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충청북도에서 분리한 느타리버섯 갈변병 병원세균의 병원성
이혁인,이승돈,박경선,김영기,차재순 충북대학교 농업과학기술연구소 1997 農業科學硏究 Vol.14 No.-
One hundred sixty seven bacterial isolates were obtained from the bacterial brown blotch-disea-sed oyster mushrooms collected from 37 mushroom cultivation houses throughout Chungcheungbukdo. The 21(11.9 %) of them formed white line when they were cultured with white line reacting organism(WLRO) and 14 (6.5%) were classified WLRO by white line test. All white line forming isolates against WLRO skewed strong pathogenicity and hemolytic activity to rat red blood cells. Six of the white line forming isolates were identified as Pseudomonas tolaasii which is known a pa-thogen of brown blotch disease of button mushroom (Agaricus bisporus) and oyster mushroom (Pleurotus ostreatus) and four of them were Psudomonas fluorescens type G (biovar V) by BiologR bacterial identification system. WLROs consisted of heterogeneous group of bacteria ; Seven of them were identified as P. fluorescens type A (blovar I) and the rest of them were P. fluorescens type G,P. corrugata, P. tolaasii, and unidentified isolate. Some of the WLROs showed weak pathoge-nicity on rapid pitting test. P. tolaasii A- R and 6041R were mutagenized with transposon No. 5 (Tn5) and the mutants which could not form white line against WLRO were selected. All white line negative Tn5 mutants lost the pathogenicity and ability of hemolysis. The results suggest that tolaasin which is a lipodepsipeptide toxin produced by P. tolaasii and known an causal agent of white line forming in the bacterium is the primary disease determinant of P. tolaasii.