밤나무혹벌 신생태종(新生態種)의 에스테라제에 관한 연구(硏究)

이경로 ( Kyung Ro Lee ),이형환 ( Hyung Hoan Lee ),이종진 ( Jong Jin Lee ),신병식 ( Byung Sik Shin ) 건국대학교 기초과학연구소 1984 理學論集 Vol.9 No.-

The esterases in the whole body of one susceptible and four resistant strains, parasited in Dan tak, Cheuk pa, Eun gi and Ei cheu, of the chestnut gall wasp. Dryocosmus kuriphilus were studied by polyacrylamide gel electrophoresis. A total number of 8 esterase bands were identified in all strains of the insects, and the activities of esterases in the pupal stage were higher than those in the larval stage. Bands 2 and 7 in resistant strain and 8 in susceptible and resistant strains were not detected at the larval stage, whereas band 8 was showed higher frequency at the pupal stage. It is conspicuous that changes of esterase band of the insect according to the host plants are caused by genetic variation and will be given the basal data in relation to the ecospecies.

밤나무혹벌의 신생태종(新生態種)에 관한 연구(硏究)

이경로 ( Kyung Ro Lee ),이종진 ( Jong Jin Lee ),신병식 ( Byung Sik Shin ) 건국대학교 기초과학연구소 1985 理學論集 Vol.10 No.-

Of the two kinds of insect pests that attack chestnut trees in Korea, the chestnut gall wasp, Dryocosmus kuriphilus inhabiting a wide area, is known to do a more serious economical harm. In order to clarify the comparision of biological characteristics between one susceptible and four resistant strains, parasited in Cheuk pa, Dan tak, Ei cheu and Eun gi of the chestnut gall wasp, morphological and physiological investigation that is the structure of ovipositor and activities of lactic acid dehydrogenase, malic acid dehydrogenase, esterase and fatty acids were measured by polyacrylamide gel electrophoresis, gas chromatography and using scanning electron microscope, respectively. The ovipositor of the chestnut gall wasp, parasited in susceptible and resistant strains that is posterior region consist of three part complex and posterior site of ovipositor showing serrate form lancet with pore cannal. In addition, ventral view of lancet with of C-shape groove. It is significant of make a marked differences between the two varieties of chestnut gall wasp. A total number of 11 lactic acid dehydrogenase bands were identified in all strains of the insects, and in susceptible varieties, LDH detected in a total 9 bands but LDH of resistant varieties detected in a total of 6 bands (Cheuk pa) and 7 bands (Dan tak). Band 1 and 2 in susceptible strains detected singular at the larval and adult stage. Also, a total number of 9 malic acid dehydrogenase bands were isolated in all strains, and in suspectible varieties, MDH were isolated in a total of 8 bands (Cheuk pa) and 6 bands (Dan tak). Band 1 and 4 in susceptible and resistant strains were identified at each stage. In general, lactic acid dehydrogenese and malic acid dehydrogenase bands were detected in resistant strains more than that susceptible strains of the chestnut gall wasp. It is significant of partial differences in enzyme between the two varieties of the insects. In addition, a total number of 8 esterase bands (larval, pupal stage) and 5 esterase bands (adult) were identified in all strains of the chestnut gall wasp. The fatty acids isolated at each varieties are palmitic acid, oleic acid, linoleic acid and 3 unidentified fatty acids. In conclusion, of the two ecotypes of the chestnut gall wasp which big damaged susceptible and resistant strains, ovipositor of make a marked differences were significant of caused by genetic variation, however, physiological differences brought out the metabolic pattern that resistant was more than susceptible strains.

하천에서의 수산자원 보호를 위한 어도시설

김동섭 ( Kim Dong-sup ),이종진 ( Lee Kyung-sik ),이경식 ( Lee Jong-jin ),황종서 ( Hwang Jong-seo ),황길순 ( Hwang Gilson ) 한국농공학회 2003 한국농공학회 학술대회초록집 Vol.2003 No.-

This study was studied in Tamjin liver that is situated jangheung-gun Jeollanm-do. Fishways that was studied with the emphasis on Ice harbour type fishway analyzed efficiencies. In result, Ice harbor type fishway discovered 12species of fish among 13species in Tamjin liver. But, most other fishways have discovered a few fishes. Because most fishways established ill station and managed badly.

밤나무혹벌 新生態種의 에스테라제에 관한 硏究

李敬魯,李炯煥,李鍾鎭,申炳湜 建國大學校基礎科學硏究所 1984 理學論集 Vol.9 No.-

The esterases in the whole body of one susceptible and four resistant strains, parasited in Dan tak, Cheuk pa, Eun gi and Ei cheu, of the chestnut gall wasp. Dryocosmus kuriphilus were studied by polyacrylamide gel electrophoresis. A total number of 8 esterase bands were identified in all strains of the insects, and the activities of esterases in the pupal stage were higher than those in the larval stage. Bands 2 and 7 in resistant strain and 8 in susceptible and resistant strains were not detected at the larval stage, whereas band 8 was showed higher frequency at the pupal stage. It is conspicuous that changes of esterase band of the insect according to the host plants are caused by genetic variation and will be given the basal data in relation to the ecospecies.

밤나무혹벌의 新生態種에 관한 硏究

李敬魯,李鍾鎭,申炳湜 建國大學校基礎科學硏究所 1985 理學論集 Vol.10 No.-

저항성 품종 밤나무(축파, 단택, 이취, 은기)에 충영을 형성하고 가해하는 밤나무혹벌과 재래종 밤나무혹벌의 생리, 생태학적 특성을 비교하기 위하여 동위효소의 활성, 변태 호르몬의 주요 성분인 지방산의 변화, 생식기관을 분석ㆍ측정한 결과는 다음과 같다. 1. 젖산 수소이탈효소(LDH)는 전 변태기를 통하여 모두 11개의 밴드가 분리ㆍ확인ㆍ측정되었다. 재래종은 9개, 축파는 6개, 단택은 7개의 밴드를 나타내었고, 밴드 1,2는 재래종의 유충, 성충에서만 그 활성을 나타냈다. 전체적으로 축파, 단택에 나타난 젖산 수소이탈효소의 밴드는 재래종 밤나무혹벌에서 대부분 나타났다. 2. 말산 수소이탈효소(MDH)는 전 변태기를 통하여 9개의 밴드가 분리ㆍ확인ㆍ측정되었다. 재래종은 3개, 축파는 7개, 단택은 6개의 밴드를 나타내었고, 밴드 1은 재래종, 밴드 4는 축파, 단택을 가해하는 밤나무혹벌에서만 그 활성을 나타냈다. 전체적으로 역시 축파, 단택에 나타난 말산 수소이탈효소도 젖산 수소이탈효소와 같이 재래종 밤나무혹벌에서 대부분 나타났다. 3. 에스테라제(ES)는 재래종과 저항성 품종을 가해하는 밤나무혹벌의 유충, 용에서는 3개, 성충에서는 5개의 밴드가 분리ㆍ확인ㆍ측정되었다. 기주식물에 따라 재래종 7개, 단택과 이취에서 8개, 축파와 은기에서 5개의 밴드가 나타났다. 그리고 밴드 1, 2, 7, 8은 재래종과 저항성 밤나무혹벌에서 특이하게 나타나 뚜렷한 변이를 나타냈다. 4. 지방산(FAA)은 재래종과 축파, 단택을 가해하는 밤나무혹벌에서 팔미트산, 올레익산, 리놀레익산과 미지물 3종 등 6종류가 분석ㆍ측정되었다. 올레익산은 모든 품종에서 유충기로 부터 용기에 이르며 감소하다가 성충에서 다시 증가하였다. 미지물*, 미지물***은 저항성 품종의 성충기를 제외하고 또 재래종의 성충기에는 특이하게도 분리되지 않았다. 5. 재래종과 축파를 가해하는 밤나무혹벌의 산란관은 말단후면에 톱니모양 구조물이 있고, 3개의 판이 융합되어 산란공 형태의 2개의 구멍이 있다. 산란관 후면 중간에 C자형의 홈이 있는데 재래종은 얕고 짧은데 비하여 저항성 품종을 가해하는 것은 깊고 길어 현격한 변이를 나타냈다. 촉각은 말단에서 일곱번째 마디까지 화학 수용기로 추정되는 구멍이 있고 그 속에 유두모양의 돌기물이 있다. 수종효소의 변화양상은 특이하여 기주식물에 따른 새로운 밤나무혹벌의 출현을 뒷받침하고 있고, 또 생식기관의 현격한 차이도 유전적 변이에 의한 자매종으로 생각된다. Of the two kinds of insect pests that attack chestnut trees in Korea, the chestnut gall wasp, Dryocosmus kuriphilus inhabiting a wide area, is known to do a more serious economical harm. In order to clarify the comparison of biological characteristics between one susceptible and four resistant strains, parasited in Cheuk pa, Dan tak, Ei cheu and Eun gi of the chestnut gall wasp, morphological and physiological investigation that is the structure of ovipositor and activities of lactic acid dehydrogenase, malic acid dehydrogenase, esterase and fatty acids were measured by polyacrylamide gel electrophoresis, gas chromatography and using scanning electron microscope, respectively. The ovipositor of the chestnut gall wasp, parasited in susceptible and resistant strains that is posterior region consist of three part complex and posterior site of ovipositor showing serrate form lancet with pore cannal. In addition, ventral view of lancet with of C-shape groove. It is significant of make a marked differences between the two varieties of chestnut gall wasp. A total number of 11 lactic acid dehydrogenase bands were identified in all strains of the insects, and in susceptible varieties, LDH detected in a total 9 bands but LDH of resistant varieties detected in a total of 6 bands (Cheuk pa) and 7 bands (Dan tak). Band 1 and 2 in susceptible strains detected singular at the larval and adult stage. Also, a total number of 9 malic acid dehydrogenase bands were isolated in all strains, and in suspectible varieties, MDH were isolated in a total of 8 bands (Cheuk pa) and 6 bands (Dan tak). Band 1 and 4 in susceptible and resistant strains were identified at each stage. In general, lactic acid dehydrogenase and malic acid dehydrogenase bands were detected in resistant strains more than that susceptible strains of the chestnut gall wasp. It is significant of partial differences in enzyme between the two varieties of the insects. In addition, a total number of 8 esterase bands (larval, pupal stage) and 5 esterase bands (adult) were identified in all strains of the chestnut gall wasp. The fatty acids isolated at each varieties are palmitic acid, oleic acid, linoleic acid and 3 unidentified fatty acids. In conclusion, of the two ecotypes of the chestnut gall wasp which big damaged susceptible and resistant strains, ovipositor of make a marked differences were significant of caused by genetic variation, however, physiological differences brought out the metabolic pattern that resistant was more than susceptible strains.