지주막하 출혈에 따른 Vasospasm에 대한 Transcranial Doppler의 임상적 적용

이준홍,Lee, Jun Hong 대한임상신경생리학회 1999 Annals of Clinical Neurophysiology Vol.1 No.1

Vasoconstriction of intracerebral arteries is the leading cause of delayed cerebral infarction and mortality following aneurysmal subarachnoid hemorrhage. Transcranial Doppler studies show and increase in the flow velocities of basal cerebral arteries, which usually start around day 4 following a subarachnoid hemorrhage, and peaking by days 7 to 14. Angiographic studies confirm the presence of at least some degree of MCA vasospasm when the flow velocities are higher than 100 cm/sec. Mean velocities in the 120 to 200 cm/sec range correspond to 25 to 50% luminal narrowing. MCA and ACA vsospasm is detected with around 90% specificity. Sensitivity is 80% and 50% respectively. A 200cm/sec threshold and rapid flow velocity increase exceeding 50 cm/sec on consecutive days, has been associated with subsequent infarction. Transcranial Doppler is also used to monitor the effects of endovascular treatment of vasospasm. Flow velocities decrease following successful angioplasty or papaverine infusion. Overall, transcranial Doppler studies are considered to have acceptable accuracy for the evaluation of vasospasm in aneurysmal subarachnoid hemorrhage, with limitations that have to be taken into consideration in the clinical setting.

수질 TMS 시행에 따른 기업체 준비사항과 대응방안

이준홍,Lee, Jun-Hong 환경보전협회 2006 환경정보 Vol.28 No.365

제5차 대한간학회 춘계학술대회 초록집 : 특발성 문맥압 항진증의 임상 양상

이준홍 ( Lee Jun Hong ),강대환 ( Kang Dae Hwan ),이창훈 ( Lee Chang Hun ),송근암 ( Song Geun Am ),조몽 ( Jo Mong ),양웅석 ( Yang Ung Seog ) 대한간학회 1999 Clinical and Molecular Hepatology(대한간학회지) Vol.5 No.1(S)

중심축 하중을 받는 Castellated 합성기둥의 비탄성 좌굴 거동

이준홍(Lee, Jun-Hong),김주우(Kim, Joo-Woo) 한국강구조학회 2020 한국강구조학회 학술대회 발표집 Vol.31 No.1

형상기억합금을 이용한 Castellated 합성기둥의 원상복원 효과

이준홍(Lee, Jun-Hong),김주우(Kim, Joo-Woo) 한국강구조학회 2021 한국강구조학회 학술대회 발표집 Vol.32 No.1

일차 배양된 신경세포에서 capsaicin의 선택적 작용

이원택(Won Taek Lee),이준홍(Jun Hong Lee),이성호(Sung Ho Lee),박경아(Kyung Ah Park),이종은(Jong Eun Lee) 대한해부학회 2002 Anatomy & Cell Biology Vol.35 No.2

Capsaicin, the pungent algesic substance of the red pepper is known to be a neurotoxic substance, interrupting the pain conducting pathway. The neurotoxic effects of capsaicin to adult animals were regarded to be limited to the peripheral nervous system. Several reports suggested that central nervous system could be changed after capsaicin administration to the adult rats. To determine the effect of capsaicin to central nervous system, morphologic changes and biochemical assay were investigated in mouse primary cultured CNS neuron. The susceptability of capsaicin differs throughout different parts of brain areas. Cerebral cortex and hippocampus were more sensitive, while striatum, thalamus and midbrain area were less sensitive to capsaicin. After capsaicin treatment, cortical and hippocampal neurons died in dose-and time-dependent manner. By observation of nuclear fragmentation of capsaicin treated neuron, it is thought that the type of cell death is apoptosis rather than necrosis. The capsaicin receptor- immunoreactive cells were observed in the cerebral cortex and hippocampus. It is consistent with area of damaged neuron. These results demonstrate that capsaicin induced the apoptosis through acting with capsaicin receptors; calcium influx due to activation of capsaicin receptor may induce apoptosis. 붉은 고추에서 매운 맛을 내는 성분인 capsaicin은 신경독성물질로 알려져 있으며 통증을 억제시키는 효과가 있는 것으 로 밝혀져 있다. 최근까지도 capsaicin을 성장한 동물에 투여시 말초신경에만 신경독성 효과를 나타낸다고 생각하였다. 성장한 동물에 capsaicin을 투여 시에 중추신경계통에 영향을 미칠 가능성에 대하여는 일부 논문에서만이 언급하고 있다. 중추신경계통에서의 capsaicin의 영향을 조사하기 위해 일차 배양된 여러 중추신경원에 capsaicin을 처리하여 세포사멸과정을 형태학적으로 관찰하였고, capsaicin에 의해 유도되는 세포사멸 기전을 고찰하였다. Capsaicin을 처리한 후 대뇌피질 및 해마신경원은 형태학적으로 심한 변화를 보이며 죽었으며 LDH 활성도 조사에서도 대뇌피질인 경우 10 μM의 capsaicin의 농도로 24시간 처리한 결과 총 세포의 36.6±2.3% 그리고 해마 신경원인 경우는 50.1±5.0%가 세포사멸을 일으켰다. 이에 비해 신선조, 시상, 중간뇌의 신경원에서는 세포사멸이 적게 일어났다. Hoechst-PI 염색을 시행한 결과에서는 capsaicin을 처리하여 영향을 받는 신경원에서 핵의 분절 현상을 관찰할 수 있었다. 이와 같은 결과는 capsaicin에 의해 유도된 세포사멸 형태가 세포괴사 (necrosis)보다는 아폽토시스 (apoptosis) 과정을 거친다는 것을 시사해 주고 있다. 중추신경원의 세포사멸이 부위별로 다른 양상으로 일어나는 것이 이 수용체의 발현과 관련이 있으리라 예상하고 수용체의 특이하게 작용하는 항체를 이용하여 준비된 각 부위별 일차 배양 신경원에 면역화학염색을 시행하여 분포를 조사하였다. 그 결과 capsaicin 수용체는 대뇌피질과 해마 신경원에서 뚜렷하게 염색되고 나머지 신경원에서는 적게 분포함을 알았다. 이것은 세포사멸과 일치하는 내용이다. 또한 in vivo에서의 면역화학반응도 마찬가지로 대뇌피질과 해마 부위에서만 강한 양성반응을 보임으로서 in vitro 실험 결과와 일치하였다. Capsaicin은 중추신경원의 세포사멸을 유도하며, 뇌의 각 부위에 따라 그 감수성이 다름을 알 수 있었다.

상부 위장관 질환에서 IL-1β 및 IL-1RN 유전자 다형성

이준홍 ( Joon Hong Lee ),김광하 ( Gwang Ha Kim ),허정 ( Jeong Heo ),박도윤 ( Do Youn Park ),전은숙 ( Eun Sook Jun ),강대환 ( Dae Hwan Kang ),송근암 ( Geum Am Song ),조몽 ( Mong Cho ),양웅석 ( Ung Suk Yang ) 대한내과학회 2003 대한내과학회지 Vol.65 No.1

목적 : H. pylori 감염은 위,십이지장궤야, 위암 등 여러 종류의 상부 위장관 질환과 연관이 있다고 알려져 있으나, 일부의 감염자들에서만 병변을 일으킨다. 일한 H. pylori의 다양하고 예측하기 어려운 병인을 주로 세균의 병독성의 차이로 설명하려는 시도가 활발히 이루어져 왔으나 아직 확실한 결과를 얻지 못하고 있는 실정이다. 최근 연구에 의하면 IL-1β및 IL-1RN 유전자 다형성은 위산 분비, 장형화생, 만성 위축성 위염과 위암의 발생과 Background : IL-1 β and IL-1 receptor antagonist (IL-1RN) genetic polymorphisms have been associated with development of gastric atrophy and increased risk of gastric carcinoma. This study aimed to determine the effects of these polymorphisms in gastroduo

Red mud를 첨가한 흙 포장재의 강도 특성

이윤규(Lee-Yun Gyu),이준홍(Lee-Jun Hong),백승철(Baek-Seung Cheol),정동영(Jung-Dong Young),홍종현(Hong-Jong Hyun) 한국지반환경공학회 2006 한국지반환경공학회 학술발표회논문집 Vol.2006 No.-

Linoleic Acid 의 광산화와 항산화제의 방어작용

백태홍,이용국,이준홍 ( Tai Hong Park,Young Kuk Lee,Jun Heung Yi ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.6

The photooxidation of linoleic acid (LA) in the presence of methylene blue as a photosesitizer and inhibitory behavior of various antioxidants in vitro were investigated. Under air supply, LA that irradiated with visible light or red light was photooxidized only by addition of methylene blue. In the presence of methylene blue, the degree of photooxidation of LA with visible light was about twice as much as that with red leght. The photooxidation of LA in ethanol solution to which BHA (butylated hydroxyanisole), BHT (butylated hydroxyto-luene) and dl-a-Toc (dl-α-tocopherol) was added to be 0.1% in final concentration, BHA was inhibited proportionally to the concentration of BHA, but in addition to dl-α-Toc (dl-α-tocopherol) was inhibited when it`s concentration was higher than 0.1%. When methylene blue was added to ethanol solution, BHA itself was little oxidized, but dl-α-Toc was at the concentration between 10^(-3)%∼10^(-1)% and BHT was highly oxidized according to the increase of concentration. In the case of antioxidants was added to chloroform-ethanol solution containing LA to be 10^(-4)% in final concentration, the photooxidation of LA was significantly inhibited by both β-carotene and dl-α-Toc but β-carotene was effective more than dl-α-Toc. Also BHA and BHT were not effective.

Linoleic Acid 의 광산화와 항산화제의 방어작용

백태홍,이용국,이준홍,Paik, Tai-Hong,Lee, Young-Kuk,Yi, Jun-Heung 생화학분자생물학회 1992 한국생화학회지 Vol.25 No.6

Linoleic acid(LS)의 광산화와 이에 대한 각종 항산화제들의 억제효과를 in vitro에서 조사하였다. 공기의 공급하에서 백색광과 적색광에 의한 LA의 광산화 반응은 광증감제인 methylene blue를 첨가했을 때만 진행되었으며, 백색광을 조사했을 때는 적색광을 조사했을 때 보다 약 2배의 산화 증가를 나타내었다. LA의 ethanol 용액에 BHA(butylated hydroxyanisole), BHT(butylated hydroxytoluene), dl-$\alpha$-Toc(dl-$\alpha$-tocopherol) 등을 최종 농도가 0.1%되도록 첨가했을 때 LA의 광산화는 BHA의 경우에는 첨가농도에 비례하여 억제되었으며, dl-$\alpha$-Toc의 경우는 첨가농도가 0.1% 이상일 때만 억제효과를 나타내었다. 한편, methylene blue를 첨가하여 광산화 시켰을 때 BHA는 산화되지 않았으나, dl-$\alpha$-Toc은 $10^{-3}%{\sim}10^{-1}%$ 사이에서 산화가 일어났으며, BHT는 첨가농도에 비례하여 산화되었다. LA의 chloroform-ethanol 용액에 $\beta$-carotene, dl-$\alpha$-Toc, BHA, BHT를 각각 최종 농도가 $10^{-4}%$되도록 첨가했을 때 LA의 광산화 억제효과는 $\beta$-carotene>dl-$\alpha$-Toc이며, BAT와 BHT는 억제효과를 나타내지 않았다. The photooxidation of linoleic acid (LA) in the presence of methylene blue as a photosesitizer and inhibitory behavior of various antioxidants in vitro were investigated. Under air supply, LA that irradiated with visible light or red light was photooxidized only by addition of methylene blue. In the presence of methylene blue, the degree of photooxidation of LA with visible light was about twice as much as that with red leght. The photooxidation of LA in ethanol solution to which BHA (butylated hydroxyanisole), BHT (butylated hydroxytoluene) and dl-$\alpha$-Toc (dl-$\alpha$-tocopherol) was added to be 0.1% in final concentration, BHA was inhibited proportionally to the concentration of BHA, but in addition to dl-$\alpha$-Toc (dl-$\alpha$-tocopherol) was inhibited when it's concentration was higher than 0.1%. When methylene blue was added to ethanol solution, BHA itself was little oxidized, but dl-$\alpha$-Toc was at the concentration between $10^{-3}%{\sim}10^{-1}%$ and BHT was highly oxidized according to the increase of concentration. In the case of antioxidants was added to chloroform-ethanol solution containing LA to be $10^{-4}%$ in final concentration, the photooxidation of LA was significantly inhibited by both $\beta$-carotene and dl-$\alpha$-Toc but $\beta$-carotene was effective more than dl-$\alpha$-Toc. Also BHA and BHT were not effective.