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      • KCI등재

        국내 육성 장미 품종 꽃잎 유래 체세포배 발생 캘러스 유도

        이수영,신주영,이영아,안창호,김예진,박필만,안혜련,이가연,정현환,Lee, Su Young,Shin, Ju Young,Lee, Young Ah,Ahn, Chang Ho,Kim, Yae Jin,Park, Pil Man,An, Hye Ryun,Lee, Ka Youn,Jung, Hyun Hwan 한국자원식물학회 2022 한국자원식물학회지 Vol.35 No.5

        This study was conducted to induce somatic embryogenic callus (SEC) derived from petals in rose. The petal explants of 3 cultivars ('Ice Wing', 'Orange Eye' and 'Pink Beauty') with different flower colors were placed on three types media (MS, SH and WPM) supplemented with 11 mg/L 2,4-D, respectively, and then cultured in the dark for 47 days. Calluses were formed at explants of all three cultivars. Also, 'Ice Wing', which were cultured in the SH as the basal medium, showed the highest callus formation rate. However, somatic embryos were generated from only petal-derived callus of 'Ice Wing', which were induced on the WPM as the basal medium, transferred it to SH basal medium supplemented with 3 mg/L 2,4-D, and 300 mg/L L-proline, and cultured for 5 weeks. The SEC has been proliferated every four weeks at the subculture interval. In addition, as a results of making a comparison of expression of RhSERK3 and RhSERK4, which is used as signal for generation of somatic embryo from callus in rose, between the SEC and petal-derived callus from 'Ice Wing' by RT-qPCR, the former showed 10 times higher RhSERK3 expression and 700 times higher RhSERK4 expression than the latter.

      • 생명보험(生命保險) 계약자(契約者)에서 B형간염(型肝炎) 표면항원(表面抗原)과 표면항체(表面抗體) 양성율(陽性率) 조사(調査) 연구(硏究) : vaccine접종(接種) 이후(以後)의 변화(變化) 양상(樣相)

        이수영,이화숙,김경희,김강석,Lee, Soo-Young,Lee, Hoa-Suk,Kim, Kyeong-Hee,Kim, Kang-Seuck 한국생명보험의학회 1986 保險醫學會誌 Vol.3 No.1

        To investigate the Positive rate of anti HBs and HBs Ag positive rate of the each group of vaccinated hepatitis B type and not-vaccinated, male 4150 female 2475, from May 1, 1984 to November 30, 1985 in the Medical department of Dae han Kyoyuk ins. Co., We examined into the HBsAg and Anti HBs by using the RPHA method and PHA method and the results which were adjusted statistically were as follows. 1. HBs Ag positive rate was 9.0%(228/2531)in 1984 and 7.0%(287/4069) in 1985, the positive rate in 1985 represented 2% lower than in 1984. 2. Anti HBs positive rate was 43.3%(1096/2531) in 1984 and 42.8%(1744/4069) in 1985, there was no significant change for two years. 3. Anti HBs positive rate in the group of the not-vaccinated hepatitis B type was 39.2%(869/2215) in 1984 and 38.8%(1333/3432)in 1985. 4. Anti HBs positive rate in the group of the vaccinated hepatitis B type more than once was 64.7%(419/647)in male and 71.5%(219/306) in female that was 66.9%(638/953) in total. 5. Anti HBs positive rate in the group of the vaccinated hepatitis B type three times was 68.7%(270/393) in male and 80.0%(156/195) in female and 72.4% in total. In this conclusion; The formation of Anti HBs in female showed more or less higher than male. 6. The cases which were detected HBs Ag and anti HBs at the same time were 4 in male and 3 in female and in the group of the vaccinated hepatitis B type, the number of HBs Ag positive cases were 8 in male and 10 in female. (On condition that we didn't distinguish $5{\mu}g/ml$, hepatitis B type vaccine, from $20{\mu}g/ml$ and after vaccinating, the lapsed time was not settled.)

      • KCI등재후보

        백일해에 대한 연령별 면역혈청학적 연구

        이수영,최의윤,김주상,안중현,최정현,마상혁,박준수,김황민,강진한,Lee, Soo Young,Choi, Ui Yoon,Kim, Ju Sang,Ahn, Joong Hyun,Choi, Jung Hyun,Ma, Sang Hyuk,Park, Joon Soo,Kim, Hwang Min,Kang, Jin Han 대한소아감염학회 2012 Pediatric Infection and Vaccine Vol.19 No.2

        목적: 우리나라의 백일해에 대한 연령별 방어면역 상태를 평가하고자 면역혈청학적 연구를 시행하였다. 방법: 국내 4개 대학병원에 방문한 건강한 소아, 청소년 및 성인을 대상으로 하였다. 대상을 연령에 따라 7개 연령군으로 구분하였고 ELISA 방법을 이용하여 백일해의 항체가를 측정하였다. 연령군별 기하 평균항체가와 각 연령군별 양성 항체가를 보이는 피험자의 비율을 조사하였다. 제조사의 지침대로, 항체가가 24.0 EU/mL 이상인 경우를 양성으로 판정하였다. 결과: 전체 1,605명의 피험자가 연구에 참여하였고, 기하 평균항체가는 $56.16{\pm}50.54EU/mL$였다. 연령군별로는 11세 미만 소아 연령군($64.78{\pm}53.24EU/mL$)이 가장 높은 기하 평균항체가를 보였다(P<0.001). 양성 항체가를 보인 피험자의 비율은 전체 대상의 68.2%였고, 11세 미만 소아 연령군(76.5%)이 가장 높은 비율을 보였다(P<0.001). 결론: 소아 연령군과 비교하여, 청소년과 성인 연령군에서 백일해에 대한 낮은 기하 평균항체가를 나타냈으며 양성 항체가를 갖는 피험자의 비율이 낮다는 것을 확인하였다. Purpose: We conducted the immunoassay of pertussis according to ages, in order to evaluate protective immunity against pertussis in Korean populations. Methods: Healthy subjects were enrolled at four university hospitals in Korea. The subjects were grouped as seven age groups (every 10 years). Antibodies against pertussis toxin (PT) in sera were measured by enzyme linked immunosorbent assay (ELISA) kits. Geometric mean concentrations (GMC) of antibodies and the ratios of the subjects with seroprotective antibody levels were determined. The subjects with antibody titers ${\geq}24.0EU/mL$ were considered to seroprotective as the manufacturer's protocol. Results: Total 1,605 subjects (age: 2 months-65 years) participated in this study, and their GMC was $56.16{\pm}50.54EU/mL$. Among seven age groups, age group <11 year showed the highest GMC ($64.78{\pm}53.24EU/mL$) (P<0.001). In the analysis of the ratios of the subjects with seroprotective antibody titers, 68.2% of the subjects were proven to seroprotective, and age group <11 year also showed the highest ratio (76.5%) (P<0.001). Conclusions: We found that adolescences or adults (age group ${\geq}11$ year) showed lower levels of antibody against pertussis and lower ratio of the subjects with seroprotective antibody titers than children (age group <11 year).

      • KCI등재

        용접방법에 의한 타이타늄 연결부의 기계적 성질에 관한 연구

        이수영,장익태,허성주,임순호,Lee, Soo-Young,Chang, Ik-Tae,Heo, Seong-Joo,Yim, Soon-Ho 대한치과보철학회 1999 대한치과보철학회지 Vol.37 No.5

        The use of pure titanium and titanium alloys have been increased recently in fixed, removable prosthodontics and implant fields as a framework. But when they were used for superstructures of implant or metal framework of removable prosthesis, welding is necessary to reconnect the fracture site to control the casting distortions. To overcome the difficulties in soldering the titanium due to high oxidation property, much effort have been devoted. In this study, some of mechanical properties were compared between pure titanium and Ti-6Al-4V alloy by using after welding, electron beam welding technique and tungsten arc welding. Mechanical properties such as tensile strength, yield strength, elongation and microhardness were measured. And, in order to compare the effect of welding site and surrounding metal tissue according to the welding condition, SEM photographs were taken and element distribution was observed by Wave Dispersion Spectroscopy. Through analyses of the data, following results were obtained; 1. In items such as tensile strength, yield strength and elongation according to the welding techniques of pure titanium, only tungsten arc welded group showed significant lower value than other groups(P<0.05). 2. In items such as tensile strength and yield strength according to the welding techniques of Ti-6Al-4V alloy, control group and tungsten arc welded group showed significant difference among all the groups(P<0.05). 3. Ti-6Al-4V alloy exhibited significantly greater elongation than control group when the laser welding method and electron beam welding method were used, and elongation showed increasing tendency. 4. Pure titanium specimens exhibited increasing tendency of microhardness regardless of the weld-ing technique applied, and especially tungsten arc welded group demonstrated a great increase of microhardness than parent metal. 5. There was no hardness change in laser welded group and electron beam welded group of Ti-6Al-4V alloy, but in tungsten arc welded group, hardness changed greatly from parent metal to weld seam. 6. Through the metallographic examination and scanning electron microscopy, laser welding caused central fusion and recristallizations were formed and tungsten arc welding caused localized fusion to 0.3-0.7mm from the surface.

      • SCOPUSKCI등재

        국내 11-12세 소아에서 Td 백신 추가접종의 면역원성과 안전성 평가

        이수영,곽가영,목혜린,김종현,허재균,이경일,박준수,마상혁,김황민,강진한,Lee, Soo Young,Kwak, Ga Young,Mok, Hye Rin,Kim, Jong Hyun,Hur, Jae Kyun,Lee, Kyung Il,Park, Joon Su,Ma, Sang Hyuk,Kim, Hwang Min,Kang, Jin Han 대한소아청소년과학회 2008 Clinical and Experimental Pediatrics (CEP) Vol.51 No.11

        Purpose : This study was undertaken to evaluate the immunogenicity and reactogenicity of Td booster immunization in early preadolescents of Korea. Methods : Healthy preadolescents, who had been vaccinated with 4 or 5 doses of DTaP vaccines until 6 years old age, were enrolled in this study from August 2006 to April 2007. Diphtheria and tetanus anti-toxoid antibodies in sera were measured by ELISA just before vaccination and 4 weeks after vaccination to evaluate immunogenicity. Local and systemic adverse reactions observed for 4 weeks after vaccination to access reactogenicity. Results : 183 preadolescents were enrolled and mean age was $11.40{\pm}0.51$ years old. All subjects achieved seroprotective diphtheria and tetanus anti-toxoid antibodies (titers ${\geq}0.1IU/mL$) after Td booster vaccination. Among 183 vaccinees, 73.8% showed local adverse reactions and 37.2% systemic adverse reactions. Pain at injection site (66.1%) was the most common local reaction, and the most commonly shown systemic reaction was myalgia (17.5%). The adverse reactions were spontaneously relieved within three days after vaccination. Conclusion : Td vaccine in this study was high immunogenic and showed an acceptable tolerance in Korean preadolescents. Td booster vaccination at 11-12 years old is the most effective method to increase compliance of the vaccination and to decrease the incidence of diphtheria and tetanus. 목 적: 11-12세 연령에 Td 백신 1차 추가접종을 하는 방법에 대한 면역원성과 안전성을 평가하기 위해 연구를 계획하였다. 방 법: 2006년 8월부터 2007년 4월까지 연구병원 소아청소년과 외래에 Td 백신 접종을 받기 위해 내원한 11-12세의 소아를 대상으로 하였다. 면역원성을 평가하기 위하여 접종 전 및 접종 4주 후에 혈액을 채취하여 디프테리아 및 파상풍에 대한 항독소 항체가를 측정하였고 이상반응을 평가하기 위해 관찰 일지에 국소 및 전신 이상반응을 기록하였다. 결 과: 총 183명이 연구에 참여하였고 이들의 평균 연령은 $11.40{\pm}0.51$세이었다. Td 백신 접종 전후의 GMC는 디프테리아에 대해서는 10배, 파상풍에 대해서는 26배 이상 증가하였고, 접종 후 디프테리아와 파상풍에 대한 항체 양전율(항체가 ${\geq}0.1IU/mL$ 기준)은 100%이었다. 디프테리아의 접종 전 항체가가 0.1 IU/mL 이상인 피험자는 142명(77.6%)이었고 접종 후 항체가가 1.0 IU/mL 이상인 피험자는 174명(95.1%)이었다. 파상풍의 접종 전 항체가가 0.1 IU/mL 이상인 피험자는 146명(79.8%)이었고 접종 후 항체가가 1.0 IU/mL 이상인 피험자는 181명(98.9%)이었다. 접종 후 국소 이상반응이 73.8%, 전신 이상반응은 37.2%에서 발생하였으나 대부분 3일 이내 소실되었다. 결 론: 매우 높은 면역원성과 심하지 않은 이상반응을 고려할 때, Td 백신의 접종을 11-12세 시행하는 것은 디프테리아와 파상풍에 대한 가장 경제적인 방어 수단이며, 접종 순응도를 효율적으로 높일 수 있는 방법이다.

      • KCI등재

        전악인상채득시 인상재가 경석고 모형의 정확도에 미치는 영향

        이수영,장익태,Lee, Soo-Young,Chang, Ik-Tae 대한치과보철학회 1993 대한치과보철학회지 Vol.31 No.2

        The purpose of this study was to evaluate the reproducing accuracy of stone casts made from complete arch impressions using different impression materials. The impression materials studied were 1) polyether, 2) polysulfide, 3) polyvinyl siloxane, and 4) irreversible hydrocolloid. Impressions were made from a partially edentulous acrylic resin model with metal inserts in canine, first molar region bilaterally and mid palatal vault region. Each distance between 2 out of 5 meauring points was measured by using 3dimensional measuring machine. Impressions were poured at once with a type IV dental stone. Data were analyzed using t-test with a sample size of six. The results were as follow : 1. Polysulfide and polyether were significantly superior to polyvinyl siloxane and irreversible hydrocolloid in reproducing full arch model(p<0.05), but there were no statistical differences in amount of dimensional reproducing accuracy for full arch impression between polysulfide and polyether(p>0.05). 2. There were statistical differences in amount of dimensional reproducing accuracy between edentulous area and tooth bound area for polysulfide and polyether(p<0.05). but no statistical differences were observed for polyvinyl siloxane and irreversible hydrocolloid(p>0.05).

      • 코로나19로 본 공연예술계 충격과 그 대응 방안

        이수영,Lee, Soo-Young 한국디지털정책학회 2021 한국디지털정책학회논문지 Vol.19 No.3

        본 연구는 코로나19가 공연예술계에 가져온 국내·외의 변화를 살펴보고 그 대응방안을 도출하기 위하여 언론리뷰와 문헌조사를 통하여 내용 분석을 실시하였으며 다음과 같은 결론을 도출하였다. 첫째, 코로나19 이후 세계 모든 극단과 공연장은 공연 스트리밍에 적극적인 입장을 취한 것으로 나타났다. 둘째, 공연 영상화가 적극적으로 이루어졌으며, 이러한 공연 영상화는 기존 극장 공연의 보완재가 되고 있었다. 셋째, 공연 제작 소비 행태의 변화에서는 빈익빈 부익부의 현상이 나타났으며, 다양한 공연들을 온라인으로 경험할 수 있는 기회를 제공하는 것으로 나타났다. 넷째, 코로나19 이후 영국의 경우 직원 고용유지 지원책과 자영업자 및 프리랜서 지원책, 영국 문화예술위원회의 긴급 지원금 정책이 있으며. 한국 정부는 공연예술관람료, 예술인을 위한 창작 준비금, 학교예술강사 생계유지를 위한 강사비 등의 제도가 발표, 집행 중에 있다. 다섯째, 코로나19 이후의 대응방안으로는 공연 영상화에 꾸준히 자본과 기술을 투입하여 온라인과 오프라인이 상생할 수 있는 구조 마련이 시급하며 해외 유수 공연 단체와의 전략적 제휴와 플랫폼 공유 전략이 필요하다고 분석되었다. The purpose of this study is to establish the most severe impacts of Covid-19 in the field of the performing arts and to explore countermeasures taken both at home and abroad. This study has been conducted through literature surveys. The conclusion is as follows. First, since the outbreak of Covid-19, theater venues around the world have actively taken part in uploading recorded performances to streaming services. Secondly, these performance visualizings are generally considered as being complimentary goods rather than a substitute for live performance. Thirdly, although more audiences are tuning into watch on-line performances, consumption is concentrated on a few theaters which have a worldwide reputation and a broad range of content. Fourthly, to tackle the impact that Covid-19, the UK government announced a series of job protection schemes in the field of the Arts. In addition, Arts Council England prepared an emergency response package. In Korea, some countermeasures such as government support for artists and cultural establishments have also been implemented. Lastly, some suggestions for the sector. I conclude that there is an need for domestic companies to secure core contents of significant quality and to make strategic alliances with leading overseas performance companies so that they may cooperate together.

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