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RISS 인기검색어
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- 저자 : 정일엽 ( Il Yup Chung ) (검색결과 5 건)
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Production of Interferon-$\gamma$ from Human Peripheral Blood Lymphocytes
정일엽,염영일,이상기,정태화,한문희,Chung, Il-Yup,Yeom, Young-Il,Rhee, Sang-Ki,Chung, Tai-Wha,Han, Moon-Hi 생화학분자생물학회 1986 한국생화학회지 Vol.19 No.3
인체혈액에서 분리한 임파구세포를 PHA-M, Con A, PWM등의 mitogen으로 자극시켜 여러가지 조건하에서 감마-인터페론을 생산하였다. 유도물질로 사용된 T-세포 mitogen인 PHA-M이나 Con A가 B-세포 mitogen 인 PWM보다 감마-인터페론 유도에 훨씬 효율적이었으며 이로부터 임파구세포 중에서 T-세포가 감마-인터페론을 생산하는 주요세포원 임을 확인하였다. 감마-인터페론 생산시 Con A의 적정농도는 $5-10\;{\mu}g/ml$이었다. 유도개시 약 48시간 경과 후 인터페론이 최대로 생산되었고 그 이후에도 인터페론의 활성은 감소되지 않았다. Phorbolester인 TPA를 첨가할 경우 Con A 또는 PHA-M 단독으로 인터페론을 유도시킨 것보다 10배 이상의 감마-인터페론의 생산이 증가되었다. 생산배양액에 첨가되는 혈청의 농도가 인터페론의 생산에 마치는 영향은 매우 극적이어서 태아 송아지 혈청농도 2%에서 최대로 생산되었고 농도가 증가함에 따라 생산역가는 급격히 떨어졌다. 혈청 대신 사용한 알부민 단백질도 비슷한 효과를 나타냈다. 혈액중에 임파구세포에 처리된 Con A가 DNA 합성을 촉진시키는 성질을 이용하여 $^3H$-thymidine 표식으로 cpm을 측정해 본 결과, 이와같은 현상은 10-20%의 높은 혈청농도에서 Con A가 과량의 혈청단백질과 흡착하여 실제적인 Con A 농도를 적정농도 이하로 떨어뜨리기 때문인 것으로 나타났다. Interferon-$\gamma$(IFN-$\gamma$) was produced from human peripheral blood lymphocytes (Hu-PBL) by stimulating them with various kinds of mitogens. T-cell mitogens such as Con A and PHA-M were much more potent than B-cell mitogen such as PWM in producing IFN-$\gamma$ from Hu-PBL. The combined treatment of TP A with these mitogens enhanced IFN-$\gamma$ production ten fold compared with the single treatment of each mitogen. Both DNA synthesis and IFN-$\gamma$ production yielded bell-shaped dose-response curves for a given population of Hu-PBL in response to Con A. The effect of fetal calf serum(FCS) on IFN-$\gamma$ production was found to be critical: titer of IFN-$\gamma$ produced was very low in the absence of FCS and reached peak point at 2% FCS, beginning to decrease precipitously with an increasing FCS concentration up to 20%. Using the relationship between IFN-$\gamma$ production and mitogenic stimulation, it was found that one of factors responsible for the decrease of IFN-$\gamma$ production at the high serum concentration could be the binding of Con A to serum proteins which rendered Con A to be removed from the production system.
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Proinflammatory Cytokines과 TGF-beta가 섬유모세포의 증식에 미치는 영향
김철 ( Chul Kim ),박춘식 ( Choon Sik Park ),김미호 ( Mi Ho Kim ),장헌수 ( Hun Soo Chang ),정일엽 ( Il Yup Chung ),기신영 ( Shin Young Ki ),어수택 ( Soo Taek Uh ),문승혁 ( Seung Hyuk Moon ),김용훈 ( Yong Hoon Kim ),이희발 ( Hi Bal 대한결핵 및 호흡기학회 1998 Tuberculosis and Respiratory Diseases Vol.45 No.4
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DBA/1JCrj Mouse에 있어서 콜라젠유도관절염에 관한 면역학적 고찰
박승규,이지연,정일엽,최용경,최인성,김효준 大韓免疫學會 1996 大韓免疫學會誌 Vol.18 No.3
There is growing evidence that a variety of cytokines are secreted by cells at the inflammation sites of rheumatoid arthritis (RA) and that CDS+ B cell, a minor subtype of B cell population producing natural autoantibodies, is implicated in pathogenesis of RA. In this study, we evaluated the both cytokne levels and change of CDS+ B cell population in the peripheral blood from DBA/lJCrj mice(H-2`) which are collagen-induced arthritis(CIA) susceptible strain. Surprisingly, the healthy DBA/1JCrj and MRL/Ipr/Ipr(H-2'`) mice which were autoimmune susceptible strains tested in this experiment, showed lower IL-4, IL-6 and IL-10 levels in serum by 60% than heal-thy normal mouse strains such as Balb/c(H-2d), C57BL/6(H-2') and outbred ICR mice. MRL/lpr/ 1pr mice in which onset of spontaneous autoimmune disease is dependent upon age were similar to healthy DBA/1JCrj mice in levels of the cytokines in the serum when they are young. After the CIA(for DBA/1JCrj) or the spontaneous autoimmune disease(for MRL/lpr/Ipr) had been developed in the susceptible mouse strains, the levels of IL-6 and IL-4 in the serum were increased to 1.8- and 13-fold, respectively, as compaired with those from control groups while level of IL-10 remained relatively constant. The elevated levels of IL-4- and IL-6, however, in the serum from mice with disease status were still below those of the healthy normal mouse strains. On the other hand, CDS+ B cell population in the peripheral blood, which were reported to be increased with the development of RA for human, was rather significantly decreased for the CIA-induced DBA/lJCrj mice as evidenced by FACS analysis. It could be due to the differences in the pathogenic mechanism between CIA and RA. Taken together, our results. suggest that the levels of both these cytokines and CDS+ B cells may be utilized as important diagnostic markers for arthritides.
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이상기,정태화,한문희,염영일,정일엽 생화학분자생물학회 1991 BMB Reports Vol.19 No.3
Interferon-γ (IFN-γ) was produced from human peripheral blood lymphocytes (Hu-PBL) by stimulating them with various kinds of mitogens. T-cell mitogens such as Con A and PHA-M were much more potent than B-cell mitogen such as PWM in producing IFN-γ from Hu-PBL. The combined treatment of TPA with these mitogens enhanced IFN-γ production ten fold compared with the single treatment of each mitogen. Both DNA synthesis and IFN-γ production yielded bell-shaped dose-response curves for a given population of Hu-PBL in response to Con A. The effect of fetal calf serum(FCS) on IFN-γ production was found to be critical: titer of IFN-γ produced was very low in the absence of FCS and reached peak point at 2% FCS, beginning to decrease precipitously with an increasing FCS concentration up to 20%. Using the relationship between IFN-γ production and mitogenic stimulation, it was found that one of factors responsible for the decrease of IFN-γ production at the high serum concentration could be the binding of Con A to serum proteins which rendered Con A to be removed from the production system.
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Centipede Scolopendra subspinipes multilans L. Koch로부터 항균력이 있는 Centipedin의 추출, 정제 및 특성연구
김기태,최진성,민천홍,정일엽,박경배,조기승 한양대학교 이학기술연구소 1999 이학기술연구지 Vol.1 No.-
항균력이 있는 화합물인 Centipeding을 지네 Scolopendra subspinipes multilans L. Koch로부터 지용성 유기용매로 추출하였고, silicic acid column과 high S cation exchange chromatography 및 reverse-phase HPLC 등을 사용하여 고순도로 정제하였다. Gram-positive bacteria인 Staphylococcus aureus ATCC 6538P 및 gram-negative인 Klebsiella pneumoniae ATCC 8308 균주에 대하여 강한 항균력을 나타내었다. Dragendorff 시약에 의한 발색반응을 시행한 결과 Centipedin의 aromatic ring에는 nitrogen이 없음을 확이하였고 IR, Mass, NMR 분석 등에 의하여 Centipedin의 분자량은 162.1, 화학적 구조는 hydroxyl기와 carbonyl기를 함유하는 -hydroxyisocumarin으로 확인되었다. UV-spectrum 검색결과에 의하면 최대 흡수파장이 243 ㎚임을 확인하였고, melting point는 122℃이었으며, 실온에서 3개월간 방치된 Centipedin이 여전히 항균활성 및 화학적 구조를 유지하여 물리화학적 및 생물학적으로 안정한 화합물임으 알 수 있었다. The compound which has antibiotic activity was extracted and purified from centipede Scolopendra subspinipes multilans L. Koch by diethyl ether, silicic acid column and high S cation exchange chromatography followed by reverse-phase HPLC. This compoung named "Centipedin" showed antibiotic activity aganist microorganisms such as Gram^(+) -bacteria Staphylococcus aureus ATCC 6538P and Gram^(-) -bacteria Klebsiella pneumoniae ATCC 8308. Dragendorff test showed that there is no aromatic nitrogen within the Centipedim. The molecular weight of the Centipedin was 162.1 daltons as determined by mass spectrometry. The instrumental analysis of IR and NMR techniques revealed the molecular structure of Centipedim as 8-hydroxyisocoumarin. The UV-spectrum of Centipedin showed maximum absorption at 243 ㎚, and melting point was 122℃. Centipedin was stable at room temperature for three months in appropriate buffers.
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