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이선우(Sunwoo Lee),이옥화(Yunhua Li),김준철(Joon-Chul Kim),명평근(Pyung-Keun Myung),박창식(Chang-Sik Park),우선희(Sun-Hee Woo) 대한약학회 2006 약학회지 Vol.50 No.6
Although mammalian sperms are cryopreserved for in vitro tertilization a process of cryopreservation decreases the feruility . Acrosome reaction requires depolarization-induced Ca2+ influx and Ca2+ releases from the Ca2+stores. To examine whether the cellular Ca2+ mobilization is altered by a sperm cryopreservation we compared cytosolic Ca2+ signals between fresh and cryopreserved pig sperms using confocal Ca2+imaging. The magnitudes of depolarization-induced Ca2+increases were significantly smaller in cryopreservedsperms. Exposures to 10mM caffeine or 5μM thapsigargin elicited less Ca2+increases in th cryopreserved sperms compared to fresh sperms. In addition, progesterone-triggered Ca2+rises, that are thought to enhance acrosome reaction, werecompletely abolished in the cryopreserved sperms. These results suggest that storage and (/or) release of Ca2+ from the intracellular Ca2+from the intracellular Ca2+stores in pig sperms are significantly impaired by the process of cryopreservation.