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ICT 원격제어 system 이용 식물진단, Phenomics 연구현황 및 전망
정유진,노일섭,김용권,김회택,강권규,Jung, Yu Jin,Nou, Ill Sup,Kim, Yong Kwon,Kim, Hoy Taek,Kang, Kwon Kyoo 한국식물생명공학회 2016 식물생명공학회지 Vol.43 No.1
Remote sensing는 각종 센서를 이용하여 지표면, 물, 요소 기술에 대해 비접촉, 비파괴적인 방법으로 필요한 정보를 얻어내는 기술이다. 이들 기술은 센서 등의 요소 기술 뿐만 아니라, 센서를 탑재하는 플랫폼과 정보 통신 기술(ICT) 등을 복합적으로 이용한다. 특히 농업 분야에서는 ICT를 중개로 기상이나 토양 등의 환경 정보와 작물 정보를 측정하여 수치화하고 클라우드 컴퓨팅에 의해 생산 단계뿐만 아니라 유통 및 소비 단계까지 관리하는 스마트 농업에 크게 기여한다. 식물을 측정하기 위해서는 비파괴 비접촉 bioimaging (remote imaging)을 포함한 식물기능 remote sensing 기술개발이 필요하다. 또한 식물 과학 분야에서도 유전자 세포 수준에서 개체 수준까지를 대상으로 한 bioimaging 연구가 활발히 진행되고 있다. 최근 들어 표현형 연구를 통해 환경과 유전자형의 관계를 구명하는 phenomics 연구가 활발히 진행되고 있다. 따라서 본 논문에서는 식물기능 원격탐사의 기술동향, 식물진단 및 식물환경응답해석과 식물 phenomics 연구현황에 대해 고찰하였다. Remote Sensing (RS) is a technique to obtain necessary information in a non-contact and non-destructive method by using various sensors on the surface, water or atmospheric phenomena. These techniques combine elements such as sensors, and platform and information communication technology (ICT) for mounting the sensor. ICT has contributed significantly to the success of smart agriculture through quantification and measurement of environmental factors and information such as weather, crop and soil management to distribution and consumption stage, as well as the production stage by the cloud computer. Remote sensing techniques, including non-destructive non-contact bioimaging (remote imaging) is required to measure the plant function. In addition, bioimaging study in plant science is performed at the gene, cellular and individual plant level. Recently, bioimaging technology is considered the latest phenomics that identifies the relationship between the genotype and environment for distinguishing phenotypes. In this review, trends in remote sensing in plants, plants diagnostics and response to environment and status of plants phonemics research were presented.
벼에서 CRISPR/Cas9 활용 고빈도 유전자 편집 방법
정유진,배상수,이긍주,서필준,조용구,강권규,Jung, Yu Jin,Bae, Sangsu,Lee, Geung-Joo,Seo, Pil Joon,Cho, Yong-Gu,Kang, Kwon Kyoo 한국식물생명공학회 2017 식물생명공학회지 Vol.44 No.1
CRISPR/Cas9 기술은 생명공학을 활용한 신품종 작물육성에 있어 패러다임 변혁을 가져다 줄 핵심 기반기술이다. 본 연구에서는 CRISPR/Cas9를 이용하여 유전자편집기술을 기존에 알려진 방법보다 쉽고 정확하게 실험 할 수 있도록 sgRNA 디자인, 벡터구축, 형질전환체 육성 및 분석 등을 자세히 기술하였다. sgRNA는 http://www.rgenome.net/ 사이트에서 NGG 영역을 중심으로 하여 target-up: 5'-ggcaGNNNNNNNNNNNNNNNNNNNN-3'과 target-down: 5'-aaacNNNNNNNNNNNNNNNNNNNNC-3'의 올리고를 디자인하였다. 식물형질전환용 벡터는 pPZP-Cas9-RGEN을 기본으로 하였으며, sgRNA의 프로모터는 OsU3를 이용하여 pPZP::35S::Cas9::PinII-OsU3::sgRNA::Bar-Gen 순으로 구축하였다. 형질전환체의 육성은 단기형질전환 Agrobacterium 법을 사용하였으며 재분화 식물체를 얻는데48일 정도 소요되었다. 형질전환체 유무는 genomic PCR 분석으로 single copy 선발은 TaqMan PCR로 분석하였다. 정밀유전자편집 식물체는 T1 세대에서 T-DNA 삽입되지 않은 식물체를 Bar-strip에 의해 선발하였다. 선발된 식물체의 sgRNA 영역의 염기배열 조사에 의해 유전자 편집 식물체를 육성하였다. 따라서 본 연구에서 CRISPR/Cas9 system에 의한 정밀유전자편집 기술을 이용하여 보다 빠르고 쉽고 경제적으로 유전자가 편집된 개체를 확보할 수 있었다. 본 실험에서 확립된 system은 상업용 식물 계통육성에 이용 가능하여 육종적 가치가 매우 클 것으로 사료된다. The CRISPR/Cas9 is a core technology that can result in a paradigm for breeding new varieties. This study describes in detail the sgRNA design, vector construction, and the development of a transgenic plant and its molecular analysis, and demonstrates how gene editing technology through the CRISPR/Cas9 system can be applied easily and accurately. CRISPR/Cas9 facilitates targeted gene editing through RNA-guided DNA cleavage, followed by cellular DNA repair mechanisms that introduce sequence changes at the site of cleavage. It also allows the generation of heritable-targeted gene mutations and corrections. Here, we present detailed procedures involved in the CRISPR/Cas9 system to acquire faster, easier and more cost-efficient gene edited transgenic rice. The protocol described here establishes the strategies and steps for the selection of targets, design of sgRNA, vector construction, and analysis of the transgenic lines. The same principles can be used to customize the versatile CRISPR/Cas9 system, for application to other plant species.
식물 유성 생식과정에서 후성유전학적 정보해석 및 연구현황
정유진,조용구,강권규,Jung, Yu Jin,Cho, Yong-Gu,Kang, Kwon Kyoo 한국식물생명공학회 2017 식물생명공학회지 Vol.44 No.1
Rapid progress in epigenetic studies has resulted in genome wide information of genetic functions, other than DNA sequence information. However, insufficient understanding and unclear research direction in epigenetics has failed to attract many researchers. Here, we review the sexual reproduction processes that are particularly related to epigenetics in plants. We aim to elucidate the roles of epigenetic information and molecular mechanisms involved in the complex sexual reproduction process of plants, and examine their biological significance.
정유진,김종미,박수철,조용구,강권규,Jung, Yu Jin,Kim, Jong Mi,Park, Soo-Chul,Cho, Yong-Gu,Kang, Kwon Kyoo 한국식물생명공학회 2018 식물생명공학회지 Vol.45 No.4
Although new plant breeding technologies facilitate efficient plant breeding without introducing a transgene, they are creating indistinct boundaries in the regulation of genetically modified organisms (GMOs). The rapid advancement in plant breeding by genome-editing requires the establishment of a new global policy for the new biotechnology, while filling the gap between process-based and product-based GMO in terms of regulations. In this study recent developments in producing major crops using new plant breeding technologies were reviewed, and a regulatory model that takes into account the various methodologies to achieve genetic modifications as well as the resulting types of mutation were proposed. Moreover, the communication process were discussed in order to understand consumers' current situation and problems of new plant breeding technology, establish social acceptance well, and understand consumers' disputes such as GMO crops.
유연성 다중 회귀 모델을 활용한 보행자 이상 행동 예측 모델 연구
정유진,윤용익,Jung, Yu Jin,Yoon, Yong Ik 한국데이터정보과학회 2016 한국데이터정보과학회지 Vol.27 No.1
최근 강력 범죄 및 우발 범죄가 끊이지 않고 있으면서 사회적 불안감이 고조되고 있다. 이에 따라 방범용 카메라, CCTV (Closed Circuit Television)가 범죄 증거 확보와 치안을 위해 사용되고 있다. 그러나 CCTV는 주로 사후 처리 기능으로 사용하고 있으며 사전에 범죄를 예방하기는 힘들다. 본 연구에서는 CCTV로부터 수집된 보행자 데이터를 이용하여 객체의 행동을 분석하고 위험 행동 여부를 추정하기 위한 유연성 다중 회귀 모델을 제안한다. 유연성 다중 회귀 모델은 필터링, 상황분석, 예측 단계로 구성되어 있다. 먼저 보행자에 대한 환경과 상황에 대해 필터링한 후 상황분석에 대한 정보를 구축하고 관찰 객체에 이상 행동이 결정된다. 마지막으로 연관분석을 통해 객체의 행동이 예측되어 위협 상황을 통지한다. 이를 통해 다중 지역에서 객체의 행동을 추적하여 객체 행동의 위험여부를 알 수 있으며, 행동 예측을 통해 범죄 발생을 예측 가능하다. In the recently, violent crime and accidental crime has been generated continuously. Consequently, people anxiety has been heightened. The Closed Circuit Television (CCTV) has been used to ensure the security and evidence for the crimes. However, the video captured from CCTV has being used in the post-processing to apply to the evidence. In this paper, we propose a flexible multi-level models for estimating whether dangerous behavior and the environment and context for pedestrians. The situation analysis builds the knowledge for the pedestrians tracking. Finally, the decision step decides and notifies the threat situation when the behavior observed object is determined to abnormal behavior. Thereby, tracking the behavior of objects in a multi-region, it can be seen that the risk of the object behavior. It can be predicted by the behavior prediction of crime.
멜라닌 및 색소 이상 질환과 장부(臟腑)와의 연관성 고찰
정유진,고우신,윤화정,Jung, Yu-Jin,Ko, Woo-Shin,Yoon, Hwa-Jung 대한한방안이비인후피부과학회 2016 한방안이비인후피부과학회지 Vol.29 No.3
Objectives : The purpose of this research is to understand melanin with both Korean and Western medicine.Methods : We investigated the comprehension of melanin in both western and Korean medicine through literature review and studied relationships between melanin and five viscera(五臟), especially liver(肝), spleen(脾), kidney(腎). We Also studied representative pigmentary disorders(melasma, vitiligo) in western and Korean medicine to figure out how to understand pigmentary disorders in oriental medicine.Results : The results are as follows. 1. Melanin is associate with liver, because free coursing(疎泄) function of liver is the origin of transport melanin to keratinocyte from melanocyte. Also, melanogenesis factors like MITF and CREB are closely associated with liver and pigmentary disorders occur frequently after stress conditions or women. 2. Melanin is absorbed and scattered in keratinocytes by the function of spleen. Pigmentary disorders result from failure of spleen and formation of phlegm-retained fluid(痰飮). 3. Kidney essence(腎精) is the origin of melanin formation. In addition, corticosteroid, the major hormone of melanogenesis is secreted by adrenalin and adrenalin belongs to kidney(腎) in Korean medicine. 4. Melasma is created by disorder of melanin transport and absorbtion, so melasma is associated liver (肝) and spleen(脾). Therefore the treatment for melasma may focus on improvement function of liver and spleen. 5. The destruction of melanocyte or abnormal melanogenesis by disorder of the immune system, metabolic and affective disorders can make vitiligo, so vitiligo is associated with liver and kidney which are major part of melanin formation. Therefore the treatment of vitiligo can focus on improvement function of liver(肝) and kidney(腎).Conclusion : We compared Korean and western medicine to understand melanin. We also interpreted the mechanism of melanin and pigmantary disorders in western medicine and considered the relationship with visceral manifestation theory(臟象論) in traditional Korean medicine. Further studies are needed to apply comprehension of melanin to clinical stage.
정유진,윤화정,고우신,Jung, Yu-Jin,Yoon, Hwa-Jung,Ko, Woo-Shin 대한한방안이비인후피부과학회 2014 한방안이비인후피부과학회지 Vol.27 No.3
Objective : The purpose of this report is to know the effect of Korean medical treatments on exfloliative dermatitis. Methods : We treated a 25-years-old man patient with exfloliative dermatitis on the limbs and back with Korean medicine. After Korean medical teatment for 26 days, we measured the decrease of dermatitis and recovery of skin exfloliate. We recorded pictures of changes on symptoms. Result & Conclusion : The symptoms of exfloliative dermatitis were significantly improved. The dry skin and itching were disappeared and skin damages were almost recovered. Thus Korean medical treatments are effective on exfloliative dermatitis.
정유진,류호진,조용구,강권규,Jung, Yu Jin,Ryu, Ho Jin,Cho, Yong-Gu,Kang, Kwon Kyoo 한국식물생명공학회 2016 식물생명공학회지 Vol.43 No.4
NGS 기술은 전체 게놈 시퀀싱 및 reference 게놈에 alignment에 의해 돌연변이 표현형에 관련된 돌연변이 식별에 이용한다. 그러나 품종 및 계통들을 resequence 하였을 경우 기존의 reference 게놈에 구조적 변이가 보이며, reference와 맞지 않는 게놈지역에서 돌연변이들은 단순한 alignment로 찾을 수 없다. 본 리뷰에서는 NGS 기술을 이용하여 돌연변이체로부터 변이 관련 유전자를 식별하는 MutMap, MutMap-Gap 및 MutMap+ 방법을 기술하였고 지금까지의 연구현황에 대해 기술하였다. 아울러 이들 방법은 nucleotide-binding site-leucine rich repeat (NBS-LRR) 그룹들의 병 저항성 유전자와 같이 구조적 변이를 가진 유전자를 분리하는 등 유용성에 대해 고찰하였다. Next-generation sequencing allows the identification of mutations responsible for mutant phenotypes by whole-genome resequencing and alignment to a reference genome. However, when the resequenced cultivar/line displays significant structural variation from the reference genome, mutations in the genome regions absent in the reference cannot be identified by simple alignment. In this review, we report the current status and prospects in identification of genes in mutant phenotypes, by using the methods MutMap, MutMap-Gap, and MutMap+. These methods delineate a candidate region harboring a mutation of interest, followed by de novo assembly, alignment, and identification of the mutation within genome gaps. These methods are likely to prove useful for cloning genes that exhibit significant structural variations, such as disease resistance genes of the nucleotide-binding site-leucine rich repeat (NBS-LRR) class.
식물의 감수분열에서 상동 재조합 효소 특이 기능의 연구현황 및 전망
정유진,남기홍,김태성,이인혜,조용구,강권규,Jung, Yu Jin,Nam, Ki Hong,Kim, Tae Sung,Lee, In Hae,Cho, Yong-Gu,Kang, Kwon Kyoo 한국식물생명공학회 2018 식물생명공학회지 Vol.45 No.1
Meiosis is a specialized cell division, essential in most reproducing organisms to halve the number of chromosomes, thereby enabling the restoration of ploidy levels during fertilization. A key step in meiosis is homologous recombination, which promotes homologous pairing and generates crossovers (COs) to connect homologous chromosomes until their separation at anaphase I. These CO sites, seen cytologically as chiasmata, represent a reciprocal exchange of genetic information between two homologous non-sister chromatids. RAD51, the eukaryotic homolog of the bacterial RecA recombinase, plays a central role in homologous recombination (HR) in yeast and animals. Loss of RAD51 function causes lethality in the flowering plant, Arabidopsis thaliana, suggesting that RAD51 has a meiotic stage-specific function that is different from homologous pairing activity.
제주산 파인애플 유래 Bromelain관련 유전자 (BL1)를 이용반 형질전환 상추의 특성
정유진,김기훈,최장선,이순열,노일섭,박진희,강권규,Jung, Yu-Jin,Kim, Gi-Hyun,Choi, Jang-Sun,Lee, Soon-Youl,Nou, Il-Sup,Park, Jin-Heui,Kang, Kwon-Kyoo 한국식물생명공학회 2006 식물생명공학회지 Vol.33 No.1
파인애플 (Ananas comosus) 줄기에서 얻어지는 bromelain은 단백질 분해효소 중 cysteine protease의 복합체로 알려져 있다. 본 연구에서는 제주산 파인애플 줄기를 이용하여 bromelain 관련 유전자를 분리하였다. 분리된 BL1 유전자는 총 933개의 염기서열로 311개의 아미노산을 coding 하였다. 지금 까지 알려진 식물 유래 bromelain 관련 유전자와의 alignment 분석한 결과 BAA21929 유전자와 94%, T10516 유전자와 93% 및 P14518 유전자와 81%의 상동성을 보였다. BL1 유전자를 상추 게놈내에 도입하고자 NPTII 유전자 와 BL1 유전자로 제작한 pBI 121 BL 벡터를 Agrobacterium tumefacience LBA4404에 도입한 후, 상추잎 절편에 감염시켜 embryogenic callus 및 재분화 식물체를 육성하였다. 이들식물체로부터 T1세대를 육성하여 PCR 분석을 통해 왜래유전자의 도입 여부를 확인하였다. 또한 형질전환체의 발현여부는 Nothern blot분석 및 eno protease활성을 통해 형질전환체에서 BL1유전자가 안정적으로 상추세포내에서 발현되고 있음을 확인하였다. 따라서 본 실험에서 육성된 bromelain 관련 BL1 유전자가 도입한 형질전환 상추를 육종소재르 활용한다면 상업적으로 유용한 단백질을 분해하는 가수분해효소로써 건강 보조제, 사료첨가제 등에 널리 사용할 수 있을 것으로 생각되어진다. To clarify the roles of bromelain in plants, we isolated BL1 gene encoding bromelain from pineapple stem tissues and sequenced. The full length cDNA is 933 bp and encodes a polypeptide of 311 amino acid residues. The cDNA is most similar 94% at the amino acid level to bromelain previously isolated from pineapple (BAA21929). Explants of Lactuca sativa were co-cultivated with Agrobacterium tume-faciences LBA 4404 strains containing nptII and BL1 gene for transformation. Through initial selection of regenerated explants by culturing on a kanamycin and carbenicillin containing MS medium, multiple shoots were obtained after 2 months of culture. For a complementary step of selection, putative transgenic shoots were transferred to 1/2 Ms basal medium supplemented with 100 mg/L kanamycin and 500 mg/L carbenicillin. The selected shoots were obtained T1 generation seeds with emasculation, and tested with PCR analysis using 35S promoter and BL1 specific primers whether BL1 gene was introduced to genome of the plants. These results confirmed that produced the specific PCR bands in the putative transgenic lines. Additionally the Northern blot and endo protease activity showed that transcripts of BL1 gene were detected in transgenic lines. Theses results suggest that BL1 gene be successfully integrated and transcripted in the transgenic lettuce plants.