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쥐 뇌의 Q-dianisidine Peroxidase의 특성 및 부분 정제
정태숙,이명은,최명언,Jeong, Tae-Sook,Lee, Myung-Eun,Choi, Myung-Un 생화학분자생물학회 1986 한국생화학회지 Vol.19 No.1
뇌에서의 catecholamine 대사와 $H_2O_2$ 이용에 관한 기초 연구로 뇌의 peroxidase를 용해시켜 분광학적 방법으로 그 특성을 연구하였다. 쥐 뇌의 peroxidase를 0.05% Triton X-100, 0.2 M KCl를 포함하는 pH 7.4의 완충용액으로 용해시켰으며, 이 용해된 효소는 pH 5.2-5.3 사이에서 최고 활성도를 보였고 기질인 O-danisidine과 $H_2O_2$에 대한 $K_m$ 값은 각각 0.19 mM과 7.0 mM로 얻어졌다. 이들 기초 결과로부터 용해된 효소의 표준분석조건을 수립하였다. 이 효소의 정제방법으로 sephadex G-200 젤 여과법과 DEAE-cellulose 이온교환 크로마토그래피법을 사용하였으며 30% 회수율로 27배 가량의 부분 정제가 이루어졌다. 또한 이 효소의 기질에 관한 특수성도 살펴 보았다. For a basic study of catecholamine metabolism and of the utilization of hydrogen peroxide in brain. rat brain peroxidase was solubilized and its properties were examined spectrophotometrically. The peroxidase was solubilized in 0.01 M phosphate buffer, pH 7.4 containing 0.05% Triton X-100 and 0.2 M KCl. The $K_m$ values of brain peroxidase for O-dianisidine and $H_2O_2$ were 0.19 mM and 7.0 mM respectively. From these basic results, the standard assay condition of solubilized enzyme was established. The peroxidase was partially purified from solubilized protein by sephadex G-200 gel filtration and DEAE-cellulose ion exchange chromatography. The specific activity of brain peroxidase was increased 27-fold with 30% recovery. Substrate specificity of the enzyme was also investigated.
쥐 뇌의 O - dianisidine Peroxidase 의 특성 및 부분 정제
정태숙,이명은,최명언 ( Tae Sook Jeong,Myung Eun Lee,Myung Un Choi ) 생화학분자생물학회 1986 BMB Reports Vol.19 No.1
For a basic study of catecholamine metabolism and of the utilization of hydrogen peroxide in brain, rat brain peroxidase was solubilized and its properties were examined spectrophotometrically. The peroxidase was solubilized in 0.01 M phosphate buffer, pH 7.4 containing 0.05% Triton X-100 and 0.2 M KCI. The K_m values of brain peroxidase for 0-dianisidine and H₂O were 0.19 mM and 7.0 mM respectively. From these basic results, the standard assay condition of solubilized enzyme was established. The peroxidase was partially purified from solubilized protein by sephadex G-200 gel filtration and DEAE-cellulose ion exchange chromatography. The specific activity of brain peroxidase was increased 27-fold with 30% recovery. Substrate specificity of the enzyme was also investigated.
정태숙(Jeong, Tae-Sook),박선희(Park, Sun-Hee),김영희(Kim, Yeong-Hee) 충북대학교 생활과학연구소 2017 생활과학연구논총 Vol.21 No.2
This study examined the relative contributions of socio-demographic factors, physical health, mental health, family communication, and family conflict to family happiness across age groups. The participants of the study were 560 adults having at least one child under the age of 18. Data were analyzed through hierarchical regression using SPSS 18.0. For participants in their thirties, physical health and family communication were positively associated with family happiness, whereas family conflict was negatively associated with family happiness. For participants in their forties, family happiness was associated with family communication, mental health, and family conflict, in that order. Family monthly income in particular showed a substantial positive association with family happiness for participants in their fifties. Further, family communication, family conflict, and mental health were, in that order, related to family happiness for participants in their fifties. Family communication was the most strongly associated with family happiness regardless of the age group. These findings extend previous research on happiness in cultural contexts.
순무(Brassica rapa ssp.) 뿌리로부터 flavonoid의 분리 및 동정
정락훈 ( Rak Hun Jeong ),( Qian Wu ),조진경 ( Jin Gyeong Cho ),이대영 ( Dae Young Lee ),( Sabina Shrestha ),이민호 ( Min Ho Lee ),이경태 ( Kyung Tae Lee ),최명숙 ( Myung Sook Choi ),정태숙 ( Tae Sook Jeong ),안은미 ( Eun Mi 한국응용생명화학회(구 한국농화학회) 2013 Journal of Applied Biological Chemistry (J. Appl. Vol.56 No.1
순무뿌리(Brassica rapassp)를 실온에서 95% ethanol 수용액으로 추출하고 이 추출물을 ethyl acetate (EtOAc)분획, n-butyl alcohol 분획 및 H2O 분획으로 나누었다. EtOAc분획에 대하여 silica gel, ODS 및 Sephadex LH-20 column chromatography 를 반복실시 하여 5종의 flavonoid를 분리하였다. NMR, IR 및 MS data를 해석하여 각각 lic℃halcone A (1), 4,4`-dihydroxy- 3`-methoxychalcone (2), liquiritigenin (3), liquiritin (4), isoliquiritin (5)으로 구조동정하였다. 이들 화합물들은 순무뿌리에서는 처음으로 분리되었다. The roots of Brassica rapa ssp. were extracted with 95% aqueous ethanol and the concentrated extracts were partitioned using ethyl acetate (EtOAc), n-butyl alcohol and H2O, successively. From the EtOAc fraction, five flavonoids were isolated through repeated silica gel and ℃tadecyl silica gel (ODS) column chromatography (c.c.). Based on NMR, mass spectrometry (MS) and IR spectroscopic data, the chemical structures of the compounds were determined to be lic℃halcone A (1), 4,4`- dihydroxy-3`-methoxychalcone (2), liquirtigenin (3), liquiritin (4), and isoliquiritin (5). This is the first report of these compounds isolated from the root of this plant.
식용식물자원으로부터 활성물질의 탐색 XVI. -사자발쑥(Artemisia herba)의 전초로부터 sterol 화합물의 분리-
방면호,정해곤,송명종,유종수,정선아,이대영,김세영,정태숙,이경태,최명숙,백남인,Bang, Myun-Ho,Chung, Hae-Gon,Song, Myoung-Chong,Yoo, Jong-Su,Chung, Sun-A,Lee, Dae-Young,Kim, Se-Young,Jeong, Tae-Sook,Lee, Kyung-Tae,Choi, Myung-Sook,Baek, 한국응용생명화학회 2006 Applied Biological Chemistry (Appl Biol Chem) Vol.49 No.2
사자발쑥의 전초를 80% MeOH 용액으로 추출하고, 얻어진 추출물을 EtOAc, n-BuOH 및 물로 용매 분획 하였다. 이 중 EtOAc 분획과 n-BuOH 분획으로부터 silica gel과 octadecyl silica gel(ODS) column chromatography로 정제하여 4종의 sterol 화합물을 분리하였다. 각 화합물의 화학구조는 NMR, MS 및 IR 등의 스펙트림 데이터를 해석하여, ${\beta}-sitosterol$ (1), dehydroergosterol $5{\alpha},8{\alpha}-epidioxide$ (2), stigmasterol (3), daucosterol (4)으로 동정하였다. 이 화합물들은 사자발쑥에서 처음 분리되었다. Sajabalssuk (Artemisia herba) was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and $H_2O$, successively. From the EtOAc and n-BuOH fractions, four sterols were isolated through the repeated silica gel and ODS column chromatographies. From the results of physico-chemical data including NMR, MS and IR, the chemical structures of the sterols were determined as ${\beta}-sitosterol$ (1), ergosterol peroxide (2), stigmasterol (3) and daucosterol (4). They were the first to be isolated from Sajabalssuk (Artemisia herba).
식용식물자원으로부터 활성물질의 탐색-XII. - 꽃마리(Trigonotis peduncularis Benth.)로부터 Flavonol 배당체의 분리 및 hACAT1 저해활성 -
양혜정,송명종,방면호,이진희,정인식,이윤형,정태숙,권병목,김성훈,김대근,박미현,백남인,Yang, Hye-Joung,Song, Myoung-Chong,Bang, Myun-Ho,Lee, Jin-Hee,Chung, In-Sik,Lee, Youn-Hyung,Jeong, Tae-Sook,Kwon, Byoung-Mog,Kim, Sung-Hoon,Kim, Dae-Keu 한국응용생명화학회 2005 Applied Biological Chemistry (Appl Biol Chem) Vol.48 No.1
꽃마리를 80% MeOH로 추출하고, 얻어진 추출물을 EtOAc, n-BuOH 및 $H_2O$로 용매 분획하였다. EtOAc와 n-BuOH 분획에 대하여 column chromatography를 반복하여 4종의 flavonol 배당체를 분리하였다. 각각에 대하여 2D-NMR을 포함한 스펙트럼 데이터의 해석과 문헌 자료를 조사하여 $kaempferol-3-O-{\beta}-{D}-glucopyranoside(astragalin),\;kaempferol-3-O-{\alpha}-{L}-rhamnopyranosyl(1{\rightarrow}6)-{\beta}-{D}-glucopyranoside(nicotiflorin),\;quercetin-3-O-{\alpha}-{L}-rhamnopyranosyl(1{\rightarrow}6)-{\beta}-{D}-glucopyranoside(rutin),\;quercetin-3-O-{\beta}-{D}-glucopyranoside(isoquercitrin)$로 구조를 결정하였다. 이 화합물들은 꽃마리에서는 이번에 처음 분리, 보고되었다. 또한 $nicotiflorin(100\;{\mu}g/ml)$은 hACAT1에 대하여 $68.3{\pm}1.2%$ 저해활성을 나타내었다. The MeOH extracts obtained from whole plant of Trigonotis peduncularis Benth. were solvent fractionated using EtOAc, n-BuOH and water, successively. The EtOAc and n-BuOH fractions gave four flavonol glycosides through application of silica gel and octadecyl silica gel (ODS) column chromatographies. The chemical structures of the flavonol glycosides were determined by the interpretation of several spectral data including 2D-NMR as $kaempferol-3-O-{\beta}-{D}-glucopyranoside\;(astragalin,\;1),\;kaempferol-3-O-{\alpha}-{L}-rhamnopyranosyl\;(1{\rightarrow}6)-{\beta}-{D}-glucopyranoside\;(nicotiflorin,\;2),\;quercetin-3-O-{\alpha}-{L}-rhamnopyranosyl(1{\rightarrow}6)-{\beta}-{D}-glucopyranoside\;(rutin,\;3),\;quercetin-3-O-{\beta}-{D}-glucopyranoside\;(isoquercitrin,\;4)$. The flavonoids have been first isolated from this plant. Nicotiflorin $(100\;{\mu}g/ml)$ showed $68.3{\pm}1.2%$ of the inhibitory effect on hACAT1(human Acyl CoA: cholesterol transferase 1) activity.