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Brain dopamine systems play a central role in the control of movement, hormone release, and many complex behavior. The action of dopamine at its synapse is terminated predominately by high affinity reuptake into presynaptic terminals by dopamine transporter(DAT). The dopamine transporter(DAT) is membrane protein localized to dopamine-containing nerve terminals and closely related with cocaine abuse, Parkinsonism, and schizophrenia. In present study, the recombinant plasmid pRc/CMV-DAT, constructed by subcloning of a cDNA encoding a bovine DAT into eukaryotic expression vector pRc/CMV, was stably transfected into CV-1 cells(monkey kidney cell line). The DAT activities in the cell lines selected by Geneticin(R) were determined by measuring the uptake of [3H]-dopamine. The transfected cell lines showed 30-50 fold higher activities than untransfected CV-1 cell line, and this result implies that DAT is well expressed and localized in transfected cells. The transfected cells accumulated [3H]-dopamine in a dose-dependent manner with a Km of 991.6nM. Even though high doses of norepinephrine, epinephrine, serotonin, and choline neurotransmitters inhibited the uptake of [3H]-dopamine, DAT in transfected cell line was proven to be much more specific to dopamine. The psychotropic drugs such as GBR12909, CFT, normifensine, clomipramine, desipramine, and imipramine inhibited significantly the dopamine uptake in tissue culture cells stably transfected with DAT cDNA. Radioactive in situ hybridization was done to map the cellular localization of DAT mRNA-containing cells in the adult rat central nervous system. The strong hybridization signals were detected only in the substantia nigra pars compacta and ventral tegmental area. The restricted anatomical localization of DAT mRNA-containing cells confirms the DAT as a presynaptic marker of dopamine-containing cells in the rat brain
Multi-family houses have contributed to the housing supply in the metropolitan area with being legalized in 1986. But there are some serious problems that guidelines about Multi-family houses are necessary. In this study, I will investigate the inner space of multi-family houses in other words, unit plans of them by analyzing the characteristics of them and offer this as an important reference to make the guidelines. The common understanding ('the multi -family house has got similar to the apartment.') was verified. To be analyzed properly, the quantitative tools - space syntax and method of comparing similarity between plans - were applied. About 240 multi-family houses at Gwanak-gu in Seoul, constructed from 1986 to 2007, were collected. As a result of the time-series analysis, the similarity between the two couldn't be validated and it was found out that there are typological differences between the two. Through this study, it can be achieved to make proper guidelines of multi-family houses and by applying those to old and new towns multi-family houses will be redefined as the representative type of the urban popular housing.
This study is intended to investigate the relationship between the rebound value of P type schmidt hammer and compressive strength at early age(until lday), synthesizing the studies on estimation of compressive strength by P type schmidt hammerby this time for confirming compressive strength of 5MPa at which side form can be removed. Regression formula between rebound value of P type schmidt hammer and compressive strength is shown as K= 0.00131 e<sup>2.582ln(X)</sup> and correlativity is favorable (coefficient of correlation 0.895). After analyzing the regression line as confidence coeffient(0.05) by estimate analysis, the allowance limit of y versus x is obtained. Rebound value of P type schmidt hammer, which compressive strength is more than 5MPa, shows 29, based on the lower limit of allowance limit. Therefore, if rebound value of P type schmidt hammer is more than 29, it is thought that compressive strength of concrete exceeds 5MPa under the condition of normal ready mixed concrete.
This study is intended to investigate the relationship between the rebound value of P type schmidt hammer and compressive strength at early age(until 1day), synthesizing the studies on estimation of compressive strength by P type schmidt hammerby this time for confirming compressive strength of 5MPa at which side form can be removed. Regression formula between rebound value of P type schmidt hammer and compressive strength is shown as Y=0.00131 e2.5821m(X), and correlativity is favorable (coefficient of correlation 0.895). After analyzing the regression line as confidence coeffient(0.05) by estimate analysis, the allowance limit of y versus x is obtained. Rebound value of P type schmidt hammer, which compressive strength is more than 5MPa, shows 29, based on the lower limit of allowance limit. Therefore, if rebound value of P type schmidt hammer is more than 29, it is thought that compressive strength of concrete exceeds 5MPa under the condition of normal ready mixed concrete.
본 연구에서는 7차 교육과정에서 요구하는 교실 수업 개선의 한 방법으로 웹 자료의 활용이 초등학교 수학과의 자기 주도적 학습에 어떻게 영향을 주는가를 알아보는데 그 목적이 있다. 본 연구를 통하여 웹 자료가 아동들에게 적극적인 학습태도를 갖게 해 주며, 수학 개념 형성을 용이하게 해 주며 협동학습에 도움을 주며 수준별 학습을 강화시켜 주고 문제해결력을 신장시키고 스스로 객관적 평가를 할 수 있도록 하여 자기 주도적 학습에 긍정적 영향을 주었음을 알 수 있었다. The purpose of this study is to review, through case study, how the use of web materials has influence on the improvement of self-oriented Learning in mathematics of primary school. This is one of the ways to improve classroom lectures suggested in the 7th National Standard of Curriculum. To accomplish this, the following three studies were conducted. First, a questionnaire concerning the improvement of self-oriented Learning Skill through the use of the materials on the web was designed and analyzed. Second, the activities of the class using the web materials were recorded and the study activities of the children were observed. Third, the process of writing notes about their study using web materials was analyzed though interviews after teaching and learning. Through these studies, it has been shown how web materials contribute to the improvement of self-oriented Learning Skill.
이 연구의 목적은 최근 개정 논의 중인 `지역신문발전지원 특별법`(지역신문법)의 주요 쟁점 분석이다. 첫 번째로 지역신문발전기금 지원대상 신문사를 선정하는데 상당한 영향을 미치고 있는 지역신문법의 입법 목적을 분석했다. 입법 목적 중에서 여론의 다원화, 지역사회 균형발전, 선택과 집중의 원칙을 살펴봤다. 두 번째로 한시법인 지역신문법의 상시법 전환, 세 번째로 신문관계기금의 중복, 네 번째로 우선지원대상사 지원제도의 개선 등의 쟁점을 논의했다. 연구결과에 따르면 지역신문법의 목적은 중앙 집중적인 언론에 맞설 수 있는 경쟁력 있는 지역신문 육성하는 것이다. 두 번째로 지역신문법의 상시법 전환은 강력한 선별지원제도라는 지역신문지원의 특수성이 계속 유지될 수 있을지를 고려해야 한다. 세 번째로 신문관계기금의 중복은 해결이 쉽지 않은 문제이다. 지원사업의 차별성을 확보하지 못하고 있기 때문이다. 네 번째로 우선지원대상사 제도는 지역신문법의 취지를 반영하여 계속 유지해야 할 것이다. 앞으로 지역신문법이 상시법이 되면 지원대상과 지원기준, 지원사업의 변화가 불가피하므로 이에 대한 연구도 필요할 것이다. The purpose of this study is to analyze the major issues of the `Special Act on Assistance in Development of Local Newspapers`(Local Newspaper Act), which is being discussed recently. First, I analyzed the legislative purpose of the `Local Newspaper Act`, which has a considerable influence on selecting the newspapers supported by the `Local Newspaper Development Fund.` I looked at the diversification of public opinion, the balanced development of the community, and the principles of choice and concentration among the legislative purposes. Second, there was discussion on issues such as the transition of the `Local Newspaper Act` to the Regular Law. Third, duplication of the newspaper funds, and fourth, improvement of Priority subsidy target company system. As a result of the research, the purpose of the Local Newspaper Act is to foster competitive local newspapers that can cope with centralized media. Second, it is necessary to consider whether the transition of the local newspaper law to the regular law can continue to maintain the specificity of the local newspaper support as a powerful Selective Subsidy system. Third, duplication of newspaper funds is a difficult problem to solve. This is because it does not ensure the differentiation of support projects. Fourthly, the Priority subsidy target company system should be maintained in accordance with the purpose of the Local Newspaper Act. If the local newspaper law becomes a regular law in the future, it will be necessary to study it because it is inevitable to change the support target, the support standard and the support project.
The feeder pipes of CANDU plant have been thinning due to the flow accelerated corrosion(FAC) as the operation years increased. Accurate measurement of pipe thickness is needed to estimate the thinning rate and to evaluate the remaining life. But measuring the piping thickness manually with portable equipment during power operation is impossible because of high radiation and high temperature. So KEPRI designed on-line remote thickness monitoring equipment(FACOM-F) using personal computer and ultrasonic wave. We can detect signal in bent area of the piping. The equipment was installed at Wolsong-1 NPP and validated to be used at the actual plant operation condition
This study is intended to search for how experimental factors have effect on the rebound value of P type schmidt hammer as a<br/> part of a study on quality control of concrete by P type schmidt hammer. According to the results, as the influence of pressure,<br/> the rebound value of P type schmidt hammer increases with increase of pressure below 2.5MPa, but it shows the invariable value<br/> above 2.5MPa. As the specimen decreases in thickness, the rebound value also decreases, and so thickness of specimen needs to<br/> be more than 120mm under the condition of this experiment. As the specimen grows dry, the rebound value increases, but after<br/> drying for 15 hours, it becomes constant without an increase of rebound value. As the number of strike increases, the rebound<br/> value increases, and so it is thought that restrike should not be done. The rebound value decreases by 30% at a side of specimen,<br/> but it becomes almost constant at the position over 25mm from a side. Comparing the rebound value of upper part with that of<br/> the lower part, the former shows the lower rebound value than the latter.