위암 수술 중 림프절 전이의 확인을 위해 시행한 수술 중 Imprint Cytology의 결과

이영준,이성현,박순태,최상경,홍순찬,정은정,주영태,정치영,하우송,Lee, Young-Joon,Lee, Sung-Hyun,Park, Soon-Tae,Choi, Sang-Gyeong,Hong, Soon-Chan,Jung, Eun-Jung,Joo, Young-Tae,Jeong, Chi-Young,Ha, Woo-Song 대한위암학회 2005 대한위암학회지 Vol.5 No.3

목적: 위암 수술 시 주변 림프절 전이 여부의 파악을 수술 중에 하는 것은 특히 제한적인 수술을 시행하는 경우 도움이 된다. 현재 사용 가능한 방법은 동결절편법, imprint cytology, 그리고 기타 분자학적인 방법들이 있으며 최근의 대부분의 연구들에서는 동결절편 법을 이용하여 그 결과를 보고하고 있다. 본 연구에서는 저자들은 imprint cytology 방법이 위암 수술 증 림프절 전이 여부를 판단하는 데 있어서 가지는 정확도와 실행 가능성에 대해 알아보았다. 대상 및 방법 2001년 4월부터 2003년 3월까지 260명의 연속된 위암 환자를 대상으로 imprint cytology를 시행하였다. 경험이 풍부한 세포 병리학자의 슬라이드 재검토가 있은 후 그 민감도, 특이도, 정확도 등을 구하였다. 결과: 수술 중 시행하는 imprint cytology에 소요되는 시간은 평균 8분이었으며, 민감도, 특이도, 그리고 정확도는 각각 52.2%, 88.8%, 73.8%였다. 결론: 만약 민감도와 특이도가 어느 정도 만족스러운 수준으로 향상된다면 imprint cytology는 수술 중 림프절 전이여부를 파악하는 데 있어서 유용한 방법이 될 수 있다. Purpose: Intraoperative assessment of lymph node status is important when performing limited surgery in gastric cancer patients. Currently available techniques are frozen section, imprint cytology, and other molecular methods, and most current studies use the frozen section method. In the present study, the authors focused on the accuracy and the feasibility of imprint cytology as a tool to assess the lymph node status intraoperatively in gastric cancer surgery. Materials and Methods: Between April 2001 and March 2003, we performed imprint cytology of the sentinel nodes of 260 consecutive patients. After review by an experienced cytopathologist, the sensitivity, the specificity and the overall accuracy were determined. Results: The time required for intraoperative imprint cytology was 8 minutes, and the sensitivity, the specificity and the overall accuracy were 52.2%, 88.8%, and 73.8%, respectively. Conclusion: Imprint cytology can be a useful technique for assessing lymph node status intraoperatively if the sensitivity and the specificity can be improved to an acceptable level.

소아 대상 혈액배양검사 현황 파악을 위한 국내 다기관 설문조사

이영준,이지영,공섬김,연규민,홍유라,오지은,Lee, Young Joon,Lee, Ji Young,Kong, Seom Kim,Yeon, Gyu Min,Hong, Yoo Rha,Oh, Chi Eun 대한소아감염학회 2018 Pediatric Infection and Vaccine Vol.25 No.1

Purpose: Blood culture is an essential diagnostic tool and requires clear indications, proper techniques, and quality control. We aimed to investigate whether blood cultures in children are appropriate for indications, are performed correctly, and receive proper quality control. Methods: We conducted an online survey targeting pediatric infectious diseases (ID) specialists working in general hospitals and neonatologists (Neo) working at hospitals operating a neonatal intensive care unit in Korea. Results: Approximately 81.1% (30/37) of pediatric ID specialists and 72.2% (52/72) of Neo responded to the survey. Some of the respondents (33.3% of ID and 59.6% of Neo) performed blood culture as a regular test irrespective of the indication. Approximately 40% of ID and 65.4% of Neo ordered only one set of blood culture in patients suspected with bacteremia. The most commonly used disinfectant for skin preparation was povidone-iodine, while the skin preparation method varied by institution. Approximately two-thirds of the institutions were monitoring the blood culture contamination rate, whereas relatively few provided staff with feedback on that rate. In addition, less than half of the institutions were providing regular staff training on blood culture (40% of ID and 28.8% of Neo). Conclusions: The indication and methods of blood culture for children varied according to institution, and few hospitals exert effort in improving the quality of blood culture. Institutions have to strive constantly toward improvement of blood culture quality and evidence-based recommendations for pediatric blood cultures should be standardized.

고수압 조건에서 쉴드터널 세그먼트 라이닝 구조설계에 관한 연구

이영준,김기림,정건웅,홍의준,김선홍,전덕찬,Lee, Young-Joon,Kim, Ki-Lim,Jeong, Keon-Woong,Hong, Eui-Joon,Kim, Seon-Hong,Jun, Duk-Chan 한국터널지하공간학회 2015 한국터널지하공간학회논문집 Vol.17 No.3

양호한 암반조건이나 균질한 점토지반 등 불투수성 지반의 경우에도 불연속면에 의하여 수압이 작용될 수 있으므로, 설계시 지반조건에 상관없이 수압조건을 고려하고 있다. 그러나, 세그먼트 라이닝의 형상이 원형임에 따라 수압을 고려하는 경우가 오히려 라이닝에 발생되는 부재력을 감소시킬 수가 있으므로, 고수압 조건에서는 이에 대한 설계개념정립이 중요하다. 따라서, 본 논문에서는 고수압의 불투수성 지반조건에서 하중조건 및 하중조합에 따른 부재력 크기 등을 비교함으로서, 세그먼트 라이닝 설계시 합리적이고 적절한 설계개념에 대하여 서술하였다. In impermeable ground, water pressure is applied due to discontinuity such as joint or fissure. Therefore, water pressure should be considered in design regardless of ground condition. However, when the shape of segmental lining is circular, water pressure may reduce the lining member force, so it is important to define the assumption and the concept of design in case of high water pressure. This paper presents the concepts of design of the lining of shield tunnel at high water pressure and in impermeable ground condition. In addition, the member forces in various load conditions were compared in this study. (elastic equation, closed form solutions, beam-spring model).

속단의 dichloromethane 분획물이 태아골모세포의 골형성 유도에 미치는 효과

이영준,최희인,김윤철,신형식,유형근,Lee, Young-Joon,Choi, Hee-In,Kim, Yun-Chul,Shin, Hyung-Shik,You, Hyung-Keun 대한치주과학회 2003 Journal of Periodontal & Implant Science Vol.33 No.2

The ideal goal of periodontal therapy is the regeneration of periodontal tissue repair of function. Although is very difficult to attain the goal, recent advances in periodontal wound healing concepts encourage hope reaching it. Recently many efforts are concentrated on the regeneration potential of material used in traditional Korean medicine. Phlomidis Radix has been used for the treatment of blood stasis, bone fracture and osteoporosis in traditional Korean medicine. The purpose of this study is to examine effects of dichloromethane fraction Phlomidis Radix on Bone Formation in Human Fetal Osteoblasts. Human fetal osteoblastic cell line(hFOB1 1.19 ;American Type Culture Collection, Manassas, VA) were used and cells were cultured containing DMEM and dichloromethane fraction Phlomidis Radix(100 ng/ml , 1 ${\mu}$/ml, 10 ${\mu}$/ml) at 34$^{\circ}C$ with 5% $CO_2$ in 100% humidity. MTT was performed to examine the viability of the cell, and alkaline phosphatase activity was analyzed to examine the mineralization. Also bone calcification nodules were evaluated. The cellular activity of hFOB1 was increased in 100 ng/ml, 1 ${\mu}$/ml , 10 ${\mu}$/ml of dichloromethane fraction of Phlomidis Radix and especially significant increation was showed in 100 ng/ml of dichloromethane fraction of Phlomidis Radix at 6days (p <0.05). ALP level of hFOB1 was significantly increased in 100 ng/ml , 1 ${\mu}$/ml, 10 ${\mu}$/ml of dichloromethane fraction of Phlomidis Radix and especially more increation was showed in 10 ${\mu}$/ml of dichloromethane fraction of Phlomidis Radix (p <0,05). Calcification nodules of hFOB1 significantly increased in 10 ${\mu]$/ml of dichloromethane fraction of Phlomidis Radix at 21 days of incubation(p<0.05). The results indicate that dicholoromethane fraction of Phlomidis Radix has excellent effects on mineralization of hFOB1.

Buplueri Radix 의 1,2,4-benzentriol에 의해 유발된 DNA Damage에 대한 보호효과에 대한 연구

이영준,강수진,Lee, Young-Joon,Kang, Su-Jin 대한예방한의학회 2008 대한예방한의학회지 Vol.12 No.2

Objectives : Buplueri Radix (BR), used medical plant in Korea traditional medicine, contains various compounds, including a series of triterpene saponins known as saikosaponins. We performed this study for the protective effect of BR against oxidative damage induced by 1,2,4-benzentriol(BT) in human lymphocytes. Methods : In order to investigate the protective effect of BR against carcinogens, genotoxicity induced by benzene metabolite, BT were performed using cytokinesis-block micronucleus(CBMN) assay and comet assay. Results : The frequency of micronucleus at 25, 50 and $100{\mu}M$ concentration of BT were $8{\pm}2.36$, $23{\pm}2.31$, $35{\pm}4.17$ respectively. In addition of BR with concentration of 25 and $50{\mu}g/mL$, MN frequencies were significantly decreased. According to comet assay, BT induced DNA damage in a dose-dependent manner at concentration of 10 and 50 while BT with BR treatment decreased DNA breakage. No genotoxicity was observed by BR($25{\sim}50{\mu}g/mL$) treatment alone on DNA breakage. Since BT can induce DNA damage through the generation of reactive oxygen species(ROS), we examined the level of ROS in human lymphocytes treated with BT and/or BR using DCF-DA, ROS-sensitive probe. The generation of ROS in BT-treated cells was also observed, and BR addition inhibited the level of BT-induced DNA damage. Conclusions : From above results it is suggested that BR could protect the cell and DNA from pro-oxidant effect by ROS by BT

Hypoglycemic and hypolipidemic effects of Allium victorialis leaf extract in high fat diet supplied mice

이영준,Ku, Sae-Kwang,Chung, In-Kwon,Cheon, Woo-Hyun,Lee, Young-Joon 대한예방한의학회 2011 대한예방한의학회지 Vol.15 No.1

The hypoglycemic and hypolipidemic effects of Allium victorialis (AV) leaf methanol extract were evaluated in a high fat diet (HFD) supplied mice. Changes on the serum glucose, total cholesterol, triglyceride, low density lipoprotein and high density lipoprotein were examined. The effects were compared with those of a group given 250 mg/kg of metformin. After 91 days of a continuous HFD supply, the mice were showed marked hyperglycemia and hyperlipemia. However, these hyperglycemia and hyperlipemia induced by the HFD were inhibited by the AV extract treatment at the three different doses (62.5, 125 and 250 mg/kg). The results suggest that the AV methanol extract is beneficial for improving the diet-induced hyperlipidemia and hyperglycemia in humans.

Effect of Gongjindon a Traditional Korean Polyherbal Formula, on the Pharmacokinetics Profiles of Donepezil in Male SDRats (1) -Single Oral Combination Treatment of Donepezil 10mg/kg with Gongjindan 100mg/kg within 5 min-

이영준,구세광,Chung, Dae-Kyoo,Kwon, Oh-Dae,Park, Soo-Jin,Lee, Young-Joon,Ku, Sae-Kwang 대한예방한의학회 2013 대한예방한의학회지 Vol.17 No.1

Objectives : This study was aim to evaluate effects of pharmacodynamics and toxicity in combination therapy of donepezil with Gongjindan. Methods : After 10mg/kg of donepezil treatment, Gongjindan 100mg/kg was administered within 5 min. The plasma were collected at 30min before administration, 30min, 1, 2, 3, 4, 6, 8 and 24hrs after end of Gongjindan treatment, and plasma concentrations of donepezil were analyzed using LC-MS/MS methods. PK parameters of donepezil were analysis as compared with donepezil single administered rats. Results : Gongjindan markedly inhibited the absorption of donepezil regardless of sample time, from 30min to 8hrs after end of co-administration comparing with donepezil single treated rats. Especially the absorption of donepezil was significantly decreased at 2hrs after co-administration as compared with donepezil single treated rats, in the present study. Accordingly, the Cmax(-27.76%), $AUC_{0-t}$(-27.22%) and $AUC_{0-inf}$(-26.54%) of donepezil in co-administered rats were significantly decreased as compared with donepezil single treated rats, respectively. Conclusions : Based on the results of the present study, co-administration of Gongjindan decreases the oral bioavailability of donepezil by inhibiting the absorption. It is considered that the more detail pharmacokinetic studies should betested to conclude the effects of Gongjindan on the pharmacokinetics of donepezil, when they were co-administered, like the effects after co-administration with reasonable intervals considering the Tmax of donepezil and after repeated co-administrations.

혈관성 치매 치료 약물에 대한 문헌 고찰 연구 : 외국문헌을 중심으로

이영준,한창현,전원경,백경민,전우현,최성훈,Lee, Young-Joon,Han, Chang-Hyun,Jeon, Won-Kyung,Baek, Kyung-Min,Cheon, Woo-Hyun,Choi, Seong-Hun 대한예방한의학회 2011 대한예방한의학회지 Vol.15 No.2

The average human life span has increased due to the development of modern medicine and science, resulting in prolonged life expectancy and increase in the population counts of the geriatric age group. In particular, a dramatic increase of elderly patients suffering from senile disorders including neurodegenerative diseases, Alzheimer's disease(AD), and vascular dementia has become a serious social problem in public health. Thus, this study is aimed to summarize available clinical trial data on several commonly used medicines include donepezil, rivastigmine, galantamine, memantine and oriental medicine, and examine the effect of oriental medicine combined with western medicine in the treatment of patients with senile disorders using the data from literature reviews and survey studies.

세그먼트 라이닝 이음부의 모멘트-회전 관계와 지압강도 계산

이영준,정지승,Lee, Young Joon,Chung, Jee Seung 한국터널지하공간학회 2021 한국터널지하공간학회논문집 Vol.23 No.2

일반적으로 세그먼트 라이닝 터널은 프리캐스트 콘크리트 세그먼트를 연결하여 하나의 링을 구성하고 터널의 진행방향으로 링을 서로 결합하여 형성한 터널을 말한다. 세그먼트 라이닝의 구조적 특성은 세그먼트 이음부의 거동에 따라 크게 달라지므로 이음부를 적절하게 모델링해야 한다. 지반 하중을 받을 때 세그먼트 이음부는 회전에 저항하는 힌지로 작동하며, 모멘트-회전 관계는 비선형 거동을 보인다. 세그먼트 이음부가 라이닝 거동에 미치는 영향을 파악하기 위해 실제 설계에 통용되는 일본 기준 및 Janssen 모델을 적용하여 세그먼트 이음부의 모멘트-회전 관계를 설정하였다. 또한 이 논문은 지압강도를 기초로 세그먼트 이음부의 회전강성을 결정하는 방법을 제시하였다. 가상의 설계조건에서 기존 모델 및 제시된 방법을 적용해 세그먼트 이음부의 회전을 추정하고 세그먼트 라이닝과 이음부에서 발생하는 단면력을 계산하였다. 세그먼트 이음부의 회전이 증가할수록 이음부의 접촉 면적이 감소하므로 세그먼트 이음부의 지압강도를 확인해야 한다. 이 논문은 세그먼트 이음부의 지압강도를 검토하기 위해 세그먼트 이음부의 회전강성을 결정하고 지압강도를 계산하는 일관된 방법을 제시하였다. In general, segment lining tunnel refers to a tunnel formed by connecting precast concrete segments as a ring and connecting such rings to each other in the longitudinal direction of the tunnel. As the structural properties of the segment lining is highly dependent on the behavior of the segment joints, thus correct modelling of joint behavior is crucial to understand and design the segment tunnel lining. When the tunnel is subjected to ground loads, the segment joint behaves like a hinge that resists rotation, and when the induced moment exceeds a certain limit of the rotation then it may enter into non-linear field. In understanding the effect of the segment joint on the lining behavior, a moment-rotation relationship of the segment joint was explored based on the Japanese practice and Janssen's approach commonly used in the actual design. This study also presents a method to determine the rotational stiffness of joint refer to the bearing strength. The rotation of the segment joint was estimated in virtual design conditions based on the existing models and the proposed method. And the sectional force of the segment lining and joint were calculated along with the estimated rotation. As the rotation at the segment joint increases, the joint contact area decreases, so the designer have to verify the segment joint for bearing strength as well. This paper suggests a consistent method to determine the rotational stiffness and bearing strength of joints.

Effects of Hydraulic Retention Time on Hydrogen Production

이영준,Lee, Young-Joon Korean Society of Environmental Health 2005 한국환경보건학회지 Vol.31 No.4

연속반응조에서의 수소생산에 대한 수리학적체류시간(희석율, D)의 영향을 $1\%$ sucrose를 함유한 $37{\pm}1^{\circ}C$ 조건에서 조사하였다. 실험결과 수리학적체류시간(희석율)의 각각의 조건에 따라 생성된 가스중 수소성분은 $50~71\%$의 범위로 발생되었다. $H_2/CO_2$ 비율은 희석율이 증가할 때 $H_2/CO_2$ 비율도 증가하였다. 최대수소생성 수율은 희석율 0.14 l/h까지는 증가하다가 이후에는 감소하였고, 수소생성 수율은 0.81 l/g sucrose이었다. Acetate 생성 수율은 butyrate생성 수율 보다 희석율 조건변화에 민감하게 변화하였다. propionate 및 solvents는 희석율 변화에 영향을 받지 않았다 biomass 수율은 희석율이 0.2 l/h 까지는 증가하였으나, 그 이상의 조건에서는 감소하였다.