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Lipopolysaccharide 유도된 Raw264.7 세포주에서 전사조절인자 NF-κB와 IRF-1의 공동작용에 의해 조절되는 PD-1 발현연구
최은경,이수운,이수웅,Choi, Eun-Kyoung,Lee, Soo-Woon,Lee, Soo-Woong 한국미생물학회 2013 미생물학회지 Vol.49 No.4
Programmed Death-1 (PD-1) is one of the important immune-inhibitory molecules which was expressed in T cells, B cells, NKT cells, and macrophages activated by various immune activating factors. Lipopolysaccharide (LPS), the major component of the outer membrane of Gram-negative bacteria, is one of the crucial immunogens for PD-1 expression. However, there are only a few reports on the expression mechanisms of PD-1 in innate immune cells. In this study, we investigate the expression mechanisms of PD-1 in LPS-stimulated Raw264.7 cell lines by RT-PCR, Western Blot, flow cytometry as well as ChIP assay and co-immunoprecipitation. When Raw264.7 cells were stimulated with LPS, PD-1 expression was greatly up-regulated via PI3K and p38 signaling. Primary macrophages isolated from LPS-injected mice were also shown the increased expression of PD-1. In promoter assay, NF-${\kappa}B$ and IRF-1 binding regions in mouse PD-1 promoter are important for PD-1 expression. We also found that the co-activation of NF-${\kappa}B$ and IRF-1 is indispensable for the maximum PD-1 expression. These results indicate that the modulation of PD-1 expressed in innate immune cells could be a crucial for the disease therapy such as LPS-induced mouse sepsis model. Programmed Death-1 (PD-1)은 중요한 면역조절분자들 중 하나로 다양한 면역활성인자에 자극된 T 세포, B 세포, NKT 세포 및 대식세포에서 발현된다. Lipopolysaccaride (LPS)는 그람음성세균의 세포벽구성물질로 PD-1 발현을 유도하는 중요 면역원들 중 하나로 알려져 있다. 그러나 선천면역세포에서 PD-1 발현기전에 관한 연구는 미비한 실정이다. 본 연구에서는 LPS에 의해 자극된 Raw264.7 세포주를 대상으로 PD-1 발현 및 발현조전기전을 RT-PCR, Western Blot, 유세포분석기, ChIP assay 및 co-immunoprecipitation 방법으로 조사하였다. Raw264.7 세포주가 LPS로 자극되었을 때 PI3K 및 p38 신호전달경로를 경유하여 PD-1 발현이 크게 증가되었다. 또한 LPS 주사된 생쥐의 비장유래 대식세포에서도 PD-1 발현이 증가됨을 확인 하였다. PD-1 유전자의 프로모터 분석을 통해서 NF-${\kappa}B$ 및 IRF-1 결합부위가 PD-1 발현에 중요함을 알 수 있었다. 또한 PD-1 발현을 극대화하기 위하여 전사조절인자 NF-${\kappa}B$ 및 IRF-1의 공동활성이 필수적임을 확인하였다. 본 연구결과는 LPS 유도 생쥐패혈증모델에서 선천면역세포에 발현된 PD-1분자의 제어를 통한 질병 연구에 유용한 자료로 이용될 수 있을 것으로 사료된다.
중량의 차이에 따른 저항성운동이 성장기 청소년의 골대사지표에 미치는 영향
신원태(Won Tea Shin),이수운(Soo Woon Lee),이선영(Sun Young Lee) 한국발육발달학회 2013 한국발육발달학회지 Vol.21 No.4
This study was targeted adolescents in the period of growth that promotes bone growth and determines maximum bone mineral density, in order to achieve the following research purposes; first, ``Does resistance exercise positively affect bone turnover makers?``, and second, ``If it has a positive effect on them, does it depend on weight?``. For the study, 27 students in a male middle school were chosen as research subjects, and they were asked to repeat three-intensity resistance exercise programs. 9 students each were classified into three groups for offsetting the effect of the sequence and then, each group measured the effect of exercise, repeating three-intensity exercise programs in a different sequence. The three-intensity resistance exercise programs in this study were 8RM maximum exercise, 21RM maximum exercise and 16-times repetitive exercise with an intensity of 1/2 of 8RM. For measurement, blood samples were collected from the subjects before and after the exercise programs, and bone formation makers, bone resorption makers and growth hormone were analyzed as blood variables. The measurement result showed that as a bone formation maker, BALP significantly increased during all the three exercise programs, and Osteocalcin didn`t show any change. As bone resorption makers, NTx and deoxypyridinoline significantly reduced during all the three exercise programs. Growth hormone also significantly increased during all the three exercise programs. Significant changes were found in all the variables during each period, except Osteocalcin, and there was no interaction between intensity and measurement period. In conclusion, these findings imply that resistance exercise with an intensity of 8~21RM had a positive influence on bone metabolism of male middle school students, regardless of weight.
서고은,정지아,송준호,정태영,이수운,박상준,Seo, Go-Eun,Jeong, Ji-A,Song, Jun-Ho,Jeong, Tae-Yeong,Lee, Soo-Woon,Park, Sang-Jun 대한악안면성형재건외과학회 2010 Maxillofacial Plastic Reconstructive Surgery Vol.32 No.1
Oral metastatic tumors are uncommon and account for about 1% of malignant oral neoplasm. The metastatic lesions are common in breast, lung, kidney and thyroid cancer. About 20% of oral mucosal metastasis is founded before detection of primary lesions. Usually, the metastatic lesions of the oral cavity are involved in the jaw bone, especially mandible more than the oral soft tissues. We report a case diagnosed as the stomach cancer with mandible metastatic lesion looking like TMJ abscess.
당뇨유도 백서 경골에 티타늄 임플란트 매식 시 저출력 초음파 적용이 골치유에 미치는 영향
서영교,김욱규,박상준,이수운,김용덕,황대석,Seo, Young-Kyo,Kim, Uk-Kyu,Park, Sang-Jun,Lee, Soo-Woon,Kim, Yong-Deok,Hwang, Dae-Seok 대한악안면성형재건외과학회 2012 Maxillofacial Plastic Reconstructive Surgery Vol.34 No.3
Purpose: Diabetes mellitus (DM) has been shown to alter the properties of the bone and impair bone healing around a titanium implant. The aim of this study is to investigate whether the low-intensity pulsed ultrasound (LIPUS), which has been known to stimulate the bone healing, improve the osseointegration of the titanium implant in tibia of DM-induced rats. Methods: 16 rats were received streptozotocin (60 mg/kg) for inducing diabetes. A total number of 32 titanium implants were placed bilaterally into both tibiae of these rats. The right tibia of each rat received LIPUS application (10 min/day) during 7 days post-operation, while the left side received no treatment. The study was carried on for six weeks and the rats were sacrificed at 1, 2, 4, and 6 weaks postoperatively (4 rats for each week) for histomorphometric and histologic analysis. Bone-implant contact and bone area were measured. Comparisons between the groups were made using statistical analysis on histomorphometric analysis. Results: The histomorphometry parameters showed that the bone-implant contact and the bone area values have decreased in the late osseointegration periods (4, 6 weeks) compared to the early osseointegration periods (1, 2 weeks) in both two groups. The bone-implant contact values of the LIPUS group were somewhat higher than those of controls at 1, 2 weeks, but the difference was not statistically significant. The bone area values of the LIPUS group were also higher than those of controls at 1, 2 weeks, but the difference was not statistically significant as well. Conclusion: Results of this study indicate that LIPUS may have positive effects on early osseointegration but could not improve the long term stability of dental implants.
안면골 골절의 부적절한 정복에 의해 야기된 부정 교합의 재치료
윤형기,박진배,이해경,이수운,김우형,Yoon, Hyeong-Ki,Park, Jin-Bae,Lee, Hae-Kyoung,Lee, Soo-Woon,Kim, Woo-Hyeong 대한악안면성형재건외과학회 2005 Maxillofacial Plastic Reconstructive Surgery Vol.27 No.2
Malocclusion can be often found after improper reduction of facial bone fractures, especially by the plastic surgeon or other medical doctor. This causes lots of problem in esthetics, mastication, or facial symmetry. We present four cases which are related above problems. These were well treated by orthognathic surgery or orthodontic treatment.
Bisphosphonate를 단기간 투여했을 때 초기 골막 및 상악동 점막치유에 미치는 영향
임광수,서고은,송준호,이수운,박상준,Lim, Kwang-Soo,Seo, Go-Eun,Song, Jun-Ho,Lee, Soo-Woon,Park, Sang-Jun 대한악안면성형재건외과학회 2010 Maxillofacial Plastic Reconstructive Surgery Vol.32 No.1
Objective: Bisphosphonate related osteonecrosis of the jaw (BRONJ) is reported in patients taken bisphosphonate for a long time, however, the mechanism of osteonecrosis in BRONJ was not clarified yet. This study was designed to investigate the effect of short administraion zoledronate on the healing pattern of periosteum and sinus membrane after iliac bone graft into maxillary sinus. Methods: In this study, 18 Newzeland rabbits were used. The animals were divided into 2 group. In the experimental group, rabbits were treated with weekly peritoneal injection (0.06 mg/kg/week) of zoledronate for three times. In the control group, rabbits were treated with saline solution injection instead of zoledronate. Periosteum and sinus membrane were harvested from one rabbit of the experimental group and one of the control group in the fourth week. The autogeneous bone was harvested from ilium and grafted into maxillary sinus. The rabbits were sacrificed at 1, 2, 4 and 8 weeks after bone graft. The healing pattern of periosteum and sinus membrane were evaluated histologically. Results: Inflammatory reaction in the periosteum was less conspicuous and healing process appeared earlier in experimental group compared with control group at 1, 2, 4 weeks. There were no differences of microscopic findings of sinus membrane between both groups at any weeks. Conclusion: Short-term use of zoledronate decreased the inflammatory reaction and enhanced healing process in the periosteum. These findings suggest the possibility that zoledronte suppress the function of macrophages.
Zoledronate가 토끼장골에서 채취한 상악동 골이식부위 치유에 미치는 영향
송준호(Jun-Ho Song),이수운(Soo-Woon Lee),박상준(Sang-Jun Park) 대한구강악안면외과학회 2009 대한구강악안면외과학회지 Vol.35 No.3
Objective : Recently, we are interested in bisphosphonate related osteonecrosis of the jaw (BRONJ). Most of patients with osteonecrosis have taken medicine bisphosphonate for a long time. But the mechanism of osteonecrosis in BRONJ was not clarified yet. The aim of this study is to evaluate the difference of bone healing effect after bone graft from ilium to maxillary sinus in rabbits between zoledronate-treated and zoledronate-not treated groups. Method : The subjects was divided into two groups. The experimental group was 9 rabbits, treated with intraperitoneal administration of zoledronate(0.06mg/kg) once per week for 3 weeks. In control group, same procedure was applied but administerd saline instead of zoledronate. After 4 weeks, surgical operation under local anesthesia (ketamine 3.0cc, xylazine 1.0cc) was done. At postoperative 1, 2, 4, 8 weeks later, each rabbits were sacrificed and removed the bone grafted area. Gross, radiologic and histopathologic exminations of bone grafted area were performed. Result : There were no conspicuous differences of radiological findings between experimental and control groups in any experimental weeks. In experimental group, new bone formation appeared earlier than control group at 1 week after operation, and maturation of bony tissue were more conspicuous at 2 and 4 weeks after operation, compared with control group. In 8 weeks after operation, similar microscopic findings were noted in both groups. Conclusion : In the bisphosphonate-treated rabbits, new bone formation in the bone grafted area appeared earlier and bony maturation was more concpicuous, even though there were no significant differences of gross and radiological findings. These findings suggest that bisphosphonate might be promotive effect in the healing process in early stage after administration.