금강 하구의 Vibrio cholerae non - 01 과 Vibrio mimicus 의 생태

장수현,송대진,양송주,신일식,김영만 ( Soo Hyun Chang,Dae Jin Song,Song Ju Yang,Il Shik Shin,Young Man Kim ) 한국수산과학회 1995 한국수산과학회지 Vol.28 No.1

To study ecological properties of Vibrio cholerae non-O1 and Vibrio mimicus which have been described as new food poisoning bacteria recently, the influence of factors such as temperature, salinity, pH and chemical oxygen demand (COD) on detection rate and density of these bacteria were evaluated. Fifty four seawater samples and 49 bottom deposit samples from estuary of Kum river from March 26th, 1993 to February 22nd, 1994 were used for this study. The detection rate of V. cholerae non-O1 were 16.7 for seawater and 10.2 for bottom deposit, respectively. The total detection rate of V. cholerae non-O1 (11.7%) was a little higher than V. mimicus (10.7%.) Both V. cholerae non-O1 and V. mimicus were mainly detected in estuary water of which showed temperature 24℃ above and salinity 10‰ below. These bacteria were also detected in bottom deposit on January when the water temperature was 3.5℃. From these results, we supposed that temperature, salinity and organic material were important factors to growth of V. cholerae non-O1 and V. mimicus. V. cholerae non-O1 might be grown better than V. mimicus under the fluctuating aquatic environmental condition such as salinity.

Vibrio cholerae non - O1 과 Vibrio mimicus 의 생잔에 대한 염도와 온도의 영향

장수현,송대진,양송주,신일식,김영만 ( Soo Hyun Chang,Dae Jin Song,Song Ju Yang,Il Shik Shin,Young Man Kim ) 한국수산과학회 1995 한국수산과학회지 Vol.28 No.1

Vibrio cholerae non-O1 and Vibrio mimicus, food poisoning bacteria, have detected frequently in fresh water and brackish water. To estabilsh prevention measures of food poisoning outbreak by these bacteria, the adaptability and population changes were examined in fresh water, brackish water (10‰ NaCl) and seawater (30‰ NaCl). Both species poorly survived as temperature increased regardless of water types employed. However, survival time was the shortest in fresh water and longest in seawater at 4℃. In case of brackish water, the bacteria survived best at 15℃ and population were varied only in small numbers. Any significant difference was not observed to both species with respect to water types and temperatures except V. mimicus survived about 5∼6 days longer in brackish water. In conclusion, V. cholerae non-O1 and V. mimicus were not likely to be recovered in normal fresh water, brackish water and seawater, and both biological and physicochemical factors could affect survival of these species.

[화학] 음용수 중 수은 연속자동측정시스템의 개발에 관한 연구

장수현(Soo-Hyun Chang),김효진(Hyo-Jin Kim),김선태(Sun-Tae Kim),김영만(Young-Man Kim) 한국분석과학회 1999 분석과학 Vol.12 No.4

미제방죽중의 Vibrio cholerae non-01과 Vibrio mimicus의 계절적 분포에 관한 연구

장수현(Soo-Hyun Chang) 군산대학교 수산과학연구소 2004 군산대학교 수산과학연구소 연구논문집 Vol.4 No.-

Recently, the sanitary importance of food poisoning bacteria was reported in many aterature. The environmental factors such as temperature, pH, salinity and organic material Nere reported as important factors in determining detection rate and density of these bacteria. Thus, experimental study were conducted in order to evaluate the influence of environmental factors on detection rate and density of these bacteria. Fifty four fresh water samples and 55 bottom deposit samples from Mije pond in Kunsan from June 1996 to May 1997 were used for this study. The detection rate of Vibrio cholerae non-Ol were 50.9% for fresh water and 47.3% for bottom deposit and those of Vibrio mimicus were 34.5% and 38.2%, respectively. The total detection rate of Vibrio cholerae non-Ol (49.1%) was higher than that of Vibrio mimicus (36.4%). Vibrio cholerae non-Ol were only detected in fresh water and bottom deposit from May to October when the water temperature was 20℃ above but Vibrio mimicus were detected in fresh water and bottom deposit from July to September when water temperature was 25℃ above. Vibrio cholerae non-Ol and Vibrio mimicus were detected even in bottom deposit on the February when the water temperature was 6.2℃ but these bacteria were not detcted in fresh water in cold weather. From these results. we supposed that temperature and organic material in aquatic environmental condition were one of the most important factors in determining detection rate and density of Vibrio cholerae non-01 and Vibrio mimicus.

미제방중중의 Vibrio cholerae non-O1과 Vibrio mimicus의 계절적 분포에 관한 연구

장수현(Soo-Hyun Chang) 군산대학교 수산과학연구소 1998 水産科學硏究 Vol.- No.14

Recently, the sanitary importance of food poisoning bacteria was reported in many literature. The environmental factors such as temperature. pH, salinity and organic material were reported as important factors in determining detection rate and density of these bacteria. Thus, experimental study were conducted in order to evaluate the influence of environmental factors on detection rate and density of these bacteria. Fifty four fresh water samples and 55 bottom deposit samples from Mije pond in Kunsan from June 1996 to May 1997 were used for this study. The detection rate of Vibrio cholerae non-O1 were 50.9% for fresh water and 47.3% for bottom deposit and those of Vibrio mimicus were 34.5% and 38.2%, respectively. The total detection rate of 'Vibrio cholerae non-O1 (49.1%) was higher than that of Vibrio mimicus (36.4%), Vibrio cholerae non-O1 were only detected in fresh Water and bottom deposit from May to October when the water temperature was 20℃ above but Vibrio mimicus were detected in fresh water and bottom deposit from July to September when water temperature was 25℃ above. Vibrio cholerae non-O1 and Vibrio mimicus were detected even in bottom deposit .on the February when the water temperature was 6.2℃ but these bacteria were not deteted in fresh water in cold weather. From these results, we supposed that temperature and organic material in aquatic environmental condition were one of the most important factors in determining detection rate and density of Vibrio cholerae non-O1 and Vibrio mimicus.

[화학] 근적외선 분광분석법을 이용한 음주측정기술 개발에 관한 연구

장수현(Soo-Hyun Chang),조창희(Chang-Hee Cho),우영아(Young-Ah Woo),김효진(Hyo-Jin Kim),김영만(Young-Man Kim),이강붕(Kang-Boong Lee),김영운(Young-Woon Kim),박성우(Sung-Woo Park) 한국분석과학회 1999 분석과학 Vol.12 No.5

어패류의 NAG-Nibrio 분포에 관한 연구

장수현(Soo-Hyun Chang) 군산대학교 수산과학연구소 2004 군산대학교 수산과학연구소 연구논문집 Vol.4 No.-

Investigation has been conducted on the distribution of NAG Vibrio in fish and shell-fish, marine invertebrate from May, 1998 to October, 1998. 91 samples of fish including Japan seabass, Lacteolabrax japanicus, Bastard halibut, Paralichthys olivaceus, Spottybelly greenling, Hexagrammos agrammus and 74 samples of shell-fish including Common orient clam, Meretrix lamarckii deshayes, Baby clam, Ruditapes philippinanum and 33 samples of marine invertebrate including Webfoot octopus, Octopus ocellatus, Blue crab, Portunus trituberculatus has been taken at the market place in Kunsan, Korea. The results are as follows; 1. V. cholerae non-O1 was detected in 25 samples out of 193 samples and V. mimicus was detected in 23 samples out of 198 samples. Thus, detection rate of V. choleroe nm-O1 and V. mimicus was 12.6% and 11.6%, respectively. 2. Detection rate of V. cholerae non-Ol was 11.0% in fish samples, 12.2% in shell-fish samples, 182% in marine invertebrate samples. In other hands, detection rate of V. mimirus was 11.0% in fish samples, 10.8% in shell-fish samples, 15.2% in marine invertebrate samples. Thus, it can be concluded that marine invertebrates including Webfoot octopus, Odopus ocellatus, Blue crab, Portunus trituberadatus are very susceptible to V. cholera? non-Ol and V. mimirus comparing fish and shell-fish. 3. Detetion rate of V. cholerae non-Ol was 29.2% in September, 28.6% in August, and 20.2% in July. In other hands, none of V. cholerae non-O1 was detected in March. Detection rate of V. mimicus was 28.6% in August, 2:5.0% in September and 24.006 in July. However V. mimicus was not detected at all in March. 4. MPN values of V. cholerae non-O1 and V. mimicus was 130-l,400 MPN/100g and 33~950 MPN/100g, respectively. It means that the MPN value of V. cholerae non-O1 was higher than that of V. mimicus.

어패류의 NAG-Vibrio 분포에 관한 연구

장수현(Soo-Hyun Chang) 군산대학교 수산과학연구소 2000 水産科學硏究 Vol.- No.16

Investigation has been conducted on the distribution of NAG Vibrio in fish and shell-fish, marine invertebrate from May, 1998 to October, 1998. 91 samples of fish including Japan seabass,Lacteolabrax japanicus, Bastard halibut, Paralichthys olivaceus, Spottybelly greenling,Hexagrammos agrammus and 74 samples of shell-fish including Common orient clam, Meretrixlamarckii deshayes, Baby clam, Ruditapes philippinarum and 33 samples of marine invertebrate including Webfoot octopus, Octopus scellatus. Blue crab. Portunus trituberculatus has been taken at the market place in Kunsaa Korea. The results are as follows; 1. V. cholerae non-Ol was detected in 25 samples out of 198 samples and V. mimicus was detected in 23 samples out of 198 samples. Thus, detection rate of V.cholerae non-Ol and V.mimicus was 12.6% and 11.6%,respectively 2.Detection rate of V. cholerae non-O1 was 11.0% in fish samples, 12.2% in shell-fish samples, 18.2% in marine invertebrate samples. In other hands, detection rate of V. mimicus was 11.O% in fish samples, 10.8%In shell-fish samples, 15.2% in marine invertebrate samples. Thus, it can be concluded that marineinvertebrates including Webfoot octopus. Octopus ocellatus, Blue crab, Portunus trituberculatus are verysusceptible to V. cholerae non-O1 and V.mimicus comparing fish and shell-fish. 3. Detection rate of V. cholerae non-O1 was 29.2% in September, 28.6% in August, and 20.2% in July. Inother hands, none of V. cholerae non-01 was detected in March. Detection rate of V. mimicus was 28.6%in August, 25.0% in September and 24.0% in July. However V.mimicus was not detected at all in March. 4. MPN vulues of V.cholerae non-01 and V. mimicus was 130~l,400 MPN/100g and 33∼950 NPN/100g, respectively. It means that the MPN value of V.cholerae non-01 was higher than that of V.mimicus.

중간광고 도입 찬반에 영향을 미치는 수용자 인식 분석

조창환(Chang-Hoan Cho),김주연(Ju Yeon Kim),오신영(Shinyoung Oh),박경인(Kyung In Park),장수현(Soo Hyun Chang) SBS 2014 미디어경제와 문화 Vol.12 No.4

본 연구는 일반광고와 중간광고의 인식 차이를 비교하고, 중간광고 도입 찬성의견이 형성되는 과정을 구조적으로 살펴보았다. 중간광고 도입의 긍정적 측면과 부정적 측면이 활발히 논의되고 있어, 중간광고 도입에 대한 수용자의 인식을 연구할 시점이라고 생각한다. 따라서 중간광고 도입 찬반의견 형성에 영향을 미치는 요인을 기술수용 모델을 적용하여 분석하였다. 일반광고와 중간광고에 대한 수용자의 인식 차이를 비교분석한 결과, 중간광고에 대한 수용자의 인식은 전반적으로 부정적인 경향을 보이고 있음을 알 수 있다. 연구모형 분석 결과, 중간광고의 가치 등 인지하고 있는 중간광고의 유용성, 중간광고 체계와 효과에 대한 수용자의 지식수준은 중간광고 태도를 매개로 하여 중간광고 도입 찬반에 영향을 미치는 것으로 나타났다. Adoption of commercial-break advertising in network TV is a controversial issue that has both positive and negative aspects, and it is a timely and important subject to study in the current media environment in S. Korea. In this vein, this study compared consumer perceptions of general advertising and commercial-break advertising and examined the structural causal model of consumer"s agreement of commercial-break ads in network television. More specifically, this study examined the factors influencing consumer agreement of commercial-break ads in network TV. The results of a consumer survey showed that consumers perceive commercial-break ads more negatively than general advertising. Structural research model of this study demonstrated that perceived advertising values and level of media system understanding (advertising-supported free media content) influenced consumer"s attitude toward commercial-break advertising, which in turn affected consumer agreement to adoption of commercial-break advertising in network TV.

명태 단백질로 제조한 가식성 필름의 물성 개선

목종수(Jong Soo Mok),송기철(Ki Cheol Song),강창수(Chang Su Kang),장수현(Soo Hyun Chang) 한국수산과학회 2002 한국수산과학회지 Vol.35 No.4

명태 단백질을 이용하여 제조한 가식성 필름의 물성을 개선하기 위하여 필름 제조시 가소제 및 가교제 첨가, 그리고 이중필름에 의한 인장강도, 신장률 및 수분 투과도 등의 영향을 검토하였다. 가소제 첨가에 따른 가식성 필름의 인장강도는 PG, sorbitol, PEG 200 및 glycerol의 순으로 높았고, 신장률은 glycerol, sorbitol, PEG 200 및 PG의 순으로 높았으며, 수분투과도는 PG, sorbitol, glycerol 및 PEG 200의 순으로 낮았다. 또한 가소제의 농도가 증가함에 따라 필름의 인장강도는 감소하고 신장률은 증가하는 경향을 나타내었다. PG와 PEG 200은 신장률에 큰 영향을 미치지 못하여 명태단백질 필름의 가소제로 적절하지 못하였으며, 인장강도와 신장률이 서로 상반되는 glycerol과 sorbitol을 조합함으로써 필름의 물성을 조절할 수 있었다. 한편, 필름은 상대습도가 높을수록 인장강도는 감소하고 신장률과 평형수분함량은 증가하는 경향을 나타내었다. Ascorbic acid, citric acid 및 succinic acid 둥의 가교제를 첨가하여 제조한 필름이 가교제 비첨가 필름에 비하여 인장강도와 수분투과도는 증가하고 신장률은 감소하는 경향을 나타내었으며, ascorbic acid는 0.2%, citric acid는 0.1%, succinic acid는 0.1%첨가하는 것이 바람직하였다. 명태 단백질과 옥수수 단백질로 제조한 이중필름은 명태 단백질단독으로 제조한 필름에 비하여 인장강도를 2배 이상 향상시켰고, 수분투과도를 약 20∼30% 감소시켰다. 한편, 산소투과도는 두 필름간에 차이는 없었으나, polyethylene film에 비하여는 10배 정도 산소차단효과가 우수하였다. 색도를 보면 명태와 옥수수 단백질을 이용하여 제조한 이중필름이 명태 단백질 단독으로 제조한 필름에 비하여 L값과 a값은 낮고 b값과 ΔE값은 높았다. The edible films were prepared from the protein of alaska pollack, Tberagra chalcogramma. Effects of plasticizer, cross linker and laminated film on physical properties such as tensile strength (TS), elongation (E) and water vapor permeability (WVP) of films were investigated. In adding various kinds of plasticizers, TS of the films prepared with propylene glycol (PG) was the highest, and followed sorbitol, polyethylene glycol 200 (PEG 200) and glycerol. Elongation of the films prepared with glycerol was the highest, then sorbitol, PEG 200 and PG. WVP of films showed lower in order of PG, sorbitol, glycerol and PEG 200. TS decreased with the increment of plasticizer concentration, but elongation increased. The addition of both PG and PEG 200 effected weakly on elongation, so they were inadequate as plasticizer for the film. Mixtures of glycerol and sorbitol, which showed opposing both TS and elongation in the films, could control the physical properties of the films. With increasing relative humidity, TS decreased, while elongation and equilibrium moisture content increased. By adding the cross linkers such as ascorbic acid, citric acid and succinic acid, TS and WVP of films increased, while elongation decreased. Ascorbic add, citric acid, succinic acid were most effective for TS at 0.2, 0.1 and 0.1%, respectively. Laminated film with alaska pollack protein and corn zein improved TS above two times, reduced WVP about 20~30%, as compared with the film from alaska pollack protein. Two films did not show the difference to oxygen permeability, but they showed about tenfold greater oxygen resistance than polyethylene film. Laminated film showed higher b and △E value of color difference, lower a and L value than the film from alaska pollack protein.