적응신호 검출을 위한 간역화된 QNAR-AGC 시스템에 관한 연구

이원길,박규호,박송배,Lee, Won-Gil,Park, Gyu-Ho,Park, Song-Bae 대한전자공학회 1985 전자공학회지 Vol.22 No.5

자동이득 제어장치에서 신호대잡음비가 큰 신호나 중첩신호(ova.lapped signal)인 경우, 장치의 출력단에서 신호 감쇄가 일어나게 된다. 이런 문제점을 해결하기 위해 guasi-noise alone reference (QNAR)알고리즘이 발표 되었다. 그러나 이 QNAR 알고리즘을 계산의 복잡성 때문에 real time processing 이 어려운 단점을 가지고 있다. 본 논문에서는 이 문제점을 해결 하기위해 새로운 방법 즉 QNAR-Bl알고리즘을 제시 하였다. 제시된 QNAR-Bl 알고리즘은 계산의 복잡성을 대폭 감소시키면서도, 시abf레이션 결과 성능은 기존의 QNAR 알고리즘 보다 향상 되었다. This paper presents a simple method for drastic reduction of computational requirement for realization of the quasi.noise alone reference (QNAR) estimation of noise power. (1 ) The new estimator docs not bring forth performance degradation, as shown by extensive computer simulations, and is amenable to hardware implementation for certain real-time signal processing problems.

자동화된 눈 ; 전장감시용 감지기

이원길,Lee, Won-Gil 한국방위산업진흥회 1986 國防과 技術 Vol.- No.84

현대전과 통신전자 시험평가의 중요성(2)

이원길,Lee, Won-Gil 한국방위산업진흥회 1997 國防과 技術 Vol.- No.225

선진국은 현대전 및 미래전에서 통신/전자장비의 생존성이 전쟁승패의 주요 요소임을 인식하고 장비 획득규정 및 관련 군사규격을 보완하며, 취약성 시험과 EMP, EMR을 비롯한 각종 전자환경시험 능력을 강화하고 있으며, 첨단무기체계 개발에 따른 전자시험평가 능력을 지속적으로 발전시키고 있다. 하지만 우리의 경우 현대전에서 그 중요성이 부각되고 있는 취약성시험과 전자환경 시험평가 능력은 매우 미흡한 수준이다.

fMRI를 이용한 성인 편마비의 항조절점 운동이 대뇌피질의 활성화에 미치는 효과

이원길,Lee Won-Kil 대한물리치료학회 2003 대한물리치료학회지 Vol.15 No.3

This study investigated activation of cerebral cortex in patients with hemiplegia that was caused by neural damage. Key-point control movement therapy of Bobath was performed for 9 weeks in 3 subjects with hemiplegia and fMRI was used to compare and analyze activated degree of cerebral cortex in these subjects. fMRI was conducted using the blood oxygen level-dependent(BOLD) technique at 3.0T MR scanner with a standard head coil. The motor activation task consisted of finger flexion-extension exercise in six cycles(one half-cycles = 8 scans = $3\;sec{\times}\;8\;=\;24\;sec$). Subjects performed this task according to visual stimulus that sign of right hand or left hand twinkled(500ms on, 500ms off). After mapping activation of cerebral motor cortex on hand motor function, below results were obtained. 1. Activation decreased in primary motor area, whereas it increased in supplementary motor area and visual association area(p<.001). 2. Activation was observed in bilateral medial frontal gyrus, middle frontal gyrus of left cerebrum, inferior frontal gyrus, inter-hemispheric, fusiform gyrus of right cerebrum, superior parietal lobule of parietal lobe and precuneus in subjedt 1, parahippocampal gyrus of limbic lobe and cingulate gyrus in subject 2, and inferior frontal gyrus of right frontal lobe, middle frontal gyrus, and inferior parietal lobule of left cerebrum in subject 3 (p<.001). 3. Activation cluster extended in declive of right cellebellum posterior lobe in subject 1, culmen of anterior lobe and declive of posterior lobe in subject 2, and dentate gyrus of anterior lobe, culmen and tuber of posterior lobe in subject 3 (p<.001). In conclusion, these data showed that Key-point control movement therapy of Bobath after stroke affect cerebral cortex activation by increasing efficiency of cortical networks. Therefore mapping of brain neural network activation is useful for plasticity and reorganization of cerebral cortex and cortico-spinal tract of motor recovery mechanisms after stroke.

현대전자 통신전자 시험평가의 중요성

이원길,Lee, Won-Gil 한국방위산업진흥회 1997 國防과 技術 Vol.- No.224

전투에서의 승리를 위해 전자장비 및 이를 탑재한 무기체계들이 현대의 전장환경에서 성능저하 없이 정상적으로 운용될 수 있도록 장비가 설계 및 제작되어야 하며, 전장환경과 유사한 조건하에서 철저한 시험평가를 거쳐 전자정비의 신뢰성과 생존성이 보장되어야 한다.

면역학적 노화 기전에 관한 연구: T 및 B 세포의 변화

김재식,이원길,서장수,송경은,이중원,이난영,Kim, Jay Sik,Lee, Won Kil,Suh, Jang Soo,Song, Kyung Eun,Lee, Joong Won,Lee, Nan Young,Weksler, Marc E. 대한면역학회 2001 Immune Network Vol.1 No.3

Background: An immunological approach for aging mechanism appears to be important. Lymphocyte subsets analysis in peripheral blood is widely performed to assess the immune status and to diagnose and monitor various diseases. Some lymphocyte subsets are known to change with age, but only few data about age-related reference ragnes for these subsets in healthy individuals have been reported. So we attempted to report reference ranges for these subsets in each age group and review changes of the results with age for the secondary studies about immune cell function as lymphocyte blast transformation and immunoglobulin gene rearrangement (VDJ) including recombination activating genes (RAG-1 and RAG-2). Methods: Lymphocyte subset analysis was performed on 302 subjects, 189 males and 113 females with age group of all decades of life. Two color direct immunofluorescene flow cytometry (FCM) was done using $Simultest^{TM}$ IMK-Lymphocyte kit (Becton Dickinson, USA), $FACScan^{TM}$ (Becton Dickinson, USA) and $FACSCalibur^{TM}$ (Becton Dickinson, USA). Lymphocyte subsets analysed were T ($CD3^+$) and B cells ($CD19^+$), helper/inducer T ($CD4^+$) and suppressor/cytotoxic T cells ($CD8^+$), helper/suppressor ($CD4^+/CD8^+$) ratio and natural killer (NK) cells ($CD3^-CD16^+/CD56^+$). The absolute numbers of each subset were calculated from total lymphocyte counts. Data collected was analysed using SAS 6.12. A P-value of < 0.05 was considered significant. Results: We reported the counts and percentages of lymphocyte and these subsets in each age group. There were no statistically significant differences between male and female subjects. The percentage of $CD4^+$ T cells, and the count of NK cells did not show the significant difference among the various age groups. The age-related changes observed in our study were as following: 1) a decrease in the percentages of T cells, B cells and $CD8^+$ T cells ; 2) a decrease in the counts of B cells and $CD8^+$ T cells ; 3) an increase in the percentage and count of NK cells ; and 4) an increase in the $CD4^+/CD8^+$ ratio. Conclusion: The characteristics of aging process appeared to be showing a marked decrease of lympocyte subsets T and B cells as well as T8 ($CD8^+$). The age-related increase of the percentage of cells bearing NK marker can be interpreted as a compensatory consequence to cope with the decrease of T cells related to the thymic involution. These changes with age appeared to be for the secondary study about immune cell function as lymphocyte blast transformation and immunoglobulin gene rearrangement.

고성 소가야 유물전시관 - 공주대학교(누마루) + 우림 C&C + 시공테크 + 건축사사무소 옛터

이호정(Lee, Ho-jung),장기호(Chang, Ki-ho),박기석(Park, Ki-seok),이원길(Lee, Won-kil) 현대건축사 2007 월간 CONCEPT Vol.- No.99

Honokiol Inhibits Nitric Oxide-Induced Apoptosis in Rabbit Articular Chondrocytes via PI-3K/AKT Pathway

Won Kil Lee(이원길),Song Ja Kim(김송자) 한국생명과학회 2010 생명과학회지 Vol.20 No.10

Honokiol은 작은 분자량을 갖는 중국의 약초인 ‘Magnolia officinalis'에서 추출한 리간드로서 중국과 일본에서 전통 의약제로 사용되어 왔다. 이전 연구에서는 honokiol이 다양한 종류의 암에서anti-angiogenic, anti-invasive, anti-proliferative activities 등의 다양한 효과가 있는 것으로 알려져 있다. 우리는 이전 연구에서 Nitric oxide donor인 sodium nitroprusside (SNP)에 의해서 토끼 무릎관절연골세포에서 세포사멸이 일어나는 것을 보고하였다. 본 연구에서는 무릎 연골세포에서 NO에 의해 유도되는 세포사멸이 honokiol 처리농도에 의존적으로 억제되는 것을 세포형태, MTT assay, Western blot analysis 그리고 FACS를 통해 확인할 수 있었다. 또한, honokiol은 SNP에 의해 유도되는 p53의 발현 및 pro-caspase-3의 활성 저해, DNA fragmentation을 억제하였다. Honokiol에 의한 세포사멸 억제는 PI-3K의 특정 저해제인 LY294002를 SNP와 honokiol과 함께 처리하였을 때 세포의 사멸이 증가하는 것을 확인할 수 있었다. 이는 honokiol이 PI-3K/AKT pathway를 통하여 NO가 유도하는 세포사멸을 억제하는 것을 의미한다. Honokiol is a small molecular weight ligand originally isolated from the Chinese medicinal herb Magnolia officinalis, a plant used in traditional Chinese and Japanese medicine [9]. In a previous study, the effects of honokiol were shown to have anti-angiogenic, anti-invasive and anti-proliferative activities in a variety of cancers [1,3,4,11,13,17,24,29,30]. We showed previously that direct production of nitric oxide (NO) by treatment of NO donor, sodium nitroprusside (SNP), led to apoptosis in rabbit articular chondrocytes [15,16]. This study confirmed that NO-induced apoptosis was suppressed by honokiol treatment in a dose-dependent manner as determined by cell phenotype, MTT assay, Western blot analysis and FACS analysis in articular chondrocytes. Treatment of honokiol inhibited SNP-induced expression of p53 as well as DNA fragmentation in articular chondrocytes, but increased expressionof pro-caspase-3. Inhibition of SNP-induced apoptosis by honokiol treatment was rescued by LY294002, the specific inhibitors of phosphoinositide 3-kinase (PI-3K) in articular chondrocytes. Our results indicate that honokiol inhibits NO-induced apoptosis via PI-3K/AKT pathway in rabbit articular chondrocytes.

Sulforaphane에 의한 p53 및 caspase-3 의존 신호전달계를 통한 인간 연골암 세포주 HTB-94에서의 세포사멸 기전 연구

Won Kil Lee(이원길),Song Ja Kim(김송자) 한국생명과학회 2011 생명과학회지 Vol.21 No.6

Sulforaphane (SFN)은 브로콜리와 다른 십자화과 채소로부터 분리해 낸 이소시아네이트 구조를 갖는 물질이다. 최근 연구에서는 다양한 암세포에서 SFN이 세포주기를 억제하여 증식저해와 세포사멸을 유도한다고 알려져 있다. 본 연구에서는 연골암 세포주 HTB 94를 이용하여 연골암세포에서의 SFN의 세포사멸 분자적 기전을 연구하였다. SFN은 처리농도 의존적으로 연골암세포의 증식억제와 세포사멸을 유도하는 것을 세포형태변화 및 핵염색, MTT 실험을 통하여 확인하였다. Apoptotic 세포는 DNA 단편전기영동 분석을 통한 분절 DNA 확인 및 유세포분석기를 이용한 subG1 구간의 세포분석을 이용하여 확인하였다. 연골암세포주 HTB-94에 SFN 처리 시 p53 단백질의 발현증가 및 pro-caspase-3의 분해를 통한 caspase-3의 활성을 증가를 Western blot analysis를 통하여 확인하였다. 본 연구결과를 종합해 볼 때, 연골암세포주 HTB-94에 SFN을 처리시 유도되어지는 세포사멸은 p53과 caspase-3 의존적인 신호조절경로를 통하여 조절되는 것을 확인하였다. 본 연구결과는 SFN의 증식억제 효과 및 연골암세포 세포사멸 유도기전을 이용하여 향후 연골암세포의 치료약제 개발에 도움이 될 것으로 기대한다. Sulforaphane (SFN) is an isothiocyanate, isolated from glucoraphanin in broccoli and other cruciferaous vegetables. Recent studies have revealed that SFN induces anti-proliferation and apoptosis by cell cycle arrest in various cancer cells. In this study, we investigated the effect of SFN induced apoptosis in chondrosarcoma HTB-94 cells. SFN caused suppression of proliferation and apoptosis in a dose-dependent manner as determined by cell phenotype, MTT assay and FACS analysis in HTB-94 cells. Treatment of SFN led to caspase-3 activation and p53 accumulation as determined by Western blot analysis. Also, SFN significantly induced DNA fragmentation and nuclear degradation though activation of caspase-3, as detected by DNA electrophoresis and immunostaining, respectively. Our results indicate that SFN-induced apoptosis was regulated by p53 and caspase-3 dependent pathways. Furthermore, SFN may act as a potent anti-proliferation agent, and as a promising candidate for molecular-targeting chemotherapy against human chondrosarcoma cells.

의료일원화와 동서의학에 관한 연구

김재식(KIM Jay-Sik),이원길(LEE Won-Kil),서장수(SUH Jang-Soo),송경은(SONG Kyung-Eun),이중원(LEE Joong- Won),김풍택(KIM Poong-Taek),이양수(LEE Yang-Soo),강병조(KANG Byung-Jo),이죽내(LEE Juk-Nae),김두희(KIM Doo-Hie),박영춘(PARK Young-Ch 大韓醫史學會 1998 醫史學 Vol.7 No.1