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면역결핍바이러스 인테그라제 억제제로서 Baicalein과 Baicalin
이민전(Min Jun Lee),김미라(Mi Ra Kim),이용섭(Yong Sup Lee),신차균(Cha Gyun Shin) 대한약학회 2003 약학회지 Vol.47 No.1
Baicalein and baicalin are flavonoid compounds isolated from medicinal herb Scutellaria baicalensis Georgi (Labiatae) and have been known to possess antiviral activities. In the present study; we investigated the in vitro effects of baicalein and baicalin on the three distinctive enzymatic activities of the human immunodeficlency virus type-1 (HIV-1) integrase - endonucleolytic, integration, and disintegration activities. Both compounds inhibited the three enzymatic activities in a dose-dependent manner The 50% inhibitory concentrations of baicalein and baicalin for endonucleolytic activities of HIV-1 integrase were 4.4 +/- 3.3 and 25.9 +/- 4.0μM, respectivel. In general, baicalein exhibited nearly 6- to 10-fold stronger inhibition than baicalin for the three enzymatic activities. These data demonstrate that baicalein or baicalin can be used as a leading compound to develop anti-AIDS chemotherapeutic agents targeting to the HIV-1 integrase.
알루미늄 다공성소재를 이용한 RFID Tag용 열차폐부품 개발
방제오,이효수,정택균,이민하,김범성,정승부,Bang, Jae-Oh,Lee, Hyo-Soo,Jung, Taek-Kyun,Lee, Min-Ha,Kim, Bum-Sung,Jung, Seung-Boo 한국분말야금학회 2011 한국분말재료학회지 (KPMI) Vol.18 No.2
The RFID (Radio-Frequency Identification) is an automatic identification method, relying on storing and remotely retrieving data using devices called RFID tags or transponders. Some RFID tags have been used in severe environment of temperature ranged from $200^{\circ}C$ to $250^{\circ}C$ for a long time and may cause serious problems such as signal error, short life cycle and explosion. Conventionally, the RFID tags for high temperature applications consisted of Fe-alloy housing part, ceramic powder and RFID sensor. However, it has disadvantage of heavy weight, signal noise and heat shield capability. In this study, we newly applied the aluminum porous materials fabricated by polymer leaching process into RFID tags in order to improve heat shielding ability, and compared the properties of RFID tag inserted by aluminum porous with the conventional one.
Jinwook Seo,Hak Sung Lee,이민전,Mira Kim,신차균 대한약학회 2004 Archives of Pharmacal Research Vol.27 No.1
DA-125, a novel derivative of adriamycin, is known for its anti-cancer activity. In this study, the inhibitory mechanism of DA-125 on topoisomerase was investigated in the simian virus 40 (SV40) replicating CV-1 cell by studying the SV40 DNA replication intermediates and DNAtopoisomerase complexes. DNA-protein complexes that were formed in the drug-treated cells were quantitated by using a glass filter assay. SV40 DNA replication intermediates that were accumulated in the drug-treated CV-1 cell were analyzed in a high resolution gel. DA-125 did not accumulate B-dimers of SV40 DNA replication intermediates which were found in the adriamycin- treated CV-1 cells. DA-125 induced a dose-dependent formation of the DNA-protein complexes, while adriamycin did not. When adriamycin and etoposide (VP16) were added to the SV40-infected cells at the same time, adriamycin blocked the formation of the DNA-protein complexes induced by VP16 in a dose-dependent manner. However, DA-125 blocked the formation of the DNA-protein complexes induced by VP16 up to the maximum level of the DNA-protein complexes that were induced by DA-125 alone. Adriamycin and DA-125 did not inhibit the formation of the DNA-protein complexes that were caused by camptothecin, a known topoisomerase I poison. DA-125 is bifunctional in inhibiting topoisomerase II because it simultaneously has the properties of the topoisomerase II poison and the DNA intercalator. As a topoisomerase II poison, DA-125 alone induced dose-dependent formation of the DNA-protein complexes. However, as a DNA intercalator, it quantitatively inhibited the formation of the DNA-protein complexes induced by a strong topoisomerase II poison VP16. Furthermore considering that the levels of the DNA-protein complex induced by VP16 were decreased by DA- 125 in terms of the topoisomerase II poison, we suggest that DA-125 has a higher affinity to the drug-binding sites of DNA than VP16 has.
단기간의 체중 감량이 고등학교 태권도 선수의 체력, 등속성 각근력 및 혈액성분에 미치는 영향
김용규(Kim, Yong-Gyu),이민균(Lee, Man-Gyoon) 경희대학교 스포츠과학연구원 2009 태권도과학 Vol.2 No.1
본 연구의 목적은 단기간의 체중 감량이 고등학교 태권도 선수의 체력, 등속성 각근력 및 혈액성분에 미치는 영향을 규명하는 것이었다. 18명의 태권도 선수가 9명의 체중감량집단과 9명의 통제집단에 무선할당 되었다. 체중감량집단의 선수는 96시간 내에 체중의 5% 이상을 감량한 반면, 통제집단의 선수는 동일한 처치 기간 동안 평상시 생활을 그대로 유지하였다. 체력, 등속성 각근력, 그리고 다양한 혈액성분을 처치 전·후에 측정하여 비교하였다. 본 연구에서 얻은 주요 결과는 다음과 같다. 첫째, 체중과 체지방률은 체중감량집단에서만 유의하게 감소되었다. 둘째, 좌측 악력을 제외한 모든 체력요소에서 두 집단 모두 유의한 변화가 나타나지 않았다. 셋째, 등속성 각근력과 관련하여 굴근의 경우 체중감량집단에서만 감소되는 경향을 보였으나, 통계적으로 유의한 변화는 나타나지 않았다. 넷째, 혈당, Na+, 그리고 K+에서 두 집단 모두 유의한 변화가 나타나지 않았지만, 총단백질량, Ca2+, P+, 자유지방산, Mg+, 그리고 이온화 칼슘에서 유의한 변화가 나타났다. 단기간 이루어진 체중의 감소에 따라 체력의 감소가 유의하게 나타나지 않았으나 등속성 각근력과 혈액성분에 부정적인 영향을 줄 수 있는 것으로 나타나 단기간보다는 장기간에 걸쳐 체계적으로 체중 감량을 수행하는 것이 바람직할 것으로 판단된다. The study was designed to examine the effects of short-term weight loss on physical fitness, isokinetic leg strength, and blood variables in male high school taekwondo players. Eighteen taekwondo players were randomly divided into two groups, i.e.. weight loss group(WL: n=9) and control group(CON: n=9). The subjects in WL were asked to lose at least 5% of their body weight in 96 hours, whereas the subjects in CON were asked not to change their normal life pattern. Physical fitness, isokineetic leg strength, and blood variables were measured before and after the interventions and were compared by group and test. The results were as follows : 1. Body weight and percent body fat decreased significantly in WL, whereas no changes were found in CON. 2. Left grip strength decreased significantly in WL, whereas no changes were found in the variables in CON. There were not significant changes in other physical fitness components in both groups. 3. In variables regarding to isokinetic leg strength, results in flexion showed a trend to be decline in WL, however, no significant changes were found in extension. 4. There were no significant changes in blood glucose, Na+, and K+ in both groups. Total protein, Ca2+, P+, free fatty acid, Mg+, and ionized calcium showed different changes between the two groups. The short-term weight loss did not result in serious decrease in physical fitness, but it would be possible the short-term weight loss may have negative effects on the balance of minerals.
지속성 외래 복막 투석 환자에서 복막 투석액 및 혈청 Cancer Antigen 125 (CA125)농도 변화의 임상적 의의
조영욱 ( Young Wook Cho ),우학 ( Hak Woo ),배우균 ( Woo Kyun Bae ),이윤철 ( Yoon Chul Lee ),이민구 ( Min Goo Lee ),이연경 ( Yun Kyung Lee ),조민석 ( Min Seok Cho ),마성권 ( Seong Kwon Ma ),김수완 ( Soo Wan Kim ),김남호 ( Nam Ho K 대한내과학회 2005 대한내과학회지 Vol.68 No.3
Oct4 단백질의 DNA - 결합 영역에 융합된 HIV - 1 integrase
이민전,서진욱,김미라,신차균 中央大學校 遺傳工學硏究所 2002 遺傳工學硏究論集 Vol.15 No.1
Human immunodeficience virus type-1 (HIV-1) integrase (IN) mediates integration of viral DNA into the host cell genome which is an essential step during the life cycle of retroviruses. HIV-1 IN shows four enzymatic activities in vitro: non-specific DNA- binding, endonucleolytic (3'-processing), integration (strand transfer), and disintegration activities. Oct4 as a transcription factor recognizes and binds the specific sequences containing 5' -ATGCAAAT-3'. In order to study the specific binding and enzymatic activities of the integrase protein, we constructed the four different fusion proteins (OctIN1, OctIN2, INKOct1, INKOct1) using different combination of HIV-1 IN and Oct4 protein. The fusion proteins were overexpressed in Escherichia coli and purified by Ni-NTA and SP-sepharose columns. Analysis of the enzymatic activities showed that all the four fusion proteins has detectable endonucleolytic and integration activities, suggesting that the fusion proteins can be used to study derected integration and sequence specific DNA-binding of the integrase fusion protein.