Bacillus thuringiensis var. alesti로부터 분리된 독소의 성상과 누에에 대한 독성

이영근 ( Yung Keun Lee ),조용섭 ( Yong Sup Cho ),임종성 ( Jong Sung Lim ) 한국잠사학회 1975 한국잠사곤충학회지 Vol.17 No.1

Bacillus thuringiensis var. alesti (H. Serotype III)로부터 분리된 단백질 독소의 물리화학적 성질 및 누에에 대한 생물학적 검정에 있어서 다음과 같은 결과를 얻었다. 1. Nutrient agar 배지를 사용하여 항온기에서 30℃에 세균을 배양했을 때 완전한 Bacterial lysis는 30일 걸렸고 액체 배지를 사용하여 발효기(Fermentation Jar)에 배양했을 때는 그 기간이 반으로 단축되었다. 2. 분리된 결정체들만으로 부터 추출된 단백질 독소와 포자-결정체의 혼합들로부터 분리된 독소와는 단백질 독소의 순수도에 있어서 차이가 없었다. 3. 단백질 결정체를 알카티(pH 9.5)에 용해시켜 Sodium Lauryl Sulphate Polyacrylamide gel을 사용한 전기영동을 행했을 때 분자량이 120,000, 87,000, 74,000의 3개의 단백질의 분리되었다. 4. 분리된 독소의 누에에 대한 생물학적 검정에 있어서 4령기 누에 체중 9당 LD50는 0.080μg이었다. In the studies of characterization of crystal prototoxin isolated from Bacillus thuringinensis var. alesti and its bioassay to the silkworm larvae, Bombyx mori L., the following results were obtained. 1. The complete lysis of bacteria grown on the nutrient agar at 30℃ in an incubator took 30 days, but the period could be reduced by a half in a devised broth media in a fermentation jar. 2. The protein toxin extracted directly from a mixture of crystals and spores without separation of crystals and spores was pure as same as the protein toxin extracted from only crysals separated. 3. By SLS polyacrylamide gel electrophoresis of prototoxin released from crystals in alkali (pH 9.5), three different proteins in molecular weights of 120,000, 87,000, 74,000 were separated. 4. In the bioassay of the toxin to the silkworm larvae, LDC50 per gram of the larvae in the 4th instar was 0.080 μg.

차아염소산나트륨용액의 양잠소독약제로서의 응용가능성에 관한 연구(1) -누에 경화병원균에 대한 생물학적검정-

임종성 ( Jong Sung Lim ),이영근 ( Yung Keun Lee ) 한국잠사학회 1977 한국잠사곤충학회지 Vol.19 No.1

차아염소산 나트륨용액의 실험실내에서의 간이시험을 통하여 양잠소독 약제로써의 적용가능성을 얻고 경화병원균에 대한 생물학적 약효검정에 대한 시험결과 아래와 같은 성적을 얻었다. 1. 차아염소산 나트륨용액 1,3,5%를 누에에 처리하여 약제가 누에표피에 접착됨으로써 받은 피해를 조사하였으나 아무런 해가 없는 것으로 나타났다. 2. 차아염소산 나트륨용액 1,3,5%를 누에에 침식하여 약제가 누에의 생리에 미치는 영향을 조사하였으나 약해가 없는 것으로 나타났다. 3. 차아염소산 나트륨용액의 황강병원균에 대한 약효는 생물학적 검정을 통하여 조사한 결과 동약제의 3%의 경우는 춘추잠기에 각각 7.3, 11.4% 발병율을 보였고 5%의 경우는 춘추잠기에 각각 6.7, 10.0%의 발병율을 보임으로써 무처리구의 평균 97%의 발병율을 보인데 비하여 좋은 효과를 보였다. In the previous short communication, the authors reported the applicability of Sodium Hyplochoride solution to silkworm rearing industry as a disinfectant after confirmed the fact that the chemical agent is capable to kill most of the pathogens of silkworm diseases. The authors, in the present study, attempted to investigate the influence of the chemical on the growth of silkworm larvae and the effect on the disinfection of silkworm larvae to inactivate the pathogen of yellow muscardine disease, Isaria farinosa and the results obtained are summarized as fellows. 1. The chemical agent did not affect the growth of silkworm larvae, when it was applied on silk-worm body with the concentration of 1,3,5 per cent of the agent during the rearing period. 2. The Sodium Hypochloride solution did not give any damage to silkworm larvae when larvae were fed up with 1,3,5 per cent of the solution. 3. In the bioassay of the solution on disinfection of larvae inoculated with yellow muscardine disease pathogen, the fact that the agent could control the disease was observed by showing much low infection rate in 3,5 per cent treatment compared to non-treatment control.

무시멘트 비구컵 재치환술

박명식(Myung-Sik Park),이영근(Yung-Keun Lee),김문규(Moon-Kyu Kim) 대한정형외과학회 1999 대한정형외과학회지 Vol.34 No.1

장대퇴부 도달법으로 시행된 비구주위 절골술을 이용한 비구 이형성증의 치료

박명식 ( Myung Sik Park ),박종혁 ( Jong Hyuk Park ),송광훈 ( Kwang Hun Song ),이영근 ( Yung Keun Lee ) 대한고관절학회 2006 Hip and Pelvis Vol.18 No.5

목적: 비구 이형성증에서 시행된 장-대퇴부 도달법(iliofemoral approach)을 이용한 비구주위 절골술의 임상적 및 방사선학적 결과를 보고하고자 한다. 대상 및 방법: 비구 이형성증으로 진단 후 비구주위 절골술을 시행하고 1년 이상 추시 관찰이 가능하였던 17명 17고관절을 대상으로 수술 전후의 임상 및 방사선적 소견을 비교 분석하였다. 환자의 평균 연령은 34.7세(14~61세)였으며 성별 분포는 남자 3예, 여자 14예였다. 추시 기간은 평균 26개월(12~48개월)이었다. 임상적 평가 방법으로 D`Aubigne and Postel이 사용되었으며, 방사선적 평가 방법으로 비구각, 외측 CE각, 전방 CE각, 대퇴골두 커버 정도의 변화를 비교 분석하였다. 결과: 임상적으로 D`Aubigine and Postel 점수는 12.7에서 15.1로 호전을 보였으며 및 방사선적 지표로 사용되었던 비구 각은 49.7˚에서 27.3˚, 외측 CE 각은 2˚에서 36.5˚, 전방 CE 각은 12˚에서 25˚, 대퇴 골두 커버는 57.3%에서 80.2%의 호전을 보였다(Wilcoxon Rank Sum test, p<0.05). 수술 후 합병증으로는 후주 골절이 2례, 신경마비 5 례중 외측 대퇴 표피신경 마비 3례였으며 이중 1례는 계속적인 지각이상을 호소하고 있으며, 일시적인 대퇴 신경마비1례와 좌골 신경마비 증상이 1례에서 발생하였다. 결론: 비구 이형성증 환자에서 장-대퇴 도달법을 이용한 비구주위 절골술은 임상 및 방사선학적 호전을 보이는 유용한 수술 방법이지만 신경마비 등의 합병증의 예방도 새로운 과제이다. Purpose: To evaluate the clinical and radiologic results of periacetabular osteotomy, for treating acetabular dysplasia through iliofemoral approach. Materials and Methods: We evaluated the results of 17 periacetabular osteotomies performed in 17 patients (12 female, 4 male). The osteotomies were performed through an iliofemoral approach in 17 hips. The mean age of the patients at the time of surgery was 34.7 years (range: 14~61 years). The average follow-up period was 26 months (range: 12~48 months). The D`Aubigine and Postel score was used for clinical evaluation. Radiological changes of acetabular head index, the CE angle (lateral and anterior), and the sharp angle were measured, respectively. Results: Clinically, the mean D`Aubigine and Postel scores improved from 12.7 preoperatively to 15.1 postoperatively. All four radiological measurements improved significantly after surgery (Wilcoxon Rank Sum test: p< 0.05). The following complications were noted: posterior column fracture in 2 hips and peripheral nerve dysfunction was noted in 5 patients. Of these nerve palsy, lateral femoral cutaneous nerve palsy in 3 patients, 1 patient complained troublesome, transient sciatic nerve palsy and femoral nerve palsy in 1 patient. There were no vascular complications or heterotopic bone formation and conversion to total hip arthroplasty. Conclusion: Periacetabular osteotomy by the iliofemoral approach is an effective surgical treatment for acetabular dysplasia in adults, but should be careful nerve injuries.

인공사료육(人工飼料育)에 있어서 핵질다각체(核質多角體) 바이러스에 대한 누에 품종별(品種別) 감수성(感受性)의 차이(差異)

임종성 ( Jong Sung Lim ),손해룡 ( Hae Ryong Sohn ),이영근 ( Yung Keun Lee ),설광열 ( Gwang Youl Seol ) 한국잠사학회 1980 한국잠사곤충학회지 Vol.22 No.2

인공사료사육에 있어서 핵다각체바이러스에 대한 품종별 저항성을 검토하기 위해서 7개의 장려품종을 공시해서 그 죽은 누에 비율을 조사한 결과는 다음과 같다. 1. 인공사료에 기른 누에에 핵다각체를 접종했을 때 바이러스의 10(7)/ml의 높은 농도를 적용할 경우에는 품종, 영기에 관계없이 대부분 거의 발병하는 높은 치사율을 나타냈으며 10(6)/ml의 경우도 같은 경향으로 높은 발병률을 보였다. 2. 바이러스의 농도가 10(5)/ml~10(3)/ml의 낮은 농도에서는 바이러스에 대한 감수성에 있어서 품종간에 차이를 보여주었다. 잠111×잠112와 한생1호×한생2호는 각 영기의 처리에 있어서 다른 품종보다 치사율이 낮음으로써 낮은 감수성을 보여주었다. 3. 어느 품종에 있어서나 일반적으로 2령 때가 3령 때보다 바이러스에 대한 감수성이 높았다. Practical application of silkworm artificial diet is very desirable to save labour in sericultural industry as the problem of labour shortage is becoming serious in Korea. However, silkworms reared on the artificial diet are more susceptible to viruses than those reared on mulberry leaves because of the lower anti-viral activity of gut juice of silkworms grown on artificial diet compared with that of silkworms grown on mulberry leaves. In this study, authors investigated the varietal difference of silkworm larvae, Bombyx mori L., reared on artificial diet which contained 20 percent of dried mulberry powder, in the susceptibility to nuclear polyhedrosis virus (NPV). The results showed that there is no difference in susceptibility to NPV among tested varieties when high concentration of NPV was admitted to silkworm larvae, but varietal difference appeared in lower concentration admitted. Among 7 hybrids tested, Hansaeng 1×Hansaeng 2 was most resistant to NPV with an LC50 of 2.7×10(6) and Jam 111×Jam 112 was also more resistant comparatively than other hybrids.

Bacillus thuringiensis 結晶性毒素의 生化學的 特性

李永根,姜錫權,金尙賢 한국잠사학회 1989 한국잠사곤충학회지 Vol.31 No.1

The parasporal crystals of Bacillus thuringinsis subspecies kurslaki, dendrolimus, finitimus, aizawai and israelensis were compared by polyacrylamide electrophoresis, amino acid composition and immunological analysis. In the subspecies of kurslaki, dendrolimus, finitimus and aizawai, the molecular weights of main subusnits of crystal solubilized by alkaline solution were 1.3x105 and 6.5x104 while those of subsp. israelensis were 4×104 and 1.4×104. The degradation of lepidopteran toxic subspecies crystals by silkworm midgut protease showed 6.0∼6.4 x104 molecular weight and the pattern of degradation of subsp. israelensis crystals was similar to that of alkaline solution treatment. In the amino acid composition, aspartic acid in subsp. israelensis and glutiamic acid In the other four subspecies were the most abundant. The immunological characteristics of the crystals revealed that the antibody produced against the alkali-solubilized crystal protein of subsp. israelensis reacted with only its antigen, but the crystal antigens from the other four lepidopteran toxic subspecies did cross·react with each other as well as with their own homologous antisera.

누에고름병 바이러스와 흰굳음병균에 대한 포르말린 薰煙消毒

李永根,李載昌,姜錫權,姜龍雲 한국잠사학회 1990 한국잠사곤충학회지 Vol.32 No.2

The disinfectivity of formalin fumigation was tested against. silkworm nuclear polyhedrosis virus and yellow muscardine pathogen, Beauveria bassiana. The inactivation of the virus was acquired when it was fumigated by adding 30g of potassium permanganate to 75ml of formalin per 3.3m2 of rearing room area and viability of the yellow muscardine pathogen was also lost with the same treatment of fumigation. It was also proved that the fumigation didn't give any damage to silkworm larvae when it was applied 2 or 3 times to grown larvae.

Bacillus thuringiensis 結晶性毒素의 分離와 形態比較

李永根,姜錫權 서울대학교농과대학농업개발연구소 1989 서울대농학연구지 Vol.14 No.2

Five subspecies of Bacillus thuringiensis were cultured in GYS medium and their morphogenesis was investigated. The proteineous crystals were also purified and compared with electron microscope. The subspecis reached the exponential growth phase in 3-5 hours and the bacterial lysis started after completion of sporulation in 13 hours. The pH of the culture medium during exponential growth phase decreased from 7.0 to 5.8 and then increased to about 8.0 druing sporulation. The pH remained constant during stationary phase and after completion of sporulation. The crystals were purified in discontinuous 60∼90% Renografin 60 density gradient ultracentrifugation. For all strains, the crystals banded between 70∼80% and spores between 80∼90%. The shpaes of crystals in tested strains were found to be bipyramidal, cuboidal or irregularly cubic and a wide variation in length exists ranging from small (0.55㎛) to large (1.76㎛). Width varied from 0.38 to 0.74㎛.

腦動脈瘤에 對한 治療와 低血壓下의 動脈瘤 手術

宋鎭彦,李永根,崔昌洛,姜俊基,宋將成,河榮秀,李春章,金達洙,成泰慶,安明洙 대한신경외과학회 1973 Journal of Korean neurosurgical society Vol.2 No.1

Authors have experienced 104 cases of the intracranial aneurysms with subarachnoid hemorrhage, who were proved to have aneurysm by cerebral angiography for past 8 years. Aneurysm surgery under controlled hypotension has shown far better result than that of surgery under hypothermia, carotid ligation or conservative treatment. Of the 104 cases, the site distribution of aneurysms and the result of various treatment are analyzed. Followings are the results. 1. Anterior communicating aneurysm was 44 per cent of the cases and it was most common site of the intracranial aneurysm. 19 per cent of aneurysms located on internal carotid artery at the posterior communicating artery, and middle cerebral artery aneurysm was 16%. The site distribution of aneurysms in Korean has shown marked difference from the reports of other countries. 2. Of 40 cases, who received conservative therpy, 15 cases (37.5%) were expired, and most of them were dead due to rebleeding from aneurysms. 3. The direct attack of aneurysms through intracranial approach was performed under moderate hypothermia till 1970, and controlled hypotension was introduced thereafter. In 49 cases of intracranial aneurysm surgery, 7 (14.2%) were expired following surgery. 4. Mortality of the carotid ligation for 15 cases of the intracranial aneurysms was 40 per cent. Cerebral ischemia or rebleeding was cause of death. 5. Intracranial aneurysm surgery under hypotension was carried out in 16 cases, and one case (6.2%) was expired. Induced hypotension and careful identification of aneurysm and neighbouring structures by magnifying operating glasses promoted operative result in aneurysm surgery.