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자가면역글로불린 G 측정을 위한 표면탄성파 바이오센서에 대한 연구
김기범 ( Gi Beum Kim ),정우석 ( Woo Suk Cheong ),박영란 ( Young Ran Park ),김상진 ( Shang Jin Kim ),김성종 ( Seong Jong Kim ),강형섭 ( Hyung Suk Kang ),김진상 ( Jin Shang Kim ),홍철운 ( Chul Un Hong ) 한국화학공학회 2011 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.49 No.2
In this study, we have developed shear horizontal(SH) surface acoustic wave(SAW) sensors for the detection of immunoglobulin G(IgG) on the gold coated delay line of SH-SAW devices. As the result of the experiment, we could uniformly immobilize anti-MIgG(mouse IgG) conjugate on the surface of gold. When displaying results of immobilization on the surface of gold using G-anti MIgG conjugate and blocking buffer in frequency shift, G-anti MIgG conjugate showed frequency shift of 75.1 kHz in the initial frequency, and blocking buffer showed frequency shift of 215.7 kHz. When various concentrations of MIgG was added in 100 MHz type sensor, the sensor showed 46.3, 127.45, 161.21 and 262.39 kHz frequency shift at 25, 50, 75 and 100 μg MIgG concentration, respectively.