자생 오미자나무의 정유성분 GC/MS 분석

임순성(Soon Sung Lim),이연실(Yeon Sil Lee),한샘(Saem Han),정규환(Kyu Hwan Chung),이상현(Sanghyun Lee),신국현(Kuk Hyun Shin) 한국원예학회 2008 원예과학기술지 Vol.26 No.4

오미자나무로부터 산업적 용도를 찾고자 정유성분을 GC와 GC/MS의 분석법에따라 분석하였다. 오미자나무의 줄기에는 α-pinene(7.46%), β-pinene(30.66%), 1,8-cineole(4.81%), 4-methyl-1-(1-methylethyl)-3-cyclohexene-1-ol(7.90%) 등의 성분함량이 높았고, 오미자나무 뿌리에는 camphene(10.05%), β-pinene(17.67%), sabinene(4.02%), l-limonene(4.04%), 1-methyl-4-(1-methylethyl)benzene(4.97%), linalyl acetate(7.86%), linalool oxide(4.84%) 등의 성분함량이 높았다. 자생 오미자나무 줄기 및 뿌리에 공통적으로 β-pinene의 정유성분이 함유되어 있었으며, 오미자나무 줄기 및 뿌리의 향기특성을 이용한 산업화가 가능하다고 본다. To search for the new development of industrial application, chemical analysis of volatile constituents from native Schizandra chinensis was conducted by GC and GC/MS spectrometric analyses. The chemical composition of the volatile constituents from S. chinensis was confirmed by the aid of Wiley/NBS Library and RI indice searches. The major constituents were α-pinene (7.46%), β-pinene (30.66%), 1,8-cineole (4.81%), and 4-methyl-1-(1-methylethyl)-3-cyclohexene-1-ol (7.90%) from the stems, and camphene (10.05%), β-pinene (17.67%), sabinene (4.02%), l-limonene (4.04%), 1-methyl-4-(1-methylethyl)benzene (4.97%), linalyl acetate (7.86%), and linalool oxide (4.84%) from the roots. Among them, β-pinene is a major constituent in the stems and roots of native S. chinensis that presented possibilities for industrial applications.

꽃사슴 녹용(鹿茸)의 생화학적성분(生化學的成分)의 조성(組成) 및 함량(含量)

임순성,정하숙,백인범,신국현,Lim, Soon-Sung,Chung, Ha-Sook,Baek, In-Bum,Shin, Kuk-Hyun 한국생약학회 1999 생약학회지 Vol.30 No.3

The composition of biochemical components such as lipids, proteins and their amino acid components and inorganic elements in the ashes in unossified antlers from Cervus nippon Temminck var. mantchuricus grown in Korea were analyzed to obtain fundamental data for quality control. As a result, it was found that total lipids were 20.75% which was approximately similar contents with those of proteins (21.8%). Sixteen amino acids were identified from the hydrolysate of the protein fraction. Three gangliosides with very similar TLC patterns of those such as $GM_3$, $GM_1$ and $GM_{1a}$ were identified from the water soluble layer of Folch's partitions. Ash contents were revelaed to be much higher in the sponge layer (40.0%) than in the velvet layer (3.7%).

Hepatoprotective and Antioxidant Capacities of Paecilomyces japonica and Cordyceps sinensis in Rats with CCl₄-Induced Hepatic Injury

Sang Hoon Jung(정상훈),Yeon Sil Lee(이연실),Soon Sung Lim(임순성),Yeong Shik Kim(김영식),Sanghyun Lee(이상현),Kuk Hyun Shin(신국현) 한국원예학회 2009 원예과학기술지 Vol.27 No.4

본 논문은 누에에서 자라는 눈꽃동충하초(Paecilomyces japonica) 자실체와 자연산 동충하초(Cordyceps sinensis)의 간보호효과 및 항산화효과를 연구한 논문이다. 각 동충하초의 70% 에탄올 추출물 모두 DPPH 자유라디칼 소거능을 나타내었으며, 눈꽃동충하초(IC??=163.0㎛ㆍ㎖?¹)가 동충하초(IC??=731.0㎛ㆍ㎖?¹)에 비해 4.5배정도 높은 효능을 나타내었다. 사염화탄소로 유발한 흰쥐의 간손상에서 각 동충하초는 혈청 transaminase를 약하게 감소시켰으며, 300㎛ㆍ㎏?¹의 투여군에서 superoxide dismutase(SOD)와 catalase의 활성을 유의적으로 증가시켰을 뿐만 아니라, thiobarbituric acid(TBA) 반응법으로 측정한 malondialdehyde(MDA)의 양을 유의적으로 감소시켰다. 다른 항산화효소인 glutathione peroxidase(GSH-px)의 활성도 증가시키긴 하였지만, 유의성을 나타내진 않았다. Antioxidant and hepatoprotective activities of the fruiting bodies of Paecilomyces japonica (PJ) grown on silk-worm larvae and of Cordyceps sinensis (CS), a wild form of Cordyceps, were investigated. The 70% ethanol extracts of both fungi, PJ and CS, were found to exhibit free radical scavenging effects on 1,1-diphenyl-2-picrylhydrazyl (DPPH). The inhibitory potency of PJ (IC?? = 163 ㎍ㆍ㎖?¹) was approximately 4.5 folds that of CS (IC?? = 731 ㎍ㆍ㎖?¹). They exhibited a moderate decrease in serum transaminase activities elevated by hepatic damage induced by carbon tetrachloride (CCl₄)-intoxication in rats. Both fungal extracts also exhibited not only significant increases in hepatic superoxide dismutase (SOD) and catalase at a dose of 300 ㎍ㆍ㎏?¹ in CCl₄-intoxicated rats, but also a significant decrease in malondialdehyde (MDA) production in a thiobarbituric acid (TBA) reactant assay in rats. Although no significant glutathione peroxidase (GSH-px) activity was detected, SOD and catalase elevations were obtained.

눈꽃 동충하초의 약물활성

심진영,이연실,임순성,현진이,김성연,이은방,신국현,Shim, Jin-Young,Lee, Yeon-Sil,Lim, Soon-Sung,Hyun, Jin-Ee,Kim, Seung-Yeun,Lee, Eun-Bang,Shin, Kuk-Hyun 한국생약학회 2000 생약학회지 Vol.31 No.2

Cordyceps is reputed for its broad biological activities and as a tonic for replenishing vital function in Chinese traditional medicines. As an attempt to obtain fundamental data for the development of a new type Cordyceps, the effects of the fruiting bodies of cultivated fungus of Paecilomyces japonica grown on silkworm larvae on hyperglycemia induced by streptozotocin(STZ) and by epinephrine in rats and in mice as well as on immunological functions in mice were investigated. The 70% methanol extract of the fungus, when administered orally at 100 and 300 mg/kg in STZ-induced hyperglycemic rats, caused a significant decrease in blood glucose level 3hr after sample treatments. The methanol extract, when administered p.o. at the same dose levels in epinephrine-induced hyperglycemic mice, also caused a significant decrease in serum glucose levels as well as a significant reversal of the liver glycogen contents suggesting its hypoglycemic activity might be due to glycogen breakdown in the liver. Treatment of normoglycaemic mice with the methanol extract of the fungus exhibited a significant glucose tolerance up to 3hr after oral glucose load(2.0 g/kg). The methanol extract also showed immuno-stimulating activity as measured by carbon clearance in mice and a significant antifatigue effect as measured by weight loaded forced swimming performance in mice.

Aloe속 식물의 고분자물질 분획이 알콜대사에 미치는 효과

한용남,정하숙,임순성,이상현,심창섭,신국현,Han, Yong-Nam,Chung, Ha-Sook,Lim, Soon-Sung,Lee, Sang-Hyun,Shim, Chang-Sub,Shin, Kuk-Hyun 한국생약학회 1998 생약학회지 Vol.29 No.2

For the purpose of evaluating protective components against alcohol-induced toxicity, the active components enhancing alcohol metabolism was pursued from water soluble fraction by ethanol precipitation and DEAE-cellulose chromatographic technique. As a result, various high molecular weight fractions from Aloe vera and Aloe arborescens, on a single oral administration in rats were found to cause a significant decrease in the blood ethanol concentration as well as enhancement of liver cytosolic ADH and ALDH activities and among which, a strong acidic high molecular weight fraction was demonstrated to exhibit the most potent enhancing activity on ethanol metabolism.

가시오갈피 줄기의 물 추출물이 지질대사에 미치는 영향

이연실,정상훈,임순성,지준,이상현,신국현,Lee, Yeon-Sil,Jung, Sang-Hoon,Lim, Soon-Sung,Ji, Jun,Lee, Sang-Hyun,Shin, Kuk-Hyun 한국생약학회 2001 생약학회지 Vol.32 No.2

The effects of the water extract from the stem bark of Acanthopanax senticosus were evaluated on hyperlipidemic rats induced by lipid rich diet or poloxamer-407. The water extracts, when administered orally for 3 consecutive days in hyperlipidemic rats induced by poloxamer-407 (1 ml of 30%), was found to cause a significant decrease in plasma cholesterol and triglyceride concentrations. The water extracts, when treated orally for 5 consecutive days also showed a significant inhibition of serum total cholesterol and triglyceride level in rats treated with lipid rich diet (15% cholesterol, 1% sodium cholate and 84% com oil). HDL-cholesterol, however, was increased significantly.

보라우무 메탄올추출물의 HT-29 대장암세포 증식 억제 효과

김은지,박소영,홍지은,신민정,임순성,신현경,윤정한,Kim, Eun-Ji,Park, So-Young,Hong, Ji-Eun,Shin, Min-Jeong,Lim, Soon-Sung,Shin, Hyun-Kyung,YoonPark, Jung-Han 한국식품영양과학회 2007 한국식품영양과학회지 Vol.36 No.4

해조류는 다양한 생리 활성을 나타내는 성분들을 함유하고 있어 항산화효과 등 다양한 생리활성 효과를 나타내는 것으로 보고되고 있다. 본 연구에서는 식용 가능한 28종의 해조류 메탄올추출물 중 보라우무 메탄올추출물이 인간의 대장에서 유래한 암세포인 HT-29세포의 세포증식을 억제함을 밝혔고, 가장 강한 HT-29 세포 증식 억제 효과를 나타낸 보라우무 메탄올추출물의 대장암세포 증식억제 기전을 밝히고자 하였다. 보라우무 메탄올추출물을 HT-29 세포 배양액에 여러 농도$(0{\sim}20{\mu}g/mL)$로 첨가하여 세포를 배양한 경우 보라우무 메탄올추출물 처리 농도가 증가할수록 세포증식이 현저히 감소하였고, apoptotic cell수는 현저히 증가하였다. Apoptosis의 주요한 조절인자인 Bcl-2 family 단백질 중 Bcl-2는 보라우무 메탄올추출물에 의해 변화가 없었고 Bax는 고농도에서만 소폭 감소하였다. 반면, t-Bid 단백질 수준은 보라우무 메탄올추출물의 처리에 의해 현저하게 증가하였다 Bcl-2 family Protein과 더불어 apoptosis의 조절에 주요한 역할을 하는 caspase 단백질의 경우 보라우무메탄올추출물 처리에 의해 cleaved caspase-8, -9, -7, -3 단백질 수준이 현저히 증가하였고, cleaved PARP 단백질수준도 현저히 증가하였다. 또한, caspase-8의 활성화를 유도하는 Fas 단백질 수준도 보라우무 메탄올추출물에 의해 현저하게 증가하였다. 이 결과로부터 보라우무 메탄올추출물은 caspase-8의 활성 증가에 의한 미토콘드리아 막 투과성 변화와 caspase-7/-3 활성의 증가를 통해 apoptosis를 유도함으로써 암세포의 증식을 억제한다는 결론을 내릴 수 있다. 본 연구는 보라우무를 항암효과를 지니는 기능성식품이나 항암제로 개발할 수 있는 가능성을 제시하고 있으며 향후 보라우무를 이용한 제품의 개발을 위해서는 유효성분의 동정 및 그 성분의 작용 기전에 대한 면밀한 추가 연구가 필요할 것으로 보인다. In the present study, twenty eight marine algae species were evaluated for their antiproliferative effect on HT-29 human colon cancer cells. Among these, the methanolic extract of Symphyocladia latiuscula (SL Ex) showed the highest inhibitory activity on HT-29 cell growth. In this study, we examined the mechanism by which SL Ex inhibited the HT-29 cell growth. Cells were cultured with various concentrations of $(0{\sim}20{\mu}g/mL)$ SL Ex. The SL Ex substantially decreased the viable cell numbers and induced apoptosis of HT-29 cells in a dose-dependent manner Western blot analyses of total cell lysates revealed that SL Ex increased the levels of cleaved caspase-8, -9, -7, and -3, and poly (ADP-ribose) polymerase in HT-29 cells. In addition, SL Ex increased truncated Bid levels but moderately decreased Bax levels at only $20{\mu}g/mL$. Furthermore, SL Ex did not affect Bcl-2 protein levels but increased the levels of Fas in HT-29 cells. The present results indicate that SL Ex inhibits cell growth via inducing apoptosis in human colon cancer cells. The mechanism of apoptosis induction by SL Ex involves caspase-8 activation leading to changes in mitochondrial events and subsequent activation of the caspase-7/caspase-3 cascade. Our finding may lead to the development of new therapeutic strategies for the treatment of colon cancer.

토종 복분자와 외래종 복분자 추출물의 항염증효과 비교

양현모(Hyun Mo Yang),임순성(Soon Sung Lim),이연실(Yeon Sil Lee),신현경(Hyun-Kyung Shin),오양석(Yang-Seok Oh),김진경(Jin-Kyung Kim) 한국식품과학회 2007 한국식품과학회지 Vol.39 No.3

본 연구의 목적은 수세기에 걸쳐 발기부전(impotence), 정액루(spermatorrhea), 유뇨증(enuresis), 천식(asthma) 및 알레르기 관련 질병의 치료제로 사용되고 있는 토종 복분자(R. coreanus)씨와 외래종 복분자(R. occidentalis)의 항염증활성을 비교, 분석해 보고자 했다. 그 결과 토종 복분자의 에탄올 추출물이 외래종 복분자의 에탄올 추출물에 비교해 LPS 처리에 의한 NO의 생성을 현저히 억제시키는 것을 관찰 할 수 있었으며, NO와 PGE₂ 생합성효소인 iNOS와 COX-2 단백질의 발현 또한 억제시킴을 확인할 수 있었다. LPS 유도에 의한 TNF-α와 IL-6의 생성억제 효과도 토종 복분자 에탄올 추출물이 외래종 복분자 추출물에 비해 탁월함이 관찰 되었다. 이러한 연구결과로 볼 때 토종 복분자 에탄올 추출물이 macrophage에 의해 생성되는 염증반응의 매개물질인 NO, PGE₂, 염증성 cytokine 등의 생성을 억제함으로 염증반응을 완화시켜 줄 것으로 판단된다. 차후 항염증활성을 갖는 복분자 추출물의 성분을 동정하는 연구가 더욱더 필요할 것으로 사료되며, 토종 복분자가 천연 항염증활성을 갖는 제품 개발에 있어 유용한 식물자원 원료로 사용될 수 있을 것으로 생각된다. The dried fruit of the Rubus coreanus, which is well-known in Korea and referred to as “Bokbunja,” has been employed as a traditional medicine for centuries. This crude drug has been utilized in Korea for the management of impotence, spermatorrhea, enuresis, asthma, and allergic diseases. Our previous study demonstrated that the ethanol extracts of R. coreanus have anti-inflammatory effects. The principal objective of the present study was to conduct a comparison of the anti-inflammatory effects of the ethanol extracts of R. coreanus and R. occidentalis; here, we tested the unripe (URCE), half-ripened (HRCE), and ripened fruits (RCE) of R. coreanus, and the unripe (UROE), half-ripened (HROE), and ripened fruits (ROE) of R. occidentalis. We found that URCE, UROE, HRCE, and HROE reduced the production of nitric oxide and prostaglandin E₂ as well as pro-inflammatory cytokines in lipopolysaccharide-stimulated RAW264.7 murine macrophages. Interestingly, the R. coreanus extracts showed stronger inhibitory effects on the production of these inflammatory mediators than the R. occidentalis extracts.

Expression of Terpenoid Biosynthetic Genes and Accumulation of Chemical Constituents in Valeriana fauriei

Yun Ji Park(박윤지),Soon Sung Lim(임순성),Yeon Bok Kim(김연복),Sang Won Lee(이상원),Sang Un Park(박상언) 한국약용작물학회 2016 한국약용작물학회 학술대회논문집 Vol.2016 No.1

등골나물 추출물의 항염증 효과

이한나(Han Na Lee),임도영(Do Young Lim),임순성(Soon Sung Lim),김종대(Jong Dai Kim),윤정한(Jung Han Yoon Park) 한국식품과학회 2011 한국식품과학회지 Vol.43 No.1

등골나물(Eupatorium japonicum)은 식용으로 이용되어온 식물이지만 생리활성에 대한 연구가 없었다. 따라서 본 연구진은 등골나물 에탄올추출물이 쥐 대식세포인 Raw264.7 세포에 LPS로 유도된 염증 반응에 미치는 영향에 대하여 연구를 수행하였다. 등골나물 꽃 부분에 70% 에탄올을 가하여 얻은 등골나물 에탄올추출물(EJE)을 Raw264.7 세포에 LPS와 함께 0, 1, 2.5, 5, 10 ㎎/ℓ로 처리하여 세포를 배양하였다. LPS에 의해 생성된 NO 및 PGE2 분비는 EJE를 처리함에 따라 감소하였고 이 결과는 EJE 의 독성에 의한 것이 아님이 증명되었다. Raw264.7 세포에 LPS 에 의해 생성된 iNOS, COX-2의 단백질과 mRNA의 발현이 EJE 의 농도 의존적으로 억제되었으며, 염증 반응시 생성되는 IL-6, IL-β, TNF-α의 mRNA 발현도 등골나물 추출물에 의해 현저히 억제되었다. 더욱이 EJE의 분획물 중 EA와 MC 분획물이 독성이 적으면서 효과적으로 NO의 생성을 억제하는 것으로 나타났다. NO 생성 억제효과가 뛰어난 이들 분획물 내의 생리활성 물질에 대한 연구가 추가적으로 수행되어야 한다고 사료된다. 등골나물 꽃을 추출물로 항염증효과를 연구한 논문이 없고 다른 식용 가능한 천연재료들의 항염증효과와 비교하여(data not shown) EJE 분획물의 항염증효과가 낮은 농도에서 탁월한 점을 미루어 볼 때, 등골나물 추출물은 상당히 낮은 농도에서 뛰어난 항염증 물질을 가진 단일물질을 포함할 가능성이 높다고 사료된다. 따라서 위의 결과는 등골나물 추출물이 독성과 부작용이 적은 염증 치료제로 활용될 수 있는 가능성이 높음을 제시한다. Eupatorium japonicum belongs to a family of Asteraceae plants and flowers of E. japonicum have been consumed as a tea. In this study, we investigated whether E. japonicum extract inhibits lipopolysaccharide (LPS)-induced inflammatory responses in Raw264.7 macrophages. The cells were treated with various concentrations (0, 1, 2.5, 5, or 10 ㎎/ℓ) of 70% ethanol extract from E. japonicum flowers (EJE) in Raw264.7 cells. LPS-induced nitric oxide (NO) and prostaglandin E₂ (PGE₂) production were inhibited by EJE up to 67% and 49% of these productions, respectively without any reduction of viable cell numbers. EJE reduced LPS-induced expression of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 proteins and their corresponding mRNA levels. Additionally, EJE decreased the levels of interleukin (IL)-6, IL-1β, and tumor necrosis factor (TNF)-α mRNA. EJE was further fractionated with water, butanol, ethylacetate (EA), hexane, or methylene chloride (MC). Among the resulting five fractions, EA and MC, respectively from EJE significantly inhibited LPS-induced NO production (each inhibition rate was 85.3% of 10 ㎎/ℓ EA fraction and 97.2% of 10 ㎎/ℓ MC fraction) without significant cytotoxicity in Raw264.7 cells. These results indicate that EJE exhibits powerful effects of anti-inflammation and can be developed as a potential anti-inflammatory agent.