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연구논문 : 수산폐기물인 미더덕껍질 용매 추출물의 항산화 및 항고혈압 활성
이상미 ( Sang Mee Lee ),이예람 ( Ye Ram Lee ),조광식 ( Kwang Sik Cho ),박수연 ( Soo Yun Park ),장은영 ( Eun Young Jang ),황대연 ( Dae Youn Hwang ),정영진 ( Young Jin Jung ),손홍주 ( Hong Joo Son ) 한국환경과학회 2015 한국환경과학회지 Vol.24 No.7
Styela clava tunic is generated in large amounts as a waste from S. clava processing plants and leads to environmental problems. It destroys the beach scenery and causes a bad smell and pollution by trashing on the seashore. Therefore, purpose of this study was to investigate antioxidant and antihypertensive activities of different solvent extracts from S. clava tunic for recycling of fishery waste. Antioxidant and antihypertensive activities of all extracts were concentration-dependent. Of extracts, hot water extract showed the highest DPPH radical scavenging activity with the lowest effective concentration (EC50) value (0.733 mg/ml). Chloroform extract exhibited the highest metal chelation activity with the lowest EC50 value (2.696 mg/ml). Autoclaved water extract showed the highest NO radical scavenging activity with the lowest EC50 value (0.491 mg/ml) and n-hexane extract showed the highest reducing power (A700=1.897 at 100 mg/ml). And n-butanol extract showed the highest SOD-like activity with the lowest EC50 value (19.116 mg/ml) and ACE inhibition activity with the lowest inhibitory concentration(IC50) value (0.149 mg/ml). These results indicate that extracts obtained from S. clava tunic may potential candidate to reduce diseases caused by various oxidative stresses and hypertension.
이상미(Sang-Mi Lee),이은영(Eun-Young Lee),강애리(Ae-Ri Kang),안영미(Young-Mee Ahn) 한국생활환경학회 2012 한국생활환경학회지 Vol.19 No.6
A cross sectional explorative study was conducted to invest the relationship between the temperature and humidity of the environment that newborns were l℃ated at and the central and peripheral body temperatures of newborns in hospitalized newborns using a primary dataset on 199 high-risk infants. Data was collected on the temperature (℃) and humidity (%) of the environments (incubator, radiant warmer and room), the temperatures (℃) from the central part (right upper quadrant of abdominal surface) and the peripheral part (dorsal surface of hand) of newborns. As well the weight and length was deducted from main dataset with general characteristics of newborns. The result showed that all of newborn revealed the peripheral hypothermia regardless of 88% of them in normothermia, 9% in hypothermia and 3% in hyperthermia. The low humidity of the environment, rather than the low environmental temperature of either incubator or radiant warmer, was mainly responsible for the massive peripheral hypothermia. Proper environmental humidity should be provided to maintain the normothermia of entire body system in high-risk newborns at NICU.
수산폐기물 재활용을 위한 미더덕껍질 용매 추출물의 생리활성 탐색
이상미 ( Sang Mee Lee ),강은진 ( Eun Jin Kang ),고태훈 ( Tae Hun Go ),정성윤 ( Seong Yun Jeong ),박근태 ( Geun Tae Park ),이희섭 ( Hee Sup Lee ),황대연 ( Dae Youn Hwang ),정영진 ( Young Jin Jung ),손홍주 ( Hong Joo Son ) 한국환경과학회 2014 한국환경과학회지 Vol.23 No.1
Styela clava tunic is generated in large amounts as a waste from S. clava processing plants and causes environmental problem. Although biological activities of S. clava were reported by many investigators, study on S. clava tunic was little. In this study, therefore, tyrosinase inhibition and antioxidative activities of extracts from S. clava tunic using different solvent were investigated for recycling of the fishery waste. Among extraction methods tested, autoclaved extraction (25.7%) and hot water extraction (18.2%) appeared to be effective for extraction. The highest total phenolic content was 46.6 mg/g in autoclaved extract while the highest flavonoid content was 23.0 mg/g in chloroform extract. All extracts possessed tyrosinase inhibition activity and the inhibition activity was concentration-dependent. Inhibition concentration (IC50) against tyrosinase activity was 0.36×104 mg/ml in ethanol extract, 0.11×103 mg/ml in acetone extract and 0.27 mg/ml in n-butanol extract. Among extracts tested, hot water and autoclaved extracts displayed higher antioxidative activity than organic solvent extracts. Therefore, our data suggest that extract from S. clava tunic may potential candidate for cosmetic product with whitening effect and medicine for diseases caused by various oxidative stresses.
Bacillus amyloliquefaciens를 이용하여 콩 종류와 발효온도를 달리하여 제조한 청국장의 특성
이나리,고태훈,이상미,홍창오,박규민,박근태,황대연,손홍주,Lee, Na-Ri,Go, Tae-Hun,Lee, Sang-Mee,Hong, Chang-Oh,Park, Kyu-Min,Park, Geun-Tae,Hwang, Dae-Youn,Son, Hong-Joo 한국미생물학회 2013 미생물학회지 Vol.49 No.1
This study was carried out to investigate the characteristics of Chungkookjang depending on different soybeans and fermentation temperatures using Bacillus amyloliquefaciens C2 isolated from homemade Chungkookjang. The highest protease activity was 854 U/g in yellow soybean Chungkookjang and 847 U/g in black soybean Chungkookjang at $35^{\circ}C$. The highest amylase activity was 3.87 U/g at $40^{\circ}C$ in yellow soybean Chungkookjang and 4.96 U/g at $45^{\circ}C$ in black soybean Chungkookjang. The highest reducing sugar content was 16.11 mg/g at $40^{\circ}C$ in yellow soybean Chungkookjang and 19.08 mg/g at $45^{\circ}C$ in black soybean Chungkookjang. The highest amino type nitrogen content was 420 mg%/g in yellow soybean Chungkookjang and 194 mg%/g in black soybean Chungkookjang at $40^{\circ}C$. The highest pH was 7.92 at $40^{\circ}C$ in yellow soybean Chungkookjang and 7.59 at $45^{\circ}C$ in black soybean Chungkookjang. The highest number of viable cell was 9.3 log CFU/g at $40^{\circ}C$ in yellow soybean Chungkookjang and at $35^{\circ}C$ in black soybean Chungkookjang. On the other hand, the lowest ammonia type nitrogen content was 225 mg%/g at $45^{\circ}C$ in yellow soybean Chungkookjang and 80 mg%/g at $40^{\circ}C$ in black soybean Chungkookjang. Yellow soybean Chungkookjang showed high protease activity, pH and amino type nitrogen, whereas black soybean Chungkookjang showed high amylase activity and reducing sugar. 본 연구에서는 가정에서 제조한 청국장으로부터 순수분리한 Bacillus amyloliquefaciens C2를 이용하여 콩 종류와 발효온도에 따른 청국장의 품질 특성 변화를 조사하였다. Protease 활성은 $35^{\circ}C$에서 배양했을 때 황태 청국장에서 854 U/g, 흑태 청국장에서 847 U/g로 가장 높았다. Amylase 활성은 황태 청국장의 경우, $40^{\circ}C$에서 3.87 U/g, 흑태 청국장은 $45^{\cricr}C$에서 4.96 U/g로 가장 높았다. 환원당 함량은 황태 청국장의 경우, $40^{\circ}C$에서 16.11 mg/g, 흑태 청국장은 $45^{\circ}C$에서 19.08 mg/g으로 가장 높았다. 아미노태 질소 함량의 경우, 황태 청국장은 $40^{\circ}C$에서 420 mg%/g, 흑태 청국장은 194 mg%/g로 가장 높았다. pH의 경우, 황태 청국장은 $40^{\circ}C$에서 7.92, 흑태 청국장은 $45^{\circ}C$에서 7.59로 가장 높았다. 황태 및 흑태 청국장의 생균수는 각각 $40^{\circ}C$ 및 $35^{\circ}C$에서 9.3 log CFU/g로 가장 높았다. 반면에 암모니아태 질소 함량은 황태 청국장의 경우, $45^{\circ}C$에서 225 mg%/g, 흑태 청국장은 $40^{\circ}C$에서 80 mg%/g로 가장 낮았다. 황태 청국장은 높은protease 활성, pH 및 아미노태 질소함량을 나타내었다. 반면에, 흑태 청국장은 높은 amylase 활성과 환원당 함량 및 낮은 암모니아태 질소 함량을 나타내었다.
김경미 ( Gyung Mee Gim ),문지혜 ( Jih Ye Moon ),정순진 ( Sun Jin Jeong ),이상미 ( Sang Mi Lee ) 한국농촌지도학회 2013 농촌지도와 개발 Vol.20 No.4
이 연구는 최근 네덜란드와 벨기에 등 농업분야 선진국에서 관심과 투자가 증대되고 있는 치유농업(Agro-healing)의 개념을 정립하였고, 우리나라 치유농업의 현황 및 특성 분석을 통해 발전 방안을 제시하였다. 연구목적 달성을 위해 치유농업 선진국의 정책과 제도, 조직, 관련 법, 인력 등에 대한 문헌분석(내용분석)을 하였으며, 농업, 건강, 교육, 원예 및 동물 매개 치료, 민간, 농업정책 집행 분야별 전공 교수, 연구원, 대표 및 담당자 9인으로 구성된 전문가 포커스 그룹 인터뷰를 진행하였다. 또한 치유농업 분야 종사자 165명을 대상으로 설문조사를 실시하였다. 연구결과 우리나라 치유농업의 개념은 ‘농업·농촌자원(식물, 동물, 농촌환경, 농촌문화) 또는 이와 관련된 활동 및 산출물을 통하여 모든 국민의 심리적·사회적·신체적·인지적 건강을 도모하는 산업 및 활동’으로 정의하였으며 정립된 개념과 연계하여 치유농업의 목적, 자원, 대상을 설정하였다. 또한 우리나라 치유농업의 정착과 발전을 위한 제언을 제시하였다. This study investigated definition of care farming in which advanced countries in agricultural industry such as Netherlands and Belgium increase their interest and investment, and established a strategy for settlement and development of care farming industry in Korea. For achieving purpose of the study, literature review was implemented to analyze policy, system, related law, workforce of advanced countries in care farming, and experts` conference which consists of 9 representatives such as professors, researchers in areas of agriculture, health, education, horticulture, animal-assisted therapy was implemented. Also survey was conducted targeting 165 people of practitioners in care farming industry. For the result, the concept of care farming in Korea was defined as ‘Industry and activity which promotes psychological·social· physical·cognitive health of every Korean people by utilizing agricultural resources(plants, livestock, rural environment, rural culture) or related activity and output’, and the purpose, resources, targets of care farming was determined by the definition.