안티포렌식을 역적용한 모바일 개인정보보호에 관한 기법 연구

이규춘(Lee Kyu Chun),백재환(Baek Jae Hwan),신교욱(Sin Kyo Uk) 한국통신학회 2011 한국통신학회 학술대회논문집 Vol.2011 No.6

원발병소가 갑상선인 전이성 두개골 종양 2례 - 증례보고 -

강한석,박용석,이영배,이규춘,목진호,김한식,Kang, Han Sug,Park, Yong Seok,Lee, Young Bae,Lee, Kyu Chun,Mok, Jin Ho,Kim, Han Sik 대한신경외과학회 2000 Journal of Korean neurosurgical society Vol.29 No.10

The authors present two cases of lytic skull metastasis from thyroid carcinoma. The first case is a 62-year-old female who was diagnosed as thyroid cancer one year ago. She complained a mass over the right parietal area but showed no neurological abnormalities. The mass didn't invade the dura, and was completely removed. Histopathological examination revealed the insular thyroid carcinoma, composed of undifferenciated cells that were responded to thyroglobulin in immunohistochemical staining. The second case is a 75-year-old female who complained a mass over the right parietal and neck area without any neurological abnormality. The mass was confined to the epidural region which was associated with osteolytic change of skull. It was also completely removed. Histopathological examination of mass revealed the follicular thyroid carcinoma.

Lipoxygenase 및 Cyclooxygenase Inhibitor가 뇌수막종세포의 성장에 미치는 영향

박용석,구태헌,이정훈,이영배,이규춘,목진호,김한식,Park, Yong Seok,Koo, Tae Heon,Lee, Jung Hoon,Lee, Young Bae,Lee, Kyu Chun,Mok, Jin Ho,Kim, Han Sik 대한신경외과학회 2000 Journal of Korean neurosurgical society Vol.29 No.1

Object : To verify the effect of the lipoxygenase inhibitor and cycloxygenase inhibitor on meningioma cell proliferation. Method : Using two meningioma cell lines, cell proliferation was determined at 96 hrs after adding inhibitor (AA861, Nordihydroguaiaretic acid(NDGA), Indomethacin, acetyl-11-keto-beta-boswellic acid(AKBA) into medium by methyl tetrazolium salt/phenazine methosulfate(MTS/PMS) non-radioactive cell proliferation assay. We checked optical density with 490nm wavelength UV and this value was used as a proliferative index. The percent of inhibition was also calculated from this value. Conclusion : Indomethacin and NDGA showed no effect on meningioma proliferation. AA861 also showed no significant inhibitory effect, but AKBA demonstrated a significant inhibitory effect on meningioma cell proliferation.

두부외상 후 발생한 지주막하 출혈에 대한 임상분석

구태헌,김한식,목진호,이규춘,박용석,이영배,Goo, Tae Heon,Kim, Han Sik,Mok, Jin Ho,Lee, Kyu Chun,Park, Yong Seok,Lee, Young Bae 대한신경외과학회 2000 Journal of Korean neurosurgical society Vol.29 No.1

Objective : Many authors suggest that patients with traumatic subarachnoid hemorrhage(tSAH) visible on first CT after heve injury had a significantly worse prognosis than patients who do not. The aim of this study is to identify patients with tSAH who present with a bad prognosis by reviewing their clinicoradiological features and plan appropriate treatments. Patients and Methods : We reviewed and analysed the factors that influenced discharge outcomes in 172 patients with tSAH for a 3-year period. The outcome was divided into good(good recovery and moderate disability of glasgow outcome scale) and good(severe disability, vegetative state and death). Results : A regression analysis of statistical significant factors(p<0.05) among the clinical and CT features ranked them by descending order of contribution to Glasgow Outcome Scale(GOS) scores at the time of discharge from acute hospitalization as follows 1) clinical : admission Glasgow Coma Scale(GCS), hypotension, CT grade, abnormal APTT, skull fracture, hyperglycemia(>160mg/dl), hypoxia, operation, 2) CT : basal cistern effacement(BCE), mass lesion, cortical sulcal effacement(CSE), midline shift. Conclusion : We have also experienced that the CT grading scale proposed by Green et al is a simple and useful prognostic factor. The authors believe that the patients with high CT grade need adjuvant therapies as of well surgery but it seems mandatory to consider early identification and correction of hypotension, hyperglycemia, and hypoxia in emergency setting.

아이폰 모바일 메신저 분석을 통한 디지털 포렌식 기법 연구

백재환(Baek, Jae Hwan),이규춘(Lee, Kyu Chun) 한국통신학회 2010 한국통신학회 학술대회논문집 Vol.2010 No.6

Helicobacter pylori 감염 환자에서 Western blot 법에 의한 혈청내 세포독성 유전자의 발현에 관한 연구

김대인,이구,서정일,이창우,김정란,하경임,이규춘,남경수,양창헌,Kim, Dae-In,Lee, Goo,Shu, Jung-Ill,Lee, Chang-Woo,Kim, Jung-Ran,Ha, Gyoung-Yim,Lee, Kyu-Chun,Nam, Kyung-Soo,Yang, Chang-Heon 한국생명과학회 2000 생명과학회지 Vol.10 No.6

The gastric pathogen Helicobacter pylori(H. pylori) establishes long-term chronic infection that can lead to atrophic gastritis, intestinal metaplasia, and gastric cancer. H. pylori, which express cytotoxic genes is now recohnized as a cause of peptic ulcer and is also a major risk factor for the development of gastric adenocarcinoma. We performed this study 1) to assess the detection rate of H. pylori according to direct investigation of bacteria of gastric biopsy specimen and two serologic tests of GAP test and Helico blot 2.0 system in the symptomatic and non-symptomatic group 2) to evaluate and compare the efficacy of two serologic tests of GAP test and Helico blot 2.0 system for the diagnosis of H. pylori infection. Forty-nine patients were positive for H pylori infection based on direct investigation of bacteria by histology. The detection rates of H. pylori infection based on direct investigation of bacteria by histology. The detection rates of H. phlori were significantly lower in gastric cancer than in other gastroduodenal disease(p<0.05). The concordance of two serologic tests of GAP test and Helico blot 2.0 system is poor. There was no statistically significant difference between the expression rate of CagA and VacA in the symptomatic and non-symptomatic group. Although Helico blot 2.0 system may not displace GAP test, it was a very sensitive serologic test for the diagnosis of H. pylori infection and it was used to detect IgG antibodies to H. pylori-specific antigens, including CagA, VacA and the various urease subunit. Our data suggest that further investigation is needed to determine whether or not the serologic expression of cytotoxic gene may be clinical usefulness of diagnostic methods in the gastroduodenal disease.

급성 거미막하 출혈의 진단: FLAIR MR영상과 조영전 CT와의 비교

최원진,최대섭,김정혜,김순,이현경,오연희,김승현,이성우,김욱년,이규춘,Choi, Won-Jin,Choi, Dae-Seob,Kim, Joung-Hae,Kim, Soon,Lee, Hyeon-Kyeong,Oh, Yoen-Hee,Kim, Seung-Hyeon,Lee, Sung-Woo,Kim, Wook-Nyeon,Lee, Kyu-Chun 대한자기공명의과학회 2001 Investigative Magnetic Resonance Imaging Vol.5 No.2

목적 : 급성 거미막하 출혈의 진단에 있어 CT와 비교하여 fluid-attenuated inversion-recovery (FLAIR) MR영상의 유용성을 평가하고자 하였다. 대상 및 방법 : 증상 발생 3일 이내에 비조영 CT와 FLAIR MR영상을 모두 얻었던 28명의 급성 지주막하 출혈 환자를 대상으로 하였다. 거미막하 공간을 피질구, 실비우스열구, 기저조, 후두와의 4부위로 나누어 각 부위 별로 CT와 FLAIR영상에서 출혈이 보이는 정도를 0(출혈 없음), 1(출혈 의심), 2(뚜렷한 출혈)의 점수로 구분하여 평가하였다. 또한 이들 28명 환자와 대조군 35명의 FLAIR영상을 비교하여 거미막하 공간의 고신호 강도를 기준으로 출혈의 유무를 판정할 경우, FLAIR영상의 민감도, 특이도 및 진단적 정확도를 평가하였다. 결과 : FLAIR영상에서 급성 거미막하 출혈은 모든 환자에서 뇌척수액이나 뇌 실질보다 고신호 강도를 보여 100%의 발견율을 보였다. CT와 비교하여 피질구($1.11{\pm}0.80$ vs $0.70{\pm}0.83$: p(0.05)와 후두와($1.41{\pm}0.74$ vs $0.78{\pm}0.80$: p(0.05)에서 FLAIR영상이 CT보다 통계적으로 유의하게 우수하였다 FLAIR영상에서 거미막하 공간의 고신호 강도를 기준으로 거미막하 출혈 유무를 평가한 결과에서 100%의 민감도, 특이도 및 진단적 정확도를 보였다. 결론 급성 거미막하 출혈의 진단에 FLAIR MR영상은 매우 유용하며, 특히 출혈의 양이 소량인 경우와 후두와의 출혈을 진단하는데 CT보다 우수하다. Purpose : Our aim was to evaluate the usefulness of fluid-attenuated inversion recovery (FLAIR) MR imaging for detection of acute subarachnoid hemorrhage (SAH) compared with unenhanced CT. Materials and methods ; We compared FLAIR MR images with unenhanced CT scans in 28 patients with acute SAH. Findings of SAH on CT and MR images were graded as 0 (absence), 1 (suspicious), 2 (definite) in the cerebral sulci, sylvian fissure, basal cistern, and cisterns of the posterior fossa. We also compared FLAIR MR images of 28 patients with those of 35 normal subjects, and then the sensitivity, specificity, and diagnostic accuracy of FLAIR MR image for detection of acute SAH were calculated. Results : FLAIR MR image was superior to CT in detecting SAH in the posterior fossa ($1.41{\pm}0.74{\;}vs{\;}0.78{\pm}0.80$; p<0.05) and cortical sulci ($1.11{\pm}0.80{\;}vs{\;}0.70{\pm}0.83$; p<0.05). There was no significant difference between FLAIR MR image and CT in detecting SAH in the basal cistern and sylvian fissure. The sensitivity, specificity, and diagnostic accuracy of FLAIR MR image for detection of SAH were 100% in all. Conclusion : FLAIR MR image is useful in detecting acute SAH, especially in patients with small amount of SAH or SAH in the posterior fossa.

위암 및 위암의 전구 병변에서 p16INK4A 촉진자의 과메칠화

김남일 ( Nam Il Kim ),김대인 ( Dae In Kim ),김성자 ( Sung Ja Kim ),이구 ( Goo Lee ),서정일 ( Jung Il Suh ),이창우 ( Chang Woo Lee ),장태정 ( Tae Jung Jang ),김한식 ( Han Sik Kim ),이규춘 ( Kyu Chun Lee ),최명규 ( Myung Gyu Choi ) 대한소화기학회 2002 대한소화기학회 춘계학술대회 Vol.2002 No.-

<목적> 위암에서 p16INK4A 유전자의 불활성화는 p16INK4A 촉진자의 과메칠화에 의한 불활성화가 중요한 기전으로 보고되었지만 현재까지 위암 주변 조직의 p16INK4A 촉진자의 과메칠화와 위암의 전구 병변에 대한 연구는 없는 실정이다. 따라서 본 연구는 만성위염, 위선종, 위암과 위암에 인접한 비종양성 조직에서 p16INK4A 촉진자의 메칠화를 조사하고 p16INK4A 단백질의 발현을 분석하여 위암의 다단계 발생에서 p16INK4A 촉진자 과메

위암종 및 위암종의 전구병변에서 p27Kip1 의 발현

김남일(Nam Il Kim),강혁주(Hyeock Joo Kang),정희철(Hee Chul Jung),김성자(Sung Ja Kim),이구(Goo Lee),서정일(Jeong Il Suh),이창우(Chang Woo Lee),이규춘(Kyu Chun Lee),김한식(Han Sik Kim),장태정(Tae Jung Jang),양창헌(Chang Heon Yang) 대한내과학회 2002 대한내과학회지 Vol.62 No.4

목적 : 위암종은 한국인 남자에서 가장 흔한 악성 종양으로 알려져 있으며 위암종 환자의 예후를 결정하는 요인에 관한 많은 연구가 있어왔다. 또한 사람의 여러 종양에서 CDKI 중 하나인 p27Kip1의 소실이 암의 성장과 연관이 있다고 알려져 있다. 이에 위암종, 위선종 그리고 위암종의 전구병변에서 p27Kip1에 대한 면역조직화학적 염색을 시행하여 p27Kip1의 발현정도를 조사하고 지금까지 알려져 있는 요인들과의 상관관계를 살펴보아 예후인자로서 유용하게 쓰일 수 있을 지에 대해 알아보고자 하였다. 방법 : 위암종으로 진단 받고 위절제술을 시행 받은 진행성 위암종 43예, 조기 위암종 19예, 위선종 22예, 위암인접 비종양조직 110개 및 조직학적으로 염증이 없거나 거의 미미한 정상군 10예를 대상으로 하였으며 위암인접 비종양조직은 다시 만성위염 32개, 장형화생 29개 그리고 위암종에서 1 cm 이내의 범위에 있는 이행상피 49개로 나누어 면역조직화학 염색을 이용하여 p27Kip1의 발현정도를 확인하였다. 결과 : 위암종에서 위암인접 비종양 조직과 위선종에 비해 p27Kip1의 발현이 유의하게 감소하였고 (p<0.05), 위암인접 비종양 조직은 정상군보다 p27Kip1의 발현이 감소하였으며 위암인접 비종양조직중 이행상피군이 만성위염과 장형화생보다 p27Kip1 발현이 감소되었다 (p<0.05). 위암종에서 진행성 위암종이 조기 위암종보다 p27Kip1 발현이 감소하였으며 (p<0.05), 미만형 위암종이 장형 위암종보다 p27Kip1 발현이 감소하였다 (p<0.05). p27Kip1 발현의 감소는 림프절 전이와 관련이 있었지만 (p<0.05), 원격전이와는 관련이 없었다 (p>0.05). 결론 : p27Kip1 발현이 위암종의 다단계 발생에서 초기에 감소됨을 알 수 있었고 위암종의 진행 및 분화와 연관성이 있었지만 예후인자로서의 가치는 더 많은 연구가 필요할 것으로 사료되었다. Background : The cyclin-dependent kinase inhibitor p27Kip1 is a negative regulator of cell cycle progression at G1/S transition. Recently, the expression level of p27Kip1 was decreased in many cancers such as breast, pituitary gland, colon and stomach. We studied the expression of p27Kip1 in gastric cancers, precancerous lesions and normal gastric tissues and analysed its correlation to clinicopathologic data including tumor differentiation, tumor depth, nodal and distant metastasis in gastric cancers. Methods : p27Kip1 were immunohistochemically stained in the tissue specimens of 62 resected cancers, 110 corresponding adjacent non-neoplastic tissues, 22 gastric adenomas and 10 normal gastric tissues. Adjacent non-neoplastic tissues consisted of 32 chronic gastritis, 29 intestinal metaplasia and 49 transitional mucosa. Results : Gastric cancers showed significantly decreased expression level of p27Kip1 when compared with non-neoplastic lesions and adenomas. Labeling index of p27Kip1 were more decreased in chronic gastritis, intestinal metaplasia and transitional mucosa than in normal mucosa. Early gastric cancers showed significantly decreased expression level of p27Kip1 when compared with advanced gastric cancers. In gastric cancers, p27Kip1 labeling index was significantly decreased in diffuse type and presence of nodal metastasis however did not show relationship with distant metastasis and tumor depth of advanced gastric cancers. Conclusion : We suggest that p27Kip1 may be decreased in the early stage of gastric carcinogenesis and play an important role in the progression and differentiation of gastric cancers. More further studies are thought to be necessary in order to evaluate its prognostic factor in gastric cancers.(Korean J Med 62:396-404, 2002)

혈전용해제 투여후 발생된 뇌내출혈에 대한 임상분석

이규춘,김한식,목진호,박용석,이영배,어경윤 東國大學校 2000 東國論叢 Vol.39 No.-

Early thrombolysis with intravenous urokinase may be beneficial for patients with acute ischemic stroke. But thrombolytic agent (urokinase, tissue plasminogen activator) can be one of the causes of spontaneous intracerebral hemorrhage. ICH related thrombolytic agents represent low incidence and slow progression. This slow but prolonged process of bleeding could produce massive hematoma and higher mortality. The mechanism of hemorrhagic transformation is reperfusion through the already damaged vessel. Because of increasing of thrombolytic agents for recanalization of cerebral ischemia and transient ischemic attack in recently, the importance of possible ICH related to these agents need to be reemphesized. The authors analysed urokinase related 9 patients of spontaneous ICH 361 patients to evaluate the underlying mechanism, their character,s the factors with influence the formation of ICH and prognosis through the medical record, operation record, and brain CT. In this study, the mean duration between drug administration and hemorrhagic attack was 15hours 2 minuts(with range from 80 minuts to 45 hours). The meam volume of ICH was 46.3cc. Brain CT revealed that 2 cases were multiple hematoma and 3 cases were combined with intraventricular hematoma. Both multiple ICH and ventricular hematoma were bad results. Three cases were treated conservatively and six cases were operated with open caniotomy. The 78% cases of treatment patients were bad outcome(severe disability: 1case, vegetative state: 1 case, death: 4cases). In this study, the prolonged prothrombine time did not useful predictable value. Although we could not find definitive factors to influence the prognosis, the mortality rate of multiple hematoma and combined ventricular hematoma were much higher than single hematoma without ventricular hemorrhage. In our cases, systolic pressure was increased above 160mmHg in 5cases(56%). Maybe arterial hypertension is risk factor of hemorrhage. Therefore, thrombolytic drugs during the acute phase of cerebral ischemia should be used carefully both in patients with multiple ICH and hypertensive old ages.