유리체 출혈을 동반한 망막색소변성 1예

정인영,허현도,김성재,한용섭,서성욱,박종문,In Young Chung,Hyoun Do Huh,Seong Jae Kim,Yong Seop Han,Seong Wook Seo,Jong Moon Park 대한안과학회 2013 대한안과학회지 Vol.54 No.8

Purpose: To report a case of a young male patient with retinitis pigmentosa (RP) accompanied by vitritis and neovascularization of the optic disk in both eyes who underwent unilateral vitrectomy for the treatment of vitreous hemorrhage in the right eye. Case summary: An 8-year-old boy visited our clinic with a complaint of night blindness. Both eyes showed inflammatory cells in the anterior vitreous and neovascularization of the optic disk confirmed by fluorescein angiography. Extensive vitreous hemorrhage developed in his right eye and he underwent unilateral vitrectomy. His final visual acuity was 0.6 in both eyes. Conclusions: Vitreous hemorrhage may be related to chronic inflammation in the vitreous and is a very rare RP complication. Vitrectomy can be an effective treatment option for RP complicated by vitreous hemorrhage.

ESS를 이용한 발전소 터빈제어밸브 전개 운전 제어로직 제안

정인영,이재헌,In Young Chung,Jae-Heon Lee 한국플랜트학회 2023 플랜트 저널 Vol.18 No.4

In order to respond to the demand for flexible operation of thermal power generation, development of natural sliding pressure operation that minimizes throttle loss by opening the turbine control valve 100% and maximize power generation efficiency in conjunction with ESS in order to quickly respond to fluctuations in the system frequency is required. The logic development of natural sliding pressure operation with ESS was developed to modify the existing logic at the power plant's top-level control logic such as the unit master, the boiler master and the turbine master. Cooperative control algorithms that complement the advantages and disadvantages of ESS operation (quick response, limited capacity) and power plant operation (slow response, continuous operation) not only improve efficiency when applied to actual power plants, but also respond quickly and flexibly to load demands to ensure system stability.

메모리 친수성 아크릴릭 인공수정체의 백내장 수술 2년후 임상결과

김유리,서성욱,정인영,송준경,Eu Rie Kim,Seong Wook Seo,In Young Chung,Jun Kyong Song 대한안과학회 2007 대한안과학회지 Vol.48 No.3

Purpose: To evaluate the clinical results of thermoplastic, hydrophilic acrylic IOL CV232 (MemoryLens?, CIVA Vision, USA) to those of hydrophobic acrylic IOL AR40e (Sensar?, AMO, USA). Methods: Twenty patients had in-the-bag implantation of a MemoryLens? IOL in 1 eye and a Sensar? in the opposite eye in a randomized fashion after uneventful phacoemulsification. We evaluated the best corrected visual acuity (BCVA) and posterior capsule opacification (PCO) at a mean 30.05 months after surgery. Results: Two years postoperatively, a BCVA of 0.8 or better was found in 65% of the MemoryLens? group and in 60% of the Sensar? group. There was no statistically significant differences between the two groups (p>0.50). PCO developed in 55% in the MemoryLens? group and in 65% of the Sensar? group. Again, no statistically significant differences were found between the two groups (p>0.50). Conclusions: There were no significant differences between the two different intraocular lenses in BCVA or PCO postoperatively.

SOI 소자에서의 바디 전압 안정화를 위한 실리콘 필름 Island 구조

정인영,이종호,박영준,민홍식,Chung, In-Young,Lee, Jong-Ho,Park, Young-June,Min, Hong-Shick 대한전자공학회 1999 電子工學會論文誌, D Vol.d36 No.1

SOI MOSFET에서 바디 전압을 안정시키기 위하여 바디 저항과 콘택 소모면적을 줄이면서도 SOI 고유의 장점을 그대로 유지시키는 IBC(Island Body Contact) 구조를 창안하였다. 이 구조는 여러 MOSFEET 들의 바디를 서로 연결하여 같이 콘택을 형성함으로써 면적의 증가 없이 훌륭한 바디 콘택효과를 갖게 된다. VLSI 소자로서의 그 가능성을 소자 시뮬레이션과 제작된 소자와 회로의 측정실험을 통하여 확인하였다. A new IBC(Island Body Contact) structure is introduced to SOI CMOS VLSI for stabilizing the body potential of the MOSFET without the additional area consumption. The improvement of the body contact effect is achieved by reducing the body resistance and the area is saved as the bodies of the MOSFETs are connected together. Its property as VLSI device is confirmed through the device simulations and the measurement.

준설해사 충전이 바닥보호공의 투수성에 미치는 영향평가

유전용 ( Yoo Jeon Yong ),오영인 ( Oh Young In ),전상옥 ( Jeon Sang-ok ),정인영 ( Chung In-young ) 한국농공학회 2006 한국농공학회 학술대회초록집 Vol.2006 No.-

Usually, the main body of sea dyke is embanked by good quality banking material from in-land. Saemangeum sea dyke is constructed by dredging seabed soil and reclamation technology, however, because the shortage of in-land banking materials and economical benefit. Therefore, the main body of sea dyke by dredged marine sand is placed over the bottom protection layer(rubble and large crushed stone) that has completely different permeability characteristics. Especially, the final closure of sea dyke is most dangerous due to the fast velocity of tidal flow. The final closure section is consisted with vast rubble and heavy stone gabion, therefore the discharge velocity toward the land side of final close section is irregularly and sometime occur the fast discharge velocity. In this study, the seepage model test and field monitoring performed to evaluate permeability characteristics of bottom protection layer filled with dredged marine sand. Based on model test and field monitoring, the bottom protection layer is gradually filled by dredged marine sand during dredging and reclamation period. Also, the coefficient of permeability of bottom protection layer filled with dredged marine sand is 8.0×10^-6~1.0×10^-5m/sec.

Characterization of Melanin-concentrating Hormone from Olive Flounder (Paralichthys olivaceus)

In Young Chung(정인영),Jeong Min Jeon(전정민),Young Hwan Song(송영환) 한국생명과학회 2018 생명과학회지 Vol.28 No.3

멜라닌 농축 호르몬(melanin-concentrating hormone, MCH)은 17개의 아미노산으로 구성된 환형의 시상하부 펩티드로 색소 침착의 조절인자로서 연어에서 처음 분리되었다. 포유동물의 MCH는 19개의 아미노산으로 구성되어 있으며 섭식 및 에너지 항상성을 조절하는데 관여한다. 본 연구에서는 양식넙치의 다양한 조직에서 MCH 유전자의 발현 분포, 멜라닌 함유 세포의 집적, 포유동물 MCH 수용체와 양식넙치 MCH의 상호작용을 조사하였다. Real-time qPCR을 이용하여 뇌, 정소, 난소에서 MCH 유전자의 발현이 나타나는 것을 확인하였고, 수정 후 발달단계에서도 MCH 유전자의 발현을 확인할 수 있었다. 합성된 연어 sMCH, 포유류 hMCH, 양식넙치 fMCH, dN-fMCH, dC-fMCH를 양식 넙치의 표피에 처리했을 때 다양한 농도에 따라 멜라닌 함유 세포의 집적이 다양하게 나타났다. 연어 sMCH, 포유류 hMCH에 비해 양식넙치 fMCH의 멜라닌 함유세포의 집적도가 36~99.85%로 비역가를 나타났으나 양식넙치 dN-fMCH, dC-fMCH를 처리한 경우 양식넙치 fMCH에 비해 높은 농도에서 집적이 나타나고 짧은 시간에 분산되었다. 또한, 인간 MCH 수용체와 쥐 MCH 수용체가 발현된 포유동물의 세포주에 양식넙치 fMCH를 처리하여 각 수용체와 결합하는 것을 확인하였다. 이러한 결과는 어류에서 발현되는 MCH가 포유동물의 MCH와 유사한 구조를 가지고 있어 MCH 수용체에 대한 새로운 리간드로서 제공될 수 있으며, 향후 어류의 MCH 수용체에 확대 적용할 수 있을 것이다. The melanin-concentrating hormone (MCH), a cyclic hypothalamic peptide composed of 17 amino acids, was initially identified in chum salmon (Oncorhynchus keta) as a regulator of pigmentation. Mammalian MCHs are cyclic hypothalamic peptides composed of 19 amino acids that regulate food intake and energy homeostasis. The present study examined not only MCH expression of different tissues but also the melanohore aggregation and intracellular Ca<SUP>2+</SUP> influx of fMCH and the other MCH. Real-time qPCR showed that MCH expressed specially in the brain, gonad, and ovary, and expression of MCH was observed during the developmental stages. In the application of synthetic fMCH and both types of synthetic fMCH, dN-fMCH and dC-fMCH, scale melanophore induced significant changes in aggregation activity with various concentrations of MCH. Also, compared to hMCH and sMCH, fMCH exhibited a 36~99.85% increase in relative potency (%), whereas aggregation of dN-fMCH and dC-fMCH remained in a high concentration. However, dispersion was induced rapidly according to be low concentration of dN-fMCH and dC-fMCH. We show that fMCH and its derivates were bound human MCHR1 and rat MCHR expressed in HEK293T cells with nano-molar affinity and are likely to be ligand-induced to mobilize intracellular Ca<SUP>2+</SUP>. These results may provide new ligands for binding assay with MCHew ligands, as a structure similar to the mammalian MCH structure was discovered in fish. Once the fMCH receptor system is in place, it can be compared to the MCH system of mammals in terms of MCH function.

Real-time PCR 분석법을 이용한 옥돔과 옥두어의 종 판별법 개발

정인영(In Young Chung),서용배(Yong Bae Seo),양지영(Ji-Young Yang),김군도(Gun-Do Kim) 한국생명과학회 2017 생명과학회지 Vol.27 No.11

미토콘드리아 게놈에 존재하는 시토크롬C 산화효소 서브유닛 I (cytochrome C oxidase subunit I, COI) 유전자의 DNA 염기서열을 기반으로 하는 종 판별은 수산물 자원의 지속적인 개발과 어류 다양성 보존을 위해 폭넓게 적용되고 있다. 본 연구에서는 한국에서 소비되는 옥돔과 가짜 옥돔으로 둔갑하는 옥두어의 종 판별을 위한 분석법을 개발하였다. 옥돔과 옥두어, 두 종의 종 판별과 검증을 위해 미토콘드리아 게놈의 DNA 염기서열 차이를 이용하여 real-time PCR법에 의해 분석하였다. 미토콘드리아 DNA 서열의 생물정복학적 분석에서 옥돔과 형태학적 옥돔 유사종인 옥두어, 두 종 사이에 COI 유전자 내에서 상당히 유사한 DNA 서열 부분과 일부 서열 변화부분이 확인되었다. 명확하게 종 판별을 하기 위해 COI 유전자 내에서 일부 변화된 서열에서 종 특이적 프라이머를 디자인하였다. 10 개체의 옥돔과 옥두어에서 게놈 DNA을 추출하여 옥돔과 옥두어의 종 특이적 프라이머를 이용하여 real-time PCR 시스템에 의해 분석되었다. 이러한 real-time PCR 시스템을 이용한 genomic DNA 기반의 분자 기술은 동물 조직의 분류학적 분류를 위한 신뢰할 수 있는 방법을 제공한다. 옥돔판별을 위해, 옥돔 DNA에서 옥돔 종 특이적 프라이머를 이용한 Ct 평균값(21.85±3.599)과 옥두어 DNA에서 옥돔 종 특이 프라아머를 이용한 Ct 평균값(33.49±1.183) 차이를 나타내었다. 그리고 옥두어판별을 위해, 옥두어 DNA에서 옥두어 종 특이적 프라이머를 이용한 Ct 평균값(22.49±0.908)과 옥돔 DNA에서 옥두어 종 특이 프라아머를 이용한 Ct 평균값(33.93±0.479)을 통해 옥돔과 옥두어의 각 종 특이 프라이머의 효율성, 특이성 및 교차 반응성 측정은 통계적으로 유의한 차이를 보여 주었다. 제안된 방법은 10개의 상용 샘플로 검증이 되었다. 따라서, threshold cycle (Ct) value와 같은 real-time PCR 결과 분석에 의해 종 판별이 가능하였다. DNA barcoding is the identification of a species based on the DNA sequence of a fragment of the cytochrome C oxidase subunit I (COI) gene in the mitochondrial genome. It is widely applied to assist with the sustainable development of fishery-product resources and the protection of fish biodiversity. This study attempted to verify horse-head fish (Branchiostegus japonicus) and fake horse-head fish (Branchiostegus albus) species, which are commonly consumed in Korea. For the validation of the two species, a real-time PCR method was developed based on the species" mitochondrial DNA genome. Inter-species variations in mitochondrial DNA were observed in a bioinformatics analysis of the mitochondrial genomic DNA sequences of the two species. Some highly conserved regions and a few other regions were identified in the mitochondrial COI of the species. In order to test whether variations in the sequences were definitive, primers that targeted the varied regions of COI were designed and applied to amplify the DNA using the real-time PCR system. Threshold-cycle (Ct) range results confirmed that the Ct ranges of the real-time PCR were identical to the expected species of origin. Efficiency, specificity and cross-reactivity assays showed statistically significant differences between the average Ct of B. japonicus DNA (21.85±3.599) and the average Ct of B. albus DNA (33.49±1.183) for confirming B. japonicus. The assays also showed statistically significant differences between the average Ct of B. albus DNA (22.49±0.908) and the average Ct of B. japonicus DNA (33.93±0.479) for confirming B. albus. The methodology was validated by using ten commercial samples. The genomic DNA-based molecular technique that used the real-time PCR was a reliable method for the taxonomic classification of animal tissues.

Cloning and Characterization of BTG-1 Gene from Pacific Oyster (Crassostrea gigas)

In Young Chung(정인영),Jeong Hwan Oh(오정환),Young Hwan Song(송영환) 한국생명과학회 2017 생명과학회지 Vol.27 No.4

BTG1 (B-cell translocation gene 1)은 APRO family (anti-proliferative protein family)에 속하며, 이들은 공통의 생물학적 기능은 세포증식을 억제하는 것으로 알려져 있다. 본 연구에서, 굴의 gill cDNA library를 random sequencing을 통한 EST 분석과정에서 BTG1 clone을 확보하였으며, 분자생물학적 특성을 조사하였다. 굴의 BTG1은 182 개의 아미노산으로 구성되며, zebrafish와 57%, human과 56%의 상동성을 나타냈으며, 사람이나 설치류와 달리 ORF (open reading frame) 내에 intron이 존재하지 않았다. Genomic DNA walking을 통해 굴의 BTG1의 predicted promoter를 확인하였으며, 분석결과 AP-1 element와 SRE (serum response element) 부위가 존재하였으며, 5’flanking region에 cAMP response element (CRE) 부위가 확인되었다. 굴의 BTG1의 조직별 유전자발현 수준을 확인하기 위해 real-time PCR을 수행하였으며, 6 개 조직 모두에서 BTG1의 유전자발현이 나타났으며, 그 중에서 heart와 mantle에서 높은 수준의 mRNA 발현을 확인할 수 있었다. BTG 1 (B-cell translocation gene 1) gene was first identified as a translocation gene in a case of B-cell chronic lympocytic leukemia. BTG1 is a member of the BTG/TOB family with sharing a conserved N-terminal region, which shows anti-proliferation properties and is able to stimulate cell differentiation. In this study, we identified and characterized the pacific oyster Crassostrea gigas BTG1 (cg-BTG1) gene from the gill cDNA library by an Expressed Sequence Tag (EST) analysis and its nucleotide sequence was determined. The cg-BTG1 gene encodes a predicted protein of 182 amino acids with 57% 56% identities to its zebrafish and human counterparts, and is an intron-less gene, which was confirmed by PCR analysis of genomic DNA. Maximal homologies were shown in conserved Box A and B. The deduced amino acid sequence shares high identity with other BTG1 genes of human, rat, mouse and zebrafish. The phylogenic analysis and sequence comparison of cg-BTG1 with other BTG1 were found to be closely related to the BTG1 gene structure. In addition, the predicted promoter region and the different transcription-factor binding site like an activator protein-1 (AP-1) response element involved in negative regulation and serum response element (SRE) were able to be identified by the genomic DNA walking experiment. The quantitative real-time PCR analysis showed that the mRNA of cg-BTG1 gene was expressed in gill, heart, digestive gland, intestine, stomach and mantle. The cg-BTG1 gene was expressed mainly in heart and mantle.

잉어의 생체 내 대사체 변화에 미치는 플록세틴 영향규명 연구

정인영 ( In-young Chung ),박유미 ( Yu-mi Park ),김상민 ( Sang-min Kim ),김일규 ( Il-gyu Kim ),도영선 ( Young-sun Do ),김석만 ( Suhkmann Kim ),한상범 ( Sang Beom Han ),조현덕 ( Hyun-deok Cho ),석광설 ( Kwang-seol Seok ) 한국환경분석학회 2017 환경분석과 독성보건 Vol.20 No.1

The purpose of this study was to clarify the effect of fluoxetine, one of the most frequently prescribed antidepressant drugs, on Cyprinus carpio by comparing the variations in its metabolite concentrations after exposure to the drug, using NMR and LC/Orbitrap MS. Three experimental reactors were employed, and the total experimental period was 14 days. Cyprinus carpio were acclimatized for 3 months in the laboratory and then subjected to the study. One reactor was used as a 14-day control, and the others were exposed to fluoxetine at 10 ng/L and 10 μg/L for 7 days and depurate for 7 days. The variations in metabolite concentration after exposure were analyzed by NMR and LC/Orbitrap MS. Before exposure, the concentration trends of fluoxetine and its main metabolite, norfluoxetine were almost the same in blood and liver samples of Cyprinus carpio. However, after the depuration period, the concentrations of norfluoxetine in the liver and blood samples were higher than those of fluoxetine. The concentration of the remaining norfluoxetine in the liver sample was higher than that in the blood sample. This means that the liver mainly metabolized pharmaceuticals. The main change in the concentrations of metabolites of Cyprinus carpio was shown in amino acids such as leucine, isoleucine, glutamate, tyrosine, and valine; organic acids such as acetate and formate; creatine; and glucose. Although after the depuration period, the control, and 10 ng/L and 10 μg/L exposure groups had overlapping results, the exposure and control groups could be classified clearly after 14 days. These results are expected to contribute to identifying the effects of environmental pollutants by tracing metabolite changes and building a chemical metabolite database.

수동시료채취기를 이용한 대기 및 수계 유기화학물질의 비표적 검색 모니터링 방법 적용성 검토

정인영 ( In-young Chung ),김혁 ( Hyuk Kim ),박유미 ( Yu-mi Park ),김일규 ( Il-gyu Kim ),김상민 ( Sang-min Kim ),강은영 ( Eun-young Kang ),김순지 ( Soon-ji Kim ),이소화 ( So-hwa Lee ),석광설 ( Kwang-seol Seok ),최경희 ( Kyung-hee 한국환경분석학회 2015 환경분석과 독성보건 Vol.18 No.3

The purpose of this study was to compare a few passive samplers that may be used for non-target screening of organic chemicals in air and water. In this study we customized passive sampling and subsequent analytical methods. The methods were applied to three sites (S sites) as potential pollution hotspots and three reference sites (H sites). The number of the chemicals accumulated in polyurethane foam (PUF) in the pollution hotspots was greater than those in the control sites by more than a factor of two. Passive sampling was found to be less affected by the seasonal variations. In S3 site, the results of grab sampling showed significant monthly variations, suggesting the necessity of passive sampling. Up to 90,000 peaks were detected for each site as a result of analyzing of pre-treated samples with two-dimensional gas chromatography time of flight mass spectrometer (2D-GC/TOF-MS) after passive sampling. Especially, there were 800 to 1,800 chemical compounds screened under the condition that the similarity was over 700 and the signal-to-noise ratio was over 1,000. These results suggest that passive samplers would be useful in non-target screening of residual organic chemicals in air and water although further research is needed.