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임종환,장범수,박병권,윤효인,Lim, Jong-hwan,Jang, Beom-su,Park, Byung-kwon,Yun, Hyo-in The Korean Society of Veterinary Science 2003 大韓獸醫學會誌 Vol.43 No.3
The objective of this study was to investigate the residue depletion of cephaalexin in the flounder (Paralichthys olivaceus) after multiple oral administrations and to establish the appropriate withdrawal time for edible tissues. A highly sensitive and specific method for the determination of cephalexin in the serum of flounder by LC/MS was developed and validated. Mean recoveries from serum were 87.2% (ranged from 81.2% to 94.5%) for cepalexin. Recovery and precision met the criteria for the guideline of residual analysis of veterinary drugs by the National Veterinary Research and Quarantine Service (NVRQS) in Korea. The limit of detection and limit of quantitation of cephalexin were 10 ng/ml and 50 ng/ml, respectively. Residual levels of cephalexin in muscle samples were estimated with 95% tolerance limit and 95% confidence to fall below the MRL after a withdrawal time of 4 days and 5 days for the 40 and 160 mg/kg/day, respectively.
Determination of Ceftiofur in Bovine Serum by liquid Chromatography-Electrospray Mass Spectrometry
임종환,장범수,박병권,윤효인,Lim, Jong-hwan,Jang, Beon-su,Park, Byung-kwon,Yun, Hyo-in The Korean Society of Veterinary Science 2003 大韓獸醫學會誌 Vol.43 No.3
This study was aimed to develop a more rapid, simple and sensitive method to determine ceftiofur in bovine serum using LC/MS with electrospray interface. Separation was achieved on the Nova-Pak $C_{18}$ reverse phase column. The mobile phase consisted of 0.1% acetic add in water (A) and acetonitrile (B) and gradiently flowed at the rate of 0.4 mL/min. As a result of analysis of blank muscle samples, matrix interference was not shown. Limit of detection and limit of quantitaion was 5 ng/g and 20 ng/g, respectively. The values of precision and recovery satisfied the guideline of NVRQS. The precision and recovery developed in this method are suitable and sensitive to determine the concentration of ceftiofur in the bovine serum. These results could be applied for the confirmation and quantification in the biofluid.
광릉 활엽수천연림의 산림식생구조, 입지환경 및 탄소저장량
임종환 ( Lim Jong Hwan ),신준환 ( Shin Joon Hwan ),김광택 ( Kim Gwang Taeg ),천정화 ( Chun Jung Hwa ),오정수 ( Oh Jeong Soo ) 한국농림기상학회 2003 한국농림기상학회지 Vol.5 No.2
연구대상지인 광릉시험림은 한반도의 중서부에 위치하고 있으며 온대활엽수림대에 속한다. 광릉시험림 내소리봉(533.1m) 주변에 1 ha(100m × 100m) 면적의 영구조사지아 플럭스 타워를 설치하였으며 KLTER(한국장기생태연구 네트워크) 연구대상지인 동시에 KoFlux DX-site 연구대상지로 지정되어 있다. 영구조사지에 대해 개체목 위치도를 구축하고 임분의 구조와 토양의 물리적, 화학적 특성을 비롯한 임분구성요소(바이오매스, 토양, 낙엽 등)의 탄소수지를 분석하였다. 이 지역에서의 우점수종은 졸참나무, 서어나무이었으며 갈참나무, 까치박달 등이 함께 자라고 있다. 임분조사 결과 흉고직경 2cm 이상인 임목의 밀도는 1,473본/ha 이었고, 바이오매스량은 261.2톤/ha, 흉고단면적은 28.0㎡이었다. 모암은 화강편마암으로 이루어져 있으며 토양형은 갈색산림토양에 속하고, 토심은 38~66cm, 토성은 양토와 사양토, 산도는 표토?에서 pH4.2~5.0, 심토층에서 pH 4.8~5.2로 나타났다. 지료로부터 1.2m 높이에서 촬영한 반구사진(hemispherical photography)을 이용하여 엽면적지수(LAI)의 계절적인 변화를 측정한 결과 최대 LAI가 3.65이었다. 또한 토양수분함량 및 LAI의 공간적인 분포도를 작성하였다. 이 지역에서의 탄소저장량은 지하부를 포함한 임목에 총 136톤/ha, 낙엽층에 약 5.6톤/ha, 토양층 30cm 깊이까지에 약 92.0톤/ha가 저장되어 있는 것으로 분석되어 총 탄소저장량은 236톤/ha인 것으로 나타났다. 이상에서의 현지조사 및 관측 자료는 산림생태계의 구조와 기능 변화를 예측하기 위한 산림동태 모형을 비롯한 생물지화학적 동역학 모델의 적용에 필요한 모수와 자료로 활용될 것으로 판단된다. The study area, Kwangneung Experiment Forest (KEF) is located on the west-central portion of Korean peninsula and belongs to a cool-temperate broadleaved forest zone. At the old-growth deciduous forest near Soribong-peak (533.1 m) in KEF, we have established a 1 ha permanent plot (100 mx100 m) and a flux towel; and the site was registered as a KLTER(Korean long-term ecological research network) and DK site of KoFlux. In this site, we made a stemmap of trees and analyzed forest stand structure and physical and chemical soil characteristics, and estimated carbon budgets by forest components (tree biomass, soils, litter and so on). Dominant tree species were Quercus serrata and Carpinus laxiflora, and accompanied by Q. aliena, Carpinus cordata, and so on. As a result of a field survey of the plot, density of the trees larger than 2 cm in DBH was 1,473 trees per ha, total biomass 261.2 tons/ha, and basal area 28.0 ㎡/ha. Parent rock type is granite gneiss. Soil type is brown forest soil (alfisols in USDA system), and the depth is from 38 to 66 cm. Soil texture is loam or sandy loam, and its pH was from 4.2 to 5.0 in the surface layer, and from 4.8 to 5.2 in the subsurface layer: Seasonal changes in LAI were measured by hemispherical photography at the 1.2 m height, and the maximum was 3.65. And the spatial distributions of volumetric soil moisture contents and LAIs of the plot were measured. The carbon pool in living tree biomass including below ground biomass was 136 tons C/ha, and 5.6 tons C/ha is stored in the litter layer, and about 92.0 tons C/ha in the soil to the 30 cm in depth. Totally more than about 233.6 tons C/ha was stored in DK site. These ground survey and monitoring data will give some important parameters and validation data for the forest dynamics models or biogeochemical dynamics models to predict or interpolate spatially the changes in forest ecosystem structure and function.
무인모선기반 무인잠수정의 3차원 위치계측 기법에 관한 연구
임종환(JONG-HWAN LIM),강철웅(CHUL-UNG KANG),김성근(SUNG-KYUN KIM) 한국해양공학회 2005 韓國海洋工學會誌 Vol.19 No.2
A 3-D localization method of an autonomous underwater vehicle (AUV) has been developed, which can solve the limitations of the conventional localization, such as LBL or SBL that reduces the flexibility and availability of the AUV. The system is composed of a mother ship (small unmanned marine prober) on the surface of the water and an unmanned underwater vehicle in the water. The mother ship is equipped with a digital compass and a GPS for position information, and an extended Kalman filter is used for position estimation. For the localization of the AUV, we used only non-inertial sensors, such as a digital compass, a pressure sensor, a clinometer, and ultrasonic sensors. From the orientation and velocity information, a priori position of the AUV is estimated by applying the dead reckoning method. Based on the extended Kalman filter algorithm, a posteriori position of the AUV is, then, updated by using the distance between the AUV and a mother ship on the surface of the water, together with the depth information from the pressure sensor.
임종환(Jong Hwan Lim),김희정(Hee Jung Kim) 대한기계학회 2017 大韓機械學會論文集A Vol.41 No.6
캠퍼스를 자율적으로 주행하면서 내방객을 안내하는 안내로봇을 개발하였다. 이 로봇은 DGPS와 로봇 바퀴에 부착된 엔코더 정보를 이용하여 자신의 최적 위치를 평가하여 사전에 설정된 안내경로를 자율적으로 주행하면서 안내를 수행한다. 본 연구에서는 안내할 정보를 미리 순서대로 설정하는 기존 방법과 달리 이동 중 자신의 현재 위치를 평가하고 그 위치 주변에 안내할 정보가 있으면 그 정보를 이용하여 안내를 수행하는 위치기반 안내방법을 제안한다. 또한 안내효율을 극대화 할 수 있는 안내시나리오 구성방법을 제시한다. 개발된 안내로봇의 성능을 실제 캠퍼스 안내에 적용하여 평가하였다. A campus guide robot was developed that can autonomously guide people through a university campus. The robot is able to evaluate its location using Differential Global Positioning System (DGPS) and Dead-Reckoning using the encoders mounted on its wheels. The robot can navigate autonomously along a guide route that is set in advance. A new position-based guidance approach was suggested. Unlike the conventional method of setting the guide sequence in advance, the robot acquires guidance by judging whether there is guide information corresponding to its current position. The robot searches guide information from the guide database while it moves along the guide path autonomously. If there is any guide information available around the location of the robot, then it performs guide functions. We also suggested an effective guide scenario that can maximize the interest of people. The performance of the robot was tested through sets of experiments in a true campus environment.