면역기능 증강 신물질에 대한 마우스의 면역학적 및 혈액학적 변화

정지윤,Jung, Ji-Youn 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.4

Immu-Forte composed of chitosan, ${\beta}-glucan$, manno-oligosaccharide and pangamic acid was evaluated for its effectiveness as a nonspecific immunostimulator in mice. The effects of Immu-Forte were determined by analysis of cytokines using ELISA and phenotype of leukocyte subpopulations using monoclonal antibodies specific to mouse leukocyte differentiation antigens and flow cytometry. All T cells, all B cells, CD4 T cells, CD8 T cells, macrophages, IL-2, IL-4, IL-12 and IFN-r in Immu-Forte A-treated group increased in 1 months posttreatment and were significantly higher (p < 0.05) than that of control at 1 months posttreatment. All T cells, all B cells, CD4 T cells, CD8 T cells, macrophages and IL-2 in Immu-Forte EX-treated low and middle dose groups increased in 1 months posttreatment and were significantly higher (p < 0.05) than that of control at 1 months posttreatment. In the Immu-Forte soybean-treated group, NK cells and IL-4 were significantly higher in middle dose-treated group, and IL-2, IL-4 and IFN-r were significantly higher in low dose-treated group. In the Immu-Forte F-treated group, all T cells, all B cells, CD4 T cells, CD8 T cells, macrophages, NK cells, IL-2, IL-4, IL-12 and IFN-r in high dose-treated group and all T cells, all B cells, CD4 T cells, CD8 T cells, macrophages, IL-2, IL-4, IL-12 and IFN-r in middle dose-treated group and NK cells, IL-2, IL-4, IL-12 and IFN-r in low dose-treated group were significantly higher (p < 0.05) than that of control at 1 months posttreatment. In conclusion, this study has demonstrated that Immu-Forte had an immunostimulatory effect on mice through proliferation and activation of mouse immune cells.

Intralipidos에 대한 변이원성시험

정지윤,이원우,임종희,남정석,제정환,이광훈,강병철,이병희,박재학,이영순,Jung, Ji-Youn,Lee, Won-Woo,Ihm, Jong-Hee,Nam, Jeong-Seok,Che, Jeong-Hwan,Li, Guang-Xun,Kang, Byeong-Cheol,Yi, Beoung-Hi,Park, Jae-Hak,Lee, Yong-Soon 한국독성학회 1998 Toxicological Research Vol.14 No.3

In order to evaluate the mutagenic potential of Intralipidos produced by Greenmate cooperation. We performed Salmonella typhimurium reversion assay, chromosomal aberration test on chinese hamster ovarian cells and in vivo micronucleus assay using mouse bone marrow cells. In the reverse mutation test using Salmonella typhimurium TA98 and TA100, Intralipidos did not increase the number of revertant at any of the concentration tested in this study. Intralipidos did not increase the number of cells having structural or numberical chromosome aberration in cytogenetic test. In mouse micronucleus test, no significant increase were observed in the occurrence of micornucleated polychromatic erythrocytes in ICR male mice intraperitoneally administered with Intralipidos. These results indicate that Intralipidos has no genetic toxicity under these experimental conditions.

실험동물에서의 환경호르몬 물질의 생체내 영향 및 검색법 정립에 대한 연구

정지윤,이영순,Jung, Ji-Youn,Lee, Yong-Soon 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.4

The major protocol features of the rodent uterotrophic assay have been evaluated using a range of reference chemicals. The protocol variables considered include the selection of the test species and route of chemical administration, the age of the test animals, the maintenance diet used, and the specificity of the assay for estrogens. The rodents were ovariectomized under general anesthesia via bilateral flank incisions and randomly assigned to groups of 5 animals. Chemicals were DEHP, DBP, BPA and NP, were injected sc once daily with combinations of chemicals treatments for 3 days. In the results, the reported estrogenic chemicals DEHP and DBP were both negative in the single dose treatments. But, in the combinations of chemicals treatments, DEHP and DBP increased in bud number of mammary gland. Treatment of ovariectomized mice with combinations of other chemicals resulted in uterine and vaginal hyperplasia. The additive estrogenic effects were seen with the combinations of $17{\beta}$-Bestradiol and DBP treatment. the competitive estrogenic effects were seen with the combinations of $17{\beta}$-Bestradiol and nonylphenol, $17{\beta}$-Bestradiol and bisphenol-A treatments. These results offers a sysmatic and mechanistically informative approach to assessing estrogenicity. it provides a useful profile of activity using a reasonable amount of resources and is compatible with the study of individual chemicals as well as the investigation of interactions among combinations of chemicals. The results described illustrate the intrinsic complexity of evaluating chemicals for estrogenic activities and conform the need for rigorous attention to experimental design and criteria for assessing estrogenic activity.

Comparative Study on Picryl chloride (PCL)-induced Contact Dermatitis in Male and Female IQI/Jic Mice

Ji-Youn Jung(정지윤) 한국실험동물학회 2007 한국실험동물학회 학술발표대회 논문집 Vol.2007 No.-

Comparative Study on Picryl Chloride (PCL)-induced Contact Dermatitis in Female IQI/Jic and BALB/c Mice

Ji-Youn Jung(정지윤) 한국실험동물학회 2013 한국실험동물학회 학술발표대회 논문집 Vol.2013 No.8

기획특집 : 구제역

정지윤 ( Ji Youn Jung ) 한국식품위생안전성학회 2011 Safe Food Vol.6 No.1

혈청 CA 125가 상승된 Meigs씨 증후군의

지은정 ( Eun Jung Ji ),한정미 ( Jung Mi Han ),문철용 ( Cheol Yong Moon ),정지윤 ( Ji Youn Jung ),남은숙 ( Eun Sook Nam ),장봉림 ( Pong Rheem Jang ),이근영 ( Keun Young Lee ),권용일 ( Yong Il Kwon ) 대한산부인과학회 2008 Obstetrics & Gynecology Science Vol.51 No.8

Meigs` syndrome is a benign ovarian tumor associated with ascites and pleural effusion. Elevated CA 125 in Meigs` syndrome is unusual clinical condition reported in few cases. We report here, a 49-year-old postmenopausal woman with right ovarian fibrothecoma with ascites, right pleural effusion and high serum levels of CA 125. Although postmenopausal women with ovarian tumor, ascites, pleural effusion, and elevation of CA 125 levels probably have malignant ovarian tumors, Meigs` syndrome must be considered in the differential diagnosis.

〈소쇄원30영(瀟灑園三十詠)〉을 통하여 본 소쇄원 外園의 공간구성

천득염(Cheon, Deuk-Youm),정지윤(Jung, Ji-Youn) 대한건축학회 2018 대한건축학회논문집 Vol.34 No.3

The purposes of this study are to analyze the spatial composition of the outer garden of Soswaewon that appears in Gyeong-Ji Yang’s “30 Poems of Soswaewon” and examine its significance and symbols. These poems by Gyeong-Ji Yang are important historical records that portray the appearance of Soswaewon and the space outside it at the end of the 17th century. Unlike “48 Poems of Soswaewon” or “Soswaewondo,” these poems arenot limited to Soswaewon and its surroundings; theyalso include areas from the village entrance upwardto the peak of the mountain behind the village. These poems can be divided into three main parts. Jiseok-ri and Changam-dong in poems 1 and 2 provide first impressions of the village in which the main Soswaewon garden is located, whereas poems 3 to 8 and poems 10 to 12 describe the space inside Soswaewon. Poems 13 to 30 depict the beautiful scenery around the outer garden of Soswaewon. In general, about 30% of the compilation in “30 Poems of Soswaewon” celebrates the inner Soswaewon, and the remaining 70% depicts the scenery around Soswaewon (i.e., views of the outer garden). It is presumed that Gyeong-Ji Yang employed this structure to achieve some type of order. Between the “48 Poems of Soswaewon”-also describing Soswaewon-and “Soswaewondo,” which is a woodprint drawing of Soswaewon, “30 Poems of Soswaewon” offers the widest coverage of Soswaewon. Compared to “48 Poems of Soswaewon,” which addresses the scene inside Soswaewon, new buildings such as the Jaeweoldang, Buhweondang, and Hancheonsa appear in “30 Poems of Soswaewon.” Thus, the latter covers a much wider range. Chimgye Munbang - Gwangpunggak, Yangdandongo - Aeyangdang, Byeongseogjuggeun - Jajukchong, etc. are mentioned in both compilations. The use of a wide range of natural objects in these 30 poems, including mountains, birds, and the sky (instead of trees and flowers), shows that the author perceived Soswaewon as an area with a much higher and wider view than that of a typical living space. Moreover, natural regression ideologies, or seclusion ideologies, are evident.

Resveratrol이 MDA-MB-231 유방암 세포에서 PI3K/Akt와 p53 신호경로를 통한 apoptosis 유도

권중기(Jung-Ki Kwon),박영석(Young-Seok Park,),박병권(Byung-Kwon Park),김병수(Byeong-Soo Kim),김상기(Sang-Ki Kim),정지윤(Ji-Youn Jung) 한국식품과학회 2012 한국식품과학회지 Vol.44 No.4

본 연구는 MDA-MB-231 세포에서 resveratrol의 apoptosis 유발효과에 대해 알아보기 위해 연구되었다. Cell viability 결과 농도 유의적으로 감소하였으며, DAPI stain에서는 농도 의존적으로 chromatin condensation이 증가하는 것을 확인하였다. Resveratrol은 p53, cleaved-caspase-3, cleaved-caspase-9의 발현을 증가시켰지만, PI3K/Akt의 발현은 시간 의존적으로 감소시켰다. In vivo 실험에서는 resveratrol의 종양 억제 효과를 확인하였다. 50 mg/kg 투여한 군에서 종양의 크기가 대조군에 비해 감소하였으며, TUNEL assay를 통해 apoptosis cell을 관찰한 결과 50 mg/kg 투여한 군에서 많이 관찰되었다. 면역조직화학 염색을 통해 50 mg/kg 투여한군에서 p53, cytochrome-C, cleaved-caspase-3의 발현이 증가하는 것을 확인하였다. 본 연구의 결과를 종합하여 봤을 때 resveratrol이 MDA-MB-231 세포에 apoptosis를 유발하는데 효과가 있는 것으로 사료된다. This study was conducted in order to investigate the effect of resveratrol on the induction of apoptosis in MDAMB-231 breast cancer cells. The result of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl terazolium bromide (MTT) assay shows that cell viability significantly decreased in a dose and time-dependent manner. 4,6-diamidino-2-phenylindole (DAPI) staining shows significantly increased chromatin condensation in a dose and time-dependent manner. Resveratrol increased the expression of p53, cleaved-caspase-3, and cleaved-caspase-9, whereas the expression of PI3K/Akt decreased in a timedependent manner. We investigated the in vivo tumor growth inhibitory effect of resveratrol. Tumor volume was significantly decreased in the 50 mg/kg resveratrol-administration group compared to the control group. In the 50 mg/kg treated group. Apoptosis cells were frequently observed by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) assay. Immunohistochemistry staining shows increased the expression of p53, cytochrome-C, and cleaved-caspase-3 in the 50 mg/kg treated group. These results indicate that resveratrol induced apoptosis through PI3K/Akt and p53 signal pathway in MDA-MB-231 cell.

한국석탑의 세부 명칭과 의미 고찰

천득염,정지윤,오창명,Cheon, Deuk-Youm,Jung, Ji-Youn,Oh, Chang-Myeong 한국건축역사학회 2018 건축역사연구 Vol.27 No.3

The detailed names and meanings of Korean stone pagodas have not yet been properly studied or clearly defined. Therefore, an aim of this report is to compare the detailed names of Korean, Chinese, and Japanese stone pagodas by focusing on content used previously by Ko and his students and content that is currently in use (i.e., since the Japanese colonial era). Additional objectives are to assess the significance of these names and propose an appropriate detailed draft regarding names of stone pagodas. The discussion will proceed by dividing the stone pagoda into three parts: the podium, main structure, and finial. First, the content in Luo Zhewen(羅哲文)'s "Ancient pagodas in China(中國古塔)" (1985) is considered; then, "朝鮮の石塔" (1943)-published by during the Japanese colonial era-is explored. The thesis written by Yu-Seop Ko regarding Korea's first stone pagoda and the content in "A Study of Korean Pagodas(朝鮮塔婆의 硏究)" (1948), which contains Yu-Seop Ko's posthumous works published in book form by his student Su-Yeong Hwang, Heu-Kyoung Kim are examined. to pagodas that are used today, are compared and reviewed. Since Korea, China, and Japan influence each other, pagoda-related terms are shared to some extent. In this study, these were applied to stone pagodas, and names used thus far that have not drawn criticism were studied and proposed as suitable alternatives.