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RISS 인기검색어
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- 저자 : 정재화(Jae Hwa Cheong) (검색결과 2 건)
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HL-60 세포주, 제대혈 및 골수 단핵구 세포의 interferon-gamma에 대한 감수성에 관한 연구
정희정,홍대식,김숙자,정재화,이주영,이남수,박성규,원종호,박희숙,김성일,Cheong, Hee Jeong,Hong, Dae Sik,Kim, Sook Ja,Cheong, Jae Hwa,Lee, Joo Young,Lee, Nam Su,Park, Sung Kyu,Won, Jong Ho,Park, Hee Sook,Kim, Sung Il 대한면역학회 2001 Immune Network Vol.1 No.3
Background: Finding of the regulation of various gene expression by cytokine including $IFN-{\gamma}$ in hematopoietic stem cell will light up the understanding of pathogenesis of aplastic anemia in various aspects. To study on aplastic anemia, however, we have to circumvent the difficulty of directly obtaining bone marrow stem cells from the patient. Therefore, we tried to find out a cell can replace the bone marrow stem cells for study on cell signaling pathway and regulation of gene expression by $IFN-{\gamma}$. Materials and Methods: HL-60 cells, of 20 ng/mL of $IFN-{\gamma}$. Total RNA was isolated from the cells and RT-PCR of the indoleamine 2,3-dioxygenase (IDO), $IFN-{\gamma}$, TNF-${\alpha}$, $MIP-1{\alpha}$, and $TGF-{\beta}2$ was carried out for the estimation of the gene expression. Results: $IFN-{\gamma}$ induced IDO gene expression of mononuclear cells from umbilical cord blood showed similar pattern as compared to that of bone marrow. Whether $INF-{\gamma}$ was treated or not, $TNF-{\alpha}$ was expressed in both mononuclear cells from umbilical cord blood and bone marrow. However, HL-60 cells showed different expression patterns. HL-60 cells would express neither IDO nor $TNF-{\alpha}$ even under the culture with 20ng/mL of $IFN-{\gamma}$. Conclusion: Our results showed bone marrow can be replaced with mononuclear cells from umbilical cord blood in the study on the relation between aplastic anemia and $IFN-{\gamma}$ including $IFN-{\gamma}$ cell signaling pathway.
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동아시아 FTA Hub 전략의 경제적 효과와 타당성 검토
정재화,정인교 韓國東北亞經濟學會 2009 동북아경제연구 Vol.21 No.1
East Asian countries have continued to pursue an active FTA policy since the 1997 Asian financial crisis. In the region, ASEAN+1 type of FTAs are already in effect or have been concluded between the ASEAN and the three Northeast Asian countries (Korea, Japan, China) along with the deeper integration of the AFTA which combines the ten ASEAN member countries. At the same time, active discussions have been conducted on a possible FTA that covers the entire East Asian region, an East Asia FTA (EAFTA). If this EAFTA is concluded, it is expected to surpass the size of NAFTA and to have even greater ripple effects. According to the CGE analysis on the economic effects of an East Asia FTA (EAFTA), the FTA is expected to be beneficial to the countries in the region. A regional FTA among countries in Northeast Asia or East Asia will have greater rewards than bilateral FTAs between two countries. In particular, Korea is deemed to be the greatest beneficiary of an EAFTA. And if China and Japan do not conclude an FTA and Korea concludes FTAs with ASEAN China and Japan and becomes an East Asian FTA hub, the economic effects are expected to be even greater. Notwithstanding the economic benefits that come from being an FTA hub, the merits of an East Asia FTA covering the entire region will be even greater. First of all, it will be possible to lower the rising transaction costs that ensue from the intertwined regional FTA network Also, higher economic efficiency is expected in the mid-term and long-term from the harmonization of the different systems. Therefore, the straightforward strategy for Korea would be to pursue an East Asia FTA and strategically promote FTAs with Japan and China to get a strategic advantage in the future East Asia FTA negotiation process. In fact, under the current situation in which an FTA among the three major countries in Northeast Asia, Korea, China and Japan, is deterred by various obstacles, an East Asia FTA could be the favored alternative. This is because an FTA combining the entire region could alleviate the conflicting interests of individual industry sectors that may arise from a bilateral FTA. In addition, it would be possible to establish a more flexible level of trade liberalization in goods under an East Asia FTA.
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