화장품 소재로서의 흑마늘 추출물에 대한 안전성 평가

이현순(Hyun-Sun Lee),김선희(Seon-Hee Kim) 한국식품영양과학회 2010 한국식품영양과학회지 Vol.39 No.8

본 연구는 흑마늘을 기능성 화장품 소재로 활용하기 위하여 그 기능성을 in vitro에서 tyrosinase 및 elastase 저해활성을 측정한 결과 피부노화에 관여하는 두 효소의 활성을 모두 저해하였다. 흑마늘 추출물을 hartley계 기니픽 수컷을 사용하여 피부 1차 자극 실험을 실시한 결과 1차 피부자극지수(P.I.I.)가 0.23으로 practically non-irritation(비자극성)에 해당하는 자극으로 피부 자극이 거의 없었음을 알 수 있었다. Maximization test법으로 피부 감작성을 확인한 결과 시험물질에 의한 홍반과 부종 등이 전혀 유발되지 않았다. 이상의 결과로부터 흑마늘 추출물에 대한 기니픽의 피부 감작율은 0%로, 피부에 대한 피부 감작성이 없는 것으로 확인되었다. 따라서 이러한 본 연구의 결과는 흑마늘 추출물이 기능성 화장품 소재로 피부노화를 억제하는 기능성과 안전성 확보된 소재임을 추정할 수 있었다. We evaluated the anti-aging potential and safety of black garlic extract for cosmeceutical ingredient. Black garlic was made by spontaneous fermentation for 40 days at 60~70℃, 85~95% RH without any additives. The 10% black garlic extract had sweet odor, antioxidant activities and inhibitory activities of skin againg enzymes such as tyrosinase and elastase. The skin safety was performed to evaluate of potential toxicity using the primary irritation test and skin sensitization test. The black garlic extract did not show any adverse reactions such as erythema and edema on intact skin sites at primary irritation test, but on abraded sites, some experimental animals showed very slight erythema. So, the black garlic extract was classified as a practically non-irritating material based on the score 0.23 of primary irritation index. The skin sensitization study was tested by the guinea pig maximization test (GPMT) and Freund's complete adjuvant (FCA) with intradermal injection of 10% black garlic extract. The skin sensitization test showed no skin sensitization. The allergic sensitization depends on tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). The concentration of IL-6 on challenged tissue of treated with black garlic extract was not significantly different with negative control group (saline treated group). Based on this study, the potential for black garlic as a cosmeceutical ingredient was proven.

구름버섯 알칼리 추출물에서 분리한 항응고성 다당류

이현순(Hyun-Sun Lee),권미향(Mee-Hyang Kweon),임왕진(Wang-Jin Lim),성하진(Ha-Chin Sung),양한철(Han-Chul Yang) 한국식품과학회 1997 한국식품과학회지 Vol.29 No.2

고령친화식품의 정책 및 산업기술 동향

이현순(Hyun-Sun Lee),남영주(Young-Joo Nam),김양은(Yang-Eun Kim),김종찬(Jong-Chan Kim),신윤정(YunJeong Shin),이영진(YoungJin Lee),허완(Wan Heo) 한국식품과학회 2020 식품과학과 산업 Vol.53 No.4

Korea has entered into an aged-society in 2018. If this trend continues to increase, it is expected that a superaging society will take place by 2026. Therefore, Korea is placed in the situation of becoming the world’s fastest aging nation as it becomes a super-aging society from an aged-society in twenty-six years. In order to provide an effective supply of nutrition for the socially weak, Japan developed a variety of foods with food texture improvements. Germany improved accessibility for meal service development. Senior-friendly food is a softened food or a highly concentrated drink, which is considered a texture-modified food, for the elderly with eating disorders to digest food more easily by making food particles smaller. Varying food processing techniques such as freeze-thawing enzyme impregnation, high-pressure processing, super-heated steam processing, 3D food print, and others used to produce texture-modified foods.

품종별 들깻잎 열수추출물의 caffeic acid와 rosmarinic acid의 함량 및 항산화력 비교

이현순(Hyun-Sun Lee),이현아(Hyun Ah Lee),홍충의(Chung-Oui Hong),양성용(Sung Yong Yang),홍성유(Sung Yu Hong),박상률(Sang Yul Park),이호정(Ho Jung Lee),이광원(Kwang-Won Lee) 한국식품과학회 2009 한국식품과학회지 Vol.41 No.3

본 연구는 품종별 들깻잎의 기능성 건강식품에 항산화력 측정과 생리활성물질인 caffeic acid와 rosmarinic acid의 함량 분석을 통하여 항산화관련 기능성 식품소재에 적합한 들깻잎의 품종을 찾기 위하여 수행하였다. 분쇄한 각 품종별 들깻잎을 환류 추출하여 여과한 후 동결 건조한 물질을 각 실험의 시료로 사용하였다. 총 폴리페놀 함유량을 측정한 결과, 보라들깨와 동글2호가 각각 69.4 g GAE/kg DM, 61.8 g GAE/kg DM로 높은 수치를 나타내었다. 그리고 항산화 실험 결과, FRAP에서는 보라들깨가 796mM FeSOsub{4}?7H2O/g DM로 가장 높은 항산화력을 가진 것으로 나타났으며, 그 뒤로 동글2호가 748 mM FeSOsub{4}?7H2O/g DM으로 높았고, DPPH 결과에서는 SCsub{50}값이 동글2호가 241 μg DM/mL로 가장 항산화력이 높았고, 그 다음으로 261 μg DM/mL로 보라들깨였다. 플라보노이드 함유량 측정에서는 늘보라들깨가 64.5 g QE/kg DM로 가장 높은 결과를 나타내었다. HPLC를 통하여 이미 잘 알려진 항산화제인 caffeic acid와 rosmarinic acid의 함유량을 측정한 결과, caffeic acid는 보라들깨에서, rosmarinic acid는 동글 2호에서 가장 높은 함유량을 나타내었다. 즉, 이 종합적인 비교를 통하여 항산화능력과 생리활성물질 함유량 측면에서 보라들깨와 동글2호가 가장 적합한 품종이라고 결론지을 수 있다. The principal objective of this investigation was to identify adequate species for the harvest of Perilla frutescens leaves, which provide profound antioxidant activities, and harbor abundant caffeic acid and rosmarinic acid. Namchun, Donggeul-2, Bora, Sae-bora and Neul-bora variants of the plant were assessed herein. In this study, we evaluated the antioxidant effects of these plants, and utilized an HPLC system to verify their caffeic acid and rosmarinic acid contents. Dried Perilla frutescens leaves were boiled at a temperature of 100℃ for three hours, and were lyopholized in a freezedryer. The extracts were then processed in order to confirm their antioxidant activities via 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical-scavenging activity assay, ferric-reducing antioxidant power (FRAP) assay, total flavonoid content and total polyphenol content assays. According to the observed antioxidant results of the five tested species of Perilla frutescens leaves, Bora and Donggeul-2 were shown to have more potent antioxidant activities than the other tested variants. The scavenge 50% of DPPH radical (SCsub{50} of DPPH) values were 241 μg DM/mL in Donggeul-2 and 261 μg DM/mL in Bora. Based on the results of the FRAP assay, the Bora variant showed a value of 796 mM FeSOsub{4}?7Hsub{2}O/g DM, and Donggeul-2 exhibited a value of 748 mM FeSOsub{4}?7Hsub{2}O/g DM, of which the total polyphenol contents were measured as 69.4 g GAE/kg DM and 61.8 g GAE/kg DM, respectively. Moreover, the Bora variant had the highest level of caffeic acid, and Donggeul-2 showed the highest rosmarinic acid content among the tested samples (0.87 mg/100 g wet base and 121 mg/100 g wet base, respectively). According to the results of this experiment, we selected two species, Bora and Donggeul-2, which were both verified to contain adequate and favorable antioxidant activities.

구름버섯 기원 항응고성 다당류의 혈액응고 저해기작

이현순(Hyun-Sun Lee),권미향(Mee-Hyang Kweon),임왕진(Wang-Jin Lim),성하진(Ha-Chin Sung),양한철(Han-Chul Yang) 한국식품과학회 1997 한국식품과학회지 Vol.29 No.4

당뇨 쥐에 대한 한약재복합추출물로 만든 현미율무밥의 혈당강하 효과

이현순 ( Hyeon Sun Lee ),공현주 ( Hyun Joo Kong ),이언희 ( Eon Hee Lee ),황수정 ( Su Jung Hwang ),정현아 ( Hyeon A Jung ),김미림 ( Mi Lim Kim ),최은미 ( Eun Mi Choi ),장정현 ( Joung Hyeon Jang ),양경미 ( Kyung Mi Yang ) 대한본초학회 2014 大韓本草學會誌 Vol.29 No.3

Objectives : The purpose of this study was to evaluate boiled rice made from unpolished rice, Job`s Tears, and extract from medicinal herbs mixture (HGMCJE) for hypoglycemic effect on STZ-induced diabetic rats. Methods : In the response of glucose tolerance, control and diabetic rats groups was intubated with glucose and HGMCJE. Furthermore, boiled 100% polished rice (P rice), boiled unpolished rice mixed with white rice and Job`s tears (polished rice:unpolished rice:Job`s tears=60:25:15, UPJ-rice), and UPJ rice made from HGMCJE (HUPJ-rice) were intubated to control and diabetic rats groups, respectively. Then, blood glucose concentration, incremental blood glucose, and area under the curve (ΔAUC) were analyzed in every experimental groups, and these data were used to evaluate glycemic response. Results : When glucose and p rice were intubated in control and diabetic rats, blood glucose concentration, incremental blood glucose, and incremental response ΔAUC of diabetic groups were significantly increase than control groups. But administraion of a single dose of extract from medicinal herbs mixture and HUPJ-rice in control and diabetic rats inhibited the remarkable increase the level of postprandial blood and ΔAUC at 60, 90, and 120 min Conclusions : These results indicate that when intubation of glucose and P rice were out of control on postprandial glycemic response in diabetic rats. But postprandial glycemic response was well-modulated by administrating a single oral dose of HGMCJE and HUPJ-rice. Therefore, HGMCJE can be developed as an effective hypoglycemic agent.

가자(Terminalia chebula Retz.) 추출물의 간섬유화 억제활성

이현순(Hyun-Sun Lee),구윤창(Yun-Chang Koo),이광원(Kwang-Won Lee) 한국식품과학회 2009 한국식품과학회지 Vol.41 No.5

간성상세포(HSC)는 간섬유화와 간경변에 중요한 역할을 한다고 알려져 있다. 간손상에 의해 둥근 모양의 간성상세포는 활성화되어 세포외기질(ECM)을 생산하는 myofibroblast와 같은 모양으로 활성화 된다. 활성화된 간성상세포의 특징은 빠른 증식 속도와 collagen과 같은 세포외 기질의 생산이다. 활성화된 간성상세포의 제거방법은 apoptosis를 유도하는 것이다. 가자 추출물은 정상 간세포(rat primary hepatocyte), 간세포주(HepG2) 및 활성화된 간성상세포주인 T-HSC/Cl-6에 1,000 μg/mL의 농도까지 처리하여 세포독성을 확인하였다. 그 결과 hepatocyte나 HepG2에서는 최고 농도에서도 독성이 없었으나 T-HSC/Cl-6는 U-shape 모양으로 사멸하는 것을 확인 하였다. T-HSC/Cl-6의 사멸이 apoptosis에 의한 것인지를 Annexin-V/PI double staining을 통하여 확인한 결과 apoptosis에 의해 T-HSC/Cl-6의 사멸이 일어나는 것을 확인하였다. Activation of hepatic stellate cells (HSCs) is known to be responsible for hepatic fibrosis and cirrhosis. When round-shape quiescent HSCs go to activation by liver injury, production of extracellular matrix is increased, and its shape becomes myofibroblast-like shape. The activated HSCs are characterized by the high rate of proliferation and the increased production of extracellular matrix. One way of the regeneration of activated HSCs is an apoptosis induction followed by removing the activated myofibroblast-like cells. The effect of extract of Terminalia chebula Retz. (TCE) on cytotoxicity was evaluated using the rat primary hepatocyte, HepG2 and T-HSC/Cl-6 by incubating these cells with TCE up to the dose of 1,000 μg/mL. At the maximum dose of TCE, no cytotoxicity was found on primary hepatocyte and HepG2, but cytotoxic effect of TCE was found on activated HSCs, and T-HSC/Cl-6 in a U-shaped dose-response manner with the highest effect at 500 μg/mL of TCE. Finally, we confirmed the occurrence of apoptotic cell death by annexin-V/PI double staining. The population of annexin-V positive cells was increased in a dose dependent manner.

피부 주름개선 소재개발을 위한 식용작물의 최종당화산물 생성 억제활성

이현순(Hyun-Sun Lee),윤진아(Jin A Yoon) 한국식품영양과학회 2010 한국식품영양과학회지 Vol.39 No.2

피부의 주름은 노화과정의 결과로 유도되어지는데, 비효소적 glycation 반응은 주름생성의 원인 중에 하나로 단백질의 비가역적 가교결합에 의해 생성된 최종당화산물(AGEs)이라고 불리는 갈색화합물을 생성한다. 비교적 반감기가 긴 collagen은 쉽게 glycation이 일어나고 collagen의 가교결합(cross-linking)이 일어나면서 여러 단계의 반응을 거쳐 최종적으로 생성된 AGEs가 피부에 축적되어 피부의 탄력감소 및 주름을 일으킨다고 알려져 있다. 피부 내에 glycation된 collagen의 축척은 피부의 탄력성을 유지하는 것을 방해 한다. 수종의 식용작물로부터 항주름소재를 개발하기 위하여 메탄올 및 열수추출물을 조제한 후 AGEs 형성을 억제하는 활성을 검색하였다. 먼저 서양향신료 중에서는 월계수잎, 계피, 정향, 오레가노, 로즈마리, 사보리, 팔각과 한약재 중에서는 복분자, 달개비, 삼지구엽초, 가자와 해당화의 메탄올 및 열수추출물에서 최종당화산물 생성 억제활성이 높았다. 그러나 국내 식물 중에서는 단지 참취 및 녹차 추출물의 메탄올과 열수추출물만이 높은 활성을 보였으며 해조류 중에서는 활성이 높은 시료는 없었다. Skin wrinkles typically appear as a result of aging processes. One of causes may be the nonenzymatic glycation followed formation of browning products called advanced glycation endproducts (AGEs), an irreversible cross-linked protein. The accumulation of glycated collagen cross-linked in skin inhibits the formation and function of skin tightening agents such as collagen and elastin. To development for anti-wrinkle ingredients from edible plants, MeOH and hot-water extracts were prepared and evaluated for their inhibitory effects of AGEs formation. The activities of both extracts from bay laurel (Laurus nobilis), cinnamon (Cinnamomum loureirii), clove (Eugenia caryophyllate), oregano (Origanum vulgare), rosemary (Rosemarinus officinalis), savory (Satureja hortensis) and star anis (Illicium verum) of western spices, and blackberry (Rubus coreanus), dayflower (Commelina communis), Epimedium koreamun (whole), termunalia frutus (Terminalia chebula) and turkestan rose (Rosa rugosa) of medicinal plants were higher than the others. Of Korean vegetables, however, MeOH and hot-water extract from only Asters caber and green tea showed higher activities, and no activity in Korean marine plants (seaweeds).

반려동물 건강 기능성 식품과 보충제 관련 시장 현황

이현정(Hyun Jung Lee),이현순(Hyun-sun Lee) 한국식품영양과학회 2022 식품산업과 영양 Vol.27 No.1

UVB로 손상이 유도된 인간 피부 섬유아세포(Hs68)에서 일부 식품소재 에탄올 추출물의 피부 보호 효과

이하나(Hana Lee),이종민(Jong Min Lee),홍성문(Sung Moon Hong),김나영(Na Young Kim),손석준(Seok Jun Son),정영재(Young Jae Jeong),이현순(Hyun-Sun Lee),이준수(Junsoo Lee) 한국식품영양과학회 2018 한국식품영양과학회지 Vol.47 No.6

UVB 조사는 MMPs를 활성화시킴으로써 콜라겐을 감소시키고 결과적으로 주름을 형성한다. 본 연구에서는 식품소재 8종을 선정하여 에탄올 추출물을 제조하고 광노화 억제 효능을 평가하였다. 세포독성과 UVB에 대한 보호 효과는 MTT assay를 이용하여 측정하였다. ROS 발생 정도는 fluorescent spectrophotometer로 측정하였으며 수용성 콜라겐과 MMPs의 생성량은 assay kit을 사용하여 측정하였다. Hs68 세포주를 이용하여 50 μg/mL의 농도로 세포독성을 확인하였으며 시료의 독성은 나타나지 않았다. 레드파프리카, 토마토, 호박, 쥐눈이콩(중국산), 밤껍질 에탄올 추출물은 UVB 자극에 대해 각각 88, 104, 98, 97, 88%의 보호효과를 보였다. 이 추출물들은 UVB 조사로 유도된 ROS의 생성량을 감소시켰으며 콜라겐 생성량을 증가시켰다. 또한, 추출물들은 UVB 조사에 의한 MMP-1과 MMP-3의 증가를 억제했다. 그중 밤껍질이 UVB로 유도된 MMPs의 생성을 가장 효과적으로 감소시켰다. 결론적으로 본 연구를 통해 밤껍질 추출물은 자외선에 의한 피부노화와 주름 생성을 효과적으로 개선할 수 있는 가능성을 가짐으로써 항노화 및 항주름 소재로 화장품에 활용될 수 있을 것으로 기대된다. Ultraviolet B (UVB) irradiation is a major cause of photoaging and leads to the formation of skin wrinkles by degrading collagen via activation of matrix metalloproteinases (MMPs). In this study, we investigated the protective effects of ethanol extracts from selected food materials against UVB-induced photoaging in human skin fibroblast (Hs68) cells. The protective effects of the samples on cell viability were examined by MTT assay under UVB (30 mJ/cm2)-treated and untreated conditions. Intracellular levels of reactive oxygen species (ROS) were determined by using a fluorescent spectrophotometer. The levels of MMPs and collagen were measured by assay kits. The extracts (50 μg/mL) did not show any cytotoxicity. Extracts from red paprika, tomato, pumpkin, chestnut shell, and Jinuni bean showed protective effect against UVB irradiation (88, 104, 98, 97, and 88%, respectively). These extracts reversed elevation of ROS production and prevented reduction of collagen levels. Moreover, the extracts inhibited elevation of MMP-1 and MMP-3 levels caused by exposure to UVB irradiation in Hs68 cells. Among the samples tested, chestnut shell was found to exhibit the strongest inhibitory effect against elevation of UVB-induced MMPs. Taken together, these data suggest that chestnut shell is the most promising food material for treatment of UVB-induced photoaging in cosmetic products.