http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Regulation of Two Soluble Forms of Brain Glutamate Dehydrogenase Isoproteins by Protein Kinases
이종원,최수영,조성우,Lee, Jong-Weon,Choi, Soo-Young,Cho, Sung-Woo The Korean Society for Integrative Biology 1998 Korean journal of biological sciences Vol.2 No.2
We isolated two soluble forms of glutamate dehydrogenase isoproteins, GDH I and GDH II, from bovine brain. The regulation of GDH I and GDH II by phosphorylation and dephosphorylation has been examined in various conditions. There were dose- and time- dependent activation of the GDH isoproteins when phosphorylated by cAMP-dependent protein kinase. The phosphorylated GDH had 1.1 mol of covalently bound phosphate/mol of subunit and a 2-fold increased specific activity. The phosphorylated amino acid was identified as serine. When treated with alkaline phosphatase, the activities of the phosphorylated GDH isoproteins were reduced in dose and time dependent manner and returned to those of unphosphorylated enzymes. There were no significant differences between GDH I and GDH II in their sensitivities to the action of phosphorylation and dephosphorylation demonstrating that the microenvironmental structures of the phosphorylation site in GDH isoproteins are similar to each other, These results results suggest that the inter-conversion between less active form of brain GDH isoproteins and more active form is regulated by phosphorylation through cAMP-dependent protein kineses.
이종원(Jong Weon Lee),권순일(Soonil Kwon),송오영(Oh-young Song),박상일(Sang Il Park),성유진(Yoojin Sung),지수미(Su Mi Ji),이환호(Hwan Ho Lee),백성욱(Sung Wook Baik) 한국멀티미디어학회 2010 한국멀티미디어학회 학술발표논문집 Vol.2010 No.2
본 논문은 IT 기반의 문화콘텐츠 전문가들이 직접 다양한 지역의 과학(박물관)을 견학한 체험을 기반으로 기존 과학(박물)관들의 기술 활용 및 동향을 분석한 내용을 제시한다. 또한 전문가의 문화콘텐츠 관련 연구 경험과 지식을 토대로 미래의 과학(박물)관이 과학기술대중화를 위하여 IT 기술을 어떻게 활용될 수 있을지에 대하여 논의한다.