결막에서 분리한 Diphtheroid의 Plasmid Profile과 약제내성상과의 관계

정재규(Jae-Kyu Chung),김성광(Sung-Kwang Kim),박미경 (Mi-Kyung Park) 大韓微生物學會 1985 大韓微生物學會誌 Vol.20 No.2

원저 : 항산균의 항결핵제내성에 관한 연구 (제1보) 토양항산균의 항결핵제에 대한 저항성

정재규 ( Jae Kyu Chung ) 대한결핵 및 호흡기학회 1958 Tuberculosis and Respiratory Diseases Vol.6 No.-

Gram음성 장내세균에 대한 β-Lactamase 억제제의 항균증진 효과

신규창(Kyu-Chang Shin),김성광(Sung-Kwang Kim),김희선(Hee-Sun Kim),정재규(Jae-Kyu Chung) 大韓微生物學會 1990 大韓微生物學會誌 Vol.25 No.4

綠膿菌의 Oxidase 反應에 對하여

鄭在奎,朴商煥,金正壽 慶北大學校 醫科大學 1961 慶北醫大誌 Vol.3 No.2

Pseudomonas species produce an abundant quantity of cytochrome and cytochrome oxidase and is utilized for the identification of this species from clinical specimen, Gaby, especially emphasized the use of p-amino-dimethyl aniline oxalate for the oxidase test of Ps. aeruginosa. In connection with aerobic respiration, catalase and perioxidase are also usually found in aerobic bacteria. In the present study, author examined toe enzyme catalase, perioxidase and cytochrome oxidase of pseudomonas sp. isolated from surgical wounds to investigate the relationships and the value of these tests. 1) Pseudomonas. sp. show somewhat marked catalase and perioxidase activity as comp are to the other enteric bacilli, however the detection of these enzyme have no diagnostic value. 2) Pseudomonas sp. form a strong indophenol oxidase and this was specific as compare to enteric forms, with a diagnostic value, Gaby reagent, Kovac's reagent, and Nadi reagent are sensitive in same degree. 3) The enzyme as well as pyocyanin formation are weakened by the transfer in the medium containing glucose or cystine.

Staphylococcus epidermidis의 성상과 Plasmid profile의 상관에 대해서

정재규,김성광,박미경,김희선 대한화학요법학회 1986 대한화학요법학회지 Vol.4 No.1

안결막 및 그 외 병소로부터 분리한 23주의 coagulase 음성 포도구균을 분류하고 항생제에 대한 감수성검사와 각균주의 plasmid분포의 검사를 통하여 EtBr처리에 의한 약제내성의 cure와 plasmid소실과의 상관성을 관찰하였다. penicillin G와 cephalothin에 대해서는 10%내외, ampicillin, noboviocin, erythromycin, tobramycin, gentamycin, kanamycin 및 amikacin에 대해서는 20~30%, 그리고, tetracycline 및 chloramphenicol에 대해서는 각 47.8%와 56.3%의 균주가 내성을 나타내었다. 공시균주 중 17주(73.9%)가 plasmid를 보유하고 있었고 tetrcycline내성주(다제내성주 포함) 11주는 2.9 Mdal의 plasmid를 가진 6주와 이보다 작은 plasmid를 가진 4주로 나누어졌으며, 후자의 4주 중에서는 tetracycline cured strains만 얻을 수 있었고 이들 Tccured strain들 중에는 plasmid소실이 수반되는 균주도 있었다. 2.9Mdal의 plasmid를 가지는 S_16주에서 얻은 cured strain들에서는 tetracycline cured strain들만 plasmid band가 소실되었다. Coagulase negative Staphylococci isolated from conjunctiva and other sources were classified and observed the relationship between antibiotics resistance patterns and plasmids profiles of these isolates, also antibiotics curing experiments were carried out with ethidium bromide in accompanying with the loss of plasmids. Most isolates belonged to Staphylococcus epidermidis(69.6%) and cephalothin was the most sensitive antibiotics(8.7%) next to penicillin, and chloramphenicol was the most resistant drug(56.5%) next to tetracycline. Seventeen strains harbored plasmids, and eleven tetracycline resistant strains divided into two groups by molecular size of plasmids, i.e. 6 strains of 2.9Mdal and 4 strains of less than this size. From 4 strains of latter group, only tetracycline cured strains were isolated with or without loss of plasmid, however, from one strain of former group, tetracycline, chloramphenicol and erythromycin cured strains in single or in combination with were isolated but only tetracycline cured strains were accompanied with the loss of plasmids compared to parent strain.

結核菌의 抗結核劑에 對한 抵抗性 : 第一報 分離菌의 抗結核劑에 對한 耐性

鄭在奎 慶北大學校 醫科大學 1958 慶北醫大誌 Vol.1 No.1

Sensitivity test was performed on various sources of samples from tuberculous patients, by the modification of Ogawa's indirect, direct and prompt methods. For the purpose of detecting the colonial difference of resistancy, 15 cases were examined by picking up 4 colonies from each culture, and subcultured on a series of drug containing media. A survey of 225 tests was carried out in 112 patients and the following results were obtained. ⑴ 53 cases were resistant against streptomycin (47.3%). Three ceses out of them were those who had never received any streptomycin therapy. ⑵ 12 cases were resistant against I.N.A.H. (14.4%) and 8 cases were resistant against P.A.S. (8.4%) among 83 cases. ⑶ No marked colonial difference of resistancy was observed. However, slight difference was detected in 5 cases out of 15.

마늘의 Candida sp. 에 對한 抗菌作用에 關해서

鄭在奎,金渭祥,朴佑淳,鄭弼壽 慶北大學校 1962 論文集 Vol.5 No.-

In the previous communication, the growth inhibitory action of garlic on C. albicans was reported and discussed about the candida distribution of Korean people. In the present study, the inhibitory and lethal effects of garlic on candida sp. were studied further detail and the resistance of oral candida sp. isolated from various age groups, to garlic was examined. (1) The growth inhibitory and lethal concentration of garlic juice on standard candida sp. were differ according to the species, however 0.1-0.2% concentraion of garlic juice in Sabouraud media inhibit the growth and 0.6-1.2% concertration of garlic juice exhibit a lethal effect on cardida sp. within 12 hours. (2) The resistance formation or a difference of resistance level of the isolated strain from various age group were not found and candida sp. seems to be not form resistance easily against garlic.

Candida ablicans에 對한 miconazole nitrate, nystatin 및 rifampin의 單獨 및 倂合抗菌作用에 關하여

鄭在奎 대한화학요법학회 1983 대한화학요법학회지 Vol.1 No.1

With 110 clinical isolates of Candida albicans, growth inhibitory actions of miconazole nitrate and nystatin alone or in combination with rifampin were evaluated, On Sabouraud glucose plate, miconazole nitrace combined with ridampin showed weak additive reaction against tyo straifls, indifferent t o four, and antagonistic to four, and when miconazob nitrate combined with nystatin, two strains showed weak additive, one was indifferent and seven showed antagonistic reactions, but all strains exhibited weak additive reaction when nystatin combined with rifampin. In Sabouraud broth, also weak reactions of both additive or antagonistic were observed according to the strains and to the combination of drugs. However. none of the drug combination exhibited clear results to be termed as synergistic or antagonisticc reaction.

紫外線으로 誘發시킨 C. Albicans의 變異性

鄭在奎 慶北大學校 1958 論文集 Vol.3 No.-

The suspension of C. abicans was irradiated under the sublethal dose of ultra-violet light and subcultured on a teterazolium chloride Sabouraud media to detect the tetrazolium reduction. In the course of irradiation, a few white colonies were noted which were markedly differing in growth rate and pigment reduction as compared with the normal colony. These were refered as W-colony arbitrarily in this report to distinguish these from the normal red colony (R Colony). Various characteristics of teh W and R colony are as follows: (1) No morphological differences such as pseudomycelium formation and Chlomydospore formation between them were noted. However, the growth rate of the was form was significantly slower than the R form. (2) There was no difference in sugar fermentation between R and W forms except for the W form are somehow slower as compared with the R form. (3) There was no difference in tetrezolium reduction between R and W forms substantially, when the examination was performed with a cell suspension, but on the tetrazolium solid Sabouroud media, the W forms formed white small colonies as compared with the R colonies. (4) The W-form showed markedly reduced toxicity in mice.

土壤抗酸菌의 生體內 藥濟耐性 獲得에 對하여

鄭在奎,李相準,文榮石 慶北大學校 1964 論文集 Vol.8 No.-

In order to observe the appearance of drug resistant strains in vivo, soil acid fast bacilli (s-12) which exhibited and appearance in high frequency of drug resistant strains in vitro experiments, were inoculated into the mice, and three days after inoculation these mice were followed by treatment with streptomycin and isonicotinic amide hydrazid for 2 to 3 weeks according to the groups. After the cessation of treatment, mice of the each group were sacrificed and the drugs treated, on both two and three weeks treated groups, with a single exception of high druges treated, on both and three weeks treated groups, with a single exception of high resistant variant from on the liver lesion of the 3 weeks treated isonicotnic amid hydrazid group.