인간포배기 배아의 효과적인 유리화 동결법의 개발을 위한 연구

이상민,이주희,이상원,이승민,윤산현,임진호,박흠대,이성구,Lee, Sang-Min,Lee, Ju-Hee,Lee, Sang-Won,Lee, Seoung-Min,Yoon, San-Hyun,Lim, Jin-Ho,Park, Huem-Dai,Lee, Seong-Goo 대한생식의학회 2003 Clinical and Experimental Reproductive Medicine Vol.30 No.3

Objective: The purpose of this study was to evaluate the survival rate of vitrified blastocyst according to the freezing vessels, equilibration time in cryoprotectant and artificial dehydration method. Methods: Human blastocysts were vitrified after loading onto the plastic straw, open-pulled straw (OPS), electron microscopy grid (EM grid) for 1.5 min or 3 min. They also were directly plunged into LN2 within 30sec. For artificial shrinkage of blastocysts, 36 gauge fine needle was pushed at the cellular junction of the trophectoderm into the blstocoele cavity until it shrank without damage of inner cell mass. Results: The survival rate of vitrified blastocysts on plastic straw, OPS, EM grid as freezing vessels were 26.7, 13.0 and 60.5%, respectively. The survival rate of EM grid was significantly higher than that of plastic straw and OPS (p<0.05). For 1.5 min equilibrium, the survival rates of early blastocyst (EB), middle blastocyst (MB) and late blastocyst (LB) were 64.4, 81.0, and 20.0% respectively. For 3 min equilibrium, the survival rates of EB, MB, and LB were 69.9, 50.0 and 57.5% respectively. The survival rates of EB and MB were significantly higher than that of LB in 1.5 min equilibrium group (p<0.05), however, the significance was not observed in 3 min equilibrium groups. In cytoplasmic shrinkage before vitrification, the survival rates of EB, MB and LB were 92.9, 100 and 75.9% respectively. The survival rate of MB was significantly higher than that of LB (p<0.05). The survival rates of vitrified blastocysts by artificial dehydration and slow-frozen blastocysts were not significantly different as 88.9 and 66.7%, respectively. Conclusion: This study showed that the vitrification of human blastocysts using EM grid and artificial dehydration is an effective method. Therefore, these methods would be an useful techniques for blastocyst cryopreservation.

헤어리베치(Vice villosa Roth) 품종별 국내 월동성, Biomass 및 무기태 질소 함량비교

이상민,이연,이용환,성좌경,윤홍배,최현석,Lee, Sang-Min,Lee, Youn,Lee, Yong-Hwan,Sung, Jwa-Kyung,Yun, Hong-Bae,Choi, Hyun-Sug 한국유기농업학회 2013 韓國有機農業學會誌 Vol.21 No.1

헤어리베치 8종의 품종별 국내 적응성 검증을 위해 월동률과 biomass, 그리고 토양 중 무기태질소를 조사하였다. 겨울철 월동 후 생존율은 Hungvillosa와 Ostsaat 품종에서 좋았고, Minnie, Oregon common과 TTF1에서 낮았다. 생존율은 biomass에도 영향을 주어서 Hungvillosa와 Ostsaat 품종에서 가장 높았다. 헤어리베치 품종 간 무기성분 농도는 별다른 차이가 관찰되지 않았다. 토양 중 무기태질소 농도는 질소환원능이 좋았던, Hungvillosa와 Ostsaat가 예초 후 10여일 후에 크게 증가시켰다. This study was conducted to evaluate overwintering of hairy vetch (Vicia villosa Roth), biomass, and inorganic N concentration in soil to verify the adaptability of eight cultivars of hairy vetch into country. Winter survival rate was higher for the hairy vetch cultivars, such as Hungvillosa or Ostsaat, than those of Minnie, Oregon common, and TTF1, which affected amount of biomass of each hairy vetch, with higher biomass observed in Hungvillosa or Ostsaat cultivar. There were no significant difference for the mineral nutrients of each hairy vetch cultivar. Inorganic N concentration in soil was increased at 10 days after mowing by application of Hungvillosa and Ostsaat cultivars that had highly produced N content from the raw materials.

녹비작물을 이용한 윤작이 잡초 제어에 미치는 영향

이상민,이병모,이연,이용환,성좌경,윤홍배,최현석,Lee, Sang-Min,Lee, Byung-Mo,Lee, Youn,Lee, Yong-Hwan,Sung, Jwa-Kyung,Yun, Hong-Bae,Choi, Hyun-Sug 한국유기농업학회 2012 韓國有機農業學會誌 Vol.20 No.2

본 시험은 유기농업 파 및 고추재배를 위하여 호밀과 헤어리베치를 겨울에 윤작하여 잡초 발생량 억제에 미치는 효과를 검토하기 위하여 수행되었다. 파 재배구에서 호밀을 토양에 환원하지 않은 처리구에 비해 호밀을 환원하였을 경우 잡초발생량을 52% 경감시켰다. 고추 재배구에서는 그러한 효과가 뚜렷하게 관찰되지 않았다. 파 재배시 어저귀, 참방동사니, 바랭이와 같은 초종은 호밀뿌리 분비물에 의해 발아가 억제되는 경향이었으나 헤어리베치는 그러한 발생억제 효과가 크지 않았다. 파 재배시 호밀 환원은 잡초발생량을 경감시켰고 잡초의 종 다양성 지수를 증가시켰다. This study was conducted to evaluate the effects of crop rotation on the weed occurrence in green onion and red pepper cultivation. Rye and hairy vetch were annually seeded for winter season. The reduction of weed germination was pronounced by about 50% in green onion field, but was not significant in red pepper field through rye cropping. Among weed species, the germination of Abutilon theophrasti, Cyperus iria and Digitaria ciliaris was distinctly inhibited by exudate of rye root, but not by that of hairy vetch in vitro experiment. The return of rye biomass into onion field decreased amount of weed biomass but increased index of weed diversity.

경두개 자기 자극이 인지 기능에 미치는 영향

이상민,채정호,Lee, Sang Min,Chae, Jeong-Ho 대한생물정신의학회 2016 생물정신의학 Vol.23 No.3

Transcranial magnetic stimulation (TMS) is a safe, noninvasive and useful technique for exploring brain function. Especially, for the study of cognition, the technique can modulate a cognitive performance if the targeted area is engaged, because TMS has an effect on cortical network. The effect of TMS can vary depending on the frequency, intensity, and timing of stimulation. In this paper, we review the studies with TMS targeting various regions for evaluation of cognitive function. Cognitive functions, such as attention, working memory, semantic decision, discrimination and social cognition can be improved or deteriorated according to TMS stimulation protocols. Furthermore, potential therapeutic applications of TMS, including therapy in a variety of illness and research into cortical localization, are discussed.

저항 용접을 이용한 금속 샌드위치 판재 접합에 관한 연구 : Part 2 - 공정해석

이상민,김진범,나석주,Lee Sang-Min,Kim Jin-Beom,Na Suck-Joo 대한용접접합학회 2005 대한용접·접합학회지 Vol.23 No.6

In part 1. optimal process parameters such as thickness of stopper and welding time are achieved to produce high strength ISB(Inner Structured and Bonded) panels. Developed process is different from the usual resistance welding process in the number of points welded at a time. In part 2, Numerical modeling for this new process is proposed and the variation of contact area with respect to the gap of electrodes is studied through FE analyses, Besides, it is tried to figure out the welding nugget formation and proper distance between welding points. FE analytic results show that inner structures are melted more than skin plate, and current distribution between points to be welded can be controlled by distance welding points. Comparison of some FE analytic results with corresponding experimental results could confirm the validity of the proposed numerical modeling.

음주특성이 체질량지수 및 혈압에 미치는 영향

이상민,송현주,손의동,Lee, Sang-Min,Song, Hyun-Ju,Sohn, Uy-Dong 대한약학회 2007 약학회지 Vol.51 No.6

To investigate effect of alcohol consumption characteristics on the body mass index (BMI) and blood pressure (BP), we performed statistical analyses of data from questionnaire survey among adults over 20 years old, using SPSS WIN program. Alcohol consumption characteristics such as frequency of drinking, amount and toxic degree of alcohol didn't show a significant correlation with BP. Amount of alcohol consumption and BMI showed a positive correlation (r=.264, p<.01). Waist circumference and BMI had the highest correlations with amount of alcohol consumption, which increased with aging.

학교시설 통합정보시스템 구축방안에 관한 연구 - 정보화 구축설계 방향을 중심으로 -

이상민,노근철,유승호,Lee, Sang Min,Ro, Keun Chul,Yoo, Seung Ho 한국교육녹색환경연구원 2017 교육·녹색환경연구 Vol.16 No.1

학교 시설이 가장 중요한 공공시설 중 하나라는 사실에도 불구하고 학교시설에 대한 정보가 체계적으로 관리되지 않고 있는 실정이다. 이에 본 연구에서는 학교시설 정책의 가장 기본적인 요소인 학교시설 현황에 대한 객관적인 데이터를 체계적으로 확보하기 위한 시스템 구축방향에 대한 연구를 진행하였다. 본 연구에서는 학교 시설 정보 관리 절차 및 학교시설의 정보 자료 관리 수준을 향상시킬 수있는 시스템 구축방향을 제안하였으며, 실무적인 활용이 가능한 서비스 제공을 위한 시스템 구축 설계방향을 제시하였다. Despite the fact that school facilities are one of the most important public facilities, information about school facilities is not systematically managed. The purpose of this study was to investigate the direction of system construction to provide objective data on school facilities, which is the basic element of school facility policy. In this study, we propose a system construction method to improve the information management procedure of school facility information and school facilities, and suggest a system design direction for providing service that can be practically used.

원형정자세포의 주입에 의한 임신과 분만 1례

이상민,정지영,한용태,박해택,박흠대,정길생,이기영,Lee, S.M.,Jung, J.Y.,Han, Y.T.,Park, H.T.,Park, H.D.,Chung, K.S.,Lee, K.Y. 대한생식의학회 1998 Clinical and Experimental Reproductive Medicine Vol.25 No.2

We demonstrated that the normal pregnancy and delivery by round spermatid injection (ROSI) into oocytes was achieved from nonobstructive azoospermia patient. In this case, the normal fertilization rate was 50%. All of the two pronuclear stage embryos cleaved and were transferred to the patient's uterus. A singleton pregnancy was achieved and resulted in the birth of normal female infant. This resuJt show that intracytoplasraic injection of round spermatid seems to be new treatment of nonobstmctive azoospermia male infertility. Further research is needed to evaluate the required culture conditions to induce progression of the round spermatid into a more elongated stage.

교육에서의 소진에 관한 이론적 고찰

이상민,안성희,Lee, Sang Min,An, Sunghee 연세대학교 의과대학 2014 의학교육논단 Vol.16 No.2

Burnout research has mostly been performed in human service fields. However, it has recently been expanded to cover diverse settings and even non-occupational samples, such as students. In this study, we defined the construct of academic burnout, which is the burnout experienced by students. Next, we described the concept of academic burnout by introducing measurements for assessing academic burnout. On the basis of the demand-control model (DCM) and effort-reward imbalance model (ERIM), which are the most predominant theoretical burnout models, we described the causal factors and the pathway to experiencing academic burnout symptoms. The ERIM was a more influential model than the DCM when explaining the academic burnout of Korean students. Based on the results of previous longitudinal studies on academic burnout, we recognized emotional exhaustion and academic inefficacy as the initial symptoms of academic burnout. Finally, we discussed the prevention and intervention programs with specific components that should be included in those programs.

학교시설 수선주기 설정에 관한 연구

이상민,Lee, Sang Min 한국교육녹색환경연구원 2018 교육·녹색환경연구 Vol.17 No.2

본 연구는 안전성, 노후도, 경제성 등 시설 여건을 종합적으로 고려하여 개선이 필요한 중장기적인 사업물량 및 사업예산 규모를 추정할 수 있도록 하기 위해 국가수준의 시설개선 기준(수선주기)을 설정하여 체계적인 시설개선계획 수립을 통한 적정수준의 교육환경 확보에 기여할 수 있도록 하는 것을 주목적으로 한다. 적정 수선주기는 기본적으로 최근 2년간 교육청에서 실제 집행한 교육환경개선사업의 실적자료를 분석하여 직전 보수년도와 사업시행연도를 근거로 수선주기 기초자료를 산출하였다. 이를 근거로 17개 시도교육청 실무자를 대상으로 델파이 조사를 실시하여 시도교육청의 의견을 수렴하였으며 최종 적정 수선주기는 관련규정과 실적자료, 델파이조사 결과를 종합적으로 검토하여 설정하였다. In Korea, it is difficult to secure student health and safe educational environment due to the rapid deterioration of many school facilities built in the 70s and 80s in the past. As a result, through the improvement of school facilities, social needs for safe schools from various disasters, accidents, Is increasing. The purpose of this study was to establish a standard for the establishment of the national and school facilities for the mid - to long - term plan for the improvement of the old school facilities, and to propose a framework for mid - to long - term and rational planning that can be used by the national and local government offices. The purpose of this study is to establish a mid - and long - term plan for the improvement of old school facilities. The appropriate repair cycle for the improvement of the school facilities set up in this study can be utilized as a standard for the mid - and long - term planning for improvement of the school facilities in the Ministry of Education and the School of Education. To this end, a standard model, It is possible to roughly estimate the overall improvement in the mid- to long-term facilities at the national and regional levels.