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Chae Shin Lim(임채신) 한국생명과학회 2009 생명과학회지 Vol.19 No.5
고랭지 파프리카 재배 초기에 기형과 및 단위결과 과실이 많이 발생하는데 야간 저온이 화분 활력 및 생식기관 발달에 미치는 영향을 알아보고자 본 연구를 수행하였다. 주간 28℃ 동일 조건에서 야간온도를 13℃ (LNT)와 20℃ (ONT)로 달리 처리하여 생장상에서 파프리카(Capsicum annuum cv. Plenty)를 재배하였다. ONT와 LNT에서 화분을 채취하여 10, 15, 20, 25, 30℃에서 발아시켰다. 24시간 후 화분의 활력을 살펴 본 결과, 처리에 관계 없이 25℃에서 화분 활력이 가장 좋았고 다음이 30℃였다. 25℃에서 발아율은 ONT화분이 42%, LNT 화분이 21%였다. 화분관신장길이는 화분발아 온도에 관계 없이 ONT화분이 가장 길었다. 화분관 신장 속도는 25℃ 에서 발아시킨ONT화분이 가장 빨랐다. 파프리카가 재배된 ONT와 LNT 조건에서 화분 활력의 차이를 알아보기 위해 배지에 화분을 치상 후 ONT와 LNT에서 화분발아 시험을 실시하였다. LNT에서는 야간(13℃) 동안 화분이 발아되지 않았지만 주간(28℃)조건으로 전환되자 화분이 발아되기 시작하였다. 하지만 ONT에서는 야간(20℃) 초기부터 화분이 발아되기 시작하여 주간(28℃)까지 계속 발아가 진행되었다. LNT에서는 과병길이, 자방지름, 수술길이, 화중 및 과중이 증가하였다. 종자수는 처리간 차이가 없었다. LNT처리구에서도 종자가 잘 형성되었던 것은 주간에 수분수정이 이루어졌기 때문인 것으로 보여지지만 기형과나 단위결과 발생 방지를 위해서는 화분발아를 저해하는 저온범위에 대한 정밀한 연구가 수행되어야 할 것으로 보여진다. Bell pepper plants (Capsicum annuum cv. Plenty) were grown under low night temperatures (LNT: day/night temperature of 28/13℃) and optimum night temperatures (ONT: day/night temperature of 28/20℃) in growth chambers. Pollen grains were collected from plants in the growth chamber and incubated at 10, 15, 20, 25, and 30℃. After 24 hr incubation, in both ONT and LNT, the highest percent pollen germinations were observed at 25℃ followed by germinations at 30℃. Percent pollen germination at 25℃ was 42% in ONT - two times higher than in LNT at 21%. Pollen tube length was much longer at ONT than at LNT, regardless of incubation temperature. Compared with other treatments, earlier and quicker pollen tube elongation was observed in ONT pollen grains incubated at 25℃. To find pollen viability in plant growing conditions, pollen grains were incubated in LNT (28/13℃) and ONT (28/20℃) growth chambers for 24 hr. Petri-dishes with pollen grains were put in the growth chambers at the beginning of the night condition. Pollen grains in the LNT growth chamber did not germinate at night (13℃), but began to germinate when the day condition (28℃) started. Pollen grains in the ONT condition, however, started germinating from the early night (20℃) and germination continued during the day (28℃). Plants in LNT showed increased flower stalk length, ovary diameter, stamen length, flower weight, and fruit length. LNT conditions did not impair seed set. There were no differences in seed sets between fruits at LNT and ONT. Normal seed sets in LNT show that fertilization may be completed during daytime. However, further investigation is needed to find what extent of temperature stress causes malformed and/or parthen℃arpic fruits in this bell pepper.
저온기 시설수박 착과율 증진을 위한 품종 및 화분분리 유기용매 선발
임채신(Chae Shin Lim) (사)한국생물환경조절학회 2010 생물환경조절학회지 Vol.19 No.3
화분량은 ‘금천’ 수박을 제외하고 웅화 l개당 10㎎ 이상이었다. ‘복’ 수박은 1화당 화분무게가 13.8㎎이었고, ‘스피드꿀’ 수박은 12.l㎎으로 겨울철 저온기 재배에 인공수정용 화분생산 품종으로 적당한 것으로 판단된다. 유기용매 침지 후 즉시 건조된 화분발아율은 ‘금천’ 수박이 67%로 가장 낮았고 ‘아폴로꿀’ 수박이 92% 가장 높았다. 유기용매에 15일간 저장했을 경우 ‘복’ 수박 회분의 발아력이 74%로 가장 높았다. 저장 24일 후에는 5품종 모두 25% 이하의 발아율을 나타내었다. ‘금천’과 ‘삼복꿀’ 수박의 화분관 신장속도는 50㎛/hr 미만(저장 24일)으로 다른 품종보다 낮았으며, 발아도중 회분관이 파열되는 경우도 발생되었다. 유기용매 저장 24시간 후 화분발아율은 pentane, ethyl ether, n-hexane, ethyl acetate, acetone에서 각각 45, 39, 34, 23, 19% 순이었고 유기용매 침지시간이 길어질수록 화분활력이 급격히 감소되었다. 화분 표피는 유기용매에 저장한 시간이 길어질수록 장해를 많이 받았는데 유기용매의 종류에 따라 장해정도가 달랐다. ‘복’수박이 화분량, 화분활력, 저장성이 우수하였는데 저온기 착과율 증진을 위한 회분생산 품종으로 보여진다. Poor fruit set during winter period is one of the biggest problem in plastic tunnel watermelon cultivation. Hand pollination is inevitable to maximize fruit set of the winter watermelon. Productivity and viability of pollen grain and organic solvents for pollen storage were investigated. All cultivars produced more than 10 ㎎/flower except for 'Kumchun' cultivar. Pollen amount per flower were 13.8 ㎎ in 'Bok' and 12.1 ㎎ in 'Speedkul'. Germination rate of pollen grains incubated at 30℃ right after soaking in pentane solvent were 76% in 'Kumchun' as the lowest and 92% in 'Apollokul' as the highest. The pollen of 'Bok' showed the highest germination rate by 75% after a 15-day storage in pentane. All cultivars showed their pollen germination rate below 25% after a 24-day storage. Among the cultivars, speed of pollen tube growth in vitro were relatively lower in 'Kumchun' and 'Sambokkul' by below 50 ㎛/hr. Pollen tube of these cultivars tended to burst during its elongation on the medium, Pollen stored 24 hrs in organic solvents showed 45, 39, 34, 23, and 19% of germination in pentane, ethyl ether, n-hexane, ethyl acetate, and acetone, respectively. Compared with light condition, pollen viability was higher in darkness during pollen storage in organic solvents. Pollen grain was susceptible to the organic solvent. The viability of pollen grains seems to be influenced greatly by duration of soaking pollen in organic solvent and the polarity of solvents. Organic solvent damages surface of pollen grain and extent of damage was varied by the solvents.
Chae Shin Lim(임채신),Jeoung Lai Cho(조정래) 한국원예학회 2009 원예과학기술지 Vol.27 No.2
본 연구는 고추속 작물의 저장중 냉해증상을 비교하고자 실시하였다. 착색단고추 ‘쿠프라’와 매운고추 ‘녹광’을 녹숙과 단계에서 수확하여 1, 5, 10℃에 저장하였다. 저장 14일 후 품종에 관계 없이 1℃와 5℃에서 냉해 증상인 피팅이 나타나기 시작했으나 10℃에서는 나타나지 않았다. 두 품종 모두 저장온도가 낮을수록 심한 피팅증상을 보였으나 ‘녹광’이 ‘쿠프라’보다 저온에 더 민감하였다. 품종별 호흡률과 에틸렌 발생률 비교에서 온도에 관계없이 호흡률은 ‘쿠프라’에서 에틸렌 발생은 ‘녹광’에서 많았다. 두 품종 모두 저장 온도가 낮을수록 높은 수분손실과 경도감소를 보였다. 저장온도에 관계없이 저장 21일째 ‘녹광’이 ‘쿠프라’에 비해 수분손실이 4배 많았다. 저장 중 이온누출은 두 품종 모두 온도가 낮을수록 높았다. 위의 결과를 종합해 볼 때 본 실험에 사용된 두 품종에서 저장 중 냉해 증상인 피팅발생은 수분손실, 경도감소, 이온누출과 깊은 관련이 있는 것으로 보여지나 에틸렌발생률과 호흡률과는 관련이 없는 것으로 나타났다. The study was conducted to compare susceptibility of pepper fruits to chilling injury during storage. Sweet ‘Cupra’ and hot ‘Nockgwang’ pepper harvested at the mature-green stage were stored at 1, 5 and 10℃. After a 14-day storage, both cultivars showed surface pitting (SP) symptoms at 1 and 5℃ but not at 10℃. Storing fruits at lower temperature caused a marked increase in SP of both cultivars, but more in ‘Nockgwang’ than in ‘Cupra’. ‘Cupra’ exhibited lower respiration and produced higher ethylene than ‘Nockgwang’ during storage regardless of temperatures. Lower temperature induced higher weight loss (WL) and firmness loss (FL) in both cultivars. Compared with ‘Cupra’, about 4-fold higher WL occurred in ‘Nockgwang’ after a 21-day storage regardless of temperature. During storage, electrolyte leakage (EL) of both cultivars increased at 1 and 5℃, whereas it increased relatively little at 10℃. The SP development at 1 and 5℃ appeared to be associated with higher WL, FL, and EL.
2009 H1N1 인플루엔자 폐렴에서 Procalcitonin의 유용성: 세균성 폐렴과의 감별 역할
안신 ( Shin Ahn ),김원영 ( Won Young Kim ),윤지영 ( Ji Young Yoon ),손창환 ( Chang Hwan Sohn ),서동우 ( Dong Woo Seo ),김성한 ( Sung Han Kim ),홍상범 ( Sang Bum Hong ),임채만 ( Chae Man Lim ),고윤석 ( Youn Suck Koh ),김원 ( Won 대한결핵 및 호흡기학회 2010 Tuberculosis and Respiratory Diseases Vol.68 No.4
Background: Procalcitonin is a well known marker in infection that plays a role in distinguishing between bacterial and viral infections in screening. The aim of the present study was to evaluate the role of procalcitonin in differentiating between 2009 H1N1 influenza pneumonia and community acquired pneumonia of bacterial origin, or mixed bacterial origin and 2009 H1N1 influenza infection. Methods: A retrospective observational study was performed over the 6-month winter period during the 2009 H1N1 influenza pandemic. Ninety-six patient-subjects were enrolled, all of whom had been diagnosed with community acquired pneumonia in emergency department during the study period. On admission, laboratory studies were performed, which included 2009 H1N1 influenza real-time polymerase chain reaction of nasal secretions and procalcitonin on serum; the laboratory values were compared between the study groups. Receiver operating characteristic curve analyses were performed on the resulting data. Results: Compared to those with bacterial or mixed infections (n=62) and bacterial pneumonia with confirmed organisms (n=30), patients with 2009 H1N1 pneumonia (n=34) were significantly more likely to have low procalcitonin levels (p=0.008, 0.001). Using cutoff of value >0.3 ng/mL, the sensitivity and specificity of procalcitonin for detection of patients with confirmed bacterial pneumonia were 76.2% and 60.6%, respectively. A significant difference in procalcitonin was found between 2009 H1N1 pneumonia and pneumonia caused by mixed influenza viral and bacterial infections (0.15 [0.05∼0.84] vs. 10.3 [0.05∼22.87] ng/mL, p=0.045). Conclusion: Serum procalcitonin measurement may assist in the discrimination between pneumonia of bacterial and of 2009 H1N1 influenza origin. High values of procalcitonin suggest that bacterial infection or mixed infection of bacteria and 2009 H1N1 influenza is more likely.