Late Cytomegalovirus Disease Causes Ileal Perforation after Kidney trasplantation

이희우,한혜원,박영서,Lee, Hee-Woo,Hahn, Hye-Won,Park, Young-Seo Korean Society of Pediatric Nephrology 2011 Childhood kidney diseases Vol.15 No.1

Cytomegalovirus (CMV)는 신 이식 후 발생하는 감염의 가장 흔한 원인으로, 예방요법과 새로운항 바이러스 제제의 도입에도 불구하고 심각한 결과를 초래하며, 거부반응을 촉진한다는 의견도 제시되고 있다. 저자들은 이식 전 CMV 양성이었던 환아에서 후기 CMV 질환이 발병한 증례를 보고하고자 한다. 이식 6개월 후 mycophenolate mofetil ((MMF)을 투여한 후로 설사와 복통을 호소하던 환아는 이식 후 8개월에 소장이 파열되었다. 환아는 6주간의 치료후에 호전되었다. CMV감염의 위험 인자로 MMF의 역할에 대해서는 더 논의가 필요하나, MMF 투여 시이식 후 바이러스 감염에 대한 주의와 주기적인 추적 관찰이 필요하다. Cytomegalovirus (CMV) is the single most common infection following kidney transplantation and despite prophylactic strategies and the development of new antiviral agents, it still remains a cause of considerable morbidity and mortality. Current literature suggests that CMV infection may trigger rejection. We report a case of late CMV disease in a preemptive seropositive recipient who did not receive CMV prophylaxis. Diarrhea and abdominal cramping persisted after the administration of mycophenolate mofetil (MMF) six months after transplantation and resulted in ileal perforation at eight months after transplantation. The boy recovered after six weeks of treatment with ganciclovir. MMF has been mooted as a risk factor for CMV infection since its introduction, and further investigations are required to confirm its role. More attention to infectious complications is necessary and serial monitoring of viral load is recommended when MMF is administered.

데이터 마이닝을 이용한 서울시교직원의 피로요인 탐색연구

이희우 (Hee Woo Lee),신선미 (Sun Mi Shin) 韓國學校保健學會 2006 韓國學校保健學會誌 Vol.19 No.1

Purpose : To identify general characteristics of school personnel with recent fatigue which was the most frequent symptom among subjective symptoms and to explore fatigue-related factors by evaluating physical and perceived health status, life style, and symptoms through data mining techniques. Methods : We collected a data of the 1,147(male 545, female 602) who were elementary, middle, or high school personnel, answered a questionnaire, and received physical examination in Seoul School Health Center from September to November in 2000. And we investigated the differences between fatigue group and non-fatigue group for demographic characteristics, physical health status, perceived health status, symptoms, and laboratory values by frequency, chi-square test, t-test, or simple logistic regression analysis by SAS package 8.1, and then selected significant variables as input variables of a decision tree analysis of CART model by SAS E-miner. Results : In general characteristics, the fatigue consisted of 41.1%(male 35.2%, female 46.4%) among 1,147 school personnel. In classical statistics, factors related with fatigue were female, lower means of systolic and diastolic pressure, young age, personnel in middle school, irregular eating habit, no exercise a week or less than 30minutes exercise a day, perception of unhealthy status, and subjective symptoms including short of breath at exercise. In simple logistic regression to examine the relationship between selected independent variables and fatigue as a dependent variable, the odds ratio of gender (female vs male) was 1.58 times, and young age ( 20s vs 60s) 20.67 times, and middle vs high school personnel 1.86 times. However, we mined combined several characteristics by SAS-E miner. In CART model, if health perception was healthy, and age was >= 37.5 years, the proportion of the fatigue was only 19.3%. but if health perception was not healthy and symptom was severe 'short of breath' during exercise and age was < 53.5 years, and BMI was >= 22.69, the proportion of the fatigue was up to 84.8%. Conclusions : The fatigue consisted of 41.1%(male 35.2%, female 46.4%). In classical statistics, fatigue-related factors among school personnel were young age, female gender, perceived unhealthy status, subjective physical symptoms, poor life-style, and lower blood pressure rather than only physical health status. However, in data mining, if health perception was healthy and age was >= 37.5 years, the proportion of the fatigue was only 19.3%. but if health perception was not healthy and symptom was severe 'short of breath' during exercise and age was < 53.5 years, and BMI was >= 22.69, the proportion of the fatigue was up to 84.8%.

무인비행장치용 측량 및 관측용 탑재 카메라의 최적화 조건 연구

이희우 ( Hee-woo Lee ),손호웅 ( Ho-woong Shon ),김태훈 ( Tae-hoon Kim ) 한국산업융합학회 2023 한국산업융합학회 논문집 Vol.26 No.6

Unmanned aerial vehicles (UAVs, drones) are becoming increasingly useful in a variety of fields. Advances in UAV and camera technology have made it possible to equip them with ultra-high resolution sensors and capture images at low altitudes, which has improved the reliability and classification accuracy of object identification on the ground. The distinctive contribution of this study is the derivation of sensorspecific performance metrics (GRD/GSD), which shows that as the GSD increases with altitude, the GRD value also increases. In this study, we identified the characteristics of various onboard sensors and analysed the image quality (discrimination resolution) of aerial photography results using UAVs, and calculated the shooting conditions to obtain the discrimination resolution required for reading ground objects.

“Joined-Delta Wing”형 다목적 무인기 플랫폼 개발

이희우(Hee Woo Lee),최봉묵(Bong Muk Choi),김재흔(Jae Heun Kim),오웅택(Ung Tack Oh) 대한기계학회 2015 대한기계학회 춘추학술대회 Vol.IT융합 No.-

지방유래 중간엽줄기세포의 신경세포로의 전이분화 능력과 신경계 질환 치료에서 적용 가능성

이희우 ( Hee-woo Lee ),김준순 ( Jun-soon Kim ),임우석 ( Woo Seok Im ),성정순 ( Jung-joon Sung ) 서울대학교 인구의학연구소 2015 人口醫學硏究論集 Vol.28 No.-

The nervous system is essential for normal physiological function of all systems within the human body, and a complex collection of nerves and specialized cells known as neurons that transmit signals between different parts of the body. However, the nervous system has a limited capacity for self-repair. Recently, stem cell therapy using easily accessible mesenchymal stem cells, has come to as a potential therapeutic regime. Adipose tissue-derived mesenchymal stem cells (ADSCs) have the capacity to differentiate into endodermal and ectodermal cell lineage in vitro. Thus, it is important that not only is it a prerequisite to understand the functional properties of the differentiated cells, but also deciphering the regulatory pathways and mechanisms of the ADSCs themselves, suggesting that it will expedite the development and usage of ADSCs for autologous stem cell therapy in treating neurological diseases. We discuss the neural differentiation potential of human ADSCs and potential implication of these cells in treating neurodegenerative diseases.

CRISPR/Cas 지놈 편집 기술을 이용한 빠른 세대전환 유전자 조작 생쥐 생성

이희우 ( Hee Woo Lee ),성정준 ( Jung Joon Sung ),김혜민 ( Hye Min Kim ),이재삼 ( Jae Sam Lee ) 서울대학교 인구의학연구소 2014 人口醫學硏究論集 Vol.27 No.-

지난 30년 동안, 유전자 변형 마우스는 생체 내에서 유전자의 기능 연구에 매우 중요한 도구가 되었다. 생쥐의 지놈 변이를 위해 배아줄기세포내에서 유전자표적화 방법은 인간 질병에 중요한 정보를 제공해왔으며 약물 발견 및 표적 검증을 위한 플랫폼 역할을 해왔다. 최극에 널리 알려진 유전자편집 방법중 clustered regularly interepaced short palindromic repears(CRISPRs) and CRISPR-associated (Cas)[CRISPR/Cas]기반의 유전자편집은 생쥐와 쥐 등에 정확한 유전적 변화를 유도하기 위한 새로운 기술로 각광 받고 있다. 인간질병 모델로서 유전자 조작 마우스의 중요성을 감안 할 때. CRISPR은 유전자 변이를 통해 줄기세포와 체세포의 질병 관련 유전자를 제거하거나 교정하는 새로운 치료법 개발에 매우 유용할 것이다. 따라서 본 종설에서는 새로운 동물모델 생성에 있어 매우 혁신적인 장을 마련 할 수 있고, 또한 의생명과학 연구를 촉직 할 수 있는 가능성을 충분히 가진 CRISPR/Cas 유전자편집에 대하여 자세히 살펴보고자 한다.

용액중합에 의해 제조된 폴리아닐린 / 폴리에틸렌옥사이드 블렌드의 전기전도도 특성에 관한 연구

이동규(Lee Dong Kyu),차국헌(Cha Kook Heon),이희우(Lee Hee Woo),김진환(Kim Jin Hwan) 한국고분자학회 2000 폴리머 Vol.24 No.1

국내 남해안에 발생한 적조원인생물들의 24S rRNA 유전자 염기서열분석

이수웅,이희우,박종규,이진애,박영식,Lee, Soo-Woong,Lee, Hee-Woo,Park, Jong-Gyu,Lee, Jin-Ae,Park, Young-Shik 한국해양학회 1998 바다 Vol.3 No.2

국내 남해안의 적조발생지역에서 채집한 Prorocentrum minimum, P. micans, P. triestinum, P. balticum, Gymnodinium sanguineum, Alexandrium catenella, Scrippsiella trochoidea, Heterosigma akashiwo를 순수배양하고 이로부터 PCR을 이용해 약 700 bp에 해당되는 24S rRNA 유전자의 D1과 D2 변이부위를 증폭하고 클로닝한 후 염기서열을 분석하였다. 분석된 염기서열들을 이미 밝혀진 적조원인종들의 것과 ClustalW 프로그램을 사용하여 배열하고 계통수를 구성한 결과 Prorocentrum과 Alexandrium 속의 것들은 형태적인 분류결과와 종수준까지 거의 일치함을 보여주었다. 이러한 연구결과는 그 24S rRNA 유전자의 염기서열분석이 국내에서 발생하는 적조원인종들에 대한 종 수준에서의 신속한 확인에 이용될 수 있음을 제시한다. Cultured isolates of Prorocentrum minimum, P. micans, P. triestinum, P. balticum, Gymnodinium sanguineum, Alexandrium catenella, Scrippsiella trochoidea, and Heterosigma akashiwo from red tides in southern coast of Korea were phylogenetically compared on the basis of their 24S rRNA genes. For each isolate approximately 700 bp of 24S rDNA, encompassing D1 and D2 hypervariable domains, was amplified using the polymerase chain reaction and sequenced. The sequences were aligned with those reported in Genbank by using ClustalW program and phylogenetic tree was generated to show that morphospecies designations in the Alexandrium and Prorocentrum species are congruent with terminal taxa defined by phylogenetic analysis of the 24S rRNA fragment. Our results suggest that the molecular phylogeny approach could facilitate identification of domestic dinoflagellates which have ambiguous morphologies.

생물막형성 장내세균의 Curli 및 Cellulose 세포외 바탕질 분석

최예환,이희우,김성민,이제철,이유철,설성용,조동택,김정민,Choi, Yeh-Wan,Lee, Hee-Woo,Kim, Sung-Min,Lee, Je-Chul,Lee, Yoo-Chul,Seol, Sung-Yong,Cho, Dong-Taek,Kim, Jung-Min 한국미생물학회 2011 미생물학회지 Vol.47 No.4

본 연구에서는 병원 감염의 주요 원인균으로 생물막 관련 감염증에서 흔히 분리되고 있는 Citrobacter, Enterobacter 및 Serratia 등의 임상분리 장내세균 22주를 대상으로 생물막 형성능을 조사하고, 생물막의 세포외 바탕질의 구성 성분을 알아보기 위하여 Congo-red 한천배지상의 집락 형상과 calcofluor 염색을 시행하였다. 또한 curli 생성 오페론인 csgBA(C) 유전자의 유무 확인과 csgA 유전자의 염기서열을 규명하였다. $37^{\circ}C$에서 24시간 배양 후 생물막 형성능 분석에서는 2주의 S. marcescens를 제외한 나머지 20주는 모두 생물막 형성능이 있는 것으로, $28^{\circ}C$에서 48시간 배양 후 분석에서는 22주 모두 생물막 형성능이 있는 것으로 확인되었다. Congo-red 한천배지에서의 균집락 형상을 조사한 결과 균속에 따라서 균 집락의 표현형상이 다르게 나타났으며, 동일 균속내에서도 균 집락의 표현형상과 색 농도의 차이가 관찰되어 세포외 바탕질의 주요 성분 및 생성량의 차이가 있음을 시사하였다. 1주의 C. freundii와 4주의 E. cloacae에서 csgBA(C) 유전자 양성을 나타내었다. 염기서열 분석결과, E. cloacae의 csgA는 E. coli의 csgA와 80.9%, E. sakazakii의 csgA와 75.7%, 그리고 C. freundii의 csgA와 67.8%의 상동성이 있는 것으로 나타났다. E. cloacae의 경우 Congo-red 염색의 결과와 curli 유전자 검색 결과가 일치하였을 뿐만 아니라, csgA 유전자를 보유하지 않은 균주의 생물막 형성능이 csgA 유전자를 보유한 균주에 비해 현저하게 낮은 것으로 나타나, curli가 E. cloacae의 생물막 세포외 바탕질 구성성분의 주요 요소일 것으로 추정되었다. In this study, 22 clinical isolates of Enterobacteriaceae including Citrobacterfreundii (6 strains), Enterobacter cloacae (5 strains), Enterobacter aerogenes (3 strains), Serratia marcescens (7 strains) and Pantoea spp. (1 strain) were investigated for the biofilm forming ability and biosynthesis of curli and cellulose. Biofilm forming ability was the highest among the isolates of E. cloacae and the lowest among the isolates of E. aerogenes. The expression of the biofilm-forming extracellular matrix components, cellulose and curli fimbriae, was examined by Congo-red (CR) staining and calcofluor staining methods. PCR screening for the presence of curli gene (csgA) revealed that 4 strains of E. cloacae and 1 strain of C. freundii carried the csgA, showing a good correlation between the phenotypic detection of curli fimbriae by CR staining method and the genotypic detection of curli gene by PCR in E. cloacae.

유기인 화합물 측정용 광바이오센서에서 AChE-viologen 복합 LB막 제작조건의 최적화

최정우(Jeong-Woo Choi),민준홍(Junhong Min),정태성(Tae-sung Jeong),이희우(Hee Woo Rhee),이원홍(Won Hong Lee) 한국생물공학회 1997 KSBB Journal Vol.12 No.5