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제주산 양식넙치 ( Paralichthys olivaceus ) 로부터 분리된 β - 용혈성 연쇄구균 ( β - Streptococcus spp. ) 의 특성
허문수(Moon Soo Heo),송춘복(Choon Bok Song),이제희(Je Hee Lee),여인규(In Kyu Yeo),전유진(You Jin Jeon),이정재(Jung Jae Lee),정상철(Sang Chul Chung),이기완(Ki Wan Lee),노섬(Sum Rho),최광식(Kwang Sik Choi),이영돈(Young Don Lee) 한국수산과학회 2001 한국수산과학회지 Vol.34 No.4
2000년도 2회에 걸쳐 제주도 일원 넙치 양식장을 대상으로 감염어에서 분리된 연쇄구균에 대한 특성시험 결과를 요약하면 다음과 같다. 감염어로부터 분리된 연쇄구균은 형태적으로 연쇄상의 gram 양성의 구균이었고, catalase와 oxidase에 음성이었으며, 용혈성 시험에서는 β-용혈성을 나타내었다. 감염어에서 각 장기별 생균수에서는 환부, 신장, 혈액 그리고 뇌순으로 연쇄구균이 검출되었다. 약제 감수성에서는 ampicillin, ciprofloxicin 등에 감수성이 있었지만 erythromycin 등에는 감수성이 없었다. 복강에 주사하여 병원성 시험을 한 결과 10일 이내에 전량 폐사하는 강한 병원성을 보였다. 감염균의 용혈성에 대한 특성은 50℃, 30분의 배양에서 용혈활성이 빠르게 증가되어 최대값에 도달되었고 또한 pH 5.5∼6.5에서 높은 활성값을 보였다. Streptococcus spp. of bacterial pathogen of fish were isolated from the cultured flounder (Paralichthys olivaceus) in fish farm of Jeju Island. Clinical signs of the infected flounder which are the most commons symptoms are as follows: erratic swimming, darkening of the body colour, unilateral or bilateral exophthalmia, corneal opacity, hemorrhages in the opercular and the bases of the fins, and the ulceration of the body surface. Biochemical characteristics of pathogenic fish Streptococcus spp. were gram positive, spherical form, catalase negative, oxidase negative and β-haemolytic, respectively. The viable cells counted from the tissue of the diseased flounder were the largest in the order of the ulcer, the kidney, the blood and the brain. The drugs used were ampicillin, ciprofloxacin, doxycycline, gentamycin, tetracycline, erythromycin, streptomycin and oxytetracycline. Streptococcus spp. were found to be sensitive to ampiallin, ciprofloxacin, doxycycline and gentamycin, but were resistant to tetracycline, erythromycin, streptomycin and oxytetracycline. The pathogenicity of Streptococcus spp. on the cultured flounder with an abdominal cavity injection was high. The haemolytic activity of the toxin against the sheep red blood cells reached the maximum after 30 min incubation at 37℃ or 50℃. The toxin showed highest activity at pH 5.5∼6.5.
넙치 Paralichthys olivaceus 초대 배양 간세포의 난황 전구물질 합성에 미치는 estradiol-$17{\beta}$와 2,4-D의 영향
여인규,최미경,이영돈,임윤규,허문수,이제희,송춘복,Yeo, In-Kyu,Choe, Mi-Kyung,Lee, Young-Don,Lim, Yoon-Kyu,Heo, Moon-Soo,Lee, Je-Hee,Song, Choon-Bok 한국어류학회 2000 韓國魚類學會誌 Vol.12 No.3
Estradiol-$17{\beta}(E_2)$와 2,4-dichlorophenoxy acetic acid (2, 4- D)가 난황 전구물질의 합성에 미치는 영향을 넙치 Paralichthys olivaceus 간세포의 초대 배양을 통하여 조사하였다. 배양간세포의 생존율은 배양온도 $27^{\circ}C$에 서 가장 높게 나타났으며, $15^{\circ}C$에서는 생존율이 급격히 감소하여 약 50%의 생존율을 나타내었다. $E_2$에 의한 VTG 의 합성은 $10^{-6}M$에서 최대치를 나타내었다. 2, 4-D $10^{-7}\sim10^{-5}M$의 첨가에 의해 VTG의 합성은 이루어지지 않았다. 그러나, 저농도인 $10^{-8}M$에서는 VTG의 합성이 증가하였다. $E_2$ 및 2, 4-D에 의해 합성된 VTG는 $10^{-6}M$ tamoxifen의 첨가에 의해 유의하게 억제되었다 (P<0.01). 본 연구 결과에서 $E_2$와 2, 4-D의 동시 첨가는 VTG의 합성을 억제하지 않았다. 이러한 결과는 2, 4-D의 작용이 $E_2$와 유사한 작용을 가지지만, VTG의 합성에 있어서 $E_2$ 수용체에의 작용 양식은 서로 다른 것으로 추정된다. Effects of Estradiol-$17\beta(E_2)$ and 2, 4-dichlorophenoxy acetic acid (2, 4-D) on vitellogenin(VTG) production were investigated in primary hepatocyte culture of olive flounder, Paralichthys olivaceus. Highest survival rate of hepatocyte were observed at $27^{\circ}C$, which markedly declined equal to 50% of those of $15^{\circ}C$. Vitellogenin production peaked at the concentration of $10^{-6}M\;E_2$. No effect was observed on VTG production at various concentrations of 2, 4-D. However, a low concentration of 2, 4-D (ie, $10^{-8}M$) only appeared increased VTG production. $E_2$ or $10^{-8}M$ 2, 4-D-primed VTG production was markedly inhibited by the addition of $10^{-6}M$ tamoxifen to the culture medium(P<0.01). Inhibition was not affected by combinational treatment with $10^{-6}M$ $E_2$ and $10^{-6}M$ 2, 4-D. These results from the current investigation suggest that 2, 4-D mimics $E_2$, but the mechanism of reaction in inducing the $E_2$ receptor are different in VTG production in oliver flounder hepatocytes.
국내 연안에서 분리한 Vibrio vulnificus의 특성
박근태,박민정,정초록,송춘복,이제희,여인규,전유진,허문수,Park Geun-Tae,Park Min Jung,Jung Cho-Rok,Song Choon-Bok,Lee Jehee,Yeo In-Kyu,Jeon You Jin,Heo Moon-Soo 한국생명과학회 2004 생명과학회지 Vol.14 No.6
2002년 5월에서 7월사이 한국 근해로부터 총 가검물 50례 중에서 V. vulnificus은 5균주가 분리되었고, API 20E kit의동정률이 $89.4\~93.7\%$로 나타났다. V. vulnificus 16S rDNA를 증폭하여 얻은 산물을 cloning하여 염기서열을 결정하고 분석한 결과 V. vulnificus로 확인되었다. 동정된 분리균주들의 cell lysates을 SDS-PAGE로 분석하였고 표준균주로 사용된 V. vulnificus ATCC 27562와 분리 균주들을 비교해 본 결과 단백밴드pattern이 많은 차이를 보였으나 외막단백질은 V. vulnificus간에는 공통의 밴드가 확인되었으나 V. parahaemolyticus 와는 차이를 보였다. Five strains of Vibrio vulnificus, which cause serious septicemia in human worldwidely, was isolated from marine environments of Korea costal area from May to July of 2002. The isolated strains were identified by API 20E kit and partial 16S rDNA sequence analysis. 16S rDNA sequence of the isolates showed $99\%$ similarity with V. vulnificus ATCC 27562. The proteins of V. vulnificus isolates were examined by analyzing patterns of the cell lysates and outer membrane proteins (OMP). The OMP separated from cell lysates showed the common protein band. Therefore OMP profiles might be useful for the identification of V. vulnificus sp.
넙치 Paralichthys olivaceus 초대 배양 간세포의 난황 전구물질 합성에 미치는 estradiol-12β와 2,4-D 의 영향
송춘복,이영돈,이제희,임윤규,최미경,여인규,허문수 한국어류학회 2000 韓國魚類學會誌 Vol.12 No.3
Effects of estradiol-17β(E₂) and 2, 4-dichlorophenoxy acetic acid(2, 4-D) on vitellogenin(VTG) production were investigated in primary hepatocyte culture of olive flounder, Paralichthys olivaceus. Highest survival rate of hepatocyte were observed at 27℃, which markedly declined equal to 50% of those of 15℃. Vitellogenin production peaked at the concentration of 10^(-6) M E₂. No effect was observed on VTG production at various concentrations of 2, 4-D. However, a low concentration of 2, 4-D (ie, 10^(-8)M) only appeared increased VTG production. E₂ or 10^-8M 2, 4-D-primed VTG production was markedly inhibited by the addition of 10^(-6)M tamoxifen to the culture medium(P<0.01). Inhibition was not affected by combinational treatment with 10^(-6)M E₂ and 10^(-6)M 2, 4-D. These results from the current investigation suggest that 2, 4-D mimics E₂, but the mechanism of reaction in inducing the E₂ receptor are different in VTG production in oliver flounder hepatocytes.
Formalin이 넙치(Paralichthys olivaceus) 난 발생 및 부화자어에 미치는 영향
박창범,나오수,이치훈,김병호,이영돈,허문수,이정재,정상철,이기완,노섬,송춘복,최광식,이제희,여인규,전유진 한국환경독성학회 2002 환경독성보건학회지 Vol.17 No.1
Effects of formalin treatment on embryogenesis and larvae growing in fertilized eggs and hatched larvae of olive flounder, Paralichthys olivaceus were investigated. Fertilized eggs and hatched larvae were exposed to aqueous solution with 100, 200, 400, 800 and 1,000 ppm of nominal formalin concentration. They were kept in sea water of 16, 18 and 22℃, respectively. Survival rate and hatchability of fertilized eggs were high in control groups more than formalin treated groups in 16, 18 and 22℃, respectively (P<0.05). A similar effect that survival rate of hatched larvae was also observed for control and treatment groups. On the other hand, fertilized eggs treated with 400ppm formalin, were all death in kept in 22℃. These results suggest that high-dose of formalin in fertilized eggs and hatched larvae in olive flounder, Paralichthys olivaceus was inhibited the normal embryogenesis for fertilized eggs and growing for hatched larvae. Also, these inhibited effects was promoted in higher temperature.