인간포배기 배아의 효과적인 유리화 동결법의 개발을 위한 연구

이상민,이주희,이상원,이승민,윤산현,임진호,박흠대,이성구,Lee, Sang-Min,Lee, Ju-Hee,Lee, Sang-Won,Lee, Seoung-Min,Yoon, San-Hyun,Lim, Jin-Ho,Park, Huem-Dai,Lee, Seong-Goo 대한생식의학회 2003 Clinical and Experimental Reproductive Medicine Vol.30 No.3

Objective: The purpose of this study was to evaluate the survival rate of vitrified blastocyst according to the freezing vessels, equilibration time in cryoprotectant and artificial dehydration method. Methods: Human blastocysts were vitrified after loading onto the plastic straw, open-pulled straw (OPS), electron microscopy grid (EM grid) for 1.5 min or 3 min. They also were directly plunged into LN2 within 30sec. For artificial shrinkage of blastocysts, 36 gauge fine needle was pushed at the cellular junction of the trophectoderm into the blstocoele cavity until it shrank without damage of inner cell mass. Results: The survival rate of vitrified blastocysts on plastic straw, OPS, EM grid as freezing vessels were 26.7, 13.0 and 60.5%, respectively. The survival rate of EM grid was significantly higher than that of plastic straw and OPS (p<0.05). For 1.5 min equilibrium, the survival rates of early blastocyst (EB), middle blastocyst (MB) and late blastocyst (LB) were 64.4, 81.0, and 20.0% respectively. For 3 min equilibrium, the survival rates of EB, MB, and LB were 69.9, 50.0 and 57.5% respectively. The survival rates of EB and MB were significantly higher than that of LB in 1.5 min equilibrium group (p<0.05), however, the significance was not observed in 3 min equilibrium groups. In cytoplasmic shrinkage before vitrification, the survival rates of EB, MB and LB were 92.9, 100 and 75.9% respectively. The survival rate of MB was significantly higher than that of LB (p<0.05). The survival rates of vitrified blastocysts by artificial dehydration and slow-frozen blastocysts were not significantly different as 88.9 and 66.7%, respectively. Conclusion: This study showed that the vitrification of human blastocysts using EM grid and artificial dehydration is an effective method. Therefore, these methods would be an useful techniques for blastocyst cryopreservation.

대식세포주에서 인슐린이 $I{\kappa}B/NF-{\kappa}B$ 경로 활성화에 미치는 영향

이상민,장연실,이춘택,김영환,한성구,심영수,유철규,Lee, Sang-Min,Jang, Yeon-Sil,Lee, Choon-Taek,Kim, Young-Whan,Han, Sung-Koo,Shim, Young-Soo,Yoo, Chul-Gyu 대한결핵및호흡기학회 2010 Tuberculosis and Respiratory Diseases Vol.68 No.3

Background: Sepsis still has a high mortality rate despite adequate supportive care. Newer therapeutic modalities have been developed but they have generally ended in failure. Recently, insulin was reported to have an anti-inflammatory effect by inhibiting the $I{\kappa}B/NF-{\kappa}B$ pathway, and may have therapeutic potential in sepsis. However, the precise mechanism of the anti-inflammatory effect of insulin is unclear. This study examined the role of insulin in activating $I{\kappa}B/NF-{\kappa}B$ in macrophage. Methods: Raw 264.7 cells, a murine macrophage cell line, were used in this experiment. Western blotting using $I{\kappa}B$ Ab and phosphor-specific $I{\kappa}B$ Ab was performed to evaluate the degradation and phosphorylation of $I{\kappa}B$ cells. For the $I{\kappa}B$ Kinase (IKK) activity, an immune complex kinase assay was performed. The level of interleukin-6 (IL-6) was measured by ELISA to determine the level of proinflammatory cytokine. Results: $I{\kappa}B{\alpha}$ degradation began 30 min after lipopolysaccharide (LPS) treatment. However, an insulin pretreatment suppressed the $I{\kappa}B{\alpha}$ degradation caused by the LPS treatment. The phosphorylation of $I{\kappa}B{\alpha}$ and IKK activity was also inhibited by the insulin pretreatment. Finally, the insulin pretreatment showed a tendency to suppress the induction of IL-6 by LPS. Conclusion: Insulin might have an anti-inflammatory effect though partial inhibition of the $I{\kappa}B/NF{\kappa}B$ pathway in macrophage cell lines.

사료의 $\alpha-cellulose$ 함량이 조피볼락 Sebastes schlegeli의 성장, 사료 효율 및 체성분에 미치는 영향

이상민,이종윤,LEE Sang-Min,LEE Jong Yun 한국양식학회 1994 韓國養殖學會誌 Vol.7 No.2

사료의 $\alpha-cellulose$ 함량이 조피볼락의 성장, 사료 효율, 영양소 이용 효율 및 체조성에 미치는 영향을 조사하기 위해 209 전후의 조피볼락 치어를 대상으로 사료의 $\alpha-cellulose$ 함량이 $0\~20\%$가 되도록 총 5종의 실험 사료를 제조하여 10주간 사육 실험한 결과는 다음과 같다. $\alpha-cellulose$ 함량 $10\%$ 첨가구까지는 대조구와 유의차없이 성장 및 사료 효율이 양호하였고, $\alpha-cellulose$ $15\%$이상 첨가 실험구들은 유의하게 낮은 값을 보였다(P<0.05). 일간 먹이 섭취량은 $\alpha-cellulose$ $15\%$ 이상 첨가구에서 유의하게 높은 결과를 보였다(P<0.05). 영양소 축적효율 중 단백질 및 에너지 축적 효율은 $\alpha-cellulose$ $10\%$ 첨가구까지 대조구와 유의차 없이 좋은 결과를 보인 반면, 지질 축적 효율은 a-cellulose 함량이 증가함에 따라 계속 감소하였다 (P< 0.05). 전어체의 단백질 함량은 실험 구간별로 차이가 없었지만, 지질 함량은$\alpha-cellulose$ $10\%$ 첨가구에서 유의하게 높은 값을 보였다(P<0.05). Experimental diets containing 5 different levels of a-cellulose $(0\%,\;5\%,\;10\%,\;15\%\;or\;20\%)$ were fed to juvenile Korean rockfish (20.5g of initial mean weight) for 10 weeks to study their ability to utilize dietary $\alpha-cellulose$. Weight gain, feed efficiency, and protein and energy retention efficiency in the fish fed diets containing $0\%,\;5\%,\;or\;10\%$ of $\alpha-cellulose$ were significantly higher than those in the fish fed diets containing $15\%\;or\;20\%$ of $\alpha-cellulose$ (P<0.05). Whole body lipid content of the fish fed $10\%$ $\alpha-cellulose$ diet were significantly higher than that of fish fed other diets (P<0.05). The results suggest that the optimum dietary fiber levels for Korean rockfish would be less than $10\%$.

Brunnstrom Stage (II)

이상민,Lee, Sang-Min 대한물리치료사협회 1986 물리치료 Vol.42 No.-

『동아시아의 아카이브 비교연구』 곽건홍 저, 도서출판 선인, 2016

이상민,Lee, Sang-Min 한국기록학회 2016 기록학연구 Vol.0 No.49

Valine 및 Alanine 분자로 표면 처리된 수용성의 ZnS 나노입자의 합성 및 특성연구

이상민,김기문,황청수,Lee, Sang-Min,Kim, Ki-moon,Hwang, Cheong-Soo 대한화학회 2009 대한화학회지 Vol.53 No.5

본 논문에서는 구조가 간단하고 생체분야에 응용 가능한 valine, alanine등의 아미노산 분자를 이용하여 수용성 ZnS 나노입자를 합성하였다. 얻어진 고체 생성물들은 XRD, HR-TEM, 및 EDXS분석으로 그 특성을 조사하였다. HR-TEM image 분석의 결과 크기가 대략 3.3에서 3.6 nm 의 입자들이 만들어 졌음을 확인 할 수 있었으며, 나노 입자 표면의 아미노산 분자들은 FT-IR 과 FT-Raman spectrum 을 얻어 결합 모드 등을 분석하였다. Water soluble ZnS nanocrystals were synthesized by capping the surface of the nanocrystals with valine and alanine molecules, which are structurally simple and bio friendly amino acids. The obtained ZnS nanocrystal powders were characterized by XRD, HR-TEM, and EDXS spectroscopies. The measured particle sizes by HR-TEM images were in the range of 3.3 to 3.6 nm. In addition, the surface capping amino acids molecules were characterized by FT-IR and FT-Raman spectroscopies.

지역특성을 고려한 학교규모 적정화 방향 설정에 관한 연구

이상민,조창희,Lee, Sang-Min,Cho, Chang-Hee 한국교육녹색환경연구원 2016 교육·녹색환경연구 Vol.15 No.1

본 연구는 적정규모학교의 효율적인 육성을 위하여, 적정규모학교에 대한 이론적 고찰, 국내 학생수 및 학교현황 분석, 적정규모학교 육성사업 현황분석, 적정규모학교 가이드라인(안) 제안 등의 순서로 연구를 진행하였다. 적정규모학교 기준(안)은 시 도별 특성과 지역의 여건을 고려하며, 기존 선행연구들의 교육적 효과와 교육과정의 운영 효율성을 고려하여 이상적인 방향으로 제안하고자 한다. 본 연구에서는 적정규모학교의 효율적인 육성을 위하여, 적정규모학교에 대한 이론적 고찰, 국내 학생수 및 학교현황 분석, 적정규모학교 육성사업 현황분석을 통해 적정규모학교 가이드라인(안)을 제안하였다. School appropriate scale refers to the size of the school to minimize students' educational deficits and ensure an appropriate level of students, the number of classes, class size, etc., and maximize educational effects. Difficult set of absolute standards appropriate scale school, contemporary social situations and educational effects, and seek a comprehensive and reasonable direction, considering this is required. School appropriate scale it is preferable to consider the effect of conditions in areas such as education, curriculum, but it is common to set the range in terms of school operational effectiveness, and efficiency of education funding, school environment. So the concept of the appropriate scale including the school's status changes associated with school size, is seeking a new direction is needed so that the school environment, etc. are considered.

휴대용 GPS를 이용한 부선의 안전예항시스템 구축에 관한 연구

이상민,안병길,Lee, Sang-Min,Ahn, Byung-Kil 해양환경안전학회 2008 海洋環境安全學會誌 Vol.14 No.4

피예선인 부선으로 인하여 예선 자체의 조종성능에 제한을 받고, 이러한 예부선은 바람, 파도, 조류 등과 같은 외력의 영향이 크게 작용하여 예부선 운항의 안정성 확보에 많은 어려움이 수반되고 있다. 예선의 선장, VTS 및 육상의 운항부서 등에서는 부선과 같은 피예선의 실해역에서의 침로, 속도, 위치 등을 실시간으로 추적하여 해난사고를 사전에 예방할 수 있는 시스템의 구축이 절실히 필요한 실정 이다. 본 연구에서는 비교적 저렴하고 이동이 용이한 휴대용 GPS를 이용한 부선의 안전예항시스템의 구축에 대한 기초적인 방법을 제안하고, 이를 토대로 실선에서의 테스트를 실시하고 그 결과에 대하여 논의한다. 본 연구를 통하여 안전 예항에 필요한 여러 항해 정보를 취득할 수 있었으며, 이러한 정보는 다양한 분야에서의 활용이 가능할 것으로 사료된다. The tug boat is restricted in her maneuvering ability due to the towed barge, and tug-barge has been strongly affected by the external forces, i.e. the wind, wave, currents, and so on. Therefore, it is difficult to get the safety of tug-barge operation. It is necessary for the captain of tug boat, VTS and operation department to develope the system which can keep observing the course, speed and position of the towed barge in actual sea and prevent the marine accidents in advance. In this study, we proposed the basic method to develope the safety towing system for barge using portable GPS which was easily movable and relatively inexpensive. Then we conducted the test on the real barge. As a result, we could obtain diverse navigation data to make a safety towing work. It was noted that these data could be used for carious purposes.

파워 분배망을 고려한 디지털 회로 시스템의 설계와 분석

이상민,문규,위재경,Lee, Sang-Min,Moon, Gyu,Wee, Jae-Kyung 대한전자공학회 2004 電子工學會論文誌-SD (Semiconductor and devices) Vol.41 No.4

This paper presents the channel analysis considering power distribution network(PDN) system of PCB. For achieve the target PDN system we proposed the useful design approach for acquiring the characteristic target of power distribution network in overall frequency ranges. The proposed method is based on the hierarchical approach related to frequency ranges and the path-based equivalent circuit model to consider the interference of the current paths between the decoupling capacitors and the board through it is a lumped model for fast and easy calculation, experimental results show that the proposed model is almost as precise as the numerical analysis. The analysis of PDN system shows that although the effective inductance of package dominatly affects the power noise and the signal transfer through data channel, the board PDNs also can not be neglected for achieving the accurate channel signaling. Therefore, we must design concurrently the chip, package, and board from the initial spec design of high speed digital system. 이 논문은 PCB의 PDN(Power Distribution Network) 시스템을 고려한 채널 분석을 나타내었다. 설계자가 원하는 PDN 시스템을 설계하기 위하여, 전체 주파수 범위의 PDN이 요구하는 임피던스를 얻는 유용한 설계방법을 제안하였다. 제안된 방법은 주파수 영역과 관계된 계층적 배치 접관방식과 보트와 decoupling 커패시터 사이의 current 흐름의 간섭을 고려한 path-based equivalent 회로를 기본으로 하였다. 비록 빠르고 쉬운 계산을 위한 lumped model일지라도, 실험 결과는 제안된 모델이 numerical 분석처럼 거의 정확함을 보였다. PDN 시스텐의 분석은 패키지 인덕턴스가 파워 노이즈, 데이터 채널을 통한 신호 이동에 영향을 받는다는 것을 보여주고 있으나, 보드 PDN 또한 정확한 채널 신호를 위해 무시할 수 없다는 것을 보여준다. 따라서 설계자는 반드시 초고속 디지털 시스템의 첫 스팩 설계로부터 보드, 패키지, 칩 등을 동시에 디자인을 해야 한다.

초등학교 공간계획을 위한 지역유형분류 및 특성분석 -서울·경기 지역을 중심으로-

이상민,Lee, Sang Min 한국교육녹색환경연구원 2003 교육·녹색환경연구 Vol.3 No.2

This study has the purpose for analysis of each region's property in order to plan a elementary school's space according to community property. For this analysis. we used classification method through classification analysis. classification analysis is one of the useful statistical analysis methode for determining each region's policy through classifying regions which have a similar property. On this study, Seoul and Kyongkido is classified by 4 groups and each group has a different community property. Such a analysis is thought of helping establishing the objective. reasonable space-plan through comparative analysis between subjective claim and objective state indicator of each region.