilae extract significantly reduced the formation of lipid droplets and triglyceride content in a dose-dependent manner; this is associated with an inhibition of the adipogenic transcription factors, CCAAT/enhancer binding protein α (C/EBPα), C/EBPβ, and peroxisome proliferator-activated receptor γ (PPARγ). In addition, cortex ulmi pumilae extract treatment during the early stage of adipogenesis showed more efficient anti-adipogenic activity than treatment during other stages of adipogenesis. Cortex ulmi pumilae extract also inhibited cell proliferation and induced G1 arrest of 3T3-L1 cells in the early stage of adipogenesis. This was associated with upregulated expression of Cdk inhibitor p21 and downregulated expression of cyclin E and phospho-Rb, indicating that cortex ulmi pumilae extract blocks mitotic clonal expansion by cell cycle regulation. Taken together, these results suggest that cortex ulmi pumilae extract possesses anti-adipogenic activity through the inhibition of adipocyte differentiation by blocking mitotic clonal expansion.

중학교 특수학급 교사의 관찰법과 포트폴리오 평가 실태

정현영 ( Hyun Young Jeong ),정은희 ( Eun Hee Jeong ) 한국특수아동학회 2010 특수아동교육연구 Vol.12 No.1

효과적인 피부 질감 표현을 위한 메이크업 도구 사용에 관한 연구 - 파운데이션 브러시를 중심으로 -

정현영 ( Hyun-young Jeong ),이솔 ( Sol Lee ),이정민 ( Jung-min Lee ) 한국미용예술경영학회 2011 미용예술경영연구 Vol.5 No.1

Since the middle of 2000, due to the increasing level of interest towards such global issues as well-being and health, the so-called baby-face make-up has become a hot topic. As a result, the tone-based make-up pattern has been changed to the one based on the skin expression. In terms of make-up, the skin expression is not a basic stage for the tone-based make-up any more. It is currently regarded as the most important stage. Therefore, the make-up manufacturers are showing a high level of interest in the development of make-up products and tools which are suitable for various kinds of skin expression related to the baby-face trend. In particular, the specialized foundation brush makes it possible to apply foundation lightly on one's face and leaves shine behind. Therefore, such a tool is suitable for the moist and natural skin expression. The objective of this study is to analyze the effective use of specialized foundation brushes with various shapes, consider the effective method of skin expression and provide related information. As a study method, in order to exclusively analyze the effective brushes for individual foundation products, three foundation brushes and four foundation products with the highest level of preference in the market have been chosen for the in -depth interview and investigation with the group consisting of seven specialists. The contents which have been revealed through the in -group interview have been organized. As a result, it has been found that Foundation Brush A made of techron with round edges and a high level of elasticity is suitable for the use of Liquid Foundation A and Liquid Foundation B with a high level of moisture. Even if it looks similar to Foundation Brush A and makes it possible to adjust the amount of moisture which it absorbs, the soft-typed Foundation Brush B made of techron with the texture of easy cracking based on too much moisture is the most suitable for the use of the general-typed Liquid Foundation B which is not too watery. Foundation Brush C made of techron and natural materials with round edges shows a flat shape on the top. Since it makes it difficult to adjust the amount of foundation and shows a covering power when it is applied, it is the most suitable for the use of Cream Foundation B which is the solid kind of foundation with a high amount of oil. This study limits the subjects of the analysis to the group of make-up specialists. As a result, it is difficu1t to generalize the results. It is necessary to increase the sample subjects of the analysis and carry out a study on the utilization of various make-up tools other than the foundation brush.

금앵자 에탄올 추출물에 의한 3T3-L1 지방세포의 분화억제 효과와 그 메커니즘 규명

정현영 ( Hyun Young Jeong ),정인교 ( In Kyo Jeong ),남소연 ( So Yeon Nam ),윤희정 ( Hee Jung Yun ),김병우 ( Byung Woo Kim ),권현주 ( Hyun Ju Kwon ) 한국미생물생명공학회(구 한국산업미생물학회) 2016 한국미생물·생명공학회지 Vol.44 No.1

비만은 체내 지방이 과도하게 축적되어 일어나는 현상으로, 당뇨, 고혈압, 심혈관 질환 및 암과 같은 질병의 원인이 된다. 본 연구는 RLE에 의해 지방전구세포에서 지방세포로 분화 시, 세포 내 축적되는 Triglyceride 저해 및 발현되는 전사인자들의 발현양상에 미치는 영향에 대하여 조사하였다. 그 결과, RLE는 Oil Red O 염색에서 세포 내 triglyceride의 축적을 농도 의존적으로 억제하였다. 또한 CCAAT/enhancer binding protein(C/EBP) α, β와 peroxisome proliferator activated receptor γ(PPARγ)과 같은 지방세포 분화 관련 전사인자들의 발현을 억제하였다. RLE는 clonal expansion 단계의 지방세포를 G1기에서 세포 주기를 정지시켜 세포의 증식을 억제하였으며, RLE 처리에 의해 p21의 증가, Cyclin E, Cdk2, Phospho-Rb의 발현 저해 등 G1 arrest 관련 단백질의 발현 변화가 유도되었다. 따라서, RLE는 분화 관련 전사인자들의 발현을 조절하고 지방세포 분화 초기에 G1기의 세포 주기 정지를 억제함으로써 지방전구세포에서 지방세포로의 분화를 억제한다고 사료된다. Obesity is caused by excess accumulation of body fat and contributes to various pathological disorders such as diabetes, hypertension, cardiovascular disease, and cancer. In this study, we investigated the effect of a 30% ethanol extract of Fructus Rosae laevigata (RLE) on adipogenesis in 3T3-L1 adipocytes, measured by triglyceride accumulation and expression of adipogenesis-related transcription factors during differentiation of pre-adipocytes into adipocytes. RLE decreased the intracellular triglyceride contents (assessed by Oil Red-O staining) in a dose-dependent manner. It also downregulated the expression of adipogenic transcription factors and inhibited cell proliferation during the mitotic clonal expansion phase of adipocyte differentiation by inducing G1 phase arrest. We investigated the alterations in the levels of G1 phase arrest-related proteins. The expression of p21 protein significantly increased, while the levels of Cyclin E, Cdk2, and phospho-Rb decreased in a dose-dependent manner in 3T3-L1 cells treated with RLE. These results suggest that RLE inhibits the differentiation of 3T3-L1 adipocytes by suppressing the expression of adipogenic transcription factors and inducing G1 phase arrest in the early stages of adipocyte differentiation.

Inhibition of Adipocyte Differentiation through G1 Arrest by Extract of Sophora tonkinensis Gapnep in 3T3-L1 Preadipocytes

Hyun-Young Jeong(정현영),Sook-Kyung Hyun(현숙경),Yung Hyun Choi(최영현),Byung-Woo Kim(김병우),Hyun-Ju Kwon(권현주) 한국생명과학회 2011 생명과학회지 Vol.21 No.9

산두근(Sophora tonkinensis Gapnep)은 예로부터 동양지역에서 전통적인 약용식물로 사용되어 왔다. 본 연구에서는 3T3-L1 지방전구세포의 성숙지방세포로의 분화와 세포 내 지방생성에 대한 산두근 메탄올 추출물((STME)의 효과와 메커니즘에 대해 조사하였다. STME를 0-200 μg/ml의 농도로 처리한 다음, Oil Red O 염색으로 세포 내 축적되는 지방구와 지질의 양을 측정한 결과 농도의존적으로 크게 감소됨을 확인하였으며 3T3-L1 지방전구세포의 분화와 관련된 단백질의 발현의 변화를 조사하였다. 지방세포의 특이적 marker인 peroxisome proliferator-activated receptor γ (PPARγ), cytidine-cytidine-adenosine-adenosine-thymine (CCAAT)/enhancerbinding proteins α, β (C/EBPα, C/EBPβ) 그리고 sterol regulatory element binding protein (SREBP)의 발현이 STME를 처리하였을 때 현저하게 저해됨을 확인하였다. 세포주기의 변화를 분석한 결과 STME는 지방세포 분화 초기 단계인 mitotic clonal expansion 단계에서 G1기로 세포주기를 정지시켰다. 더불어 G1 arrest와 관련된 단백질의 변화를 조사 한 결과, 3T3-L1 세포에 STME를 처리하였을 때 p21의 발현량이 확연하게 증가하였으며, Cdk2, E2F-1 그리고 phosphor-Rb의 발현량은 농도의존적으로 감소하였다. 이러한 결과들에 의하여 STME은 메탄올 추출물임에도 불구하고 3T3-L1 지방전구세포가 성숙지방세포로 분화할 때 G1 arrest를 통하여 지방세포 분화를 억제하며 관련 유전자의 발현 억제도 확연하게 확인할 수 있었으며, 이러한 결과는 항 비만 천연물 소재 탐구의 기초자료로 유용하게 쓰일 것으로 사료된다. Sophora tonkinensis Gapnep has been used as a traditional herbal medicine in oriental regions since ancient times. In this study, the effect and mechanism of the MeOH extract of Sophora tonkinensis Gapnep (STME) on adipocite differentiation and adipogenesis in 3T3-L1 preadipocites were investigated. Treatment with STME in the concentration range of 0-200 μg/ml significantly inhibited the differentiation of 3T3-L1 preadipocites in a dose-dependent manner, as determined by a decrease in intracellular lipid droplets and lipid contents measured by Oil Red O staining. In association with the inhibitory effect of lipid accumulation, the expressions of the proteins concerned with adipogenesis in 3T3-L1 preadipocites were also investigated. Treatment with STME reduced the expressions of peroxisome proliferator-activated receptor γ (PPARγ), cytidine-cytidine-adenosine-adenosine-thymine (CCAAT)/enhancer-binding proteins α and β (C/EBPα and C/EBPβ) and sterol regulatory element binding protein (SREBP), which are adipocyte specific markers. In flow cytometry analysis, the inhibitory effect of differentiation was caused by G1 arrest and following mitotic clonal expansion cease. Therefore, we also investigated the alteration of G1 phase arrest-related proteins. As a result, the expression of p21 protein was significantly increased, while the expressions of Cdk2, E2F-1 and phospho-Rb were reduced in a dose-dependent manner in STME treated 3T3-L1 cells. According to these results, STME might inhibit differentiation through G1 arrest in 3T3-L1 preadipocytes adipogenesis, and further studies, which are in progress, have to be completed to identify the active compounds.

팔각회향 dichloromethane 분획물에 의한 지방세포 분화 억제 효과

정현영(Hyun Young Jeong),정인교(In Kyo Jeong),김남주(Nam Ju Kim),윤희정(Hee Jung Yun),박정하(Jung Ha Park),김병우(Byung Woo Kim),권현주(Hyun Ju Kwon) 한국생명과학회 2019 생명과학회지 Vol.29 No.4

비만은 에너지의 불균형으로 인하여 체내 지방조직에 지방이 축적되는 대사성질환으로 심혈관계 질환, 고혈압, 2형 당뇨, 고지혈증 및 각종 암의 발생 빈도를 증가시키는 요인이다. 지방의 축적은 지방전구세포가 지방세포로 분화하는 과정을 의미하는 adipogenesis라는 과정을 거쳐서 일어난다. 지방세포로의 분화는 다양한 호르몬과 전사인자들의 상호작용에 의해서 일어난다. 본 연구에서는 팔각회향이 항비만 소재로 활용 가능한지 확인하기 위해, 팔각회향 물 추출물을 분획하여 지방축적 억제 활성이 좋은 dichloromethane층을 선정하였다. 3T3-L1 지방전구세포가 성숙한 지방세포로 분화할 때 팔각회향 dichloromethane 층이 어떠한 기전으로 분화를 조절하는지 확인한 결과, 지방세포 분화에서 중요한 전사인자인 C/EBP family, PPARγ의 발현이 억제되었고, 지방세포 최종 분화마커로 알려져 있는 FAS 및 LPL의 발현 또한 감소되었다. 또한 G1기에서의 세포주기 정지를 통해 지방세포 분화단계에서 필수적인 mitotic clonal expansion 단계를 억제한다는 결과를 얻었다. 이러한 연구 결과는 팔각회향이 항비만 효과를 가지는 천연물 소재로의 활용가능성을 보여주는 기초 자료가 될 것으로 사료된다. Fat accumulation in adipocytes occurs through the process of adipogenesis in which preadipocytes differentiate into adipocytes. Obesity is a metabolic disorder caused by excessive accumulation of fat in the body, which increases the incidence of cardiovascular diseases, hypertension, type 2 diabetes, hyperlipidemia, and various cancers. Recently, inhibition of adipocyte differentiation was shown to be a potential antiobesity strategy. In this study, the inhibitory effect of dichloromethane fractions from Illicium verum Hooker fil. water extract on the differentiation of 3T3-L1 preadipocytes to adipocytes was investigated. Dichloromethane fractions from I. verum Hooker fil. significantly inhibited adipocyte differentiation when applied during the adipocyte differentiation process, as assessed by measuring fat accumulation using Oil-red O staining. In addition, dichloromethane fractions from I. verum Hooker fil. reduced important adipogenic transcription factors, such as CCAAT/enhancer binding protein α (C/EBPα), C/EBPβ, and peroxisome proliferator activated receptor γ (PPARγ). The expression of FAS and LPL, which are terminal differentiation markers of mature adipocytes, was also reduced in the 3T3-L1 adipocytes treated with dichloromethane fractions from I. verum Hooker fil. In addition, the treatment significantly inhibited mitotic clonal expansion, which is essential for adipocyte differentiation, by arresting the G1 phase of the cell cycle. Taken together, these results suggest that dichloromethane fractions from I. verum Hooker fil. may be a natural material with antiobesity effects.

대구 경북 지역 강직성척추염 환자의 방사선학적 중증도와 관련된 임상인자 분석

정현영 ( Hyun Young Jung ),이화정 ( Hwa Jeong Lee ),최정윤 ( Jung Yoon Choe ),박성훈 ( Sung Hoon Park ),김성규 ( Seong Kyu Kim ),김상현 ( Sang Hyon Kim ),김주연 ( Ju Youn Kim ),한승우 ( Seung Woo Han ),김성호 ( Seong Ho Kim ) 대한류마티스학회 2010 대한류마티스학회지 Vol.17 No.4

Objective: This study was designed to identify prognostic determinants of radiographical severity in patients with ankylosing spondylitis (AS) living in Daegu and Kyungpook territories. Methods: One hundred-nineteen patients with AS were consecutively enrolled from four regional general hospitals. Clinical data including smoking habits, alcohol intake, disease duration, HLA-B27 positivity, involvement of peripheral joints, occupational activity, and regular exercise were investigated. Radiographical severity was assessed by the Modified Stoke Ankylosing Spondylitis Spinal Score (mSASSS). Statistical analyses were performed using the independent Student`s t-test, Pearson`s correlation coefficient analysis, and multivariate regression analysis. Results: Radiographical severity, as evaluated with the mSASSS, was associated with age of disease onset (p=0.011) and disease duration (p<0.001). Additionally, mSASSS was significantly higher in males than females (p=0.039) and in patients without involvement of ankle arthritis than those with involvement of ankle arthritis (p=0.026). Patients with hip arthritis had significantly higher mSASSS scores than those without hip arthritis. A multivariate regression analysis showed that older age of disease onset, male gender, and disease duration were independent predictors of AS radiographical severity (p<0.001, p=0.013, and p<0.001, respectively). Hip arthritis was an independent predictor of C-spine radiographical severity in AS (p=0.031). Conclusion: Radiographical severity of the prognosis was determined by age of disease onset, gender, and disease duration in patients with AS living in Daegu and Kyungpook territories.

학교급식 조리종사자의 직무스트레스와 이직의도 간의 관계 분석

나은정 ( Eun Jeong Na ),김현아 ( Hyun Ah Kim ),정현영 ( Hyun Young Jung ) 한국식품조리과학회 2009 한국식품조리과학회지 Vol.25 No.5

맥동전류 검출이 가능한 누전차단기 알고리즘 설계

임종웅(Jong-Ung Lim),정현영(Hyun-Young Jeong),박진영(Jin-Young Park),안길영(Kil-Young Ahn),김영근(Young-Geun Kim) 대한전기학회 2021 대한전기학회 학술대회 논문집 Vol.2021 No.5

본 논문에서는 맥동전류 검출이 A형 누전차단 알고리즘에 대하여 제안하였다. A형 누전검출 알고리즘은 (+), (-)의 Peak비, 기본파, RMS, 3고조파를 이용하여 구현하였으며, 동작실험을 통하여 정현파 및 맥동전류(0°,90°,135°) 검출 알고리즘의 실효성을 입증하였다.

돌미나리씨 추출물에 의한 3T3-L1 지방전구세포의 분화 억제

지향화(Hyang Hwa Ji),정현영(Hyun Young Jeong),진수정(Soojung Jin),권현주(Hyun Ju Kwon),김병우(Byung Woo Kim) 한국생명과학회 2012 생명과학회지 Vol.22 No.12

돌미나리(Oenanthe javanica)는 뛰어난 해독작용과 항균작용으로 예로부터 약재로 사용되어 왔으나 돌미나리씨의 활성은 아직 밝혀지지 않았다. 본 연구에서는 3T3-L1 지방전구세포를 사용하여 돌미나리씨 메탄올 추출물(OJSE)의 지방세포분화에 미치는 영향과 그 기전에 대해 조사하였다. 세포독성이 없는 농도(1~200 μg/ml)의 OJSE를 지방세포 분화유도제와 동시에 지방전구세포에 처리하여 분화시킨 후 Oil Red O 염색을 한 결과 세포 내 lipid droplet 생성 및 triglyceride 축적이 OJSE 농도의존적으로 감소되었으며, 지방세포분화과정에 중요한 역할을 하는 지방세포특이적 마커인 CCAAT/enhancer binding proteins α, β (C/EBPα, C/EBPβ) 및 peroxisome proliferator-activated receptor γ (PPARγ)의 발현이 현저하게 저하되었다. 이는 OJSE에 의해 지방세포 분화 관련 전사인자의 발현이 효과적으로 억제되어 지방세포로의 분화가 저해되고 결과적으로 지방세포내 lipid droplet 생성 및 triglyceride 축적이 억제되었다는 것을 시사한다. 또한 OJSE처리에 의해 mitotic clonal expanstion 단계에서의 세포증식이 저해되었으며, 세포주기의 변화를 분석한 결과, OJSE처리에 의해 지방전구세포의 G1 arrest가 유발됨을 확인하였다. 세포주기 관련 단백질 발현을 분석한 결과, OJSE 농도의존적으로 Cdk inhibitor인 p21의 발현이 현저하게 증가되었으며, 반면 cyclin E, Cdk2, E2F-1 및 phospho-Rb의 발현은 저하됨을 알 수 있었다. 이러한 연구 결과들에 의해 OJSE는 지방전구세포의 G1 arrest를 유도하고 지방세포분화 관련 단백질의 발현을 억제하여 지방세포로의 분화를 억제하는 항비만 효능을 갖는 천연 소재임을 확인하였고, 본 연구는 이를 활용한 향후 지속적인 연구를 위한 기초자료로 그 가치가 매우 높을 것으로 생각된다. Oenanthe javanica has been used as a food source and also in traditional folk medicine for its detoxifying properties and anti-microbial effects since ancient times. In this study, we evaluated the effect and mechanism of O. javanica seed methanol extract (OJSE) on adipocyte differentiation by 3T3-L1 preadipocytes. Under non-toxic conditions, OJSE treatment resulted in a dose-dependent inhibition of lipid droplet generation and triglyceride accumulation by suppressing adipocyte differentiation, which are associated with the decreased expression of key proadipogenic transcription factors including CCAAR/enhancer binding protein α, β (C/EBPα, C/EBPβ) and peroxisome proliferator-activated receptor γ (PPARγ). OJSE also significantly inhibited proliferation and differentiation of 3T3-L1 preadipocytes through G1-phase arrest, indicating that OJSE blocked mitotic clonal expansion during adipocyte differentiation. Investigation of the alteration of G1 phase arrest-related proteins indicated a dose-dependent increase in the expression of p21 and reduction in expression of cyclin E, Cdk2, E2F-1 and phospho-Rb by OSJE. Taken together, these results suggest that OJSE inhibits adipocyte differentiation by blocking the mitotic clonal expansion, which is accompanied by preadipocyte cell cycle arrest.