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녹화 처리기간에 따른 황기, 석결명, 술패랭이꽃 및 질경이 새싹채소의 항산화 효과 변화
이철희,신소림,김나래,윤성은,김수인,백설희,황주광,Lee, Cheol-Hee,Shin, So-Lim,Kim, Na-Rae,Yoon, Sung-Eun,Kim, Su-In,Baek, Seol-Hee,Hwang, Ju-Kwang 한국자원식물학회 2009 한국자원식물학회지 Vol.22 No.4
새로운 항산화 기능성 새싹채소를 개발하고, 항산화효과가 우수한 재배 조건을 구명하기 위하여 황기, 석결명, 술패랭이꽃, 질경이 새싹채소의 재배 중 녹화기간에 따른 항산화효과 변화를 분석하였다. 연구의 결과, 종에 따라 광합성이 항산화능에 미치는 영향이 각기 다르게 나타났다. 황기 새싹채소는 총 폴리페놀 함량은 녹화 0일, 총 플라보노이드 함량은 녹화 3일째에 가장 우수하였으며, DPPH radical 소거능은 녹화 0일, ABTS radical 소거능은 녹화 1일, $Fe^{2+}$ chelating효과는 녹화 0일, 지질과산화 억제활성은 녹화 3일째에 가장 우수하였다. 석결명 새싹채소는 2일 녹화했을 때 항산화물질 함량이 높고 radical 소거능이 우수하였으며, $Fe^{2+}$ chelating효과는 녹화 0일, 지질과산화 억제활성은 녹화 1일째에 가장 우수하였다. 술패랭이 새싹채소는 녹화 3일 째에 항산화물질 함량이 높고, $Fe^{2+}$ chelating 효과가 우수하였으며, radical 소거능과 지질과산화 억제 활성은 녹화시키지 않았을 때 가장 우수하였다. 질경이 새싹채소의 항산화물질 함량 및 radical 소거능은 녹화 2일째에 가장 우수하였으며, $Fe^{2+}$ chelating 효과는 녹화 0일, 지질과산화 억제활성은 녹화 3일째에 가장 우수하였다. 연구의 결과, 식물 종에 목표로 하는 항산화 효과에 따라 광합성이 미치는 영향이 다르므로, 식물 종과 원하는 항산화 효과에 따라 재배 방법을 달리해야 할 것으로 생각되었다. The potential use of 4 plant species, Astragalus membranaceus var. membranaceus, Senna occidentalis, Dianthus longicalyx and Plantago asiatica, as new sprout vegetables with high antioxidant function was examined in the present experiments. Seeds of above plants were allowed to germinate under light condition, and seedlings were maintained under dark condition for shoot growth in length for contain period of time. Then the seedlings were put under light for photosynthesis (greening treatment) for the period of 0${\sim}$3 days. Samples were collected to analyze the changes in antioxidant levels and activity, and it was observed that antioxidant substances were affected by greening treatments, depending on plant species. In A. membranaceus, the contents of total polyphenol was highest with no greening, total flavonoids with 3 days greening, DPPH radical scavenging effects with no greening, ABTS scavenging with 1 day greening, $Fe^{2+}$ chelating effects with no greening, and inhibitory activity against linoleic acid peroxidation with 3 day greening. In S. occidentalis, highest levels of antioxidant activity and radical scavenging effects were obtained by 2 day greening, $Fe^{2+}$ chelating effects by no greening and inhibitory activity against linoleic acid peroxidation by 1 day greening. In D. longicalyx, highest levels of antioxidant activity and $Fe^{2+}$ chelating effects were obtained by 2 day greening, $Fe^{2+}$ chelating effects by no greening and inhibitory activity against linoleic acid peroxidation by 1 day greening. In D. longicalyx, highest levels antioxidant activity and $Fe^{2+}$ chelating effects were observed with 3 day greening, and highest radical scavenging effects and inhibitory activity against linoleic acid peroxidation with no greening treatment. In P. asiatica, antioxidant activity and radicals scavenging effects were highest with 2 day greening, whereas highest chelating effects was obtained with no greening and highset inhibitory activity against linoleic acid peroxidation with 3 day greening. As the length of greening treatments influenced the antioxidant levels and function in plant species tested in this experiments, different culture methods are recommended for different plant species to get maximum health benefits out of sprout vegetables.
프린터 색역에 균등한 분포를 갖는 색표본 생성 및 색재현
이철희,김희수,안석출,하영호,Lee, Cheol-Hee,Kim, Hee-Soo,Ahn, Suk-Chul,Ha, Yeong-Ho 대한전자공학회 2000 電子工學會論文誌-SP (Signal processing) Vol.37 No.5
본 논문에서는 출력 장치의 색역에 대하여 균일한 분포를 갖는 색표본(color sample) 생성 방법을 제안하고, 이를 이용한 색재현 방법을 소개한다. 즉 기존의 방법인 RGB(red, green, and blue) 혹은 CMY(cyan, magenta, yellow) 등 장치 의존형 색공간에서 균일한 색표본을 선택하는 것이 아니라 장치 독립형 균등 색공간인 CIELAB공간에서 균등 색표본을 선택하는 방법을 제안한다 또한 제안된 색표본의 성능을 평가하기 위하여 회귀 모델과 신경망을 이용한 색공간 변환을 수행하였다 동일한 크기의 색표본의 경우, 제안된 색표본을 이용한 색공간 변환 방법이 신경망, 회귀 모델 모두에서 색차를 줄일 수 있었다. This paper proposes a color sample selection method that produces a uniform distribution in the display gamut plus a color reproduction method for using a uniform color sample In contrast to the conventional method, the proposed uniform color samples are selected m CIELAB, a device-independent color space, instead of RGB (red, green, and yellow) or CMY (cyan, magenta, and yellow) space, device-dependent color spaces To evaluate the performance of the proposed color samples, they were applied to color space conversion using both a regression model and neural network As a result, in the case of a color sample of the same size, the color space conversion method using the proposed samples showed a lower color difference for color conversions using either neural or regression.
이철희(Cheol Hee Lee),백기엽(Kee Yeoup Paek),황주광(Ju Kwang Hwang) 한국육종학회 1995 한국육종학회지 Vol.27 No.3
Somatic hybridization between axenic shoot culture of Dendranthema grandiflorum and albino cell suspension of Salpiglossis sinuata was investigated. Out of six fusion protocols and three culture media, electrofusion and KP8 medium were found to be most effective in generating somatic hybrids. Hybrid selection strategy, based on complementation for chlorophyll proficiency by the fusion of a non-dividing mesophyll protoplast system and a cytoplasmic albino mutant protoplast system was effective. Following plant regeneration, the hybridity of the regenerated plants was confirmed by morphological, cytological and biochemical analyses. Morphologically, these hybrids resembled D. grandiflorum. The somatic chromosome numbers of these plants were 2n=60. Leaf peroxidase and esterase isoenzyme analyses showed that the regenerated plants contained the banding patterns of D. grandiflorum, plus two bands associated with S. sinuata in the peroxidase profiles.
이철희 ( Cheol Hee Lee ) 한국현대영어영문학회 2014 현대영어영문학 Vol.58 No.3
Eliot uses Heraclitus`` space in his Four Quartets. Although he uses Heraclitus``s principle only in "Burnt Norton," it is the theme of Four Quartets. But many people tend to overlook this fact. Heraclitus asserts that the center of all things is logos, and all things always change in the center of logos. Also, Eliot begins "Burnt Norton" with an epigraph of Heraclitus``s spatial principle. He defines time as a continuation of past, present, and future. This can be explained only metaphysically, not physically. Using the same way as Heraclitus``s principle, Eliot points to a harmony between antitheses and the harmonious moment, which he calls the still point, just as Aristotle calls it an unmoved mover. Eliot represents the still point as "dance" and "love". In conclusion, Eliot relies on Heraclitus``s spatial principle, and he creates his most meditative philosophical poem, Four Quartets.
자생란 3종의 기내 종자발아에 미치는 배지구성물질의 영향
이철희(Cheol Hee Lee) 한국자원식물학회 2006 한국자원식물학회지 Vol.19 No.2
우리나라에 자생하는 해오라비란(Habenaria radiata), 자란(Bletilla striata), 복주머니란(Cypripedium macranthum)을 실험재료로 하여 종자발아에 영향을 미치는 요인에 대해 연구하였다. 해오라비란의 종자발아는 전반적으로 고체배지에서 좋았으며, Hyponex 고체배지에서 29.6%로 가장 높은 발아율을 보였다. 그러나 발아 후 생육은 MS배지에 코코넛워터 100mL L-1을 첨가한 MSC 액체배지에서 가장 양호하였다. 자란의 종자는 MS 고체배지에서 28.2%로 가장 높은 발아율을 보였다. 반면, 액체배지에서는 배지의 종류에 관계없이 전혀 발아되지 않았다. 복주머니란의 자가 및 타가수분 후 90일 된 종자를 6종류의 배지에 배양한 결과 타가수분한 종자는 배지의 종류에 관계없이 발아가 되었으며, 특히 MSC배지에서 가장 양호한 4.45%의 발아율을 보였다. 그러나 자가수분 된 종자는 모든 종류의 배지에서 전혀 발아가 관찰되지 않았다. This study was carried out to investigate the factors affecting seed germination of Korean native orchids, such as Habenaria radiata, Bletilla striate and Cypripedium macranthum. In general, seeds of H. radiata was effectively germinated on solid medium and the highest germination (29.6%) was achieved on Hyponex solid medium. But shoot growth was better in MS liquid medium containing 100mL L-1 coconut water (MSC medium). Seed germination of B. striata was effectively induced on MS solid medium. However, no seed germination was observed in all liguid medium tested. Seed germination experiments of C. macranthum were performed using 6 kinds of media and seeds harvested 90 days after cross- and self-pollination. Cross-pollinated seeds were germinated on all the media with the best germination ratio of 4.45% on MSC medium. But no germination was observed from self-pollinated seeds.
이철희 ( Cheol Hee Lee ),최옥자 ( Ok Ja Choi ) 한국화상학회 2010 한국화상학회지 Vol.16 No.1
본 논문에서는 쪽을 이용한 천연염색에서 염색의 횟수에 따른 발색 재현 기술을 개발함으로써 반복 염색에 따른 발색 시뮬레이터의 개발에 기여하고자 한다. 천연염료로는 쪽을 사용하였고 염색의 매질로는 닥원료를 사용하였다. 닥원료를 1회에서 70회까지 반복하여 염색한 닥죽을 만들고 이를 제지하여 천연염색된 감지(紺紙)를 제작하였다. 원지로부터 반복 염색된 감지의 발색을 재현하기 위하여 주성분 분석법을 이용하였다. 주성분 분석을 위해 모집단으로는 멘셀색시료를 사용하였으며 RGB카메라를 고려하여 3개의 기저함수로부터 직물의 분광반사율을 모델링하였다. 그리고 닥원료로 만든 원지(原紙)와 염색된 감지의 분광반사율을 기저함수의 선형조합으로 표현하고 이로부터 염색횟수에 따른 감지의 분광 반사율의 변화를 예측하기 위한 감쇄함수를 구하는 방법을 제안하였다. 제안된 방법의 성능평가를 위해 염색 횟수별로 실제 염색한 감지와 원지로부터 예측한 영상을 비교하고 정량적인 색차평가를 수행하였다. 결과 반복 염색시 재현성이 일정하다는 가정에서 색차 1미만의 범위에서 천연염색에 따른 감지의 발색을 재현 할 수 있음을 확인하였다. The purpose of this study is to contribute to develope color modeling simulator for indigo dyeing by producing a method of color reproduction according to iteration number of natural indigo dyeing. Polygonum tinctorium was used as a natural dye and dak(the paper mulberry) was used for dyeing material. Dak gruel obtained from dak was dyed iteratively from 1 time to 70 times using natural indigo from polygonum tinctorium then, sheets of indigo-dyed gamjis were produced by a traditional papermaking from the dyed dak gruel. In order to reproduce color of the gamjis, principal component analysis(PCA) was utilized. As a population for PCA, color patches of Munsell books of color were used and 3 basis functions were calculated to model the surface reflectance of the gamjis to consider a RGB camera in real application. Then, surface reflectance of non-dyed paper, which obtained from the pure dak gruel without dyeing, and those of iteratively dyed gamjis were represented in terms of weighted sum for the 3 basis functions, respectively. From these surface reflectances for both non-dyed paper and real dyed gamjis, the attenuation functions were calculated to estimate the change of surface reflectances of the gamjis by iterative dyeing with indigo and used to estimate surface reflectance of iteratively dyed gamjis from the non-dyed paper. In order to evaluate the performance of the proposed method, color difference between images of real dyed gamjis and their estimated images by the proposed method were compared. As a result, our method could reproduce color of a gamji within 1 △E(*)(ab) (color difference, CIELAB1976) under the assumption that if repetition number of indigo dyeing is same then reproduced colors of gamjis have same surface reflectances in every dyeing process.