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원자현미경 (AFM)을 이용한 환경오염물질에 노출된 HeLa 세포의 표면변화 연구
이시원(Si-won Lee),이수일(Soo il Lee),최진희(Jinhee Choi) 환경독성보건학회 2008 환경독성보건학회지 Vol.23 No.1
The toxicity of environmental pollutants was measured between a image of the surface topography in HeLa cells using atomic force microscopy for the possibility of toxic effect measurement and environmental monitoring. A image of the surface topography by AFM were estimated as toxic endpoints. The surface topography by AFM was observed a change of the cell surface in the environmental pollutants, but the standard of the measurement requires for the dose-effect degree. The overall results indicate that the possibility of measurement using AFM were confirmed a dose-effect degree related toxic effects, but it requres correlation between more various biomarker and AFM's measurements if the possibility of the toxic effect measurement was established.
감자T바이러스 검정을 위한 RT-PCR 및 Nested PCR 진단시스템 개발
이시원(Si Won Lee),신용길(Yong-Gil Shin),이진영(Jin-Young Lee),김영석(Young-Suk Kim),양미희(Mi Hee Yang),최인철(In-Cheol Choi) 충남대학교 농업과학연구소 2015 농업과학연구 Vol.42 No.2
Potato virus T (PVT) is a plant pathogen in the family Betaflexiviridae, group IV single-stranded positive sense RNA viruses. The major host of PVT is potato, and it has been reported in Ullucus tuberosus, Oxalis tuberosa and Tropaeolum tuberosum. This study aimed at developing reverse transcription (RT)-polymerase chain reaction (PCR) and nested PCR techniques for specific detection of PVT. Finally, Two RT-PCR primer sets were developed and verified. The RT-PCR products were amplified to 734 (PVT RT-PCR primer set 6) and 828 bp (PVT RT-PCR primer set 29) long to detect PVT. The nested PCR primer sets [PVT-N70/C20 (734→315 bp) and PVT-N75/C30 (828→529 bp)] were developed which are high sensitivity and verification for detection of PVT. Furthermore, a modified-positive control plasmid is use to verify contamination of laboratory in PVT detection. This study supported the diagnose PVT in potato or PVT related hosts.
소아 아토피 피부염에서 Cyclosporin의 치료효과
이시원(Si Won Lee),이용석(Yong Suk Lee),이승철(Seung Chul Lee) 대한피부과학회 2000 대한피부과학회지 Vol.38 No.4
N/A Background:Atopic dermatitis is a chronic skin disease, most common in childhood. Severe atopic dermatitis may require the regular use of topical corticosteroids or systemic corticosteroids. These treatments are prone to several adverse side effects. Many studies have reported that short-term use of cyclosporin is a quite effective prescription for severe atopic dermatitis of adults. Unfortunately, there have been very few studies on the use of cyclosporin for children. Objective:The purpose of this study was to examine the effect of cyclosporin in treating severe atopic dermatitis of children. Mehtods:Thirteen children who had severe atopic dermatitis were treated with cyclosporin(Neoral , Norvatis). The dose of cyclosporin was 3mg/kg/day. The disease activity has been monitored on a weekly basis, based on changes in 6 parameters of sign; erythema, exudation, excoriation, dryness, cracking, and lichenification. We employed a measurement system in which six different sites(head and neck, hands, elbows, feet, legs, and trunk)were scored on 4 different scales; 0(none), 1(mild), 2(moderate), and 3(severe). In addition to parameters mentioned above, itching and sleep-disturbance were also monitored. These were assessed by patients or their parents in the same manner. Results:After 4 weeks of cyclosporin treatment, the significant reductions in mean severity scores and mean sysmptom scores were observed. The mean severity scores of 13 patients decreased from 42.0±13.3 to 14.0±13.1(p<0.01). The mean symptom scores of itching and sleep-disturbance decreased from 2.2±1.0, 2.1±1.0 to 0.8±1.0, 0.8±1.0(p<0.01), respectively. There were 6 events of adverse side effects throughout the study. Upper respiratory tract infection was the most frequent complication from 3 patients. Conclusion:In view of these findings, we conclude that cyclosporin is effective in the treatment of severe atopic dermatitis of children without serious complications. (Korean J Dermatol 2000;38(4):466~471)